물고기 배자를 이용한 acetaminophen의 내분비교란성 탐색

김판기 용인대학교 자연과학연구소 2011 自然科學硏究所論文誌 Vol.16 No.1

Acetaminophen (AA) is a commonly used analgesic and antipyretic drug that is present in >850 over-the-counter and prescription formulas. AA is taken acutely, as well as chronically, by millions of adults and children. Metabolism of AA, primarily in the liver by mixed function oxidases, can result in the production of a highly reactive metabolite, N-acetyl-p-benzoquinoneimine (NAPQI). At high doses acetaminophen elicits well-characterized hepatotoxic effects due to the production of NAPQI, which are exacerbated by ethanol consumption. Several in vivo studies suggest AA may alter some hormone-regulated processes in reproductive tissues. AA reduced the reproductive capacity, testicular weight, and spermatogenesis of mice and reduced E2-induced uterine peroxidase activity and nuclear progesterone receptor protein in immature mice. In humans, AA had no significant effect on breast cancer risk but reduced the risk of ovarian cancer and significantly lowered basal levels of gonadotropin and estradiol. However, AA did not increase uterine wet weight in mice or rats, and did not affect E2-induced increases in rodent uterus weight. In this study, toxicogenomics used to find out the outcome of this modulating in fish embryo. The results could not found out the exact findings of this effects, but you can use this protocols using fish embryo and some markers in this purpose.

솔잎 및 감 추출물의 항돌연변이원성

김판기,권은아,석지현,박창은 龍仁大學校 自然科學硏究所 1999 自然科學硏究所論文誌 Vol.4 No.2

The tannic acid fraction dele extracted from pine leaf and the skin of tile persimmon cultivated in Korea on 1998. To evaluate antimutagenicity of the water soluble tannic acid fraction, we used the Ames test which is a well known test method of the world. In the reverse mutation test did induced antimutagenicity in Salmonella typhimurium TA98, TA100, TA155, TA1537 with and without metabolic activation. Tannic acid fraction(0.1㎖/plate) were inoculated in the test tube which were spreaded the Salmonella spp. After the pre-incubatior for 37℃ , 15 minutes, positive control added 0.1 ㎖. In the TA98, negative control reverse mutation colony 14.6 at S9 negative, 19.9 at S9 Positive. Also positive control reverse mutation colony 1,356 at S9 negative, 1,175 at S9 positive. But, in the pine leaf extracts additive groups were: recovered to the extents of negative control group, such as 130-605 reverse mutation colony. In the persimmon skin extracts group, positive contro1 groups were 1,261, at S9 negative group, 1,524 at S9 positive group of reverse mutation colony. But, in the persimmon skin extracts added groups were decreased to the extents of negative contro1 group such as 185-484 reverse mutation colony. Such like a manner, pine leaf extract and persimmon skin extracts were decreased the reverse mutation colony counts induced by sodium azide 2-aminoanthracene, 9-aminoacridine, 2-aminofluorene. It was concluded that water soluble tannic acid fraction from pine leaf and skin of the persimmons did induced antimutagenic effects of the known mutagenic insult.

정제 목초액의 아급성 독성시험

김판기 용인대학교 자연과학연구소 1996 自然科學硏究所論文誌 Vol.1 No.1

Refined wood vinegar is one of the polyacidic solution of which main component is acetic acid. We investigated the subchronic toxicity of the refined wood vinegar using SPF ICR mouse for 4 weeks. The refined wood vinegar was administered by gastic intubation, 0.5, 1.0, 2.5, 5.0 g/kg body weight. The results were as follows : 1. There are no adverse effects on the clinical obserbation and body weight changes. Also, there are some significant changes in organ weight, but it was meaningless because of the absence of dose-response relationships. 2. In the hematological patterns of administered mouse, there are no significant changes between the treated groups. Also, there are no serological enzymatic changes in the treated mouse. In the 1.0 g/kg treated group, ASP activity was increased significantly compared with control group. But, this level of activity was fall under the normal physiological range of control mouse. 3. Histopathological findings of the brain, liver, heart, spleen, kidneys, stomach, lung, testis, ovary, uterus and thymus were not observed in the treated mouse. From the above results, the refined wood vinegar has no toxicity upto the 5.0 g/kg/day of oral dose for 4 weeks.

