국내 수집 야생버섯류 추출물의 생리활성 비교

안기홍,한재구,김옥태,조재한,An, Gi-Hong,Han, Jae-Gu,Kim, Ok-Tae,Cho, Jae-Han 한국버섯학회 2021 한국버섯학회지 Vol.19 No.1

국내 자생하는 야생버섯 추출물의 생리활성 성분을 평가하기 위하여 각지에서 수집된 야생버섯 70% 에탄올추출물에 대한 DPPH 라디컬 소거능, 총 폴리페놀 및 총 플라보노이드 함량, 철 환원 항산화능, 환원력의 항산화 활성, 아질산염 소거능 및 야생버섯의 건조시료를 이용하여 베타글루칸 함량을 분석하였다. 본 연구에서 수집된 10종의 야생버섯류 중에서 영지(OK1362) 에탄올추출물의 DPPH 라디컬 소거능(73.2%), 총 폴리페놀 함량(28.9 mg GAE/g) 및 총 플라보노이드 함량(10.0 mg QE/g)철 환원 항산화능(0.134), 환원력(0.155), 아질산염 소거능(56.3%)이 다른 버섯류에 비하여 유의적으로 가장 높았다. 그 이외에도 아까시흰구멍버섯(OK1360), 광대버섯속의 A. lanigera (OK1398), 졸각무당버섯(OK1406)이 높은 항산화능 및 아질산염 소거능을 가지고 있음을 확인하였다. 또한 베타글루칸 함량은 영지(OK1362)가 25.2%를 나타내며 가장 높았으며, 그 외에 구름버섯속의 T. lactinea (OK1457)가 24.5%로 높은 것으로 나타났다. 본 연구의 결과는 국내 자생하는 야생버섯류 중 새로운 천연물 유래 생리활성 물질을 탐색하기 위한 기초자료로서 활용 가능성을 기대한다. The aim of the study was to obtain the extracts of various native wild mushrooms and select the useful resources though biological activity evaluation. The anti-oxidant potential, nitrite scavenging activity, and ��-glucan content of wild mushrooms collected from Eumseong and Bonghwa in Korea were investigated. Based on the results of this study, Ganoderma lingzhi (OK1362) showed the highest DPPH radical scavenging activity (73.2%), ferric reducing anti-oxidant power (0.134), reducing power (0.155), nitrite scavenging activity (53.6%), total polyphenol content (28.9 mg GAE/g), flavonoid content (10.0 mg QE/g), and ��-glucan content (25.2%) when compared to other wild mushrooms sampled in this study. In addition, it was confirmed that Perenniporia fraxinea (OK1360), Amanita sp. (OK1398), and Russula sp. (OK1406) had relatively high anti-oxidant and nitrite scavenging potentials. In conclusion, our results can provide fundamental data for extracting beneficial compounds from wild mushrooms.

지팡이의 높이가 체중 지지분포에 미치는 영향

옥준영,김진우,한우석,한재덕,안덕현 한국전문물리치료학회 2000 한국전문물리치료학회지 Vol.7 No.1

This study investigates the influence of cane length on the weight distribution of the elderly in a standing position. Thirsty participants were evaluated using two different cane lengths based on measurements of distal wrist crease to ground (WC cane), and distance of greater trochanter to ground (GT cane). A limloader was used to determine the weight distribution on the subject. It was found that 6.5% of body weight was supported on a GT cane and 7.7% of body weight supported on a WC cane in a standing position. Results suggests that more weight is distributed on a WC cane than a GT cane in the elderly.

부산광역시 승용자동차 주행중 전신진동 및 소음도 평가

옥치상,모현주,정재열 高神大學校保健科學硏究所 1997 보건과학연구소보 Vol.7 No.-

There are many vibrations and noixes during vehicle use in urban although pavement road. Those are from engin vibarations, tire and wheel related vibrations. And the vibrations of vehicle use cause frequently las variation of road state. This study investivated the whole-body vibration and noise caused as variation of road state and speeds during automobile duiving in Pusan City, Korea Based on the results, exposure to whole body vibration and noise during vehicle use was higher at 70km/h than 50km/h in speed. On the floor of vehicle at speed 50km/h and 70km/h, the the mean values of energy equivalent acceleration and permissible exposure time by vebrational performance were 99.2cm/s²(1512.4 min), 200.3cm/s²(1939.5 min)in x axis ; 132.3cm/s²(1035.9 min),182.3cm/s²(1278.2 min) in y axis;87.1cm/s²(2774.6 min),234.8cm/s²(4196.4 min) in z axis, respectively. And on the seat of vehicle at speed 50km/h and 70km/hr,82.0cm/s²(597.1 min),63.2cm/s²(757.4 min), 157.2cm/s²(1286.8 min) in z axis, respectively. Permissible noise exposure time(PNET) caused during vehicle use were 80hr, 184hr in speed 50km/h and 57hr, 116hr in speed 70kn/h when doors were closed and open,respectively.

