연령에 따른 흰쥐 망막 속얼기층의 미세구조 변화

오원영,고정식,양남길,안의태,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

이 실험은 연령에 따른 흰쥐 망막 속얼기층의 미세구조적 변화를 관찰하기 위하여 시행하였다. 실험동물로는 생후 3개월(어린 흰쥐), 12개월(젊은 흰쥐) 및 30개월(늙은 흰쥐)된 Fisher계통 344흰쥐를 사용하였다. 각 동물은 4% chloral hydrate로 마취한 후 2.5% glutaradehyde-1.5% paraformaldehyde로 관류 고정한 다음, 안구를 적출하여 톱니둘레를 다라 절단한 후 시신경원반외측의 일정부위에서 망막조직을 떼어내었다. 떼어낸 조직은 2.5% glutaradehyde- 1.5% paraformaldehyde 고정액에 다시 고정한 후, 1% osmium tetroxide액에 또 다시 고정하였다. 고정된 조직은 ethyl alcohol과 acetone으로 탈수한 후 araldite혼합액에 포매하였다. 포매된 조직은 LKB-V ultratome으로 얇은 절편을 작성하여 uranyl acetate와 lead citrate로 염색한 후 JEM 100CX-Ⅱ 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1. 무축삭신경원의 세포질돌기는 세포질의 전자밀도, 연접소포의 종류 및 양에 따라 4종류 구분되었다. 2. 속얼기층에서 관찰되는 신경연접의 형태는 연접리본복합체외에 4종류의 일반신경연접을 관찰할 수 있었으나, 연령증가에 따른 연접형태의 미세구조적 변화는 관찰할 수 없었다. 3. 연령이 증가함에 따라 부챗살아교세포의 상대적 영역이 넓어졌다. 4. 연령이 증가함에 따라 무착삭신경원과 신경절세포의 가지돌기안에 수초구조, 용해소체, 잔여소체가 자주 관찰되었다. 이상의 결과를 종합해 보면 흰쥐의 망막속얼기층은 연령이 증가하면 무축삭신경원과 신경절세포는 기능이 점점 약해지므로 부챗살아교세포의 영역이 상대적으로 더 넓어진다고 생각된다. Morphological differences among the inner plexiform layers at different age groups have been studied in young(three months old), adult(twelve months old) and old(thirty months old) Fisher stain 344 rats. Under anethesia, 2.5% glutaraldehyde- 1.5% paraformaldehyde solution was perfused through the heart. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde- 1.5% paraformaldehyde, and in 1% osmium tetroxide followed by fixation embedded in aralditse mixture. Ultrathin seciton were statined with uranyl acetate and lead citrate solution and were observed with JEM 100CX-II electron microscope. The results were as follows: 1. Four types of amacrine cells were found according to their electron densities, and kind and amount of the synaptic vesicles, were found, i.e., dark amacrine cell with clear vesicles(Al cell), light amacrine cell with clear vesicles(A2 cell), dark amacrine cell with clear and granular vesicles(A3 cell) and light amacrine cell with clear and granular vesicles(A4 cell) 2. In the inner plexiform layer, there appear two types of synapses : 1) synaptic ribbon complex and 2) conventional synapses. Conventional ones exhibited four different types, showing, i.e., prominent presynaptic density(type 1), prominent postsnaptic density(type 2), prominent pre-and postsynaptic densities(type 3), and lesser prominent pre-and postsynaptic densites. 3. Territories of the Muller cells were expanded during aging. 4. Myelin figures, lysosomes, lipofuscin granules and lipid droplets were frequently observed in the amacrine and ganglion cells of the adult and old rats. From the above results, it was suggested that functions of the bipolar, amacrine and ganglion cells were diminished gradually, whereas territories of the Muller cells were expanded during aging.

