[Book Review] A birth that changed a nation : A new model of care and inclusion

Nisha Singh Asian Center for Women's Studies : Ewha Womans Uni 2018 Asian Journal of Women's Studies(AJWS) Vol.24 No.2

Tunable Release of Combined Contraceptive Steroids from Coreshell Gelatin/PCL Fibers

Nisha U,Merline C,Lakshminarayanan Ragupathy,Diksha Painuly 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

The present study investigates simultaneous release of two hydrophobic contraceptive steroids from core-shellfibers made by coaxial electrospinning. The contraceptive steroids levonorgestrel (LNG) and ethinylestradiol (EE) wereincorporated in gelatin/poly(ε-caprolactone) (PCL) core-shell fibers. The influence of shell concentration and core feed/flow rate (ml/h) on the physical, chemical, mechanical and release properties of drug incorporated coaxial fibers wereevaluated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), differential scanningcalorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, universal testing machine (UTM) and highperformanceliquid chromatography (HPLC). SEM results revealed the influence of feed/flow rate on pore size (in the range586-1036 nm) and fiber diameter (i.e. 621-1650 nm) of the coaxial fibers. TEM analysis confirmed the presence of coreshellmorphology. DSC results conferred that drugs were in an amorphous form within core-shell fibers. The FT-IR spectraestablished the drug encapsulation by the electrospinning process. Swelling studies demonstrate that increasing the shell(PCL) concentration i.e. 4-10 % w/v decreases the swelling ratio (295-140 %). The drugs release kinetics satisfactorilydescribed by first-order (R2>0.95) model and Korsmeyer-Peppas model (R2≥0.95) for all the prepared core-shellformulations. These formulations were found to follow anomalous non-Fickian transport, which suggests that the drugrelease is controlled by both diffusion and erosion of polymer matrix. These results clearly demonstrate that it is possible tocontrol the release rate for the two hydrophobic (contraceptive) drugs through coaxial electrospinning process for the firsttime to the best of our knowledge.

Synergistic Multi Enzyme Platform for Isopropanol Detection Using Electrochemical Biosensing Assay

Nisha BHARDWAJ,Myeong-Eun LEE,Young Jin KO,Sung Ok HAN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The electrochemical biosensors with enzymes have always been an attractive research topic in various sectors of biology due to their high specificity and reproducibility. In this study, we suggest a novel cellulosome scaffolding based multi-enzyme platform for the detection of isopropyl alcohol (IPA) using electrochemical biosensing assay. Three enzymes namely alcohol dehydrogenase (ADH), NADH oxidase (NOX), and peroxidase were isolated and successfully overexpressed in E.coli BL21 Rosetta respectively. The ability of enzymes to catalyze NAD+/NADH was confirmed using the absorption spectrum at 340㎚ wavelength. From the results, it was confirmed that the rate of oxidization of NADH by NOX and the rate of reduction of NAD+ by ADH were approximately 3 folds compared to control. In the synergistic reaction, comparative to the individual curves i.e. logistic for ADH and decaying for NOX, a straight line parallel to the baseline was obtained which confirmed that NAD+ was reduced to NADH and can be used by NOX for further oxidation simultaneously. Taken together, this study can be a progression in the area of electrochemical biosensors for the detection of IPA.

Direct Organogenesis in Geophila reniformis D. Don., an Important Medicinal Herb

Nisha, A.,Narasimhan, S.,Manjula, S.,Nair, G.M. The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.3

Adventitious multiple shoots were developed from leaf, petiole and internode explants of Geophila reniformis D. Don. on MS medium supplemented with various concentrations of $N^6$-benzylaminopurine (BAP) or Kinetin (KIN) alone or in combination with indole-3-acetic acid (IAA). Leaf showed maximum organogenetic potential, followed by petiole and internode. Murashige and Skoog (MS) medium supplemented with 22.22 $\mu{M}$ BAP and 4.57 $\mu{M}$ IAA induced maximum shoot buds from leaf explants. Internodal segments showed low potential of direct organogenesis. The regenerated shoots rooted the best in presence of 10.75 - 13.44 $\mu{M}$ $\alpha$-naphthalene acetic acid (NAA) along with 2.22 $\mu{M}$ BAP, and were successfully established in the field with a survival rate of 89.11%.

Capitalism: A Ghost Story by Arundhati Roy, Chicago: Haymarket Books, 2014.