카드뮴과 에탄올이 姙娠 랫드에 미치는 影響에 관한 硏究

김판기 龍仁大學校 1997 용인대학교 논문집 Vol.14 No.-

The teratogenic effects of cadmium and ethanol on pregnant Wistar rats were examined. The results of the study were as follows : Cadmium treated (16μmole/kg) rat on gestational day 9-11, had 10 fetuses on male and female ratio 5:5. The fetuses had tail hematoma only 4 cases and the dams had late death in 1 cases. But all fetuses didn't have any type of abnormality in treated dams. Ethanol treated(4.8g/kg) rat on gestational day 7, had 9 fetuses on male and female ratio 6:3. The fetuses didn't have any type of teratogenic fetus but the pregnacy ratio was only 40.9%. The exposure of ethanol on the day of early gestational period showed high postimplantaion loss on rat.

교내 일부 실내환경위생 기초조사

김판기 용인대학교 자연과학연구소 2002 自然科學硏究所論文誌 Vol.7 No.1

This study was carried out to determine the adequacy of indoor environments in school cafeteria, lecture room, corridor, library and student activity room. In this study, carbon monooxide concentration, oxigen content, illumination intensity and noise were included. For this study, three places of lecture room in Natural Science Building, 3 reading room of library, the cafeteria at Natural Science Building, an practice room at Natural Science Building were surveyed and measured two times from each places during November. The following results were obtained; The carbonmonooxide concentrations were increased to the 30ppm at restroom and student study room, due to the tobacco smoking. The oxygen contents was 21.3 - 21.9%. The illuminations were varied to the places, the passage was low to the 43 - 145Lux. More illuminations were needed at passages and laboratory room. The noise were varied at the sites, the high noise was recorded at the passage 90.1dB. The noise of passages were a hazard factor to establish academic pursuit. The hygienic indoor environment of the campus were varied, but it is important for academic pursuit to do regular management in illuminations and control policy of noise at campus.

카드뮴의 조직내 축적에 미치는 솔잎, 감피, 율피에서 추출한 탄닌산의 영향

김판기 龍仁大學校 自然科學硏究所 1999 自然科學硏究所論文誌 Vol.4 No.2

The tannic acid (1.Omg/ml) and/ or cadmium (30mg/kg) were administered by oral administration. The results were as follows : 1. There were adverse effects on the weight changes and water consumption. But, the extent of adverse changes were not changed by tannic acid administration significantly. 2.In the hematological patterns of administered mice, there were: significant changes between cadmium treated groups and control group. Hemoglobin contents, packed cell volume, platelet count and neutrophill count were changed significantly compared with control group. These changes were not changed in tannic acid treated group significantly. 3. Cadmium concentrations varied in the cadmium treated mouse ]iver, kidndy, spleen and testes. In the tannic acid treated groups pine leafs, persimmon skin and chestnut skin extracts cadmium concentrations of liver and kidneys were decreased to the extent of an half of the cadmium only treated group's From the above results, the tannic acid extracted from pine leafs, persimmon skin and chestnut skin had alleviative effects of cadmium accumulation upto the 1.0 mg/ml/day of oral dose for 3 weeks.