봉지종류 및 괘대시기가 ‘원황’ 및 ‘화산’ 배 과실의 수확기 품질에 미치는 영향

정억근,이욱용,안영직,이혁재,황용수,천종필 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

We tested fruits quality and skin coloration of two pear cultivar ‘Wonhwang’ and ‘Whasan’ produced by bagging with different kinds of paper bags in the Cheonan area, Chungnam Province, Korea. The fruits were bagged at 35, 45, 55 or 65 days after full bloom (DAFB) with 3 kinds of double layer paper bag which consisted of grey color, yellow color and newspaper for outer layer. The great extent of light interruption was observed in grey colored outer paperbag among three kinds of paperbag which showed only 0.46% of lowest light transmittance when compared with 43.7% and 40.0% of yellow paper and newspaper, respectively. The development of skin redness (a*) increased with the delay of bagging time in two pear cultivars. But the fruits treated at 65 DAFB showed uneven coloration and excessive development of redness which represented low fruit external appearance value. Uniform flesh firmness was attained at the fruits bagged with grey outer color paperbag regardless of bagging time, although the fruit quality indices including soluble solids and titratable acidity did not change significantly by using different kind of fruit bags and bagging time in two pear cultivar. Based on our results, it was explained that the grey-colored fruit bag had positive effect on the development of skin coloration without any detrimental effect on fruit quality factors in ‘Wonhwang’ and ‘Whasan’ pears.

흰쥐에서 경피적 전기 신경자극이 신경병리성통증에 미치는 영향

유재욱,이은우,김봉옥,이배환,손진훈 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.3

전기자극이 성견 골결손부에 매식된 임플란트 주위조직의 치유에 미치는 영향

심재창,김영준,김옥수,정현주 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.2

Several experimental studies showed that the application of small amounts of electric current to bone stimulated osteogenesis at the site of the cathode and suggested that electrical currents promote osseointegration around dental implants. The purpose of this study was to determine the effect of direct microcurrent to endosseous titanium implants placed in bone defects. The right and left 2nd, 3rd and 4th mandibular premolars in ten mongrel dogs (15kg of weight) were extracted. One month later, Ti-machined screw type implants(3.8 mm diameter×8.5 mm length, AVANA�, Ostem) were placed in surgically created circumferential defect area(width 5mm, depth 4mm). The implants were divided into three groups according to the treatment modalities: Control group- implants without electrical stimulation; Experimental group I- implants with allogenic demineralized freeze dried bone grafting and Experimental group Ⅱ- implants allogenic demineralized freeze dried bone grafting and electric stimulation. The animals were sacrificed in the 4th and 8th week after implant placement and un-decalcified specimens were prepared for histological and histometrical evaluation of bone-implant contact ratio (BIC) and bone formation area ratio (BFA) in defect area. Some specimens at 8 weeks after implantation were used for removal torque testing. Histologically, there were connective tissue infiltration in the coronal part of defect area in control and the experimental group I. whereas direct bone contact was found in the experimental group Ⅱ without connective tissue invasion. Average BIC ratios at 4 weeks of healing were 60.1% in the experimental group Ⅱ, 47.4% in the experimental group I and 42.7% in the control. Average BIC ratios at 8 weeks after implantation were 67.6% in the experimental group Ⅱ, 55.9% in the experimental group I and 54,6% in the control. The average BFA ratio was 84.0% in the experimental group Ⅱ, 71.8% in the experimental group I and 58.8% in the control at 4 weeks, and the BFA ratios were 89.6% in the experimental group Ⅱ, 81.4% in the experimental group I and 70.5% in the control at 8 weeks after implantation. The experimental group Ⅱ showed also significantly greater BIC and BFA ratios compared to the control and the experimental group I (p<0.05). The removal torque values at 8 weeks after implantation were 56 Ncm in the experimental group Ⅱ, 49 Ncm in the experimental group I and 43 Ncm in the control. There was a statistically significant difference among 3 groups(p<0.05) . These results suggest that electrical stimulation improve and accelerate bone healing around endosseous titanium implants in bone defect.

