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Ingrid B¨ohm,Hans H. Schild 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.1
Objective: This study was designed to determine prospectively the expression of the multifunctional CD98 protein in peripheral white blood cells in patients receiving iodinated contrast media (CM) for a computed tomography (CT) examination. Materials and Methods: In 12 adult patients that received non-ionic dimeric CM (iosimenol or iodixanol), the expression of CD98 was analyzed from samples of peripheral white blood cells obtained prior to, one hour, and 24 hours after CM injection by the use of flow cytometry analysis and the use of the direct immunofluorescence technique. Results: Overall, expression of CD98 was significantly downregulated 24 hours after CM injection (51.9%±10.8% vs. 38.8%±16.9%; p < 0.04). Patients that received iosimenol exhibited a more pronounced but not significant decrease of CD98 expression both one hour and 24 hours after CM injection. In an analysis of specific patient responses, CD98 downregulation occurred in eight patients. In two patients, CD98 was upregulated, and in the remaining two patients, expression remained unchanged. No patient acquired an adverse CM reaction. Conclusion: This is the first demonstration that CM may be a regulator of CD98 expression. To determine if upregulation is associated with an increased risk for the acquisition of an adverse CM-induced hypersensitivity reaction and if downregulation is associated without a risk for the acquisition of an adverse CMinduced hypersensitivity reaction, further studies with a larger population of patients are required. Objective: This study was designed to determine prospectively the expression of the multifunctional CD98 protein in peripheral white blood cells in patients receiving iodinated contrast media (CM) for a computed tomography (CT) examination. Materials and Methods: In 12 adult patients that received non-ionic dimeric CM (iosimenol or iodixanol), the expression of CD98 was analyzed from samples of peripheral white blood cells obtained prior to, one hour, and 24 hours after CM injection by the use of flow cytometry analysis and the use of the direct immunofluorescence technique. Results: Overall, expression of CD98 was significantly downregulated 24 hours after CM injection (51.9%±10.8% vs. 38.8%±16.9%; p < 0.04). Patients that received iosimenol exhibited a more pronounced but not significant decrease of CD98 expression both one hour and 24 hours after CM injection. In an analysis of specific patient responses, CD98 downregulation occurred in eight patients. In two patients, CD98 was upregulated, and in the remaining two patients, expression remained unchanged. No patient acquired an adverse CM reaction. Conclusion: This is the first demonstration that CM may be a regulator of CD98 expression. To determine if upregulation is associated with an increased risk for the acquisition of an adverse CM-induced hypersensitivity reaction and if downregulation is associated without a risk for the acquisition of an adverse CMinduced hypersensitivity reaction, further studies with a larger population of patients are required.
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Oh, M.,Elvitigala, D.A.S.,Bathige, S.D.N.K.,Lee, S.,Kim, M.J.,Lee, J. Academic Press 2016 FISH AND SHELLFISH IMMUNOLOGY Vol.58 No.-
Apoptosis is a physiological process that can also participate in host immune defense mechanisms, including tumor growth suppression along with homeostasis and maturation of immune cells. Caspases are known to be involved in cellular apoptotic signaling; among them, caspase-8 plays an important role in the initiation phase of the apoptotic death cascade. In the current study, we molecularly characterized a caspase-8 homolog (designated as HaCasp-8) from Hippocampus abdominalis. The HaCasp-8 gene harbors a 1476 bp open reading frame (ORF) that codes for a protein of 492 amino acids (aa) with a predicted molecular mass of 55 kDa. HaCasp-8 houses the typical domain architecture of known initiator caspases, including the death effector domain and the carboxyl-terminal catalytic domain. As expected, phylogenetic analysis reflected a closer evolutionary relationship of HaCasp-8 with its teleostean similitudes. The results of our qPCR assays confirmed the ubiquitous expression of HaCasp-8 in physiologically important tissues examined, with pronounced expression levels in ovary tissues, followed by blood cells. HaCasp-8 expression at the mRNA level was found to be significantly modulated by lipopolysaccharide, polyinosinic:polycytidylic acid, Streptococcus iniae, and Edwardsiella tarda injection. Overexpression of HaCasp-8 could trigger a significant level of cell death in HEK293T cells, suggesting its putative role in cell death. Taken together, our findings suggest that HaCasp-8 is an important component in the caspase cascade, and its expression can be significantly modulated under pathogen stress conditions in the big-belly seahorse.
