Suicide cancer gene therapy using pore-forming toxin, streptolysin O.

Yang, Wan Seok,Park, Sue-O,Yoon, A-Rum,Yoo, Ji Young,Kim, Min Kyung,Yun, Chae-Ok,Kim, Chul-Woo American Association for Cancer Research, Inc 2006 Molecular Cancer Therapeutics Vol.5 No.6

<P>We cloned the streptolysin O gene from the Streptococcus pyogenes genome and tested the possibility of using it as an anticancer reagent. Transient transfection of the streptolysin O gene efficiently killed 293T cells after 12 hours of transfection as determined by lactate dehydrogenase release and propidium iodide uptake. No caspase activity was observed and necrosis was prominent during streptolysin O-induced cell death. Biochemical analysis of streptolysin O protein revealed that the deletion of only 5 amino acids from the COOH-terminal region of streptolysin O, which is essential for cholesterol binding activity, abolished its cell-killing activity, whereas the NH2-terminal region was more resilient, i.e., up to 115 amino acids could be deleted without changing its cell-killing activity. We generated a streptolysin O-expressing adenovirus and injected it into human cervical cancer cell-derived tumors grown in a nude mouse model. Twenty-one days postinjection, the average size of tumors in the streptolysin O adenovirus-injected group was 29.3% of that of the control PBS-treated group. Our results show that the genes of pore-forming toxins, like streptolysin O protein, have the potential to establish a novel class of suicide gene therapeutic reagents.</P>

디젤 엔진 Urea-SCR DeNOx 시스템용 혼합전위 방식 암모니아 가스 센서의 감지물질 특성 비교

최안기 ( An Gi Choi ),양영창 ( Young Chang Yang ),구본철 ( Bon Chul Koo ),박종욱 ( C. O. Park ) 한국센서학회 2010 센서학회지 Vol.19 No.3

It is considered that the urea injection DeNOx SCR(selective catalytic reduction) system is the only promising method to satisfy the worldwide NOx emission standards. As for the theoretical aspect, reactants of NO and NO2 with NH3 produce H2O, N2 and O2 which do not harm human beings and environmental as well. The realization of maximum NOx conversion (without using a post oxidation catalyst) is only possible with closed loop controlled urea dosing. It means built-in NH3 gas sensor have to be developed for detecting accurate NH3 concentration for the feedback system. Using YSZ(yttriastabilized zirconia) as a solid state electrolyte and In2O3 as a sensing material, this paper aims to study dependable NH3 gas sensor for the promising solution of DeNOx technology, which have a reproducible electric output signal, a high sensitivity and fast response.

매립이 진행중인 일반폐기물 매립지에 있어서 침출수의 성상

이재오,서정인,이철희,권대원 嶺南大學校 環境問題硏究所 1995 環境硏究 Vol.14 No.2

매립지에 있어서 매립층의 폐기물의 분해상황을 평가하기 위하여, 매립이 진행중인 매립지의 침출수의 pH, 유기물질, 질소화합물, 중금속농도의 변화를 3년간 조사하였다. 그 결과 pH는 6.7 ~ 9의 범위로 나타났으며 매립기간의 경과와 함께 증가하는 경향을 보였다. BOD 및 COD의 농도범위는 각각 1,800 ~ 25,000 mg·???, 1,700 ~ 6,000mg·???로 나타났으며, 그 유출농도는 하절기에는 증가하고 동절기에는 감소하는 경향을 보였다. T-N의 농도범위는 1,000 ~ 4,000mg·???이었으며, 매립량의 증대와 함께 그 농도는 지속적으로 증가하였으며, 질소화합물의 대부분은 ?????????의 형태로 존재하였다. 한편, T-P의 평균농도는 20mg·???였다. SS의 농도는 150 ~ 600mg·??? 이었으며, 중금속의 유출농도는 매립기간의 경과와 함께 감소하는 경향을 보였다. 이와 같은 결과로부터 본 매립지의 매립폐기물의 분해는 혐기적상태에서 주로 이루어지고 있으며, 강우에 의한 미분해성 유기물의 세출현상이 활발한 것으로 추측되었다. The purpose of this research was to evaluate the decomposition properties of refuse by analysis of landfill leachate. The leachate was analyzed in terms of pH, organic and inorganic compounds, and heavy metals for 3 years. The results are as follows; The pH of leachate ranged from 6.7 to 9. BOD and COD were measured in the range of 1,800 ~ 25,000 mg·??? and 1,700 ~ 6,000 mg·???, respectively. It showed that the seasonal variation of BOD and COD appeared to increase in summer season, to decrease with decreasing temperature. Concentration of T-N was in the range of 1,000 ~ 4,000mg·???, it increased with augment of landfill volume, and the greater part of T-N existed form of ammonium nitrogen. The SS was observed in the range of 150~600 mg·??? and the average concentration of T-P was 20 mg·???. Also, concentration of heavy metals showed that it is tend to decrease in processing phase. From these results, it indicated that refuse of this landfill was decomposed in anaerobic condition, actively. Concentration of organic compounds increased in summer season. It results in the physical washout of organic compounds by rainfall and the extension of microbial decomposition activity inside landfill.