Zn₁-xFexSe의 고유결함에 관한 연구

박남천,송재홍,채건식,오재근,김영국,손인호 慶南大學校 附設 基礎科學硏究所 1995 硏究論文集 Vol.7 No.-

We studied the native defects of dilute magnetic semiconductors Zn₁-xFexSe epilayer by using SAW device. The energy gap of Zn₁-xFexSe for each epilayer(x=0.0213, 0.0915) was 2.57eV, 2.74eV at room temperature (294K). The trap level for x=0.0213, 0.0915 were calculated by measuring Transverse Acoustoelectric Voltage(TAV). Also we can calculated cross section.

FRF를 이용한 평판 구조물의 구조손상 규명기법

김남인,이우식,권오양 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.2

주파수 응답함수 (FRF)를 이용하여 평판 구조물에 존재하는 손상을 규명하기 위한 새로운 구조손상 규명기법을 소개하였다. 본 연구의 구조손상 규명기법은 손상된 평판의 운동방정식으로부터 유도되었으며 손상 분포함수를 이용하여 손상의 특성을 나타내었다. 본 연구의 구조손상 규명기법의 주요 특징은 다음과 같다. 첫째, 손상된 구조물에 대한 모드 데이터를 요구하지 않으며, 둘째, 가진주파수와 측정점을 적절히 다수 선택함으로써 손상규명에 요구되는 관계식을 확보하는데 보다 유리하다. 몇몇 수치모의실험을 통하여 본 연구에서 제안한 구조손상 규명기법의 적용 가능성을 검증하였다. In this study, an FRF-based structural damage identification method (SDM) is proposed for plate structures. The present SDM is derived from the partial differential equation of motion of the damaged plate, in which damages are characterized by using a damage distribution function. The appealing features of the present SDM include the following. First, the modal data of the damaged structure are not required. Secondly, a sufficient number of information can be generated from the measured FRFs by simply varying excitation frequencies and response measurement points. The feasibility of the present SDIM is verified through some numerically simulated damage identification tests.

