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최효근(Hyo Geun Choi),김윤중(Yoon joong Kim),박보나(Po na Park),홍승노(Seung No Hong),성명훈(Myung Whun Sung),하정훈(J Hun Hah) 대한두경부종양학회 2012 대한두경부 종양학회지 Vol.28 No.2
Introduction : No drain technique during parotidectomy had been introduced to reduce postoperative morbidity and to minimize hospital stay in a few previous publications. Since the authors have applied this technique in select patients for several years, we wanted to evaluate the feasibility and usefulness of no drain parotidectomy. Material & Methods : We retrospectively reviewed the medical records of 96 patients who underwent superficial or total parotidectomy by one surgeon from May 2005 to July 2012. The decision on drain insertion was made by the operator at the end of the surgery. The patients were categorized as drain insertion group and no drain group. Results : The patients who have smaller tumors and benign lesions were more frequently chosen into no drain group. Hospital stay was shorter in no drain group than in drain insertion group. Although no drain group showed increased number of acute complications such as seroma and hematoma, the complications were mild and could be controlled easily at the outpatient clinic. Conclusion : No drain technique during parotidectomy could be done relatively safely in select patients and it could reduce hospital stay.
No-Joong Park,Sae-Chan Oh,Yong-Ho Choi,Kwang-Ryeul Choi,Kwang Yun Cho 한국응용곤충학회 2004 Journal of Asia-Pacific Entomology Vol.7 No.2
The inheritance of the phenthoate-selected diamondback moth (DBM) was investigated, and the cross resistance was also examined against various insecticides. The phenthoate concentration-mortality relationship between F1 progenies (S♀×R♂, S♂×R♀) obtained from reciprocal crosses with the susceptible (S) and phenthoate-selected DBM strains (R) indicated the absence of sex-linked inheritance. The resistance ratios of F1S (S♀×R♂), F1R (S♂×R♀), and phenthoate-selected(R) strains were 23.8, 24.1, and 175.1, respectively. Degrees of dominance of the F1S and F1R progenies were 0.23 and 0.45. These results suggest that the inheritance of phenthoate resistance in DBM is controlled by one or more autosomal genes in a manner of incomplete dominance. This R strain exhibited a high level of cross resistance to parathion, pyrachlofos, and phosmet with resistance ratios of 51.1, 59.9 and 72.5, respectively, and also showed medium level of cross resistance to most organophosphorus insecticides with the range of resistance ratios among 5.0~50.0, except for acephate, demeton-S-methyl, profenofos, and pyridaphenthion showing low level of cross resistance with resistance ratios among 1.5-4.0. However, this R strain exhibited low level of cross resistance to most carbamate insecticides except BPMC showing resistance ratio of 5.3. Similar low level of cross resistance was observed from the pyrethroid and neonicotinoid insecticides with the resistance ratios among 1.2~4.6.
Lingocellulose degrading activity in digestive tract of Trypoxylus [Allomyrina] dichotoma
No-Joong Park,Hee Kyung Lim,In Taek Hwang 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10
The larvae of Asian rhinoceros beetle, Trypoxylus [Allomyrina] dichotoma (Coleoptera: Scarabaeidae) feed on decaying organic matter and can be found in compost heaps or old dead logs. Mass rearing of this insect with a sawdust-based diet has made it popular commercial pet in many countries of Asia. Recently, lignocellulose, or biomass has been focused as renewable and sustainable resources for energy and many chemical products. It is well-known that gut symbiotic microbes in consortia are inevitable for the lignocellulose digestion in many insects. The cellulase and xylanase activity of the crude extracts from each gut contents and tissues, feces, and blood as a control from Asian rhinoceros beetle were analyzed with the overlaid agar-plate assay and spectrophotometric reduced sugar determination. In the plate assay, the highest activity of cellulase and xylanase was observed from midgut tissue extracts, and next from midgut contents, and minor activity from feces. Whereas no enzyme activity was detected from the samples from blood, crop, and hind gut. Similar results were confirmed from the reducing sugar assay with substrates of Avicell and xylan from birchwood, respectively. Further research on the biochemical and genetic property of symbiotic microbes and their enzymes relating on the lignocellulose degradation is planned.
No-Joong Park,Sae-Chan Oh,Yong-Ho Choi,Kwang-Ryeul Choi,Kwang-Yun Cho 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.1
The Inhibition of AChE activity was compared between susceptible and phenthoate resistant diamondback moth (DBM) using in vitro enzyme assay and native polyacrylamide gel electrophoresis (PAGE). Dichlorvos and carbofuran were very effective to inhibit the AChE activity from S strains with I50 values of 0.38 and 0.21 M, respectively. However, the AChE from R strain was much less inhibited to dichlorvos, i.e. insensitive to dichlorvos with R/S ratio of 11.4, than phosphamidon, BPMC, and carbofuran with the R/S ratio of 2.8, 4.2, and 2.5, respectively. Direct-staining of native gel revealed that the majority of AChE from S strain was quickly deactivated by 50 M of dichlorvos that was the lowest concentration of the inhibitor in the experiment. However, the AChE activity from R strain was not fully inhibited by 500 M of dichlorvos. This result was confirmed in the repetitive experiment with head extracts of both strains separately prepared with three replications. In the incubation of AChE with 100 M of dichlorvos on native gel, the AChE from R strain was apparently insensitive to the inhibitor in contrast to the fully inhibited AChE from S strain.
