Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

重化學工業機械의 國産化方案에 關한 硏究 : 特히 窯業에 있어서의 燒成爐, 粉碎機, 排風機, 冷却機, 電氣集마器 自動枰量供給器 等의 製作을 目的으로

趙哲衡,朴碩喆,丁太權,宋鐵,桭達福,金基玉,朴煥奎,趙煥從,朴善鐘,金種一,李茂錫 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

This is to investigate the posibility of home manufacturing of heavy chemical industry machines, such as rotary kiln, crusher, blower, cooler, electrostatic precipitator and weighing feeder of cement plant. It is concluded that even though we can not make all of them (some of them are made now and some others are going to be made in the near future, some of them are made whole and some others are made partially), we can build or export the cement plant by importing the important machines which we can not make now and by substituting them with ours gradually.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

성문감정의 현황과 문제점

박종철,홍수기 大韓法醫學會 1992 대한법의학회지 Vol.16 No.2

We have used the "Voiceprint" technique to identify individuals from their speech in real cases for 5 years. This technique is one of the reasonably valid investigational tools. In this paper we report the present status of the speaker identification and discuss some of the problems encountered in real cases.

골격성 Ⅲ급 부정교합자에서 중심위 변위가 두부 방사선 계측치에 미치는 영향

박양수,김종철,황현식 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.3

본 연구는 Ⅲ급 부정교합자에서 중심교합상태의 두부 방사선 사진과 MPI 수치를 이용하여 중심위로 전환한 두부 방사선 사진(Centrically related cephalometrics)를 계측하여 중심위 변위시 유의한 계측치 변화가 있는지 확인하고자 시행되었다. 교정치료의 경험이 없는 25명의 Ⅲ급 부정교합군과 25명의 정상교합군을 대상으로 하여, 중심교합위 상태의 측모 두부 방사선 사진과 중심위 및 중심교합위 기록을 채득하고, 모형을 SAMⅡ 교합기에 부착하였다. 중심위 변위량은 MPI(Mandibular Position Indicator)를 이용하여 계측하였으며, MPI 수치와 중심교합위 상태의 측모 두부 방사선 사진의 투사도를 이용하여 Conversion work sheet상에서 중심위 전환을 시행하였다. 두부 상사선 계측 항목은 하악골의 위치와 관계되는 것을 선택하였다. 정상교합군과 Ⅲ급 부정교합군에서 중심위와 중심교합위간의 계측치 변화를 연구하여 다음과 같은 결과를 얻었다. 1. 하악 과두에서의 중심위 변위량은 정상교합군에서 전후방 변위량(ΔX)0.56±0.50mm, 수직적 변위량(ΔZ) -0.61±0.53mm였으며, Ⅲ급 부정교합군에서 전후방 변위량 (ΔX) 0.093±0.77mm, 수직적 변위량(ΔZ) -0.64±0.89mm로, 두군 보두 후하방 변위양상을 나타냈다. Ⅲ급 부정교합군에서 정상교합군보다 전후방 변위량이 더 크게 나타났으나 통계학적인 유의성은 없었다. 2. 중심교합위와 중심위 두부 방사선 사진의 계측치를 비교해 본 결과 정상 교합군에서 ANB, Facial angle, Facial convexity, ODI 등의 항목이 유의한 차이를 나타냈다. Ⅲ급 부정 교합군에서는 ANB, Facial angle, facial convexity, ODI, SNB, APDI, L1-FP 등 유의한 차이를 보이는 항목이 더 많았고 유의성도 더 높게 나타났다. 3. 두부 방사선 사진의 계측치 변화는 중심위 변위와는 연관성이 있었으나, 정상교합군과 Ⅲ급 부정교합군에 대한 연관성이 없었다. 이상의 결과는 중심의 변위량이 임상적으로 재현할 수 있는 중심위 위치에서 정상적으로 변위되는 양보다 큰 경우 중심위는 교정 환자에서 적절한 진단과 치료 계획 수립을 위한 기준 점으로 고려되어야 함을 시사하였다. The purpose of this study was to investigate if there were a significant difference between cephalometric measurements of mandibular position derived from a centric occlusion tracing compared to those of a converted centric relation tracing in the Class Ⅲ malocclusion. The sample consisted of 25 ClassⅢ malocclusion and 25 normal occlusion persons who had no orthodontic treatment. The records included an lateral cephalometrics in centric occlusion, centric relation and centric occlusion bite registration and diagnostic casts mounted on the SAM Ⅱ articulator in CR. The amount of CR-CO discrepancy of condyle was recorded using a MPI(Mandibular Position Indicator, MPI 200, Great Lakes Orthodontics, USA). The conversion of the CO cephalogram to CR using the MPI readings was performed on the Conversion work sheet. Measures of mandibular position were chosen for the purpose of this study. The comparison of the difference between CO and CR cephalometric measurements in the normal occlusion and Class Ⅲ malocclusion group were studied. The results were as follows: 1. In the features of CR-CO discrepancy of the condyle, the condyle was displaced posterior and inferior when the teeth were in centric occlusion. The horizontal component(ΔX) in Class Ⅲ malocclusion group was greater than the vertical component(ΔX) and also greater than the horizontal component(ΔZ) in normal occlusion group. There was no statistically significant correlation between MPI measurements and the groups of normal occlusion and Class Ⅲ malocclusion group. 2. In the comparison of the cephalometric measurements in each group, Normal occlusion group showed significant difference in measurements such as ANB, Facial angle, Facial convexity and ODI. ClassⅢ malocclusion group showed significant difference in measurements such as ANB, Facial angle, Facial convexity, OEI, SNB, APDI, L1-FP and it had more significance than the normal occlusion group. 3. The Value of cephalometric measurements was significantly different between CO and CR but there were no differences between the groups of normal occlusion and Class Ⅲ malocclusion. The results of this study suggest that if the discrepancies are greater than the amount of normal displacement from clinically captured centric relation, centric relation should be considered as the starting point for proper diagnosis and treatment planning.