4-chlorophenol 이 daphnia의 급성독성에 미치는 영향

김판기,최성부 용인대학교 자연과학연구소 2003 自然科學硏究所論文誌 Vol.8 No.1

Laboratory studies have suggested that some alkylphenol and pesticides ehcit developmental toxcity to crustaceans. The purpose of the present study was to evaluate the possibility that the alkylphenol degradation product 4-chlorophenol is acute toxicity to the crustacean Daphnia magna. Arthropod (insects, crustaceans, and several minor phyla) endocrinology is dominated by the involvement of terpenoid, ecdysteroid, and peptide hormone Some evidence suggests a role for vertebrate-type sex steroids (androgens, estrogens, progestogens) in regulating various reproductive processes in crustaceans. The paucity of the data argues that the observed effects of these steroids reflect the disruption of endocrine pathways that nomally use other hormones rather than the stimulation of path way that are specifically responsive to the adminstered hormone We had experiments for 5 times of 4-chlorophenol LC_50 finding study, that was 38.7μM of this chemical This value compares favorably with previously reported values based on daphid toxicity. They do substantiate the need to evluate the extent to which other crustacean species are susceptible to this mode of toxicity and to estabhsh the susceptibihty of other crustaceans relative to daphnids

환경호르몬(내분비장애물질)과 식품안전

김판기 龍仁大學校 自然科學硏究所 1999 自然科學硏究所論文誌 Vol.4 No.1

During the past 50 or more years, vast quantities of diverse synthetic chemicals (xenobiotics) have entered the environment because of efforts to increase agricultural productivity and because of modern industrial processes. These chemicals include herbicides, insecticides, fungicides, styrenes, polychlorinated biphenyls, and penta- to nonylphenols. Some xenobiotics have been shown to disrupt normal endocrine functions, which leads to aberrant development of female and male reproductive tissues and results in decreased fertility or sterility. The regulation of synthetic chemicals for endocrine disrupting properties may not alleviate the observed increases in human bresat and testicular cancer or the apparent decrease in human sperm counts and sperm quality reported for some countries. Therefore, while attending to one possible contributor to these problems - synthetic chemicals - we should remain alter to the possible importance of alternative contributory factors, such as diet and lifestyle. Evaluation of the health and environmental effects of endocrine disruptors will be most credible when infromation is available at several levels such as toxicity, student, and field studies. Well-planned and coordinated mutidisciplinary studies are encouraged.

카드뮴이 랫드에 미치는 독성학적 변화에 관한 연구

김판기 龍仁大學校 1997 용인대학교 논문집 Vol.13 No.-

The acute and chronic effects of cadmium(Cd, CdC12 20mg/kg) on Wistar male rats were evaluated concerning cadmium contents, histopathological examinations. The results of the study were as follows : 1. Less cadmium was absorbed through the digestive tracts, but the ratio of contents in renal to hepatic cadmium was higher at 8 weeks after treatment. 2. Histopathological changes in cadmium treated rats were appeared at 8 weeks after treatment in kidneys. Homogenous eosinophilic material was accumulated in cortical and collecting tubular lumens at 16 weeks. Degenerated or necrotized tubular cells were observed in cortex and medulla. Degenerated seminiferous tubules and homogeneous eosinophilic material was seen in interstitial tissue of rat treated with cadmium for 16 weeks. Calcium deposits were seen in degenerated seminiferous tubules and the tubules showed severe calcification of rat treated with cadmium for 16weeks. From the above results, it could be concluded that cadmium was toxic to the kidney and testis especially in chronic treatment.

인체 프탈레이트 노출평가 : 원리 및 방법

이장우,김판기 용인대학교 자연과학연구소 2008 自然科學硏究所論文誌 Vol.13 No.1

Phthalates are used as plasticizers in PVC (polyvinyl chloride) plastics. As the phthalate plasticizers are not chemically bound to PVC, they can leach, migrate or evaporate into indoor air and atmosphere, foodstuff, other materials, etc. Consumer products containing phthalates can result in human exposure through direct contact and use, indirectly through leaching into other products, or general environmental contamination. Humans are exposed through ingestion, inhalation, and dermal exposure during their whole lifetime, including intrauterine development. And phthalate esters have adverse effects on liver, kidney, and the reproductive system, and act as weak endocrine disrupting agents. Expecially, university students have many opportunities to come in contact with the products included phthalate during the campus life (food packaging, body care product, cosmetic, lotions, aftershave, perfume etc). The purpose of this study was to examine the principles and methods of phthalate exposure. Because everyone used to PCPP (pharmaceuticals and personal care products) including phthalates and do smoking or drinking or eating.