완전탈구된 치아의 재식에 관한 치험례

최영철,권오연,김광철,이긍호 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

Exarticulation includes all cases in which the tooth has been totally displaced out of its socket. The main etilogies in the permanent dentition are direct blows and traffic accident, while in the primary dentition are fall-downs. The prognosis of tooth which has been recieved endodontic treatment after replantation is better than received endodontic treatment before replantation. Ca(OH)₂as a initial canal filling material diminishes undesirable replacement root resorption, so in the cases of compelted and uncompleted root formation, the pulp is extirpated and then a Ca(OH)₂dressing is placed until optimal stage for final canal filling with gutta percha cones. Storage media and storage period of avulsed tooth is very important things for prognosis after replantation. A strong relationship has been found between dry storage or storage in non physiologic media (c.g tapwater) and root resorption. Physiologic saline and/or fresh milk is one of the best storage media.

프로야구 손상의 임상적 분석

김철준,김명화,김미정,신명진,윤준오,김상규 대한스포츠의학회 1995 대한스포츠의학회지 Vol.13 No.1

Baseball is rapidly getting popularity in Korea as it has been in U.S.A and Japan. During the last decade, threre were several studies about baseball injuries which oriented mainly to the overuse injuries of growing baseball players. But there was no study the professional baseball players systematically. This study was surposed to see the general characteristics of baseball injuries and to provide the prevention and rehabilitation programs if recommended. The number of 83 injuries of 60 baseball players was analysed according to the position, injury site, diagnosis, injury mechanism and duration of injury. The mean age and height of 60 subjects were 24.6 years old and 180.65㎝, respectively. The baseball position of 82 injured cases were composed of 27 pitchers, 30 infielder, 19 outfielder and 6 catchers. The characteristics of injury were as follows: 1.The number of chronic injury was 56 cases(68.3%) and the percentage of chronic injury of pitchers was up to 81.5% compared 73.3% of infielder and 47.4% of outfielder. 2. The injury rate of both lower and upper extremitis were equal and the rate of lower back problems was the second, which should be concerned. 3. The number of cases injured by direct collison is as low as 15(18.3%). The most common secondary cause of injury was aggressive throwing itself and 26 out of 49 cases injured not by direct collision. 4. More concern should be payed about the low back problems and about the catcher and the field players as much as pitchers from now one.

갑상선 종양에서 thrombin receptor (PAR-1)의 발현 양상

박지영,목지오,김여주,박형규,김철희,변동원,서교일,유명희,조현득,오미혜,김상진 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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PCR을 이용한 국내 및 수입 축산물 유래 Listeria monocytogenes의 유전학적 분석연구

우용구,이수화,이철현,최정수,류재두,김영일,이오수,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

