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Phenotypic Stability for Selected Traits of Some Cowpea Lines in Nigerian Agro-ecologies
( Mohammed F. Ishiyaku ),( Victoria M. Yilwa ),( Bir B. Singh ),( Olusoji O. Olufajo ),( Aminu A. Zaria ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.2
Eight advanced breeding lines of cowpea [Vigna unguiculata (L.) Walp.] were evaluated in a multi-environment trial for phenotypic stability of grain yield, maturity and grain size. There was highly significant genotype x environment interaction for all traits. Simple correlation coefficient was computed to ascertain the level of relationship between stability parameters and agronomic traits. Line IT93K-452-1 was identified as most stable genotype and can be grown all over the Nigerian cowpea belt. The line IT90K-503-1 was the most unstable genotype in terms of grain yield and was also nonresponsive to the environment. However, it is environment specific. IT93K-452-1 has relatively stable maturity across environments. The result suggests that IT93K-452-1 will mature around the average of 65 days irrespective of the location in Nigeria. Lines IT95K-1455 and IT90K-503-1, whose stability parameter values were high, means that in respect of maturity, they are photo-thermo sensitive. They can mature early or delayed depending on the photo-thermal environment. However, lines such as IT93K-273-2-1, IT93K-129-4, IT93K-452-1 and IT86D-719 are photo-thermo insensitive. Line IT93K-1543 was identified as the most desirable genotype in terms of grain size. This is followed by IT93K-452-1. The line IT90K-503-1 had small but unstable seed size. This work identified IT93K-452-1 and IT93K-273-2-1 as the most stable genotypes for yield and maturity. Grain yield was highly positively correlated with bi and R<sup>2</sup> but was negatively correlated with Sd<sup>2</sup><sub>i</sub>. Maturity was negatively highly correlated with b<sub>i</sub>, W<sub>i</sub>, and CV<sub>i</sub>. Grain size on the other hand is not correlated with any of the parameters.
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Hemoglobin F level in different hemoglobin variants
Akanni E. Olufemi,Oseni B. Sola,Bamisaye E. Oluwaseyi,Raji A. Ajani,Mewoyeka O. Olusoji,Hassan R. Olubunmi 대한혈액학회 2011 Blood Research Vol.46 No.2
Background :Fetal hemoglobin (HbF) levels in different hemoglobin variants in Osogbo, Nigeria, were estimated using two principal methods of estimation using existing information for HbF concentration and distribution of various hemoglobin variants in the area, as well as diagnosed cases of thalassemia. Two hundred and sixty samples collected from HbSS, HbSC, HbAA, HbAS, and HbAC subjects were analyzed. HbF level and hemoglobin type were determined in this study. Methods :The hemoglobin type was determined using cellulose acetate electrophoresis at an alkaline pH, and HbF was determined by the acid elution and alkaline denaturation methods. Results :The mean±SD of HbF in the respective hemoglobin variants was as follows: HbSS, 2.09±1.94%; HbSC, 0.85±0.54%; HbAA, 0.69±0.46%; HbAS, 0.52±0.31%; and HbAC, 0.57±0.26%. The mean HbF level across the hemoglobin variants was statistically significant (P<0.05). Investigating the sex distribution of the HbF level in the studied population revealed a slightly higher mean HbF level in females than in their male counterparts. Conclusion :Within the study population, the HbF level was found to be highest in HbSS and lowest in HbAS. The two methods of estimating HbF are equally reliable, since there was no significant difference between the results obtained from the two methods.
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