Clinicopathological Features and Survival of Young Turkish Patients with Testicular Germ Cell Tumors

Ozgun, Alpaslan,Karagoz, Bulent,Tuncel, Tolga,Emirzeoglu, Levent,Celik, Serkan,Bilgi, Oguz Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Testicular germ cell tumors (TGCTs) are a relatively common malignancy in young men. The aim of this study was to investigate the clinicopathological features and survival of young Turkish patients with TGCT. Materials and Methods: In this retrospective study, the clinical and pathological characteristics of young Turkish patients with TGCT who were monitored by the Department of Medical Oncology of a military hospital between 2008 and 2013 were investigated. Overall survival data were analyzed. Results: Ninety-six patients were included in the study. The mean age was 26.4 years. Among the patients, 17.7% had seminoma and 43.8% had mixed non-seminomatous germ cell tumors. Some 46.9% were Stage I, 30.2% were Stage II, and 22.9 were Stage III. Of the patients, 83.3% received chemotherapy, 25% underwent retroperitoneal lymph node dissection (RPLND), 3.1% received radiotherapy, and 12.5% were followed-up without treatment. In addition, 18.8% of the patients were administered salvage chemotherapy due to relapse or progression. The 5-year overall survival rate was 90.2% for all patients. The 2-year overall survival rate was 100% for Stage I patients, 94% for Stage II patients, and 70.2% for Stage III patients. The difference between the survival curves of stages was statistically significant (p=0.029). Conclusions: In young Turkish patients with TGCT, good results were obtained with appropriate treatment, most receiving chemotherapy. The prognosis of the disease was good even in the advanced stage.

Correlation of Post Swim-Up Acrosome Index with in-vitro Fertilization Outcomes

Meltem Ozguner,Oya Evirgen,Baha Oral 연세대학교의과대학 2009 Yonsei medical journal Vol.50 No.3

Purpose: A prospective study was planned to determine the relationship between post swim-up acrosome index (AI) evaluation and fertilization outcomes in an in vitro fertilization (IVF) program. Materials and Methods: Infertile couples who have applied to IVF were admitted into this study when the male partner’s sperm concentration was > 20×106/mL and motility > 30%. Pre- and post swim-up semen quality parameters including concentration, motility, sperm morphology and AI were evaluated in a prospective, randomized and blinded fashion. The couples were divided prospectively into 2 groups. In group I (25 couples) 50 000 sperm per oocyte were used for insemination considering post swim-up acrosome index, and in group II (25 couples) 50 000 sperm per oocyte were used for insemination without considering post swim-up acrosome index. Results: Pre- and post swim-up AI were 30.8 ± 3.4 and 17.8 ± 4.5 in group I, and 31.4 ± 3.6 and 16.3 ± 4.7 in group II (p > 0.05) respectively. The significant improvement in morphology and motility after double wash swim-up procedure has been observed. However, double wash swim-up procedure could not eliminate head and especially acrosomal defects which would directly effect fertilization capacity in conventional IVF program. In group I, 85.3% of oocytes were fertilized, with a 48% pregnancy rate; in group II, 71.0% of oocytes were fertilized, with a pregnancy rate of 20%. Fertilization and pregnancy rates were significantly different (p < 0.05) between the two groups. Conclusion: We have concluded that it could be useful to consider post swim-up AI of sperm inseminated in conventional IVF cycles, which correlates with high fertilization and pregnancy rates. Purpose: A prospective study was planned to determine the relationship between post swim-up acrosome index (AI) evaluation and fertilization outcomes in an in vitro fertilization (IVF) program. Materials and Methods: Infertile couples who have applied to IVF were admitted into this study when the male partner’s sperm concentration was > 20×106/mL and motility > 30%. Pre- and post swim-up semen quality parameters including concentration, motility, sperm morphology and AI were evaluated in a prospective, randomized and blinded fashion. The couples were divided prospectively into 2 groups. In group I (25 couples) 50 000 sperm per oocyte were used for insemination considering post swim-up acrosome index, and in group II (25 couples) 50 000 sperm per oocyte were used for insemination without considering post swim-up acrosome index. Results: Pre- and post swim-up AI were 30.8 ± 3.4 and 17.8 ± 4.5 in group I, and 31.4 ± 3.6 and 16.3 ± 4.7 in group II (p > 0.05) respectively. The significant improvement in morphology and motility after double wash swim-up procedure has been observed. However, double wash swim-up procedure could not eliminate head and especially acrosomal defects which would directly effect fertilization capacity in conventional IVF program. In group I, 85.3% of oocytes were fertilized, with a 48% pregnancy rate; in group II, 71.0% of oocytes were fertilized, with a pregnancy rate of 20%. Fertilization and pregnancy rates were significantly different (p < 0.05) between the two groups. Conclusion: We have concluded that it could be useful to consider post swim-up AI of sperm inseminated in conventional IVF cycles, which correlates with high fertilization and pregnancy rates.

