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Molecular characteristics of canine parainfluenza viruses type 5 (CPIV-5) isolated in Korea.
Oem, Jae-Ku,Kim, Seong-Hee,Kim, Yeon-Hee,Lee, Myoung-Heon,Lee, Kyoung-Ki Canadian Veterinary Medical Association = Associat 2015 Canadian journal of veterinary research Vol.79 No.1
<P>Three canine parainfluenza viruses type 5 (CPIV-5) were isolated from lung tissues of 3 Korean dogs with mild pneumonia between 2008 and 2009. The isolates were fully sequenced and compared with published reference sequences. The size of the genome was 15 246 nucleotides long and no remarkable differences were found when compared with previously published reference sequences. In phylogenetic analysis based on the F and P genes, parainfluenza virus 5 (PIV-5) strains were divided into at least 3 subgroups. Three CPIV-5 strains were clustered with CPIV-5 T1, H22 and 78524 strains. All PIV-5 strains were independent of the host species, geographical distribution, and the isolated period.</P>
OEM, Jae-Ku,LEE, Kwang-Nyeong,ROH, In Soon,LEE, Kyoung-Ki,KIM, Seong-Hee,KIM, Hye-Ryoung,PARK, Choi-Kyu,JOO, Yi-Seok Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.11
<P>Nine isolates of rabbit hemorrhagic disease virus (RHDV) were used for the genetic characterization of RHDV strains collected from rabbits in Korea between 2006 and 2008. A phylogenetic analysis of the complete VP60 region was performed and the sequences were divided mainly into two groups. The one group consisted of original RHDV and the other contained antigenic variant strain known as RHDVa strains. Most of the Korean isolates clustered with Chinese RHDV strains and belonged to the RHDVa subtype. A comparison of the amino acid sequences among RHDVa strains and original RHDV strains revealed significant substitutions of two amino acids in the A region, two in the B region, two in the F region, and nine amino acids in the E region. Taken together, the recent RHDVa strains have gradually replaced the original RHDV and are the predominant strains in Korea.</P>
Molecular characterization of a Korean bovine parainfluenza virus type 3 isolate
Oem, J.K.,Lee, E.Y.,Lee, K.K.,Kim, S.H.,Lee, M.H.,Hyun, B.H. Elsevier Scientific Pub. Co 2013 Veterinary microbiology Vol.162 No.1
Bovine parainfluenza virus type 3 (BPIV-3) was isolated from Korean native cattle that presented clinical signs of mild pneumonia. The complete genome of a representative isolate (12Q061) was sequenced. The newly identified strain, which was found to be distinct from the previously reported genotypes A (BPIV-3a) and B (BPIV-3b) and closely related to the Chinese strain SD0835, was tentatively classified as genotype C (BPIV-3c). Our results suggest a relationship between BPIV-3 genetic variation and the geographic location of its isolation. Identification of these new BPIV-3 genotypes may facilitate the development of improved diagnostic methods and vaccines. This is to our knowledge the first report of the identification and molecular characterization of BPIV-3 in Korea.
Serological and virological investigation of pestiviruses in Korean black goats
Oem, Jae-Ku,Lee, Eun-Yong,Byun, Jae-Won,Kim, Ha-Young,Kwak, Dong-Mi,Song, Hee-Jong,Jung, Byeong-Yeal The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.2
Blood samples were collected from 672 goats in 60 farms from five provinces of Korea between November 2009 and August 2011. The prevalence of antibodies to pestiviruses was investigated. The examination for antibodies was performed using an enzyme-linked immunosorbent assay (ELISA) detecting antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV). All blood samples were screened using reverse transcription-polymerase chain reaction (RT-PCR) with primer pairs specific to common pestivirus genome regions. The observed individual seroprevalence was 1.49% and herd seroprevalence was 11.67%. Also, the specific genomes to pestiviruses were detected in 3 out of the 915 clinical samples (0.45%). Based on the nucleotide sequence data, detected pestiviruses were belonged to two BVDV type-1 and one BVDV type-2. The pestivirus infection has been occurred among Korean black goats. However, our results indicate that the prevalence of pestiviruses in black goats was not significantly higher on farms with cattle.
Bovine papular stomatitis virus (BPSV) infections in Korean native cattle.
Oem, Jae-Ku,Lee, Eun-Yong,Lee, Kyoung-Ki,Kim, Seong-Hee,Lee, Myoung-Heon,Hyun, Bang-Hun The Society ; Maruzen Co. [distributor] 2013 The Journal of veterinary medical science Vol.75 No.5
<P>An outbreak of a disease with parapox-like symptoms was reported in South Korea in April 2012. Three of 45 Korean native cattle, age 20-24 months, were affected. Parapoxviruses were detected and identified by electron microscopy and polymerase chain reaction (PCR). To determine the genetic characteristics of the Korean strains, the sequence of the major envelope protein (B2L) was determined and compared with published reference sequences. Phylogenetic analysis revealed that the parapoxvirus strains were closely related to not only isolates from Japan, but also isolates from Germany, Sudan and the United states. This is the first report on an outbreak and the molecular characterization of BPSV in Korea.</P>
Oem, Jae-Ku,Chung, Joon-Yee,Byun, Jae-Won,Kim, Ha-Young,Kwak, Dongmi,Jung, Byeong Yeal The Society ; Maruzen Co. [distributor] 2012 The Journal of veterinary medical science Vol.74 No.12
<P>A national serological survey of caprine arthritis-encephalitis virus (CAEV) infection was conducted using an enzyme-linked immunosorbent assay (ELISA) and an agar gel immunodiffusion (AGID) test. A total of 658 black goats of various breeds were sampled from 59 farms in three regions of Korea. The CAEV-positive goats were predominantly detected in the Southern region (n=17) as compared with the Northern (n=1) and Central regions (n=0) (χ(2)=6.26, P=0.044). Among 658 goats tested, 18 were positive in both ELISA and AGID, indicating a CAEV prevalence of 2.73% (95% confidence interval: 1.74-4.28). These results indicate that CAEV is present in Korean black goats.</P>
Novel Kobuvirus species identified from black goat with diarrhea
Oem, J.K.,Lee, M.H.,Lee, K.K.,An, D.J. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.172 No.3
<P>In this study, a caprine kobuvirus was identified from the diarrheal feces of a Korean black goat. The virus had an 8291-nucleotide-long genome excluding the poly(A) tail, and the genome contained a large open reading frame of 7437 bp, which encoded a putative polyprotein precursor of 2479 amino acids. Full-genome sequence analysis indicated that the caprine kobuvirus was most closely related to porcine kobuvirus, with 75.9% amino acid similarity and 70.1% nucleotide similarity. The predicted secondary structure of the 5' terminus (110-bp region) of strain 12Q108 consisted of three stem-loop domains. The first loop sequence of 12Q108 differed from that of strain Y-1-CH1 at one nucleotide position. The full genome substitution rates of caprine kobuvirus were assessed and the time to the most recent common ancestor (TMRCA) of caprine kobuivirus was compared with that of kobuvirus strains from other species. The rate of evolutionary substitution was determined to be 6.40 x 10(-3) substitutions per site per year, and the TMRCA was determined to be 149.6 years for kobuviruses. Furthermore, the caprine kobuvirus TMRCA was determined to be 48.8 years (95% highest posterior density: 8.3-88.4). (C) 2014 Elsevier B.V. All rights reserved.</P>