Nisha Mary Mathew 부산대학교 한국민족문화연구소 2016 Localities Vol.6 No.-

e-Pharmacophore modeling and in silico study of CD147 receptor against SARS-CoV-2 drugs

Nisha Kumari Pandit,Simranjeet Singh Mann,Anee Mohanty,Sumer Singh Meena Korea Genome Organization 2023 Genomics & informatics Vol.21 No.2

Coronavirus has left severe health impacts on the human population, globally. Still a significant number of cases are reported daily as no specific medications are available for its effective treatment. The presence of the CD147 receptor (human basigin) on the host cell facilitates the severe acute respiratory disease coronavirus 2 (SARS-CoV-2) infection. Therefore, the drugs that efficiently alter the formation of CD147 and spike protein complex could be the right drug candidate to inhibit the replication of SARS-CoV-2. Hence, an e-Pharmacophore model was developed based on the receptor-ligand cavity of CD147 protein which was further mapped against pre-existing drugs of coronavirus disease treatment. A total of seven drugs were found to be suited as pharmacophores out of 11 drugs screened which was further docked with CD147 protein using CDOCKER of Biovia discovery studio. The active site sphere of the prepared protein was 101.44, 87.84, and 97.17 along with the radius being 15.33 and the root-mean-square deviation value obtained was 0.73 Å. The protein minimization energy was calculated to be -30,328.81547 kcal/mol. The docking results showed ritonavir as the best fit as it demonstrated a higher CDOCKER energy (-57.30) with correspond to CDOCKER interaction energy (-53.38). However, authors further suggest in vitro studies to understand the potential activity of the ritonavir.

Synergistic Nanozyme Complex to Fabricate Electrochemical Biosensor for Isopropyl Alcohol Detection

Nisha BHARDWAJ,Myeong Eun LEE,Sung Ok HAN 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10

BCL2 antagonist of cell death kinases, phosphatases, and ovarian cancer sensitivity to cisplatin

Nisha Bansal,Douglas C. Marchion,Elona Bicaku,Yin Xiong,Ning Chen,Xiaomang B. Stickles,Entidhar Al Sawah,Robert M. Wenham,Sachin M. Apte,Jesus Gonzalez-Bosquet,Patricia L. Judson,Ardeshir Hakam,Johnat 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.1

Objective: The BCL2 family proteins are critical mediators of cellular apoptosis and, as such, have been implicated as determinants of cancer cell chemo-sensitivity. Recently, it has been demonstrated that the phosphorylation status of the BCL2 antagonist of cell death (BAD) protein may influence ovarian cancer (OVCA) cell sensitivity to cisplatin. Here, we sought to evaluate how kinase and phosphatase components of the BAD apoptosis pathway influence OVCA chemo-sensitivity. Methods: Protein levels of cyclin-dependent kinase 1 (CDK1) and protein phosphatase 2C (PP2C) were measured by immunofluorescence in a series of 64 primary advanced-stage serous OVCA patient samples. In parallel, levels of cAMP-dependent protein kinase (PKA), AKT, and PP2C were quantified by Western blot analysis in paired mother/daughter platinum-sensitive/resistant OVCA cell lines (A2008/C13, A2780S/A2780CP, Chi/ChiR). BAD pathway kinase CDK1 was depleted using siRNA transfection, and the influence on BAD phosphorylation and cisplatin-induced apoptosis was evaluated. Results: OVCA patient samples that demonstrated complete responses to primary platinum-based therapy demonstrated 4-fold higher CDK1 (p<0.0001) and 2-fold lower PP2C (p=0.14) protein levels than samples that demonstrated incomplete responses. Protein levels of PP2C were lower in the platinum-resistant versus that shown in the platinum-sensitive OVCA cell line sub-clones. Levels of PKA were higher in all platinum-resistant than in platinum-sensitive OVCA cell line sub-clones. Selective siRNA depletion of CDK1 increased sensitivity to cisplatin-induced apoptosis (p<0.002). Conclusion: BAD pathway kinases and phosphatases, including CDK1 and PP2C, are associated with OVCA sensitivity to platinum and may represent therapeutic opportunities to enhance cytotoxic efficacy. Objective: The BCL2 family proteins are critical mediators of cellular apoptosis and, as such, have been implicated as determinants of cancer cell chemo-sensitivity. Recently, it has been demonstrated that the phosphorylation status of the BCL2 antagonist of cell death (BAD) protein may influence ovarian cancer (OVCA) cell sensitivity to cisplatin. Here, we sought to evaluate how kinase and phosphatase components of the BAD apoptosis pathway influence OVCA chemo-sensitivity. Methods: Protein levels of cyclin-dependent kinase 1 (CDK1) and protein phosphatase 2C (PP2C) were measured by immunofluorescence in a series of 64 primary advanced-stage serous OVCA patient samples. In parallel, levels of cAMP-dependent protein kinase (PKA), AKT, and PP2C were quantified by Western blot analysis in paired mother/daughter platinum-sensitive/resistant OVCA cell lines (A2008/C13, A2780S/A2780CP, Chi/ChiR). BAD pathway kinase CDK1 was depleted using siRNA transfection, and the influence on BAD phosphorylation and cisplatin-induced apoptosis was evaluated. Results: OVCA patient samples that demonstrated complete responses to primary platinum-based therapy demonstrated 4-fold higher CDK1 (p<0.0001) and 2-fold lower PP2C (p=0.14) protein levels than samples that demonstrated incomplete responses. Protein levels of PP2C were lower in the platinum-resistant versus that shown in the platinum-sensitive OVCA cell line sub-clones. Levels of PKA were higher in all platinum-resistant than in platinum-sensitive OVCA cell line sub-clones. Selective siRNA depletion of CDK1 increased sensitivity to cisplatin-induced apoptosis (p<0.002). Conclusion: BAD pathway kinases and phosphatases, including CDK1 and PP2C, are associated with OVCA sensitivity to platinum and may represent therapeutic opportunities to enhance cytotoxic efficacy.