청피에 함유된 복강 파크로파지의 탐식작용 억제 성분

은재순,김대근,소준노,지옥표 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The phagocytic activity of murine peritoneal macrophage was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles. The activity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis, lsosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

중소도시 하수관거의 침입수량 및 수질오염 원단위 산정

김옥형,박승기,정재훈,임경호 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

The volume of wastewater in combined sewers in study areas usually exceeds capacities of the sewer system or the treatment plant during storms. For this reason, combined sewer systems are designed to overflow occasionally and discharge excess waste water. The 6 monitoring sites were selected from two small cities near the Hongsung prefecture during over a rainy season. Monitoring was performed by collecting grab samples and by measuring the rainfall and flow rates during dry and wet seasons. Generally the flow rate of wastewater in combined sewers was rapidly decreased after 23:00 PM and gradually increased from 06:30 AM in all sites during the dry season. Due to monitoring and statistical analysis, the groundwater contributes on sewage volume increase (average 3.4-25.4% more) during experimental periods. Therefore, this research is focus on the investigation of the effects of stormwater and groundwater to combined sewer systems.

효소 처리에 의한 섬유벽 변화의 정량적 연구

김재옥,김철환,박종열,양재경 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.4

지료 조성 공정의 리파이닝 처리 동안에 펄프 섬유에 가해지는 섬유 손상을 방지하기 위하여 효소 리파이닝을 실시하였다. 펄프 섬유에 대한 효소 처리 효과를 정량적으로 측정하고자 효소 처리된 펄프섬유를 공초점레이저현미경(CLSM)을 통하여 물에 팽윤된 섬유의 횡단면 영상을 얻은 후 내부 피브릴화 현상을 화상분석법을 통하여 정량적으로 정하였다. 셀룰라아제 효소가 처리된 섬유는 단섬유 발생, 미세분 형성 등과 같은 기계적 손상이 없이 섬유벽의 팽윤이 세포 내강(lumen)을 향하여 일어나는 것을 확인할 수 있었다. 결론적으로 CLSM과 화상분석을 통하여 효소를 이용한 펄프 섬유의 생물학적 처리는 리파이닝과 같은 기계적 처리를 대체함으로써 제지 공정의 개선과 종이 물성의 향상에 상당히 기여할 수 있음이 입증되었다. This study was carried out for determinating the behavior of enzyme toward internal structure of pulp fibers quantitatively. A commercial enzyme, cellulase, was treated to the radiata pine ECF pulp in New Zealand Differently from the previous studies, the visual observation by Confocal Laser Scanning Microscope(CLSM) and the measurement of cross-sectional dimension of pulp fibers in a wet state by image analysis were made. Through the image analysis, it became evident that fiber wall swelling after cellulase treatment on pulp fibers occurred toward fiber lumen. In addition, it was clearly seen that the increase in physical properties of paper was closely related to internal fibrillation by the cellulase. The pulp fibers treated by the enzymatic treatment could avoid mechanical damages like fiber shortening and fines formation. Thus it was proved that the biological treatment by enzyme on pulp fibers generated internal fibrillation of pulp fibers.

어수리(Heracleum moellendorffii Hance.)의 미숙배로부터 형성된 체세포배의 자엽수에 미치는 Cytokinin의 영향

지선옥,정재동,김창길 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

어수리의 미숙배로부터 체소포배 형성시 자엽의 수적변이에 미치는 cytokinin의 영향을 조사하하였다. 구상형배에 0.2mg/l의 BA, 2ip, kinetin을 각각 처리하여 2주간 배양하였을 때, 다자엽의 형성률은 각각 84.9, 42.6, 32.9%로서 대조구(25.2%)에 비하여 BA>2ip>kinein순으로 높았으며 특히 BA 처리시에 가장 높게 나타났다. 또한 BA를 농도별(0, 0.01, 0.05, 0.1mg/l)로 배발생세포괴에 2주간 처리하였을 때, 다자엽의 형성율은 각각 23.3, 39.7, 69.4, 74.4%로서 농도가 높을수록 증가하는 경향이었다. 0.2mg/l의 BA를 구상형, 심장형, 또는 어뢰형, 자엽시기의 배에 2주간 처리한 경우에 다자엽의 형성률은 각각 92.4, 84.4, 26.3%로서 배발생 초기에 처리될 경우에 더욱 높게 나타났다. In order to investigate the effect of cytokinins on the cotyledonary variability of somatic embryos in Heracleum moellendorffii Hance., somatic embryos were induced from the immature zygotic embryo on MS medium containing 1.0mg/l 2, 4-D, and then transferred to 2,4-D-free and cytokinin-added medium for somatic embryogenesis after 4weeks culture. Polycotyledonary embryos were formed most abundantly(84.9%) on the 0.2mg/l BA medium as compared with the 0.2mg/l 2ip(42.6%) and kinetin(32.9%) media, and it was proportional to BA concentrations(0.01∼0.1mg/l). The rate of polycotyledonary embryo formation increased proportional to the period of BA treatment and also increased more at the globular stage than at the heart, torpedo-shaped and cotyledonary stages.