태권도 수련 프로그램 적용에 따른 정신력 효과

김응식,윤오남,선석령,구희성 朝鮮大學校 스포츠科學硏究所 2003 스포츠科學硏究 Vol.14 No.-

This study had a purpose to define an effect of mental strength according to the application of Taekwondo Training Programs. In order to achieve this study objective, the Delphi Technique Analysis and the Quantitative Research were carried out. For this purpose, firstly, experts' opinions about the programs which were practiced in the Taekwondo gym was analyzed. Secondly, the background variable and training level, the training programs, and the difference of Taekwondo mental strength were analyzed. And thirdly, the relations between the Taekwondo training level, the training program, and the Taekwondo mental strength, etc. were analyzed. The analysis of Taekwondo training programs by the DeIphi Technique was performed by means of the 3rd analysis with 30 experts. And as for the Quantitative Research, the study subjects were targeted for the total 265 pupils composed of 224 boys and 41 girls who were selected by the Group Random Sampling. This study took the primary school students in 4-6 years as the population who were training Taekwondo in the gym which was located in Gwangju. The measurement instrument for research survey consisted of five (5) question items for background variables, three (3) question items for training level, three (3) question items for training programs, and twenty-five (25) question items for Taekwondo mental strength, that is, which totaled thirty-six (36) question items in all. As for the relationship model settings between the related variables, gender, school year, income level, academic records, training motive variables were established. As for the independent variables, the medium variables for Taekwondo training level were set up by the training programs. And, the Taekwondo mental strength variables were established for the dependent variables. The data which were collected by using questionnaires were dealt with according to the research objectives. As for the statistical techniques for analysing the treated data, skill statistics, unitary variance analysis, multiple regression analysis were employed. As for the relation of mental strength according to the application of Taekwondo training programs based on the method and procedure of this study, the following results were obtained in accordance with the basis of the Delphi Technique Analysis and result of the Quantitative Research. First, in the program analysis through the Delphi Technique, it was investigated that the following factors were achieved. In the body-centered programs, factors such as curiosity, creative power, interest, etc. were achieved. In the mind-centered programs, factors such as social nature, character, etiquette. sense of cooperation, creative power, and interest etc. were achieved. In the play-centered programs, factors such as meditation, personal relations, creative power, self-esteem, and reliance etc. were achieved. Second, in the differences of Taekwondo programs on the basis of the background variables, school year and income level showed the statistically significant differences in the body-centered and the mind-centered programs among the types of Taekwondo training programs. And the statistically significant difference was shown about the income level in the play-centered programs. This result showed the fact that, in training Taekwondo, pupils showed differences for adapting themselves to the contents of body-, mind-, and play-centered programs according to the contents of programs based on school year and income level which were parts of the background variables. Third, in the relations between the Taekwondo training level and the programs, for the great influence which they had on the body-centered programs about the period, frequency, and the intensity. pupils could adapt themselves well to the training as the period became longer in the body-centered programs. They could also adapt themselves well to the high-intensity training when they were not absent and trained more frequently. As for the period, about the thing which it influenced the mind-centered programs, as they were trained longer in the mind-centered programs, the training period became longer. Also, as for the period and frequency. it was considered that what influenced the play-centered programs was the fact that, as pupils were trained longer, the training period became longer in the play-centered programs. And they seemed to feel more pleasure and interest as the training period became longer and when they were not absent, and when they were trained more frequently. Fourth in the relation between the Taekwondo training level and the mental strength, for the period and the frequency which belongs to the training level, the thing which had significant impact on the sub-factors of the mental strength such as patience, courage, and etiquette was the fact that patience, courage and etiquette which were the sub-factors of mental strength were improved as the Taekwondo training period was longer, and they were trained more frequently. Fifth, in the relation between the type of Taekwondo program and the mental strength, patience, courage, and etiquette was highly improved in the body-centered programs. And courage was highly improved in the mind-centered programs. Also, what had significant impact on patience, courage and etiquette was the body of the Taekwondo program type in the play-centered programs. Likewise, for the mind- and play-centered programs, the mental strength such as patience, courage and etiquette was highly improved as they were trained more frequently.

點播粒數에 따른 靑刈大豆의 生育反應 및 飼料成分變化

趙南棋,吳章植,朴良門,宋昌吉 濟州大學校亞熱帶農業硏究所 1997 亞熱帶農業硏究 Vol.14 No.-

本 硏究는 濟州道에 있어서 點播粒數에 따른 濟州在來大豆의 生育反應 및 飼料成分에 미치는 影響을 究明하기 위하여 1996年 5月 10日부터 8月 16日까지 遂行하였던 結果를 要約하면 다음과 같다. 1. 草長은 4粒區에서 115cm로 가장 길었으며, 1粒區에서 110cm로 가장 짧았다(Y=-0.527X^2+3.885X+107.110). 2. 莖直徑, 分枝數, 葉數 等의 形質은 點播粒數가 많아질수록 減少되는 傾向이었다. 3. 生草收量은 4粒區에서 10a當 3633kg으로 가장 많았으며, 3粒과 5粒區는 中間이었고, 1粒區에서는 2349kg으로 가장 적었다.(Y^*=-125.088X^2+995.387X+1510.790) 4. 乾草收量은 4粒區에서 839kg으로 가장 많았으며, 5粒과 6粒區에서는 中間이었고, 1粒區에서는 509kg으로 가장 적은 편이었다.(Y^*=-23.839X^2+221.255X+279.720) 5. 組蛋白賃과 粗脂肪은 點播粒數가 많아질 수록 增加하였으며, 粗纖維와 粗灰分은 減少되었다. 6. 草長은 生草收量과 正의 相關을 나타내었고, 莖直徑·分枝數·葉數·葉重 等의 形質과는 高度로 有意한 正의 相關을 나타냈으며, 粗焉白質과는 負의 相關을 나타냈다. The study has been conducted to clarify the effects of number of seeds per hill in dibbling on the growth and feed composition of Cheju Local Soybean from May 10 to August 16 in Cheju-Do. The results obtained are summarized as follows ; 1. Plant height when four seeds per hill in dibbling(115cm) was the longest, while one seeds per hill in dibbling(110cm)was the shortest(Y=-0.527X^2+3.885X+107.110). 2. The diameter of stems, number of branches and number of leaves per plant decreased with the number of seeds per hill in dibbling increased. 3. Fresh yield per 10a when four seeds per hill in dibbling(3633kg) were the greatest and three seeds per hill in dibbling, when five seeds per hill in dibbling were intermediate but one seeds per hill in dibbling(2349kg) was the lightest. (Y^*=-125.088X^2+995.387X+1510.790) 4. Dry yield per 10a when four seeds per hill in dibbling(839kg) were the heaviest and when five, six seeds per hill in dibbling were intermediate but one seeds per hill in dibbling(509kg) was the lightest(Y^*=-23.839X^2+221.255X+279.720). 5. Crude protein and crude fat increased with the number of seeds per hill in dibbling increased, but crude fiber and crude ash decreased. 6. There was positive correlation between plant height and fresh yield, and further highly positive correlation between plant height and the diameter of stems, number of branches, number of leaves, or the weight of loaves and negative correlation between plant height and crude protein.