고압하에서 cyclotetramine 착이온(Cu-N_4^2+)과 음이온(S_2O_3^2-, SCN^-, I^- 및 NO_2^-)간의 화학평형반응

박유철,변종철 慶北大學校 物理化學硏究所 1984 硏究論文集 Vol.5 No.-

The equilibrium reactions between dl-5, 5, 7, 12, 12, 14-hexamethyl-1, 4, 8, 11-tetraazacyclotetradeca-1, 7-dienecopper(Ⅱ) complex ion and the basic ligands, L, given below have been investigated in a aqueous solution by using spectrophotometric method. Cu(dl-trans-1, 7-diene)^2+ + L^n- ⇔ 〔Cu(dl-trans-1, 7-diene)·L〕^(2-n)+ In the reaction equation, L were S_2O_3^2-, SCN^-, I^- and NO_2^-. The absorption maximum of the complex ion, Cu(dl-trans-1, 7-diene)^2+ shifted to longer wave-lengths in the above ligand solutions. The pressure effects on the chemical equilibrium were measured in the range of 1-1500 bar. The measurements of pressure effect on the absorption spectrum showed an decrease in an extinction coefficient with increasing pressure. At the room temperature, the equilibrium constants decreased with increasing pressure for anionic ligands, S_2O_3^2-, SCN^-, I^- and NO_2^- were 10.7, 2.4, 1.8 and 1.2 at 1 bar and 3.0, 1.0, 0.3 and 0.5 at 1,500 bar, respectively. The changes of reaction volume were the positives which are agreed with the trend of the reaction entropy changes. The pressures, at which the changes of reaction volume were no more appeared, were around 500 bar for SCN^- and NO_2^- ions, while were around 1,500 bar for S_2O_3^2- and I^- ions. By comparing both the equilibrium constants experimentally determined and those calculated according to Fuoss's ion-pair equation, the deriving forces of the adduct formed, [Cu(dl-trans-1, 7-diene)·L〕^(2-n)+ were discussed.