Differences in Cotyledon Color and Harvest Period Affect the Contents of Major Isoflavones and Anthocyanins in Black Soybeans

( Yu-mi Choi ),( Hyemyeong Yoon ),( Myoung-jae Shin ),( Yoonjung Lee ),( Sukyeung Lee ),( On Sook Hur ),( Na Young Ro ),( Ho-cheol Ko ),( Jeongyoon Yi ),( Sang Hoon Lee ),( Heon-woong Kim ),( Yu Jin H 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.1

Several environmental and genetic factors affect the isoflavone and anthocyanin contents in soybeans. This study aimed to assess the influences of cotyledon color and harvest period on the contents of five major isoflavones and three major anthocyanins in 323 black soybean landraces grown in Korea. In all the soybeans, malonylgenistin, malonyldaidzin and cyanidin-3-O-glucoside were the foremost components. The total isoflavone (TIC) and total anthocyanin (TAC) contents were in the ranges of 491.29-1998.39 μg/g and 452.60-2789.37 mg/100 g on dry weight basis, respectively. Both the average TIC and TAC were higher in green cotyledon soybeans (1493.93 μg/g and 1656.05 mg/100 g, respectively) than in yellow cotyledon soybeans (1423.09 μg/g and 1489.30 mg/100 g, respectively). With respect to the harvest period, the average TIC was in the order of Late-October (1517.31 μg/g) > Mid-October (1454.54 μg/g) > Early-October (1340.95 μg/g). Moreover, the average TAC decreased in the order of Late-October (1765.76 mg/100 g) > Mid-October (1503.93 mg/100 g) > Early-October (1350.91 mg/100 g). In general, cotyledon color appeared to cause a significant variation on TAC (P < 0.05) but not on TIC, whereas the harvest period appeared to cause significant variations on both the TAC and TIC. Among the 323 landraces, 20 were identified to contain high TIC ( > 1800 μg/g) and TAC (> 2000 mg/100 g) in their seeds and hence, could be considered as important sources of dietary isoflavones and anthocyanins. Besides, they possibly provide a wide spectrum of options if considered during the development of improved soybean genotypes.