지역병원에서 다발성 손상환자 후송시에 문제점

김영식,안무업,임경수,황성오,조남천,강성준 대한응급의학회 1992 대한응급의학회지 Vol.3 No.2

A prospective review of 53 multiple injured patients(ISS≥16) initially treated at local community hospitals and subsequently transported more than 50km to referral emergency center was completed. All injury mechanisms were blunt, and among them traffic accidents occupied the 54.7%. Revised Trauma Score ranged from 0 to 12 (mean, 10.4) and Injury Severity Score ranged from 16 to 50 (mean, 24.3). No prior notification of patient transfer to the referring facility were received in 52(98.1%) cases, and absent or very incomplete record were evident in 44 (83.0%) cases. The quality of care during initial stabilization and transport was evaluated by ATLS guideline for airway management, cervical immobilization, breathing support, treatment of shock, fracture immobiliaztion, neurologic evaluation, secondary assessment, life-threatening deficiencies were identified in 10(18.9%) patients and serious deficiencies in 32(60.4%). Most frequent departures from these standards involved failure to place a nasogastric tube before transport(58.5%), inadequate breathing support(30.2%), inadequate cervical immobilization(24.5%) and inadequate shock treatment(17.0%). This study demonstrates the need for education of emergency physicians about priorities in trauma management and ATLS courses. Furthermore guidelines for transport and interhospital communication must be established in rural area.

OK-432(Picibanilⓡ)와 외과적 절제술을 이용한 선천성 림프관종의 치험례

김일규,이성호,오성섭,최진호,오남식,김왕식,임영일,양동환 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Lymphangiomas are relatively rare benign tumors of the lymphatic system, characterized by congenital lymphatic malformation such as anastomosis or obstuction of the lymphatic channels. There are most frequently diagnosed in children younger than 3years. In contrast to that of the hemangioma, the sex distribution of the lymphangioma is nearly evenly divided. The head and neck lymphangioma represents about 70∼75% of all lymphangiomas, and they are difficult to manage. Spontaneous regression is rare, and rapid intermittent enlargement occurs secondary to infection or trauma. Enlargement may cause serious sequelae such as airway obstruction, feeding difficulties, and cosmetic problems. Treatments previously used for lymphangiomas include surgical excision and intralesional injection of sclerosants. Problems associated with surgical excision include the risk of cosmetically unacceptable scarring and the risk of damage to surrounding vital stricture and the high risk of incomplete excision. The sclerosants previously used have numerous other local and systemic side effects. This report describes a case that was successfully treated using OK-432 as a new sclerosant drug and secondary surgical excision for congential cavenous lymphangioma extensively enlarged to tongue, mouth floor and submandibular region.