Synthesis, characterization, and antimicrobial activities of 3-HPAA-Alg-Chi nanoparticles

Ozdemir, Ozgun O.,Soyer, Ferda Techno-Press 2021 Advances in nano research Vol.11 No.3

Encapsulation of bioactive compounds (e.g., phenolic acids) into nanoparticles is a well-received technique in the searching for new antimicrobial agents against multidrug-resistant pathogens. Encapsulation can be a good technique to maintain the stability of phenolic acids against environmental conditions. In this study, 3-hydroxyphenylacetic acid (3-HPAA) was encapsulated into alginate-chitosan nanoparticles with the ion gelation technique. The characterization of loaded and unloaded nanoparticles was performed via dynamic light scattering, Fourier transform infrared spectroscopy, and scanning electron microscopy. According to the results, 3-HPAA loaded nanoparticles have spherical shapes with a diameter range of 40-80 nm and an average hydrodynamic diameter of 361.0 ± 69.8 nm. The loading of 3-HPAA was successfully achieved based on the Fourier transform infrared spectra and encapsulation percentage studies. The antimicrobial effect of the nanoparticles in solution forms was tested on P. aeruginosa, S. epidermidis, MRSA, and MSSA. The results demonstrated that the 3-HPAA loaded alginate chitosan nanoparticle solution showed elevated antimicrobial effect due to the pH change by treatment with 1% acetic acid, and it displayed bacteriocidal effects in a strain-specific and dose-dependent manner. Therefore, the 3-HPAA loaded alginate chitosan nanoparticle solution was produced successfully with the bacteriocidal effect against serious pathogenic bacteria.

Effect of fermented tuna by-product containing diets with and without Song-gang stone<SUP>®</SUP> on growth, immune response and disease resistance in juvenile olive flounder Paralichthys olivaceus

Fatma Ozgun Oncul,Frolan A. Aya,Minji Seong,Jin Hyeok Lee,Sungchul C. Bai 한국수산과학회 양식분과 2017 한국수산과학회 양식분과 학술대회 Vol.2017 No.5

Effects of Tumor Necrosis Factor Alpha Blocker Adalimumab in Experimental Spinal Cord Injury

Borcek, Alp Ozgun,Civi, Soner,Ocal, Ozgur,Gulbahar, Ozlem The Korean Neurosurgical Society 2015 Journal of Korean neurosurgical society Vol.57 No.2

Objective : Tumor necrosis factor alpha (TNF-${\alpha}$) have proven effects in pathogenesis of neuroinflammation after spinal cord injury (SCI). Current study is designed to evaluate the effects of an anti-TNF-${\alpha}$ agent, adalimumab, on spinal cord clip compression injury in rats. Methods : Thirty two male adult Wistar rats were divided into four groups (sham, trauma, infliximab, and adalimumab groups) and SCI was introduced using an aneurysm clip. Animals in treatment groups received 5 mg/kg subcutaneous adalimumab and infliximab right after the trauma. Malondialdehyde (MDA) levels were studied in traumatized spinal cord tissues 72 hours after the injury as a marker of lipid peroxidation. Results : Animals that received anti-TNF-${\alpha}$ agents are found to have significantly decreased MDA levels. MDA levels were significantly different between the trauma and infliximab groups (p<0.01) and trauma and adalimumab groups (p=0.022). There was no significant difference in neurological evaluation of the rats using Tarlov scale. Conclusion : These results suggest that, like infliximab, adalimumab has favorable effects on lipid peroxidation induced by spinal cord trauma in rats.