One hundred-fifty two of Listeria strains were isolated from domestic chicken carcasses, a slaughter-houses from nationwide from 1996 to 1997 and imported livestock products from a ten foreign countries including the USA, China, France and Thailand. To substitute the laborious and time consuming procedures of clinical laboratory standard diagnostic methods, the rapid and specific multiplex PCR(M-PCR), which was designed to amplify a three kinds of genes simultaneously f3r specific detection and differentiation of L. monocytogenes(LM) by one step procedure, was established. The LM strains confirmed with M-PCR were analysed to their genetic diversities by RAPD using the two primers(D87 & MMTl), and also evaluated on their discriminatory abilities(na) between two primers. our study also conducted the REP-PCR and ERIC-PCR for evaluation of the possibility of REP and ERIC elements for genetic subtyping of LM strains. According to the REP-PCR and ERIC-PCR fingerprinting patterns, LM strains were divided into 7(ERIC-type) and 4(REP-type) major clusters at the relative clone cut off value of 80%. According to the computer analysis of PCR results using the GelCompar Ⅱ software, ERIC-PCR(DI=0.955) had expressed the most high discriminatory ability and followed by REP-PCR(DI=0.952), RAPD(D87; 0.954, MMT1; 0.937), in order. The combined analysis of the present PCR results was also expressed the reliable and useful discriminatory ability(DI=0.916). This study suggested that PCR based methods could be used as an reliable, reproducible, rapid, and highly discriminatory method for the genetic differentiation of genus Listeria.

고유함수전개법을 이용한 비대칭 유전체 원주공진기의 공진특성해석

류원열,민경호,장중호,전오곤,이창화,최현철 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

The resonant frequency of the dielectric rod resonator which is surrounded by cylindrical cavity can be calculated very exactly by using eigenfunction expansion method in the concept of electric and magnetic wall from the symmetry. But if the supporter is put in the cavity, the symmetry disappears. Thus this method has some errors. In this paper, the resonant frequency of the asymmetric dielectric rod resonator was calculated by using the eigenfunction expansion method. Also, the effect of the supporter was discussed. Then we found that the resonant frequency was more affected in the case of TM mode than TE or Hybrid modes as the length of the supporter was longer.

Terahertz Phonon Modes of Highly Efficient Electro-optic Phenyltriene OH1 Crystals

Kim, Jongtaek,Lee, Seung-Heon,Lee, Seung-Chul,Jazbinsek, Mojca,Miyamoto, Katsuhiko,Omatsu, Takashige,Lee, Yoon Sup,Kwon, O-Pil American Chemical Society 2016 The Journal of Physical Chemistry Part C Vol.120 No.42

<P>Understanding the origin of the phonon modes of highly efficient electro-optic crystals is very important for designing materials and for optimizing their photonic applications. Here we investigate the origin of phonon modes in the 0.1-15 THz range of the benchmark electrooptic OH1 (2-(3-(4-hydroxystyry1)-5,5-dimethylcyclohex-2-enylidene)malononitrile) crystal, which is interesting due to its large electro-optic coefficient and high THz-wave generation efficiency. The phonon modes (and vibrational absorption properties) of OH1 crystals are evaluated theoretically by periodic density functional theory and also experimentally by THz absorption spectroscopy. The the oretical calculations are well-matched with experimental results. The THz absorption properties are highly anisotropic; the amplitude of the vibrational absorption is the largest along the polar c-axis compared to the other two crystallographic axes. For comparison, the vibrational absorption modes of the OH1 molecule in the gas phase are also calculated. The calculated vibrational absorption spectrum of OH1 crystalline powder appears similar to that of the OH1 molecule in the gas phase. However, the molecular vibrational motions in the crystalline state are coupled motions of vibrational motions in the gas phase. Interestingly, the vibrational mode of the torsion of the O-H bond with the largest absorption strength in the gas phase is in the crystal inhibited due to the crystal field effect. The origin of the intense phonon modes of OH1 crystals is mainly related to relatively strong distortions of the push pull pi-conjugated system including electron donor and acceptor groups.</P>

濟州道産業開發計劃 및 空港整備計劃의 一體化에 관한 硏究

柳光井,宋喜鐵,朴五華,咸永勳 한국항공대학교 1978 論文集 Vol.13 No.-

Cheju Island is evaluated as a best area for four seasons tourist site with it's rich natural and cultural resources. The study team aimed to suggest a course of action to utilize the retained resourses as fully, and the effective way of positive drive for the Cheju Island development plan. as a conclusion, the study team suggest to establish Cheju Island development organization as a corporate body, and the newly established organization should consolidate the development paln for resouces and geographical postion as a merit, and airport developament plan to compensate the defect of transportation view point.