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Oh, M.,Bathige, S.D.N.K.,Kim, Y.,Lee, S.,Yang, H.,Kim, M.J.,Lee, J. Academic Press 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.67 No.-
Chemokines are a family of chemotactic cytokines that regulate leukocyte migration. They are classified into four groups namely, CXC, CC, C and CX<SUB>3</SUB>C, based on the formation of a disulfide bridge. Among these, CXC chemokines have been identified as the largest group of chemokines in humans. In this study, we identified and functionally characterized a homolog of CXC chemokine from the big-belly seahorse, Hippocampus abdominalis, and designated it as ShCXCL. The cDNA of ShCXCL composed of a 342-bp open reading frame encoding 113 amino acids (aa). The CXC family-specific small cytokine domain (SCY) was identified from the mature peptide region, which comprised of a conserved CXC motif. As ShCXCL lacks an ELR (Glutamic acid-Leucine-Arginine) motif, it belongs to ELR<SUP>-</SUP> subfamily. The recombinant ShCXCL protein strongly induced the nitric oxide (NO) production in macrophage cells (RAW 264.7 cell line) and showed the chemotactic effect on flounder peripheral blood leukocytes. Tissue profiling showed a ubiquitous expression pattern in all examined tissues, with a high abundance in spleen. The up-regulated mRNA expression pattern of ShCXCL was observed in blood and kidney tissues after immune stimulation by live bacteria, such as Streptococcus iniae and Edwardsiella tarda, and mitogens, such as lipopolysaccharides (LPS) and polyinosinic:polycytidylic acid (poly I:C), suggesting its important role in host immune defense against microbial infection.
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Oh, M.,Lee, J.J.,Lee, J.K.,Kim, H. Elsevier Sequoia 2014 JOURNAL OF ALLOYS AND COMPOUNDS Vol.585 No.-
The electrical characteristics and carrier transport mechanism of Mg-doped p-GaN treated with the electrochemical potentiostatic activation (EPA) method was investigated. The EPA method resulted in lower H concentration up to 56% with a subsequent increase in hole concentration and improved ohmic contacts. The temperature-dependent electrical resistivity of the reference p-GaN followed a ~T<SUP>-¼</SUP> dependence, i.e., hopping conduction, while that of the EPA-treated sample began to follow a ~T<SUP>-1</SUP> behavior involving band conduction. This was due to the reduced density of deep-level states associated with the Mg-H complex, e.g., from 1.28x10<SUP>19</SUP> to ~7.50x10<SUP>18</SUP>cm<SUP>-3</SUP> after EPA treatment. The increase in the hole concentration was responsible for the enhanced ohmic contact via tunneling.
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Oh, M.,Umasuthan, N.,Elvitigala, D.A.S.,Wan, Q.,Jo, E.,Ko, J.,Noh, G.E.,Shin, S.,Rho, S.,Lee, J. Academic Press 2016 Fish & Shellfish Immunology Vol.49 No.-
<P>Ferritins play an indispensable role in iron homeostasis through their iron-withholding function in living beings. In the current study, cDNA sequences of three distinct ferritin subunits, including a ferritin H, a ferritin M, and a ferritin L, were identified from big belly seahorse, Hippocampus abdominalis, and molecularly characterized. Complete coding sequences (CDS) of seahorse ferritin H (HaFerH), ferritin M (HaFerM), and ferritin L (HaFerL) subunits were comprised of 531, 528, and 522 base pairs (bp), respectively, which encode polypeptides of 177, 176, and 174 amino acids, respectively, with molecular masses of similar to 20-21 kDa. Our in silico analyses demonstrate that these three ferritin subunits exhibit the typical characteristics of ferritin superfamily members including iron regulatory elements, domain signatures, and reactive centers. The coding sequences of HaFerH, M, and L were cloned and the corresponding proteins were overexpressed in a bacterial system. Recombinantly expressed HaFer proteins demonstrated detectable in vivo iron sequestrating (ferroxidase) activity, consistent with their putative iron binding capability. Quantification of the basal expression of these three HaFer sequences in selected tissues demonstrated a gene-specific ubiquitous spatial distribution pattern, with abundance of mRNA in HaFerM in the liver and predominant expression of HaFerH and HaFerL in blood. Interestingly, the basal expression of all three ferritin genes was found to be significantly modulated against pathogenic stress mounted by lipopolysaccharides (LPS), poly I:C, Streptococcus iniae, and Edwardsiella tarda. Collectively, our findings suggest that the three HaFer subunits may be involved in iron (II) homeostasis in big belly seahorse and that they are important in its host defense mechanisms. (C) 2016 Elsevier Ltd. All rights reserved.</P>
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Rafal Ohme,Manabu Mori 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.7