게임 유저 유형에 따른 SCI 수준 및 게임 몰입도에 관한 연구

온유연 ( On Yu Yeon ),조동민 ( Cho Dong Min ) 한국기초조형학회 2018 기초조형학연구 Vol.19 No.2

본 연구의 목적은 향후 캐릭터를 연구 및 개발 시 효과적으로 활용할 수 있는 실증적인 자료를 제공하고자 게임 유저의 유형에 따른 SCI(Self Character Identification) 수준과 게임 몰입도에 미치는 중요한 관계를 규명하는 것이다. 온라인 게임 이용률이 가장 높은 10대와 20대 게임 유저를 연구 대상으로 선정하였다. 독립변수인 게임 유저의 유형과 종속변수인 SCI와 게임 몰입도의 평균 차이를 비교하기 위해서 One-way ANOVA 분석을 실시하였고 게임 유저 유형별과 SCI(고/저)가 게임 몰입도에 주는 영향을 검증하고자 Two-way ANOVA 분석을 실시하였다. 연구 결과, 성별에 따른 게임 유저 유형은 유의미한 차이가 나타나지 않은 반면, 연령별 게임 유저의 유형은 연령대가 낮을수록 성취가형의 비중이 높아진 반면 연령대가 높을수록 살인자형의 비중이 높아졌다. 이를 통합하여 유형을 분류한 결과 살인자형이 가장 높은 비중을 차지하였고, 다음으로 성취가형, 사교가형, 모험가형 순으로 비중을 차지하였다. 유형별 SCI은 사교가형이 가장 높은 수준으로 나타났고, 모험가형은 다른 유형에 비해 상대적으로 낮은 수준으로 나타났다. 유형별 게임 몰입도는 사교가형이 가장 높은 수준으로 나타났고, 살인자형이 가장 낮은 수준으로 나타났다. 이는 사교가형의 특성인 게임 플레이 중 유저들 간의 관계 속 소통과 공감을 바탕으로 이뤄지는 감정 교감, 사회적 상호작용이 SCI와 게임 몰입도를 높이는 중요한 요인임을 알 수 있다. 또한 게임 유저 유형은 게임 몰입도에 영향을 미치나 SCI 수준은 게임 몰입도에 영향을 미치지 않는 것으로 나타났다. 이는 게임 몰입도를 높이기 위해서는 게임 유저가 추구하는 방향성과 성향, 각 유형별 특성을 파악하고 분석하여 게임 캐릭터를 개발해야 함을 의미한다. SCI와 게임 몰입도를 높이기 위해서는 각 유형별 고유한 특성은 지키되 사교가형의 특성을 결합하여 캐릭터를 개발해야 한다. The purpose of this study is to investigate the relationship between SCI (Self Character Identification) level and game flow according to the types of game users to provide empirical data that can be effectively utilized in future character research and development. The game users in their 10s and 20s, which is the highest in online game use rate, were selected. A one-way ANOVA was conducted to compare the mean difference between the type of game user, independent variable, SCI, the dependent variable, and the game flow. Two-way ANOVA was conducted to verify the effect of game user type and SCI (high/low) on game flow. As a result, there was no significant difference in game user types according to gender, while the type of game users by age showed a higher proportion of achievers type with the lower age group, but the higher the ages, the higher killers type. Based on this, the classification by type showed that killers type accounted the highest proportion, followed by achievers type, socializers type, and explorers type. The SCI by type showed the highest level in the socializers type and the level of explorers type was relatively low compared to other types. In the game flow by type, the socializers type showed the highest level and the killers type showed the lowest level. It indicates that emotional sympathy and social interaction based on communication and empathy in the relationship between users during game play, which is a characteristic of socializers type, are important factors for increasing SCI and game flow. Also, it showed that game user type influenced game flow, but SCI level did not affect game flow. This implies that in order to increase the game flow, game characters should be developed by analyzing the goals, tendencies, and characteristics of each type pursued by the game user. In order to increase the SCI and game flow, characters should be developed by combining characteristics of socializers type while maintaining characteristics of each type.

시스템 해석에 기초한 하수관망 오염 매카니즘과 관망 모니터링 및 이상진단

강온유 ( On Yu Kang ),이승철 ( Seung Chul Lee ),김민정 ( Min Jeong Kim ),유수민 ( Su Min Yu ),유창규 ( Chang Kyoo Yoo ) 한국화학공학회 2012 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.50 No.6

비점오염원은 세정에 의해 하수관 내로 유입되어 수계까지 흘러 수계의 오염부하를 가중시킬 뿐만 아니라, 관의 상 태에 따라 누수 및 월류를 야기하여 오염을 일으킨다. 이에 따라 하수관거의 유지관리 및 환경오염 방지를 위한 관거 내 하수의 유량, 수질, 불명수 및 월류 등을 효율적으로 관리하기 위한 모니터링의 필요성이 증가하고 있다. 그러나 하 수관거의 경우, 지하에 설계되며 그 구조 및 연결이 복잡한 특성으로 인해 실제 하수관거에 대한 모니터링은 쉽지 않 다. 본 연구에서는 시스템해석이론에 기초한 하수관망 오염제거 해석과 관망 모니터링 및 이상진단방법을 제시하였다. 먼저 하수관망 공정모사 프로그램인 Stormwater & Wastewater Management Model for expert (XP-SWMM)을 이용하 여 관망 내의 오염물질의 거동패턴을 해석하였다. 둘째, 다변량 통계 모니터링을 이용하여 하수관망 내의 수질 모니터 링 및 하수관망 유출을 탐지하는 관망 이상 진단을 수행하였다. 정적/동적 상태 시스템에 기초한 하수관망 오염 매카 니즘 해석결과, 강우시 총질소와 총인 부하량이 비강우시보다 급격하게 증가함을 확인하였으며, 이는 수계의 오염부하를 가중시킬 것으로 판단되다. 이에 따라 하수관망 내 유출은 강우로 생긴 유량 및 유입오염물질의 농도 증가로 인한 관망이상으로 사료된다. 제안된 하수관망 모니터링 및 이상진단 기법은 도시 유역에서의 비점오염원 관리와 지속적인 모니터링에 있어 효과적으로 사용될 수 있을 것으로 예상된다. Nonpoint source pollution causes leaks and overtopping, depending on the state of the sewer network as well as aggravates the pollution load of the aqueous water system as it is introduced into the sewer by wash-off. According, the need for efficient sewer monitoring system which can manage the sewage flowrate, water quality, inflow/infiltration and overflow has increased for sewer maintenance and the prevention of environmental pollution. However, the sewer monitoring is not easy since the sewer network is built in underground with the complex nature of its structure and connections. Sewer decontamination mechanism as well as pipe network monitoring and fault diagnosis of water network system on system analysis proposed in this study. First, the pollution removal pattern and behavior of contaminants in the sewer pipe network is analyzed by using sewer process simulation program, stormwater & wastewater management model for expert (XP-SWMM). Second, the sewer network fault diagnosis was performed using the multivariate statistical monitoring to monitor water quality in the sewer and detect the sewer leakage and burst. Sewer decontamination mechanism analysis with static and dynamic state system results showed that loads of total nitrogen (TN) and total phosphorous (TP) during rainfall are greatly increased than non-rainfall , which will aggravate the pollution load of the water system. Accordingly, the sewer outflow in pipe network is analyzed due to the increased flow and inflow of pollutant concentration caused by rainfall. The proposed sewer network monitoring and fault diagnosis technique can be used effectively for the nonpoint source pollution management of the urban watershed as well as continuous monitoring system.

