A conserved splicing silencer dynamically regulates O-GlcNAc transferase intron retention and O-GlcNAc homeostasis

Sung-Kyun Park,Xiaorong Zhou,Kathryn E. Pendleton,Olga V. Hunter,Jennifer J. Kohler,Kathryn A. O’Donnell,Nicholas K. Conrad 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01

Modification of nucleocytoplasmic proteins with O-GlcNAc regulates a wide variety of cellular processes and has been linked to human diseases. The enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) add and remove O-GlcNAc, but the mechanisms regulating their expression remain unclear. Here, we demonstrate that retention of the fourth intron of OGT is regulated in response to O-GlcNAc levels. We further define a conserved intronic splicing silencer (ISS) that is necessary for OGT intron retention. Deletion of the ISS in colon cancer cells leads to increases in OGT, but O-GlcNAc homeostasis is maintained by concomitant increases in OGA protein. However, the ISS-deleted cells are hypersensitive to OGA inhibition in culture and in soft agar. Moreover, growth of xenograft tumors from ISS-deleted cells is compromised in mice treated with an OGA inhibitor. Thus, ISS-mediated regulation of OGT intron retention is a key component in OGT expression and maintaining O-GlcNAc homeostasis.

광탄성실험 Hybrid 법에 의한 일정한 변형율을 받는 O-Ring 의 응력해석

권오성(O-Sung Kwon),황재석(Jai-Sug Hawong),남정환(Jeong-Hwan Nam),한송령(Song-Ling Han),박성한(Sung-Han Park) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.11

In this research, stresses of O-ring with uniform deformation ratio in upper and lower direction are analyzed by photoelastic experimental hybrid method. The O-ring is made from rubber and is used to sealing of the high pressure vessel. The compressive deformation ratios of O-ring are 10% and 20%. Stress components (σ<SUB>xx</SUB>, σ<SUB>yy</SUB>, τ<SUB>xy</SUB>) of O-ring uniform ratios obtained from the photoelastic experimental hybrid method are almost identical to those from Hertz’s theory. Therefore, stress freezing method and photoelastic experimental hybrid method are effective applied to the stress analysis of O-ring made from rubber and with uniform ratios. The loading device developed in this research is used to produce the uniform deformation ratio of O-ring and as loading device of stress freezing. Stress distributions results from the upper contact surface of O-ring are almost identical to those from the lower contact surface of O-ring without regard to squeeze deformation ratio.

Effect of Network Polymerization on the Pressure-Induced Structural Changes in Sodium Aluminosilicate Glasses and Melts: ²⁷Al and ¹⁷O Solid-State NMR Study

Lee, Sung Keun,Yi, Yoo Soo,Cody, George D.,Mibe, Kenji,Fei, Yingwei,Mysen, Bjorn O. American Chemical Society 2012 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.116 No.3

<P>Probing the pressure-induced structural changes and the extent of disorder in aluminosilicate glasses and melts at high pressure remains a challenge in modern physical and chemical sciences. With an aim of establishing a systematic relationship between pressure, composition, and glass structures, we report <SUP>27</SUP>Al and <SUP>17</SUP>O 3QMAS NMR spectra for sodium aluminosilicate glasses [Na<SUB>2</SUB>O:Al<SUB>2</SUB>O<SUB>3</SUB>:SiO<SUB>2</SUB> = 1.5:0.5:2<I>n</I> with <I>n</I> = 1 (NAS150520, <I>X</I><SUB>SiO2</SUB> = 0.5), 2 (NAS150540, <I>X</I><SUB>SiO2</SUB> = 0.67), and 3 (NAS150560, <I>X</I><SUB>SiO2</SUB> = 0.75)], quenched from melts at pressures up to 8 GPa. We also explore the stability of the <SUP>[4]</SUP>Al–O–<SUP>[4]</SUP>Al cluster in the highly depolymerized, NAS150520, glass at high pressure. For given glass composition, the <SUP>[5,6]</SUP>Al peak intensity increases with increasing pressure. The population of <SUP>[5,6]</SUP>Al increases linearly with <I>X</I><SUB>SiO2</SUB> from NAS150520 (<I>X</I><SUB>SiO2</SUB> = 0.5) to NAS150560 glass (<I>X</I><SUB>SiO2</SUB> = 0.75) at both 6 and 8 GPa. The <SUP>[5,6]</SUP>Al/<I>X</I><SUB>SiO2</SUB> ratio also tends to increase with pressure, indicating a possible relationship between <SUP>[5,6]</SUP>Al fraction and <I>X</I><SUB>SiO2</SUB> that depends on pressure. The effect of pressure on the network connectivity in the sodium aluminosilicate glasses is manifested in the increase in <SUP>[4]</SUP>Si–O–<SUP>[5,6]</SUP>Al peak intensity and the decrease in the nonbridging oxygen (NBO) fraction with increasing pressure. The fraction of <SUP>[4]</SUP>Si–O–<SUP>[5,6]</SUP>Al in NAS150520 is smaller than in NAS150560. Taking into consideration the pressure-induced Al coordination transformation in the fully polymerized glass (albite, Na<SUB>2</SUB>O:Al<SUB>2</SUB>O<SUB>3</SUB>:SiO<SUB>2</SUB>= 1:1:6, NBO/T = 0), the fraction of <SUP>[5,6]</SUP>Al at a given pressure varies <I>nonlinearly</I> with variations of NBO/T. <SUP>[5,6]</SUP>Al fraction at 8 GPa increases with decreasing degree of melt polymerization from ∼8% for fully polymerized albite melt (NBO/T = 0) to ∼37% for partially depolymerized melt (NAS150560, at NBO/T = 0.29). Then it gradually decreases to ∼15% for NAS150520 with further increase in NBO/T of 0.67. This observed trend in the densification behavior at a given pressure indicates competing densification mechanisms involving steric hindrance vs changes of NBO fraction in the silicate melts. The NMR results also suggest that both NBO and BO, particularly <SUP>[4]</SUP>Si–O–<SUP>[4]</SUP>Si, interact with Na<SUP>+</SUP>, and thus the Na<SUP>+</SUP> distribution is likely to be homogeneous around both NBO and BO at high pressure without spatial segregation of silica-rich and alkali-rich domains for the glass compositions studied here. The presence of the <SUP>[4]</SUP>Al–O–<SUP>[4]</SUP>Al cluster is distinct in the NMR spectrum for NAS150520 glass at both 6 and 8 GPa. A new scheme of pressure-induced structural transitions in silicate melts involving <SUP>[4]</SUP>Al–O–<SUP>[4]</SUP>Al includes the formation of <SUP>[4]</SUP>Al–O–<SUP>[5]</SUP>Al.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2012/jpccck.2012.116.issue-3/jp206765s/production/images/medium/jp-2011-06765s_0008.gif'></P>

