고장성 식염수를 이용한 실험적 근육통에서의 통증 양상

손민균,김태민,서정수,김봉옥,윤승호 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.3

수두백신의 역가시험법 표준화를 위한 연구

민경일,백선영,신진호,김재옥,류승렬,민복순,김병국,김도근,김훈,이석호,박순희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

수두(chicltenpo즈) 및 대상포진(shingles)의 예방을 위해 사용되는 수두 생 바이러스 백근은 현재 국내 -외 제조사들이 정상사람 2배체 폐 태아 세포 주들을 이용하여 바이러스를 배양 · 증식시켜 생산하고 있으나, 품질관리를 쒸한 역가 시험에는 t·fRC튼 띤 LB딘EL 세포 주외에 제조사가 설정한 세포 주를 이용하고 있으며, 역파신험 방법인 fH Ui'rFO Plaque 3SSaIT에도 조사별로 다소 차이가 있는 시헌법을 사용하고 있다. 그러므로 일반적으로 세포 주간우 감수성 차이, 계대력(passa딩e level)간의 차이,varicelta-zoster virus(4'2V) diluent medium 등에 의한 차이에 의해 역가시힘 결과에 많은 차이를 나타낼 수 있다. 이에 본 연구에서는 역가 시험 결과꼭 편차를 촉소화 할 수 있는 시헌방법을 극힙하고자 Ok퍼 백신 주와 세로 주간 및 기타 요인에 따른 차이를 비교 분석하고 이를 표준확하여 일관겅있는 국가 및 제조사의 품질관리예 활용하고 나아가 수두 생 바이러스 백신 역가시헌 국가 표준품 거달에 활용하고자 하는데 목적이 운.다. 수두 생 바이러스 빅신의 역가 시험에 영향을 띠칠 수 꿀.는 모민으로 우선 바이러스 흡착에 사용되는 1#Z)·'diluent medium이 가장 큰 오인으로 확인되.고. 현개까기 최종적으로 if4 sucrose, 10% FBS, 0.1% sodiun) rrlonog)utamate in PBS(-), fIH 7.2가 가당 두수글 L'z).「 린luent medium 조성으로 확인되었단. 또한 '·:~l포 주별로 다소의 차이는 잇.으나 최대 바이러스 흡착시간은 90분으론:÷잰착인 되었다. 다른 negati)Fe effect를 보일 수 있는 요인으로 항생물질 및 항츤 제인 fuugiBone이 곡이러스의 역가에 영향을 미치는 것으로 확인되었다 따라서 이러한 항생물 띤 항균제를 standard solid agarose overla?·(5.tO) 시헌방법에는 사용하지 않는 것이 역가 시험의 편차를 줄일 수 있는 요인이라 판단된다. 최적의 조건하데서 Oka 수두 백신 주의 j 종의 세포 쭈에 대한 역가시험 수행 결과 각 세포 주의 감수성의 차이는 크지 않았으나, 반복시헌에 대한 표준편차는 다소 차이를 나타났다. Until now in Korea, the international and domestic vaccine maufacturers produce and sale live attenuated daricella vaccines, used to prevent chickenpox and shinglees, which are propagated in and done the potency test with various originated human diploid embryonic lung cells. Also manufacturers perform in vitro plaque assay with a little different methods to their quality control tests. Therefore, in general, there is slight or big difference among in vitro plaque assay results because of the difference in susceptibility of various originated human diploid embryonic lung cells and in assay methods. The purpose of this study is to confirm what factors occurring deviation to establish the standardized method which minimize deviation intracellularly and intercellularly. Eventually the standadized method will be used in consistent national and manufacturer's quality control and moreover intra and inter laboratory's validation assay of national standard for live varicella vaccine candidate. We confirmed VZV diluent medium was the biggest factor influencing in vitor plaque assay and the excellent composition of VZV diluent medium was final 5% sucrose, 10% FBS, 0.1% sodium monoglutamate in PBS(-), pH 7.2. We confirmed 90 minutes was maximum and optimum VZV adsorption time, but a little different among cells Also we recommended not use antibiotics and antimycotic, fungizone in standard solid agarose overlay assay in varicella potency test. Eventually 5 different originated human diploid embryonic lung cells showed little difference in susceptibility to Oka vaccine strain, but showed a lot of difference in reproducibility.

Antioxidant Activity and Whitening Effect of Lindera erythrocarpa Extract

Min ?Chul Kang,Ryeo Kyeoung Ko,Ju-Yeop Lee,Haeng-Bum Kim,Bong-Seok Kim,Jong-Heon Han,Seong-Hyun An,Gi-Ok Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