Active site phosphoryl groups in the biphosphorylated phosphotransferase complex reveal dynamics in a millisecond time scale

Yu, Tae-Kyung,Yun, Young-Joo,Lee, Ko On,Ahn, Kyung Jun,Suh, Jeong-Yong Elsevier 2012 FEBS letters Vol.586 No.10

<P><B>Highlights</B></P><P>► Phosphoryl groups in the biphosphorylated complex exhibit characteristic dynamics. ► The dynamics occurs in a millisecond time scale at the active site of HPr. ► The time scale is comparable to the phosphoryl transfer rate between EIN and HPr. ► The dynamics of HPr may be important to interact with multiple partner proteins.</P> <P><B>Abstract</B></P><P>The N-terminal domain of Enzyme I (EIN) and phosphocarrier HPr can form a biphosphorylated complex when they are both phosphorylated by excess cellular phosphoenolpyruvate. Here we show that the electrostatic repulsion between the phosphoryl groups in the biphosphorylated complex results in characteristic dynamics at the active site in a millisecond time scale. The dynamics is localized to phospho-His15 and the stabilizing backbone amide groups of HPr, and does not impact on the phospho-His189 of EIN. The dynamics occurs with the <I>k</I><SUB>ex</SUB> of ∼500s<SUP>−1</SUP> which compares to the phosphoryl transfer rate of ∼850s<SUP>−1</SUP> between EIN and HPr. The conformational dynamics in HPr may be important for its phosphotransfer reactions with multiple partner proteins.</P><P><B>Structured summary of protein interactions</B></P><P><B>EIN</B> and <B>HPr</B> bind by nuclear magnetic resonance (View Interaction).</P>

[PE-0057] Genetic Diversity and Genome-wide Association Study of Korean Soybean Landraces(Glycine max(L.) Merr.)

Yu-Mi Choi(Yu-Mi Choi),XiaoHan Wang(XiaoHan Wang),Hye-Myeong Yoon(Hye-Myeong Yoon),On-Sook Hur(On-Sook Hur),Na-Young Ro(Na-Young Ro),Ho-Cheol Ko(Ho-Cheol Ko),Jung-Yoon Yi(Jung-Yoon Yi) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

2009∼2018년 혈액배양으로부터 분리된 Candida 균종의 항진균제 감수성의 경향

Yu-Yean Hwang,On-Kyun Kang,Chang-Eun Park,Moo-Sik Lee,Young-Kwon Kim,Hee Jae Huh,Nam Yong Lee 대한임상검사과학회 2022 대한임상검사과학회지(KJCLS) Vol.54 No.2