In the first part we aim to present a new tool to better understand implicit consumer associations, perceptions and impact. The second one is to show how this tool uncovers new and often counterintuitive insights regarding emotional percepts of soccer megastars, including Lionel Messi. BIOCODE™ is a reaction time based method, determines the strength of implicit, i.e. instinctive, immediate, automated or emotional conviction people have to things they say, such as perceptions of a brand or a celebrity, reactions to an ad, liking of a product or intent to vote for a political candidate. It captures how consumers are impacted by brands, ads, products, packages and concepts in contrast to what they overtly say in a declarative, more considered, explicit realm. In essence, we ask to answer (on-line or central location) simple questions about a brand, a person or a product. Consumers’ explicit and rational statements are important but assessing those responses in the context of the time their brains need to produce an answer gives a new perspective and competitive edge. The standardized reaction time index reveals consumers’ true and unbiased reactions. Importantly, these implicit emotional reactions tend to predict actual behavior closer than explicit rational declarations. The Implicit Association Test (IAT) - the first method based on assessing reaction times - was developed in 1998 by Anthony Greenwald to finally capture racial prejudice and other sensitive issues. Before IAT traditional paper & pencil questionnaires due to overt or hidden distortions had hard time proving the existence of racism. What is the cognitive mechanism beyond reaction time based methodology? By recording how much time a consumer’s brain needs to produce an indication of an attitude or preference we discover how easily accessible (and thus how instrumental) such emotion is. The quicker the indication is, the more accessible it gets. The foundations of this neuropsychological phenomena were first described by Donald Hebb in his ‘Consolidation of the Memory Trace’ theory (1948) and then refined by Russell Fazio in his ‘Attitude Accessibility’ model (1989), Daniel Schacter’s ‘Implicit Memory’ theory (1992) as well as Mahzarin Banaji & Anthony Greenwald in their concept of ‘Implicit Social Cognition’ (1994). Rafal Ohme and his team began working with the original, academic form of IAT. Their goal was to bridge the use of the tool to market research applications. Now with over 15 years of subsequent R&D in this area, they have created simplified, user friendly research applications that are unequaled in their ability to measure previously unanswerable questions about the degree of emotional valence or “felt intensity” that is associated with what people say. BIOCODE™ is the second generation of latency measures. Comparing with IAT - the first generation of academic reaction time methods it is: easier, simpler, clearer, looks better and there is no need for repetitions which saves a lot of precious on-line time. BIOCODE™ is based upon highly refined technology that calibrate individual response speeds and eliminate biasing variables. The technology includes: noise reduction algorithm; quality control module; context procedures; calibration. It secures the highest validity of measurements. In the test - retest validation conducted on 11 studies held internally and externally in 2009-2013 the correlations obtained ranged from r = 0,840 to r = 0,960 (conducted on various target groups of high incidence that met all the criteria for the test; demographic characteristics were controlled and groups were homogenous. Together with Manabu Mori from Rakuten Research - one of the top on-line research company - Rafal Ohme have designed the first ever cross-cultural reaction time test on soccer celebrities. Nearly 900 on-line respondents from three continents: South America, Europe and Asia were asked to indicate (on a computer screen, using a regular mouse or a key-pad) whether they agree or disagree (5-point Likert’s scale) with the attitudinal statements on specific personality traits of soccer celebrities, eg. hard working, talented, famous, loving their country. This explicit rational response has been accompanied by implicit emotional reaction. The aim of the study was to by-pass the “rationality bias” and reveal true emotional reactions about soccer celebrities including: Leo Messi, Christiano Ronaldo, Wayne Rooney, Neymar jr, Shinji Kagawa. The selected findings will be disclosed during the presentation. Concluding, BIOCODE™ is a sensitive detector of consumer ‘lip service’ that is often driven by benefit of the doubt, political correctness and simple deference to leadership brands. If we want to understand consumers, it serves to know the gap between what people say and how they feel. It is a very promising, fast growing method with established advanced applications for copy testing, tracking, brand strategy, political polling, product, package and concept testing worldwide. Moreover it is effective as a module embedded within more traditional surveys for providing a seamless integrated perspective on both explicit and implicit aspects of consumer behavior to enrich our understanding of what consumers truly feel and what drives their behavior.
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