농촌병원과 도시 종합병원 외래환자의 수진행동에 관한 비교연구

민현옥,이종숙 中央醫學社 1993 中央醫學 Vol.58 No.11

In order to know the sick role behavior of outpatients who visiting the Hospitals in City and Rural, this questionnaire survey was conducted from Dec, 1990 to Feb, 1991 in the five selected hospitals; 3 general hospitals in Taejeon City al d 2 general hospitals in Chungnam provincial for 876 outpatients. The major findings of this survey were as follows: 1. A total of 49.3 % of the study population were male and female were 50.7 % and average age were 40.14 years old. 2. The average time spent on diagnosis and treatment in hospitals was 138.6 minutes, the spent times were longer in hospitals in city than one in Rural area. 3. The average time spent on diagnosis and treatments by departments was 168.7 min. in internal medicine, 136.1 min. in orthopedic surgery, 104.4 min. in obstetrics and gynecology, 86.8 min. in general surgery, 81.4 min. in pediatrics. 4. The previous medical facility from which patients were transport were 37.2 % in clinic, 16.4 % in hospital, 4.2 % in health center and subcenter and 38.8 % in no transfarted medical facility. 5. The Routes of hospital choice were 30.9 % in patients' own first choice, 35.8 in transferred from other facility on patients' own decision, 26.5 % in recommended by other medical facility. 6. The reasons of hospital choice were 32.1 % in previous experience and familiarity, 26.6 % in bad treatments in previous hospitals, 28.0 % in good facility and equipment. 7. The dissatisfaction against currently treating hospital were 58.9 % in long waiting time, 16.4 % in insufficient explanation of disease, 16.5 % in unkindness, 13.4 % in diagnosis time is too short, 26.6 % in no dissatisfaction. 8. The attitude on medical care delivery system were 34.8 % in acceptable, 23.4 % in not acceptable, 36.5 % in don't know.

상징적 공간 탐사 기법을 이용한 상향적 및 하향적 주의 효과 측정

옥애니,김민식 한국뇌학회 2001 한국뇌학회지 Vol.1 No.1

상징적 공간 탐사과제를 이용하여 시각적 주의의 상향적 및 하향적 요소를 3개의 실험을 통하여 측정하였다. 실험 1에서는 탐사자극 이전에 돌발자극을 제시하여 주의가 상향적으로 유도되도록 조작하였으며, 실험 2에서는 1차 탐색과제로 단순 색으로 정의된 표적자극과 방해자극을 제시하여 주의의 상향적 효과와 하향적 효과 모두를 측정하였으며, 실험 3에서는 탐색과제에서 탐색자극의 상향적 정보 차이를 최소화한 채 하향적 효과를 주로 측정하였다. 실험결과, 상향적 및 하향적 주의효과가 상징적 공간 탐사 기법을 통하여 측정 가능함이 나타났으며, 기존의 연구 결과와 유사한 방식으로 이 두 기제의 시간적 및 공간적 작용 방식에 각기 차이가 있음을 보였다. 이러한 결과는 공간적 주의가 아날로그적 공간 표상 뿐 아니라 상징적인 공간 표상과 같은 다양한 공간 표상 수준에서 작동할 가능성을 시사해 준다. Three experiments were performed to examine bottom-up and top-down attentional effects, using a symbolic spatial probe task. Experiment 1 measured bottom-up factors. Probe responses were faster when the arrow pointed to an abrupt onset location than to blank locations, indicating that symbolic spatial representation is affected by the bottom-up activation of an abrupt onset stimulus. In experiment 2, both top-down and bottom-up attentional effects were measured. Probe results showed that the target location received more attention with both SOA conditions (133msec and 317msec). In experiment 3, we measured top-down control of attention, and found again the benefit of the target location, but only with the long-SOA condition (317msec). The symbolic spatial task in the current study could measure the bottom-up and top-down mechanisms of visual attention that develop differently over time and space. Moreover, these results suggest that spatial attention operates on many different spatial representations such as a symbolic spatial representation as well as an analogical spatial representation.

급성 충수염으로 진단된 예측 불가 염증성 맹장 종물에 대한 외과적 고찰

민경진,백무준,채만규,김성용,이문수,김창호,김재준,송옥평 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: An unexpected inflammatory cecal mass of uncertain etiology was encountered during surgery for presumed appendicitis. In this case, the surgeon couldn't exclude differential diagnosis which were severe appendicitis involving the cecal region, diverticular disease, other inflammatory bowel diseases, and neoplasm. Therefore, this finding leads to a therapeutic dilemma for the surgeon, when making surgical decisions during the operation. The aim of this study was to resolve this dilemma, a retrospective review was undertaken in this study. Methods: A retrospective study was performed to review the management of this problem at Soonchunhyang University Chunan Hospital for eight and half years from July 1991 to December 1999. A review of the emergency operation records identified 50 patients who had undergone ileocecal resection or right hemicolectomy for an inflammatory ileocecal mass of uncertain etiology when operated on for probable appendicitis. Results: Altogether, 39 patients underwent ileocecal resection, and 11 patients underwent right hemicolectomy. The final pathologic diagnosis was appendiceal abscess in 32 patients (63%), cecal diverticulitis in 7 patients (14%), neoplasm in 6 patients (12%), appendiceal phlegmon in 3 patients (6%), ileal tuberculosis in 1 patient (2%) and Anisakiasis in another patient (2%). The neoplasm of the 6 neoplasm patients was ascending colon cancer for 4 patients and cecal cancer for 2 patients. There was no mortality in this group. Conclusion: Although most inflammatory cecal masses are caused by benign disease, all cases in which the intraoperative diagnosis is unclear, any pathologic diagnosis including neoplasm cannot be ruled out. So this suggest ileocecal resection or right hemicolectomy to remove a possible underlying malignancy, and to decrease morbidity and mortality.