Candida is one of the most common causes of bloodstream infections and a leading cause of morbidity and mortality among hospitalized patients. The purpose of this study was to provide important information for formulating empirical treatment plans for candidemia by investigating the antifungal resistance rate of Candida. Among the Candida strains (973 cases) isolated from blood culture tests at the S hospital in 2009∼2018, 4.7% (N=44) comprising the Candida spp. (932 strains) showed resistance to fluconazole. The resistant strains included C. albicans, C. parapsilosis, C. tropicalis, and C. glabrata. In addition Candida spp. (947 strains) showed resistance to amphotericin B (N=6, 0.6%), flucytosine (N=23, 2.4%) and voriconazole (N=24, 3.1%). C. albicans was resistant to fluconazole (N=23, 6.9%) and voriconazole (N=21, 6.0%), The statistical analysis showed that C. albicans and non-albicans Candida species were resistant to fluconazole (P=0.039) and voriconazole (P<0.001). A monitoring system to understand the rate of candidiasis infections in a hospital setting is required. It is also important to make the right choice of the antifungal agent based on drug susceptibility patterns. Therefore, an infection surveillance policy that tracks Candida resistance through regular antifungal susceptibility tests is necessary. 혈류 감염(BSI)의 주요 원인균의 하나로 입원환자에서 이환율과 사망률을 높이는 칸디다의 항진균제 내성률을 조사하여 칸디다 혈증(candidemia)의 경험적 치료 방침에 중요한 정보를 제공하고자 한다. 2009∼2018년 S병원의 혈액배양 검사에서 분리된 Candida 균주(973건) 중 Candida spp. (932 균주)에 대한 fluconazole 감수성 시험결과에서 4.7% (N=44)가 내성(resistant, R)을 보였고 C. albicans, C. parapsilosis, C. tropicalis, C. glabrata에서 내성 균주를 확인하였다. 또한, Candida spp. (947 균주)의 amphotericin B에 대한 감수성 결과에서는 내성(N=6, 0.6%)이 나타났고, 전체 Candida spp. (973 균주)에 대한 flucytosine 감수성 시험에서는 내성(N=23, 2.4%)을 보였다. Candida spp. (768 균주)의 voriconazole에 대한 감수성 시험에서는 내성(N=24, 3.1%)을 보였다. C. albicans는 fluconazole (N=23, 6.9%), voriconazole (N=21, 6.0%)이 내성이고 통계학적으로 C. albicans과 non-albicans Candida species은 fluconazole (P=0.039), voriconazole (P<0.001)로 나타났다. 칸디다 혈증의 감염률을 이해하고 예방하기 위한 감시 시스템이 요구되고 항진균제의 적절한 투여와 치료가 요구된다. 따라서 항진균제 감수성 결과의 모니터링을 통한 칸디다의 내성을 추적하는 감염감시활동 정책이 필요할 것으로 사료된다.

Aurora kinase A induces migration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells

On-Yu Hong,Sang Yull Kang,Eun-Mi Noh,Hong-Nu Yu,Hye-Yeon Jang,Seong-Hun Kim,Jingyu Hong,Eun Yong Chung,Jong-Suk Kim 생화학분자생물학회 2022 BMB Reports Vol.55 No.2

Aurora kinase is a family of serine/threonine kinases intimatelyassociated with mitotic progression and the development ofhuman cancers. Studies have shown that aurora kinases areimportant for the protein kinase C (PKC)-induced invasion ofcolon cancer cells. Recent studies have shown that aurora kinaseA promotes distant metastasis by inducing epithelial-to-mesenchymaltransition (EMT) in colon cancer cells. However, the roleof aurora kinase A in colon cancer metastasis remains unclear. In this study, we investigated the effects of aurora kinase A onPKC-induced cell invasion, migration, and EMT in human SW480colon cancer cells. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) changed the expression levels of EMT markers,increasing α-SMA, vimentin, and MMP-9 expression and decreasingE-cadherin expression, with changes in cell morphology. TPA treatment induced EMT in a PKC-dependent manner. Moreover,the inhibition of aurora kinase A by siRNAs and inhibitors(reversine and VX-680) suppressed TPA-induced cell invasion,migration, and EMT in SW480 human colon cells. Inhibition ofaurora kinase A blocked TPA-induced vimentin and MMP-9 expression,and decreased E-cadherin expression. Furthermore, theknockdown of aurora kinase A decreased the transcriptionalactivity of NF-κB and AP-1 in PKC-stimulated SW480 cells. Thesefindings indicate that aurora kinase A induces migration andinvasion by inducing EMT in SW480 colon cancer cells. To thebest of our knowledge, this is the first study that showed aurorakinase A is a key molecule in PKC-induced metastasis in coloncancer cells.

