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N-glucosylation in Corynebacterium glutamicum with YdhE from Bacillus lichenformis
Obed Jackson Amoah,Hue Thi Nguyen,Su Yeong Ma,Jae Kyung Sohng 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
A system was developed to successfully biosynthesize glucoside derivatives of therapeutic and relevant N-linked compounds in the generally regarded as safe (GRAS) Corynebacterium glutamicum. Using glucose, as the sole carbon source, YdhE, which is a promiscuous glycosyltransferase from Bacillus lichenformis and has been introduced in the Corynebacterium glutamicum cell factory system, was able to biosynthesize most of the N-linked compounds to their rare glucosides. Optimal conditions such as IPTG concentrations and induction time, Temperature, Substrate and glucose concentration, and Production time on the In Vivo conversion were successfully developed. This yielded almost 85% of glucosylated butyl-4-aminobenzoate glucoside after only 10 hours post substrate induction. The developed system uses recombinant Corynebacterium glutamicum to achieve In Vivo glucosylation using cheap glucose as the main source of UDP-sugar moieties. Hence making it feasible for industrial-scale biosynthesis of glucoside derivatives by bypassing the demand and use of the more expensive UDP-glucose cofactor that is used in most work that involves glucosylation.
Study of Salting Effect of Inorganic Salts on Nano- and Giant Polymersomes
Obed Andres Solis-Gonzalez,Christopher Chi Wai Tse,Patrick J. Smith,J. Patrick A. Fairclough 한국고분자학회 2022 Macromolecular Research Vol.30 No.9
The salting effect, of kosmotropic and chaotropic salts, on nanoscale and giant-poly(ethylene oxide)16-block-poly(butylene oxide)22 polymersomes was studied. Nanovesicles were studied by dynamic light scattering and transmission electron microscopy and their aggregates (i.e., gel-like structures) by optical microscopy. Giant polymersomes were formed using drop-on-demand inkjet printing and subsequently observed using an optical microscope. The kosmotropic salts were found to strongly influence both vesicle aggregation and giant vesicle (above 3 μm) formation at high salt concentrations (i.e., ~0.5M for nanovesicle aggregation and 0.23 M for giant polymersomes) where the salting effect dominates. Chaotropic salts have very little effect in both systems. The ionic specificity effect essentially determined the outcome of experiments since the type of anion provides different ion/water/ poly(ethylene oxide) interactions and different osmotic stress values, which can be considered the main driving forces and lead the effects observed in experiments (nanovesicle aggregate experiments and abundant giant vesicle formation experiments). The applications of the present work may be lead to better methodologies to produce gel scaffolds for biomaterials or giant unilamellar polymersomes.
Obed Cortés-Aburto,José-Alfredo Hernández-Pérez,Rafael Rojas-Rodríguez 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.8
We performed a performance assessment of a recently developed method: Lévy flight particle swarm optimization. This method, which was developed for optimization problems, has shown good results. There is not an application to inverse problems. Now, it is applied for inverse heat transfer, and results are good enough. In particular, a parameter called “limit” is analyzed for different values (from 10 to 150). It shows that performance of the method for estimating parameters in inverse problems is affected by this parameter. When limit reaches a value of 80, ordinary least squares norm and consuming time begin to exhibit better results.
CV Driven Resyllabification of Personal Names in Ẹdo
Osaigbovo Obed Evbuomwan,Veronica EdamweEdobor-Uzamere 세종대학교 언어연구소 2022 Journal of Universal Language Vol.23 No.2
Nouns in the Ẹdo language have been analysed as obligatorily beginning with a vowel. This paper examines personal names as one instance in which that previously conclusive assumption is violated. The synchronic data analysed in this study show that personal names are freely transformed in such a way that they are used with word initial consonants. The study considers this phenomenon as a resyllabification process that seeks to modify personal names to a CVCV syllable pattern. The study analysed personal names that were collected from Ẹdo speakers across the three local government areas that make up Benin city, the capital of Edo state, Nigeria. The study relies on the principles of the CV-phonology model that began in Clements & Keyser and finds that many Ẹdo personal names are functional with or without the word-initial vowels, a re-syllabified name may become meaningless, have adjusted meaning or retain its original meaning. The paper concludes that resyllabification in multi-syllabic personal names is gradual and the overall goal is to reduce them to CVCV syllable patterns.