부착규조류를 이용한 남한강 하류수계의 생물학적 수질평가

이옥민 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-

The study was the research about the epilithic diatoms and physco-chemical factors at 5 sites of South Han river. The density of Chlorophyll-a showed from site 2 showing the highest value and the rest sites showed 12.9-10.9ug·cm^(-2), its values are a relatively low value from site 2. The values of T-N was 2.494-4.037mg·ℓ^(-1), showing from site 4 the highest value. T-P was 0.017-0.041mg·ℓ^(-1) showed the highest value from site 4. Site 5 from the DAIpo was 81.2, showing the highest value and rest of sites was from 41.6-57.0. When DAIpo was divided into grades from A to D, site 5 was B, the rest sites were C. In the grades of TDI, site 1 and 2 were grade C, site 3 and 4 showed grade D, and site 5 showed grade B. As shown above, DAIpo and TDI did not match in site 3 and 4, and the water was relatively polluted compared to la and Ib in BOD. The difference among BOD DAIpo, and TDI signifies that there has been a change in the indicator community, It can be concluded for DAIpo and TDI to be used together to determine the water pollution because DAIpo is instable with the water quality determination of the water mixed area.

Memory 소자 응용을 위한 BST(Ba_xSr_1-_xTiO_3) 강유전체 박막과 전극과의 계면특성

정민재,조정희,홍석민,김호건,김옥경 漢陽大學校 工學技術硏究所 1997 工學技術論文集 Vol.6 No.1

BaxSr1-xTiO3 powders were prepared by changing stoichiometry of Ba and Sr. After they were calcined and sintered at 1,200℃ and 1,300℃ respectively, BST sputtering targets were made in 3inch mould. For MIM structure capacitors. Pt-based structures such as Pt/TiN/SiO2/Si, Pt/Ti/Si3N4/Si, Pt/SiO2/Si, Ru/SiO2/Si and Ru/Si3N4/Si were used for bottom electrodes, and subsequently, the BaxSrl-xTiO3 thin films were sputtered on them. After the analysis of their structures by XRD, Al was evaporated on the BST films for MIM capacitor. We found that the structures of the films showed good crystallinity above 550℃ from the results of XRD measurements. The largest dielectric constant of films was obtained with 0.7mol% of Ba. The electric properties of the capacitors were very good with Pt/TiN/SiO2/Si electrodes.

Ethylenediaminetetraacetic acid(EDTA)항응고제에 의한 Mean Corpuscular Volume(MCV)변화

홍승민,박영진,최하영,임진,정옥연,이호준,이호영,장숙진,문대수 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.2

It is essential that we put the anticoagulant into the blood specimen of the CBC test. However, the value of some parameters in the CBC test can be changed whenever using some anticoagulants. EDTA anticoagulant is mostly used these days. There are 3 kinds of EDTA, that is K_(2-), K_(3-), and Na_(2-) EDTA. The author studies how many effects each the EDTA gets into the true value of blood specimen for MCV of RBCs. Especially we compared the MCV effect of different concentrations of 3 EDTA anticoagulants on the bias with time and instrument. Some results are gotten. In our results, K_(3-)EDTA anticoagulant shows more satisfactory MCV value than K_(2) or Na_(2-)EDTA. On using concentration of EDTA, 1.5㎎ EDTA/㎖ of blood show more satisfactory MCV value than 7.5㎎ EDTA/㎖ of blood. However, the choice of anticoagulant may be dependant on the different instruments.

오리엔탈리즘을 응용한 유니폼 디자인 연구 : 한국·일본·중국의 전통의상을 중심으로

진경옥,이민정 한국의류산업학회 2003 한국의류산업학회지 Vol.5 No.5

People have been getting interested in the Orientalism and it has come up in various areas due to scientific progress. I designed uniforms, which was simple and western, to show its symbolic meanings in the Orientalism. And I have been trying to generalize uniforms with originality and beauty. I have studied the process of the Orientalism and its changes and transitions, concepts and origins in history. And I applied the styles of Korean, Japanese and Chineses costume on it. I also referred to characters of uniforms, which already had images of Korea, Japan and China. Firstly, Korean styles have a contrast in colors by application of Taegeuk. And they gave lines with dark colors in black one-piece dress. This makes people who are wearing looking slimmer. White ramie top looks fresh and Korean traditional tie called Maedeup is set up for button. Secondly, Japanese styles were mainly Kimono style, one piece. They changed into two pieces designed to be active. So, it has emphasized the image of Kimono with Obi at the waist. Thirdly, Chinese styles basically are Chipao style, which has a Chinese collar and a long skirt, which has opening. But they gradually changed into short skirts and more open in the chest. For one of them, they gave lines in the side of chest, sleeves and skirt to be more natural. The costumes have kept their positions as a part of mass society that grows and changes with speed. And oriental uniforms are not just uniforms any longer only for work. It is quite symbolic like a 'second face' as it rep-resents images for their countries and enterprises.