Probing Target Search Pathways during Protein–Protein Association by Rational Mutations Based on Paramagnetic Relaxation Enhancement

Yu, Tae‐,Kyung,Yun, Young‐,Joo,Lee, Ko On,Suh, Jeong‐,Yong WILEY‐VCH Verlag 2013 Angewandte Chemie Vol.125 No.12

<P><B>Keine Zufallsbekanntschaften</B>: Die Assoziation von Proteinen verläuft über kurzlebige Stoßkomplexe, die je nach ihrer Rolle bei der Bildung des spezifischen Komplexes produktiv oder unproduktiv sein können (siehe Schema). Eine Untersuchung der NMR‐spektroskopischen paramagnetischen Relaxationsverstärkung von komplexierenden Proteinmutanten zeigt, dass produktive Stoßkomplexe direkt beobachtbar und quantitativ lokalisierbar sind.</P>

MK-0677, a Ghrelin Agonist, Alleviates Amyloid Beta-Related Pathology in 5XFAD Mice, an Animal Model of Alzheimer’s Disease

Jeong, Yu-on,Shin, Soo Jung,Park, Jun Yong,Ku, Bo Kyeong,Song, Ji Soo,Kim, Jwa-Jin,Jeon, Seong Gak,Lee, Sang Min,Moon, Minho MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.6

<P>Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive deficits, neuroinflammation, and neuronal death. The primary pathogenic cause is believed to be the accumulation of pathogenic amyloid beta (Aβ) assemblies in the brain. Ghrelin, which is a peptide hormone predominantly secreted from the stomach, is an endogenous ligand for the growth hormone secretagogue-receptor type 1a (GHS-R1a). MK-0677 is a ghrelin agonist that potently stimulates the GHS-R1a ghrelin receptor. Interestingly, previous studies have shown that ghrelin improves cognitive impairments and attenuates neuronal death and neuroinflammation in several neurological disorders. However, it is unknown whether MK-0677 can affect Aβ accumulation or Aβ-mediated pathology in the brains of patients with AD. Therefore, we examined the effects of MK-0677 administration on AD-related pathology in 5XFAD mice, an Aβ-overexpressing transgenic mouse model of AD. MK-0677 was intraperitoneally administered to three-month-old 5XFAD mice. To visualize Aβ accumulation, neuroinflammation, and neurodegeneration, thioflavin-S staining and immunostaining with antibodies against Aβ (4G8), ionized calcium-binding adaptor molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), neuronal nuclear antigen (NeuN), and synaptophysin were conducted in the neocortex of 5XFAD and wild-type mice, and to evaluate changes of phosphorylated cyclic adenosine monophosphate (cAMP) response element binding protein (pCREB) levels, immunostaining with antibody against pCREB was performed in dentate gyrus of the hippocampus of 5XFAD and wild-type mice. The histological analyses indicated that MK-0677-treated 5XFAD mice showed reduced Aβ deposition, gliosis, and neuronal and synaptic loss in the deep cortical layers, and inhibited the decrement of pCREB levels in dentate gyrus of the hippocampus compared to vehicle-treated 5XFAD mice. Our results showed that activation of the ghrelin receptor with MK-0677 inhibited the Aβ burden, neuroinflammation, and neurodegeneration, which suggested that MK-0677 might have potential as a treatment of the early phase of AD.</P>

Epigallocatechin gallate inhibits the growth of MDA-MB-231 breast cancer cells via inactivation of the β-catenin signaling pathway

Hong, On-Yu,Noh, Eun-Mi,Jang, Hye-Yeon,Lee, Young-Rae,Lee, Byoung Kil,Jung, Sung Hoo,Kim, Jong-Suk,Youn, Hyun Jo Spandidos Publications 2017 Oncology letters Vol.14 No.1