No-Joong Park,Hee Kyung Lim,In Taek Hwang 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.3
Trehalase (EC 3.2.1.28) hydrolyses the non-reducing disaccharide trehalose, the main source of glucose in insects, nematodes, and fungi, into 2 glucose molecules. Therefore, development of specific and potent trehalase inhibitors as control agents against insect pests and other deleterious organisms has been of great interest. For the establishment of a 96-well microplate assay to detect trehalase activity, the o-toluidine method of glucose determination was deliberately modified with whole body extracts from housefly (Musca domestica Linnaeus) larvae serving as an enzyme source. In the microplate trehalase assay, optical density (OD) at 630 nm increased proportionally with acetic acid concentration up to 90%, with the OD rate of increase greatest at 10% o-toluidine. Sensitivity in a 96-well plate reaction was slightly better than with spectrophotometric measurements, especially at low concentrations of glucose. Samples from 70% ammonium sulphate precipitation showed the greatest activity with minimum noise caused by endogenous reducing sugars and a maximum enzymatic activity at pH 4.5 and 40 °C, with substrate inhibition at trehalose concentrations greater than 320.0 mM. The I50 values of the 2 trehalase inhibitors tested, amygdalin and validamycin A, were 31.7 mM and 0.4 mM, respectively. In conclusion, this 96-well microplate assay of trehalase activity using the whole body extract from housefly larvae as an enzyme source might be useful in discovery of potent trehalase inhibitors through the development of new insecticides from screening numerous synthetic and natural compounds. Trehalase (EC 3.2.1.28) hydrolyses the non-reducing disaccharide trehalose, the main source of glucose in insects, nematodes, and fungi, into 2 glucose molecules. Therefore, development of specific and potent trehalase inhibitors as control agents against insect pests and other deleterious organisms has been of great interest. For the establishment of a 96-well microplate assay to detect trehalase activity, the o-toluidine method of glucose determination was deliberately modified with whole body extracts from housefly (Musca domestica Linnaeus) larvae serving as an enzyme source. In the microplate trehalase assay, optical density (OD) at 630 nm increased proportionally with acetic acid concentration up to 90%, with the OD rate of increase greatest at 10% o-toluidine. Sensitivity in a 96-well plate reaction was slightly better than with spectrophotometric measurements, especially at low concentrations of glucose. Samples from 70% ammonium sulphate precipitation showed the greatest activity with minimum noise caused by endogenous reducing sugars and a maximum enzymatic activity at pH 4.5 and 40 °C, with substrate inhibition at trehalose concentrations greater than 320.0 mM. The I50 values of the 2 trehalase inhibitors tested, amygdalin and validamycin A, were 31.7 mM and 0.4 mM, respectively. In conclusion, this 96-well microplate assay of trehalase activity using the whole body extract from housefly larvae as an enzyme source might be useful in discovery of potent trehalase inhibitors through the development of new insecticides from screening numerous synthetic and natural compounds.
Park No-Joong,Oh Sae-Chan,Choi Yong-Ho,Choi Kwang-Ryeul,Cho Kwang-Yun Korean Society of Applied Entomology 2005 Journal of Asia-Pacific Entomology Vol.8 No.1
The Inhibition of AChE activity was compared between susceptible and phenthoate resistant diamondback moth (DBM) using in vitro enzyme assay and native polyacrylamide gel electrophoresis (PAGE). Dichlorvos and carbofuran were very effective to inhibit the AChE activity from S strains with $I_{50}$ values of 0.38 and $0.21\;{\mu}M$, respectively. However, the AChE from R strain was much less inhibited to dichlorvos, i.e. insensitive to dichlorvos with R/S ratio of 11.4, than phosphamidon, BPMC, and carbofuran with the R/S ratio of 2.8, 4.2, and 2.5, respectively. Direct-staining of native gel revealed that the majority of AChE from S strain was quickly deactivated by $50\;{\mu}M$ of dichlorvos that was the lowest concentration of the inhibitor in the experiment. However, the AChE activity from R strain was not fully inhibited by $500\;{\mu}M$ of dichlorvos. This result was confirmed in the repetitive experiment with head extracts of both strains separately prepared with three replications. In the incubation of AChE with $100\;{\mu}M$ of dichlorvos on native gel, the AChE from R strain was apparently insensitive to the inhibitor in contrast to the fully inhibited AChE from S strain.