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Inhibition of Microbial Quorum Sensing Mediated Virulence Factors by Pestalotiopsis sydowiana
Paramanantham Parasuraman,B Devadatha,V. Venkateswara Sarma,Sampathkumar Ranganathan,Dinakara Rao Ampasala,Dhanasekhar Reddy,Ranjith Kumavath,김인원,Sanjay K. S. Patel,Vipin Chandra Kalia,이정걸,Busi Siddha 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4
Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal (Pestalotiopsis sydowiana PPR) extract was found to possess effective antipathogenic characteristics. The minimum inhibitory concentration (MIC) of the fungal extract against test pathogen Pseudomonas aeruginosa PAO1 was 1,000 μg/ml. Sub-MIC concentrations (250 and 500 μg/ml) of fungal extract reduced QSregulated virulence phenotypes such as the production of pyocyanin, chitinase, protease, elastase, and staphylolytic activity in P. aeruginosa PAO1 by 84.15%, 73.15%, 67.37%, 62.37%, and 33.65%, respectively. Moreover, it also reduced the production of exopolysaccharides (74.99%), rhamnolipids (68.01%), and alginate (54.98%), and inhibited the biofilm formation of the bacteria by 90.54%. In silico analysis revealed that the metabolite of P. sydowiana PPR binds to the bacterial QS receptor proteins (LasR and RhlR) similar to their respective natural signaling molecules. Cyclo(- Leu-Pro) (CLP) and 4-Hydroxyphenylacetamide (4-HPA) were identified as potent bioactive compounds among the metabolites of P. sydowiana PPR using in silico approaches. The MIC values of CLP and 4-HPA against P. aeruginosa PAO1 were determined as 250 and 125 μg/ml, respectively. All the antivirulence assays were conducted at sub-MIC concentrations of CLP (125 μg/ml) and 4-HPA (62.5 μg/ml), which resulted in marked reduction in all the investigated virulence factors. This was further supported by gene expression studies. The findings suggest that the metabolites of P. sydowiana PPR can be employed as promising QS inhibitors that target pathogenic bacteria.
S Paramanantham, SalaiSargunan,Ha, Cong-Tu,Park, Warn-Gyu Elsevier 2018 International journal of mechanical sciences Vol.136 No.-
<P><B>Abstract</B></P> <P>Numerical investigations of the condensing behaviors of a single bubble and multiple bubbles under different subcooled flows were conducted using the compressible homogeneous mixture method. The surface tension between the liquid and vapor phases was determined using the continuous surface force method. An implicit dual-time technique was applied to solve the two-phase compressible flow model. A sensitivity study of the empirical coefficients used to access the predictive capacity of the existing mass transfer models was conducted. The numerical simulation results were compared with experimental data. The obtained results agreed well with the experimental results. Additionally, the bubble condensation behavior was investigated for different initial subcooled temperatures, bubble diameters, and velocities. Subsequently, the effect of multiple bubble interactions on their condensation was studied. The condensation rate of the lower bubbles increased compared to the other bubbles as a result of induced random perturbation. When the gap between the bubbles was large, a negligible amount of condensation occurred between the bubbles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fully compressible two-phase homogenous mixture model for single and multiple bubble condensation is presented. </LI> <LI> Results were validated with experiment data under different flow conditions. </LI> <LI> The effects of initial bubble size, subcooled temperature, and velocity on condensation are investigated. </LI> <LI> The bubble–bubble condensation rate of the lower bubbles increased as a result of the random agitation caused by other bubbles. </LI> </UL> </P>
Scutellarein Induced G2/M Cell Cycle Arrest and Apoptosis in Hep3B Hepatocellular Carcinoma Cells
Sang Eun Ha,Ho Jeong Lee,Suchismita Raha,Venu Venkatarame Gowda Saralamma,Seong Min Kim,Anjugam Paramanantham,Jeong Doo Heo,Sang Joon Lee,Eun Hee Kim,Gon Sup Kim 대한수의학회 2017 대한수의학회 학술대회발표집 Vol.2017 No.-