Severe acute alcoholic hepatitis and liver transplant: A never-ending mournful story
Aiman Obed,Abdalla Bashir,Steffen Stern,Anwar Jarrad 대한간학회 2018 Clinical and Molecular Hepatology(대한간학회지) Vol.24 No.4
Severe acute alcoholic liver disease (SAAH) unresponsive to medical therapy shows one-year-mortality rates of up to 90%. Most transplant centers request six months of alcohol abstinence prior to transplantation, the so-called “6-month rule.” This regulation is not based on strong evidence, repeatedly making it a topic of controversial debates. The majority of patients with SAAH will die before fulfilling the 6-month rule. Therefore, liver transplantation (LT) protocols are becoming more flexible towards the rigid abstinence regulation, especially concerning SAAH patients. We conducted a literature review regarding LT in SAAH and its outcomes, including post-transplant mortality and recidivism. We studied available data on PubMed from 2011 and onwards whilst including articles dealing with genetic components, medical therapy and historic snapshots of alcoholism. Emerging studies recommend LT in SAAH not responding to medical therapies even without realizing the required abstinence period, since the majority of these patients would die within 6 months. SAAH without response to medical therapy has one-year-mortality rates of up to 90%. The 6-month rule is not based on strong evidence and is repeatedly a topic of controversial debates. There is genetic linkage to alcoholism and medical therapy is not as effective as estimated, yet. The 6-months-regulation has not shown to evidently decrease the risk of recidivism post-LT, which is a lifesaving treatment in SAAH patients. Insisting on rigid sobriety rules results in excluding patients with a low risk of recidivism from being transplanted. Moreover, the genetic linkage of alcoholism must be recognized.
Evolving Business Models to Support the Growth of Technology-based Start-up Firms
Malcolm Parry OBE 세계과학도시연합 2016 World Technopolis Review Vol.5 No.2
There is both a commercial and social imperative for innovation-led economic development. The data on inventions shows the importance of cities in the role of creating this important foundation to innovation. The importance of new venture firms in the innovation process of taking inventions to market is widely recognised, and with this recognition the science parks and science cities are working to understand and replicate the social, business and technology environments which are most fertile for these companies. This paper reviews how those with an interest in this process have addressed this challenge with new business practices and planning activities. It draws particularly on the experiences of Tech City in London and the Surrey Research Park.
Lee M . R obe rts 국제언어인문학회 2014 인문언어 Vol.16 No.1
Following the Reichspogromnacht of 9-10 November 1938, widely known under the Nazi euphemism Kristallnacht, Shanghai witnessed the influx of nearly 20,000 German-speaking Jews. Among these exiles were the Jewish-Austrian writers Hans Schubert and Mark Siegelberg who authored and staged two dramas: Die Masken fallen (The Masks Fall), which played at the British embassy in Shanghai on 9 November 1940, presents an Aryan German man and his Nazi lawyer who pressure an Aryan German woman to divorce her Jewish husband, until finally the couple flees to Shanghai. Fremde Erde (Foreign Soil), performed through EJAS (European Jewish Artist Society) on 8 and 10 April 1941, portrays a Jewish-Austrian couple exiled in Shanghai. The husband was once a successful physician who cannot find enough money to open a new medical practice in Shanghai until his wife sells her body to a wealthy Chinese man. Despite the seemingly countless hardships the exiles face in Shanghai, the greatest impediment to this couple’s recovery turns out to be their own racism, the very force that caused the Nazis to expel them so violently from Europe.
The Surrey Research Park: A Case Study of Strategic Planning for Economic Development
Malcolm Parry,OBE 세계과학도시연합 2012 World Technopolis Review Vol.1 No.3
The development of the Surrey Research Park by the University of Surrey is an addition to a number of existing strategies to collaborate with industry that it has developed over its 120 year history. The potential to undertake this development was based on owning a substantial land holding that the University acquired when the Borough Council for the town of Guildford invited the University to relocate from Battersea in London to its new location in 1966. Initial plans for the Park in 1979 were accelerated in 1981 in response to plans by the then government to reduce funding for Higher Education in the UK. Beyond a broad master plan for the site that was based on topography and access to the site the plans that were developed were based on a survey of 100 companies that were deemed to be in the target market for the site and a review of the other 7 science parks that were being developed in the UK in 1981. The findings from this proved to be important in developing the master plan for the site. Another important influence on the project was the objectives that were defined for the 3 stakeholders in the project of the University, the tenant companies and the planning authority relate to economic development, a competitive advantage of tenant companies, knowledge transfer, the profile for the University and the capacity to generate income proved to be a valuable framework on which to develop a master plan. These details were underpinned by five objectives which served the three stakeholders in the site. Those for the University included commercial potential, knowledge transfer and image and reputation; those for the town primarily related to economic development and the plan was to help tenants gain a competitive advantage by locating on the site. In addition a number of success indicators were defined for the project against which to measure performance and have remained as a useful set of parameters on which to base the assessment of the performance of the site. In combination with these indicators a further analysis deals with the success factors that are considered as important in influencing performance. The paper sets details the history of the park and covers the success indicators and factors and reviews these in the context of the original objectives for the site.