악관절 융기 증강술을 통한 습관성 악관절 탈구환자의 치험례

김일규,신주호,오성섭,최진호,김형돈,오남식,김의성 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

Recurrent mandibular dislocation is frequent morbidity of temporomandibular joint relatively. There are many etiologic causes in TMJ disorders but, difficult to find obvious one. Various treatment methods have been utilized for a mandibular dislocation. It is categorized into two groups broadly - nonsurgical or surgical methods. The basic rationale of the surgical method is to allow free movement of the condyle by reducing height of articular eminence or to limit anterior excessive movement of the condyle by increasing height of articular eminence or soft tissue anchoring procedure. In this case, 69 year-old woman was treated by augmentation of the articular eminence with mandibular symphysial bone graft leading to osteosynthesis without difficulty. As a result, favorable postoperative outcome was obtained functionally without any complication or recurrence.

지치 발거 후 안면부에 발생한 방선균증의 치험례

하지영,김일규,오성섭,최진호,오남식,차상권 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.1

Actinomycosis is chronic, granulomatous, suppurative and fibrosing disease caused by Actinomyces. Actinomyces are anaerobic, G(+), non-acid-fast, branched, filamentous bacteria. The most commonly found microorganism is Actinomyces israelii. Common site for isolation of actinomyces are dental plaque, dental caries, calculus, and tonsillar crypt. A breach in the integrity of the mucosa by direct trauma or following a fracture, tooth extraction, root canal therapy or some intraoral surgical procedure is thought to be the most likely portal of entry. This is a case report of 23 years old male with cervicofacial actinomycosis developed after extraction and treated with surgical excision and antibiotics.

구강악안면 영역의 치성 감염 환자에 대한 세균학적 연구

김일규,윤승환,오성섭,최진호,오남식,김의성,이성호,배수환,강문수 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Oral & maxillofacial infections are most commonly odontogenic in origin. Although such infections are usually self-limiting, they may occasionally spread deeply into fascial spaces or planes far from the initial site of involvement. If early diagnosis and appropriate therapy is delayed, complications such as mediastinal extension, retropharyngeal spread and airway obstruction could happen to the patients. For the study of the microbiology, we have retrospectively analysed the oral & maxillofacial infected patients in the Dept. of Oral & Maxillofacial Surgery, In-Ha University Hospital from 1997 September to 2000 April. The results were as follows 1.The male patients were more common than female, with male 61.9% and female 38.1%. 2.Dental originated infections were most common cause with the incidence of 62%. 3.Most common fascial space involved was buccal space 42cases(37.2%) followed by submandibular space 13cases(11.5%), infraorbital space 13cases(11.5%), masseteric space 11cases(9.7%) , periapical abscess 11cases(9.7%) . 4.The causative organisms isolated from the pus culture were Gram Positive Bacterial species, which were 46cases(31.9%) of Streptococcus viridans, 16cases(8.6%) of α and β-hemolytic streptococcus, 4cases(3.1%) of Strep.-group D non enterococci, 7cases(5.1%) of Staphylococcus Coa. Neg., 5cases(3.9%) of Staphylococcus aureus, 3cases(2.3%) of Enterococcus faecalis, 1case(0.8%) of Bacillus species, 1case(0.8%) of Peptostreptococcus, 1case(0.8%) of Clostridium and Gram negative bacterial species, which were 4cases(3.1%) of Acinetobacter baumannii, 2cases(1.6%) of Pseudomonas aeruginosa, 2cases(1.6%) of Burkholderia cepacia, 1case(0.8%) of Neisseria species, 1case(0.8%) of Klebsiella pneumoniae, 1case(0.8%) of Klebsiella oxytoca, 1case(0.8%) of Escherichia coli. 5.In drug sensitivity test, high resistant tendency was found in Penicillin system(Penicillin G 83.3%, Ampicillin 60%) and Aminoglycosides (Gentamycin 50%, Tobramycin 45.5%), but tertiary Cephalosporin system(Cefoperazone 9.1%, Ceftazidime 18.2%), and glycopeptides system (Teicoplanin 0% , Vancomycin 0%) showed lower resistancy.