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Endotoxin-induced inflammation disturbs melatonin secretion in ewe
Andrzej Przemyslaw Herman,Karolina Wojtulewicz,Joanna Bochenek,Agata Krawczynska,Hanna Antushevich,Bartosz Pawlina,Marlena Zielinska-Gorska,Anna Herman,Katarzyna Romanowicz,Dorota Tomaszewska-Zaremba 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: The study examined the effect of intravenous administration of bacterial endotoxin—lipopolysaccharide (LPS) —on the nocturnal secretion of melatonin and on the expression of enzymes of the melatonin biosynthetic pathway in the pineal gland of ewes, taking into account two different photoperiodic conditions: short-night (SN; n = 12) and long-night (LN; n = 12). Methods: In both experiments, animals (n = 12) were randomly divided into two groups: control (n = 6) and LPS-treated (n = 6) one. Two hours after sunset, animals received an injection of LPS or saline. Blood samples were collected starting one hour after sunset and continuing for 3 hours after the treatment. The ewes were euthanized 3 hours after LPS/saline treatment. The concentration of hormones in plasma was assayed by radioimmunoassay. In the pineal gland, the content of serotonin and its metabolite was determined by HPLC; whereas the expression of examined genes and protein was assayed using real-time polymerase chain reaction and Western Blot, respectively. Results: Endotoxin administration lowered (p<0.05) levels of circulating melatonin in animals from LN photoperiod only during the first hour after treatment, while in ewes from SN photoperiod only in the third hour after the injection. Inflammation more substantially suppressed biosynthesis of melatonin in ewes from SN photoperiod, which were also characterised by lower (p<0.05) cortisol concentrations after LPS treatment compared with animals from LN photoperiod. In the pineal gland of ewes subjected to SN photoperiod, LPS reduced (p<0.05) serotonin content and the expression of melatonin biosynthetic pathway enzymes, such as tryptophan hydroxylase and arylalkylamine-N-acetyltransferase. Pineal activity may be disturbed by circulating LPS and proinflammatory cytokines because the expression of mRNAs encoding their corresponding receptors was determined in this gland. Conclusion: The present study showed that peripheral inflammation reduces the secretion of melatonin, but this effect may be influenced by the photoperiod.
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Pratt, Kenneth W,Ortiz-Aparicio, Jose Luis,Matehuala-Sanchez, Francisco Javier,Pawlina, Monika,Kozlowski, Wladyslaw,Borges, Paulo P,da Silva Junior, Wiler B,Borinsky, Mó,nica B,Hernandez-Mabel P Springer-Verlag 2013 Metrologia Vol.50 No.-
<P>This key comparison (KC), CCQM-K73, was performed to demonstrate the capability of the participating National Metrology Institutes (NMIs) to measure the amount content of H<SUP>+</SUP>, ν<SUB>H<SUP>+</SUP></SUB>, in an HCl solution with a nominal ν<SUB>H<SUP>+</SUP></SUB> of 0.1 mol kg<SUP>−1</SUP>. The comparison was a joint activity of the Electrochemical Working Group (EAWG) and Inorganic Analysis Working Group (IAWG) of the CCQM and was coordinated by NIST (USA) and CENAM (Mexico).</P><P>The agreement of the results was not commensurate with the claimed uncertainties of the subset of participants that claimed small uncertainties for this determination. A workshop on technical issues relating to the CCQM-K73 measurements was conducted at the joint IAWG-EAWG meeting at the Bureau International des Poids et Mesures (BIPM), Paris (Sèvres) in April 2010. Several possible sources of bias were investigated, but none could explain the observed dispersion among the participants' results.</P><P>In the absence of a specific cause for the dispersion, the IAWG and EAWG decided to assign a Key Comparison Reference Value, KCRV, and standard uncertainty of the KCRV, u<SUB>KCRV</SUB>, based on the DerSimonian–Laird statistical estimator. The u<SUB>KCRV</SUB> is dominated by the between-laboratory scatter of results in CCQM-K73. The uncertainty estimates from the participants with the lowest reported uncertainties remain unsupported by this KC.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>
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