장기 저장연료의 열안정성 및 연료접촉 고무오링의 수명예측 연구

정근우(K. W. Chung),홍진숙(J. S. Hong),김영운(Y. W. Kim),한정식(J. S. Han),정병훈(B. H. Jeong),권태수(T. S. Kwon),서동욱(D. O. Suh),성민준(M. J. Sung),권영일(Y. I. Kwon) 한국트라이볼로지학회 2018 한국윤활학회지(윤활학회지) Vol.34 No.5

Thermal deterioration of fuel due to long-term storage influences engine performance and causes malfunctions. Fuel stability is usually evaluated via heat resistance and thermal stability during a brief heat shock at high temperature; storage stability in this scenario means that there is very little change in the quality of the fuel during long-term storage. In addition, rubber-based products such as oil seals, O-rings, and rubber hoses can influence the quality of the fuel. When these rubber products are in contact with fuel, they can swell, mechanically weaken, and occasionally crack, thus leaking low molar weight rubber and additives including plasticizer and antioxidant into the fuel to degrade its properties and shorten its useful lifetime. This study determines the thermal stabilities of three kinds of synthetic fuels by evaluating their low temperature kinematic viscosities, chemical composition changes via GC analyses, gross heat of combustion, and color changes. We evaluate the compression set of O-rings by immersing one NBR and two FKM rubber O-rings in the three synthetic fuel samples in airtight containers at variable storage temperatures for six months; from this, we estimate the lifetimes of the O-rings using the Power law model. There were very little changes in the chemical compositions and gross heat of combustion after six months of the experiment. The lifetimes are thus dependent on the materials of the rubber products, and in particular, the FKM O-ring was calculated to have a theoretical lifetime of 200 to 5,700 years. These results indicate that the synthetic fuels maintain their physical properties even after long-term storage at high temperatures, and the FKM O-ring is suitable for long-term sealing of these fuels.

CHF₃ / C₂F6 플라즈마에 의한 실리콘 표면 잔류막의 특성

권광호(K.-H. Kwon),박형호(H.-H. Park),이수민(S. M. Lee),강성준(S. J. Kang),권오준(O.-J. Kwon),김보우(B.W. Kim),성영권(Y.-K. Sung) 한국진공학회(ASCT) 1992 Applied Science and Convergence Technology Vol.1 No.1

