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Phenolic composition, enzyme inhibitory, and antioxidant activity of Bituminaria bituminosa
Sarikurkcu, Cengiz,Cengiz, Mustafa,Uren, Mehmet Cemil,Ceylan, Olcay,Orenc, Tuba,Tepe, Bektas 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
This study aimed to evaluate the in vitro antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of Bituminaria bituminosa. In phosphomolybdenum assay, the methanol extract showed the highest activity ($166.78{\mu}mol\;TEs/g$ dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl ($DPPH^{\bullet}$) and 2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid) ($ABTS^{{\bullet}+}$). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and $46.82{\mu}mol\;TEs/g$ dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, ${\alpha}$-glucosidase inhibition assay resulted in the superiority of water extract ($1233.86{\mu}mol\;ACEs/g$ dry plant). In the case of ${\alpha}$-amylase inhibitory assay, the ethyl acetate extract showed the highest activity ($53.65{\mu}mol\;ACEs/g$ dry plant). The water extract exhibited the highest phenolic content ($31.70{\mu}mol\;GAEs/g$ dry plant). In contrast, the methanol extract was found rich in flavonoid compounds ($5.29{\mu}mol\;REs/g$ dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.
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Phenolic composition, enzyme inhibitory, and antioxidant activity of Bituminaria bituminosa
Cengiz Sarikurkcu,Mustafa Cengiz,Mehmet Cemil Uren,Olcay Ceylan,Tuba Orenc,Bektas Tepe 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
This study aimed to evaluate the in vitro antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of Bituminaria bituminosa. In phosphomolybdenum assay, the methanol extract showed the highest activity (166.78 μmol TEs/g dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2-azino-bis (3- ethylbenzothiazloine-6-sulphonic acid) (ABTS•+). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and 46.82 μmol TEs/g dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, α-glucosidase inhibition assay resulted in the superiority of water extract (1233.86 μmol ACEs/g dry plant). In the case of α-amylase inhibitory assay, the ethyl acetate extract showed the highest activity (53.65 μmol ACEs/g dry plant). The water extract exhibited the highest phenolic content (31.70 μmol GAEs/g dry plant). In contrast, the methanol extract was found rich in flavonoid compounds (5.29 μmol REs/g dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.
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