Caffeic acid phenethyl ester inhibits pseudo-allergic reactions via inhibition of MRGPRX2/MrgprB2-dependent mast cell degranulation

Nisha Adhikari,Won-Sik Shim 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.9

Mast cells play essential role in allergic reactionsthrough the process called mast cell degranulation. Recent studies have found that a basic secretagogue compound48/80 (C48/80) induces non-IgE-mediated mastcell degranulation via activation of human Mas-related Gprotein-coupled receptor X2 (MRGPRX2) and mouse MrgprB2. Although previous studies have revealed that caff eicacid (CA) and its derivatives possess anti-allergic eff ects viaIgE-dependent manner, it is largely elusive whether thesecompounds have impact on MRGPRX2/MrgprB2 to exertinhibitory eff ects. Therefore, the present study investigatedwhether CA as well as its derivatives – rosmarinic acid (RA)and caff eic acid phenethyl ester (CAPE) – has the abilityto inhibit the activity of MRGPRX2/MrgprB2 to evokepseudo-allergic eff ects. As a result, it was found that CAPEinhibits C48/80-induced activation of MRGPRX2/MrgprB2,but neither CA nor RA showed discernible inhibition. Furthermore,the β-hexosaminidase release assay showed thatCAPE inhibits mouse peritoneal mast cell degranulationin both IgE-dependent and MrgprB2-dependent manners. Additionally, mouse paw edema induced by C48/80 was dramaticallysuppressed by co-treatment of CAPE, suggestingthat CAPE possesses a protective eff ect on C48/80-evokedpseudo-allergic reactions. The pretreatment of CAPE also signifi cantly decreased scratching bouts of mice evokedby C48/80, demonstrating that CAPE also has an anti-pruriticeff ect. Therefore, these data implicate that CAPE cansuppress pseudo-allergic reactions evoked by C48/80 viaMrgprB2-dependent manner. Finally, molecular dockinganalysis showed that CAPE is predicted to bind to humanMRGPRX2 in the region where C48/80 also binds, implyingthat CAPE can be a competitive inhibitor of MRGPRX2. Inconclusion, it is found that CAPE has the ability to inhibitMRGPRX2/MrgprB2, leading to the prevention of mast celldegranulation and further to the alleviation of mast cell reactions. These results indicate that CAPE as a CA derivativecould be developed as a new protective agent that exerts dualinhibition of mast cell degranulation mediated by IgE andMRGPRX2/MrgprB2.

In vitro antiproliferative study of curcumin loaded nano zeolitic imidazolate framework hybrid biomaterials on HeLa cells

Nisha G. Pillai,Archana K,이경엽,박수진,Asif A 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.79 No.-

Curcumin is a bioactive molecule having potential applications in medicalfield. Present study focuses tosynthesise the hybrid biomaterials of curcumin loaded nano zeolitic imidazolate frameworks of zinc andcobalt metals by solvothermal method. Influence of curcumin on surface morphology and thermalproperties of the hybrid biomaterials have been investigated. The framework hybrids are characterised byFTIR, XRD, SEM, TEM, BET surface area and TGA. The studies reveal that the hybrid biomaterials show highthermal stability with well identified morphology, decrease in particle size and large pore volume. Invitro antiproliferative studies corroborate that hybrid biomaterial displays greater cell growth inhibitoryperformance than that of pure metal organic framework and pure curcumin towards HeLa cells. In vitrocytotoxicity studies reveal that CCM–ZIF-67 hybrid biomaterial exhibits more specificity towardscancerous cells.