실리콘을 CHF₃/C₂F_6 가스 플라즈마를 이용하여 식각하면 실리콘위에 탄소, 불소 및 산소로 이루어진 잔류막이 형성된다. 이 잔류막을 XPS로 분석한 결과 탄소는 C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, C-F₃ 결합을 하고 있으며, 불소는 F-Si, F-C 및 F-O 결합으로 이루어져 있음을 알았다. 한편 산소는 O-Si 및 O-F 결합으로, 실리콘은 Si-Si, Si-C 및 Si-O 결합상태를 나타낸다. 잔류막의 수직분포 연구를 통하여 Si-O 및 Si-C 결합이 탄소와 불소의 결합층 아래에 존재하고, 잔류막의 표면부에 F-O 결합이 분포함을 알았다. 또한 건식식각 변수가 잔류막 형성에 미치는 영향이 조사되었으며 CHF₃/C₂F_6 가스 유량비, RF power 벚 압력 등이 잔류막의 두께, 조성비 및 잔류막의 결합상태에 영향을 미침을 알 수 있었다. Si surfaces exposed to CHF₃/C₂F_6 gas plasmas in reactive ion etching (RIE) have been characterized by X-ray photoelectron spectroscopy (XPS). CHF₃/C₂F_6 gas plasma exposure of Si surface leads to the deposition of residual film containing carbon and fluorine. The narrow scan spectra of C 1s show various bonding states of carbon as C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, and C-F₃. The chemical bonding states of fluorine are described with F-Si, F-C, and F-O. And the oxygen and silicon are also detected. The effects of parameters for reactive ion etching as CHF₃/C₂F_6 gas ratio, RF power, and pressure are investigated.

방사선조사가 백서 타액선의 clusterin 발현에 미치는 영향

오규명,최용석,황의환,이상래 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate clusterin expression in the acini and ductal cells of rat submandibular glands after Co-60 gamma irradiation. Materials and Methods : The male Sprague-Dawley rats weighing approximately 250 gm were divided into control and experimental groups. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. All the rats were sacrificed at 1, 3, 7, 14, 21, and 28 days after irradiation. The specimens including the submandibular gland were sectioned and observed using a immunohistochemical method. Results : In the 2 Gy group, clusterin expression was similar to that of the control group at 1 day after irradiation and it was observed in the striated ductal cells at 3 days after irradiation. In the 5 Gy group, clusterin expression was observed in the striated ductal cells at 1 day after irradiation and gradually increased in the 10 and 15 Gy groups. In the 15 Gy group, clusterin expression was prominent in the striated ductal cells at 1 day after irradiation, but it gradually decreased with the experimental period. The destruction of the striated ductal cells was observed in the 2 Gy group at 21 days after irradiation and in the 5, 10, and 15 Gy groups at 7 days after irradiation. The destruction of the acinar cells was observed in the 2 Gy group at 28 days after irradiation and in the 5, 10, and 15 Gy groups at 14 days after irradiation. Conclusion : Clusterin expression was induced by low doses of irradiation and it appeared to be involved in the regulation of cellular response to irradiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 33-40)

XML 프로토콜을 지원하는 웹기반 SPC 시스템

오경제,서양진,김성일,한상용 중앙대학교정보통신연구소 2000 정보통신연구소논문집 Vol.2 No.1

경쟁적인 시장에서 살아남기 위해서는 공장의 공정제어를 정확하게 하는 것이 필수적이다. SPC(Statistical Process Control) 시스템을 품질 향상의 욕구를 충족시키기 위해 광범위하게 운영되며, 다른 툴과의 접목에 어려움이 있다. 본 논문이 제안하는 SPC 시스템은 웹을 기반으로 하고, XML을 입력/출력 프로토콜로 이용한다. 또한 강력한 그래픽 표현기능과 효율적인 파일 시스템으로 실시간 제어를 가능하게 한다. 새로운 SPC 시스템은 어떤생산체제에도 사용 가능하지만, 특히 반도체, TFT/LCD 산업에 최적화되어 있다. 시스템의 구현은 C++과 COM/DCOM을 사용하며, 기존의 제품보다 향상된 성능을 보인다. Accurate process control in the manufacturing industry is essential to survive in the competitive market. Statistical process control(SPC) system has been widely used to satisfy the ever-increasing quality control requirements. However, most commercial products in the market are not flexible, semi-automatic, and difficult to interface with other tools. In this paper, we propose an advanced SPC system which is based on the web and supports XML protocol. We also provide a powerful graphic facility and an efficient file system to handle the data in real time. Even though the idea can be applied to any manufacturing system, our system is optimized to the semi-conductor industry and TFT/LCD industry. The system is implemented in C++ and COM/DCOM, and shows a good result.

개혁개방과 중국 조선족 소설문학 : 90년대 상반기 소설문학을 중심으로

오상순 효성여자대학교 한국전통문화연구소 1997 韓國傳統文化硏究 Vol.12 No.-

홀로그램 회절격자의 이동에 의한 포토폴리며의 위상 변위측정

성기영,경천수,김휘성,이진국,곽종훈,최옥식 嶺南大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.18 No.-

Holographic recording and erasure kinetics in a home-made photopolymer film are investigated as a function of monomer concentration and spatial frequency. We also measured phase shifts of index grating and absorption grating by moving holographic photopolymer gratings along a direction of grating wave vector.

Photopolymer의 홀로그램 기록특성

성기영,최옥식,경천수,곽종훈 嶺南大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.17 No.-

We fabricated photopolymer hologram and explained hologram formation processes. Dynamic self-diffraction oscillation of photopolymer is observed by two wave mixing experiment and it is well explained by the modified Kogelnik's coupled wave equation.