Mean Platelet Volume as a Prognostic Marker in Metastatic Colorectal Cancer Patients Treated with Bevacizumab-Combined Chemotherapy

Tunce, Tolga,Ozgun, Alpaslan,Emirzeoglu, Levent,Celik, Serkan,Bilgi, Oguz,Karagoz, Bulent Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Recent studies have revealed a prognostic impact of the MPV (mean platelet volume)/platelet count ratio in terms of survival in advanced non-small cell lung cancer. However, there has been no direct analysis of the survival impact of MPV in patients with mCRC. The aim of the study is to evaluate the pretreatment MPV of patients with metastatic and non-metastatic colorectal cancer (non-mCRC) and also the prognostic significance of pretreatment MPV to progression in mCRC patients treated with bevacizumab-combined chemotherapy. Materials and Methods: Fifty-three metastatic and ninety-five non-metastatic colorectal cancer patients were included into the study. Data on sex, age, lymph node status, MPV, platelet and platecrit (PCT) levels were obtained retrospectively from the patient medical records. Results: The MPV was significantly higher in the patients with mCRC compared to those with non-mCRC ($7.895{\pm}1.060$ versus $7.322{\pm}1.136$, p=0.013). The benefit of bevacizumab on PFS was significantly greater among the patients with low MPV than those with high MPV. The hazard ratio (HR) of disease progression was 0.41 (95%CI, 0.174-0.986; p=0.04). In conclusion, despite the retrospective design and small sample size, MPV can be considered a prognostic factor for mCRC patients treated with bevacizumab-combined chemotherapy.

미래도시의 교통신호 제어 데이터 수집에 관한 연구

서강도,Umit Ozguner,조진호 한국ITS학회 2007 한국ITS학회 학술대회 Vol.2007 No.1

Immunoregulatory Function of HLA-G in Gastric Cancer

Tuncel, Tolga,Karagoz, Bulent,Haholu, Aptullah,Ozgun, Alpaslan,Emirzeoglu, Levent,Bilgi, Oguz,Kandemir, Emin Gokhan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Human leukocyte antigen (HLA)-G-positive gastric cancers are associated with poor survival, but links with tumor escape mechanisms remain to be determined. Materials and Methods: We used immunohistochemistry to investigate HLA-G expression, tumor infiltrating CD8+ T lymphocytes, and Treg cells in 52 gastric cancer patients. Results: There were 29 cancer-related deaths during the follow-up period. Kaplan-Meier analysis indicated that patients with HLA-G-positive (n=16) primary tumors had a significantly poorer prognosis than patients with HLA-G-negative tumors (n=36, p=0.008). The median survival time was 14 months and 47 months, respectively. Patients with high numbers of Tregs and low numbers of CD8+T lymphocytes in the primary tumor had a poorer prognosis than those with low numbers of Tregs and high numbers of CD8+T lymphocytes (p=0.034, p=0.043). Multivariate Cox proportional hazard regression analysis showed that HLA-G expression (hazard ratio: 2.662; 95% confidence interval: 1.242-5.723; p=0.012) and stage (hazard ratio: 2.012;95% confidence interval: 1.112-3.715; p=0.041) were independent unfavorable factors for patient survival. Conclusions: We found a significant positive correlation between HLA-G expression and the number of tumor infiltrating Tregs (p=0.01) and a negative correlation with the number of CD8+T lymphocytes (p=0.041). HLA-G may protect gastric cancer cells from cytolysis by inducing Foxp3+Treg lymphocytes and suppressing CD8+T lymphocytes.