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In-vivo Glycosylation of genistein in E. coli using GmIF7GT from Glycine max
Niranjan Koirala,Ramesh Prasad Pandey,Na-Rae Lee,Jae Kyung Sohng 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Isoflavonoids, polyphenols are biologically active natural products which are abundantly present in soyabean seeds during development. Clinical studies have suggested positive effects of isoflavonoids in human health and nutrition, such as reduction in the risks of hormonally dependent cancers, menopausal symptoms, osteoporosis, and cardiovascular disease. However, the insolubility and instability of those polyphenols prevent the wide applications of these medicinally important compounds. Therefore, the generation of isoflavonoid derivatives by glycosylation gained much attention and interest. In this study, we used E.coli BL21 (DE3) as an expression host for gmIF7Gt, a Glycine max derived glycosyl transferase, cell biocatalyst for the production of glycosylated derivatives of genistein. The supplementation of 0.2mM of genistein in the growing induced culture of E. coli BL21 (DE3) harboring PET15b-GmIF7GT resulted novel spots in TLC. These spots were further analyzed by HPLC and LC-TOF ESI/MS. The exact molecular mass analysis confirmed the production of Genistein glucoside. Further, structural elucidation and product enhancement is necessary for large scale production of this compound. However, this study reveals a method that might be useful for the biosynthesis of glycosylated isoflavonoids and related compounds by in-vivo glycosylation.
ON CERTAIN SUBCLASSES OF STARLIKE AND CONVEX FUNCTIONS ASSOCIATED WITH WRIGHT FUNCTION
NIRANJAN BASAVANTHAPPA GATTI,Saurabh Porwal,NANJUNDAN MAGESH 장전수학회 2022 Advanced Studies in Contemporary Mathematics Vol.32 No.3
In the present investigation to obtain certain sufficient conditions for normalized Wright function to be in certain classes of starlike and convex functions defined in unit disc △ :={z∈C : |z|<}. Further, we obtain some inclusion relations and integral operator associated with Wright function.
Niranjan Koirala,Ramesh Prasad Pandey,Jae Kyung Sohng 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Methylation and glycosylation are two different but vital modification processes in plants to generate diversified secondary metabolites. Methylation increases the lipophilicity of compounds whereas glycosylation enhances the solubility. Most of the methylated and glycosylated natural compounds are significantly active against pathogens and cancers. In this study, an O-methyltransferase gene, designated as SpOMT-2884, was identified from Streptomyces peucetius ATCC 27952. To find the exact substrates for SpOMT-2884, we tested several compounds in-vitro. As a result SpOMT-2884 catalyzed O-methylation of flavonoids such a 7,8-dihydroxyflavone (7,8-DHF), quercetin, luteolin, fisetin and rutin. 7,8-DHF was found to be the best substrate. We further proceeded for in vivo biotransformation of 7,8-DHF where we used E. coli BL21 (DE3) expressing SpOMT-2884 cell, as a biocatalyst for the production of methylated derivative of 7,8-DHF. The supplementation of 0.2 mM of 7, 8-DHF in the growing induced culture of E.coli BL21 (DE3) harboring pET28-SpOMT-2884 recombinant resulted in the production of 7-methyl-8-hydroxyflavone which was confirmed by HPLC (Rt: 17 min), high resolution LC-QTOF-ESI/MS (m/z+ 269.08) and NMR spectroscopy. Further, this enzymatically synthesized methylated derivative of 7, 8-DHF was used as a substrate in vitro for glycosylation by Yjic, a glycosyltransferase from Bacillus licheniformis DSM13. This in-vitro reaction mixture analysis revealed the presence of glycosylated product which was confirmed by HPLC and LC-QTOF-ESI/MS (m/z+ 431.13). The glycosylation of the target was further supported by our findings from in silico docking analysis. Inparticular, this study demonstrated the potential for enzymatic biosynthesis of novel methylated cum glycosylated 7,8-DHF.
REtrospective Multicenter INdian Study of Derivo Embolization Device (REMIND): Periprocedural Safety
Niranjan Prakash Mahajan,Mudasir Mushtaq,Amit Bhatti,Sukalyan Purkayastha,Nitin Dange,Mathew Cherian,Vipul Gupta,Vikram Huded 대한신경중재치료의학회 2021 Neurointervention Vol.16 No.3
Purpose: The treatment of aneurysms with characteristics such as complex morphology, fusiform, blister-like, wide neck, or large size has been revolutionized with the introduction of flow diverters. Though flow diverters have several advantages over coiling, they also have certain important disadvantages such as the lack of immediate protection against rupture, the risk of ischemic stroke, the need for antiplatelet therapy, and long latency for complete effect. The Derivo Embolization Device (DED) is a second-generation self-expanding device that is claimed to be less thrombogenic than conventional devices. We retrospectively evaluated the periprocedural safety and risks associated with the DED across 5 centers in India. Materials and Methods: This is a multicentric, retrospective, observational study of DED, conducted at 5 high volume endovascular therapy centers in India from May 2018 to June 2020. Periprocedural demographic, clinical, and angiographic data were collected from a retrospective review of patient charts. Results: A total of 96 patients, including 56 (58.3%) females, aged between 16–80 years (60±12.7 years) harboring 106 aneurysms were studied. Seven (7.3%) were noted to harbor multiple aneurysms: 6 had 3 aneurysms each, while 1 patient had 5 aneurysms. The following aneurysm characteristics were noted: average size, 9.8±8.2 mm; average neck size, 6.9±8.5 mm; wide-necked (>4 mm), 63 (59.4%); giant (>25 mm), 8 (7.5%); and anterior circulation location, 98 (92.5%). Eighteen (17%) of these were ruptured. Additional balloon angioplasty was performed in 5 (5.2%) patients. Intraprocedural problems were encountered in 3 (3.1%), of which only 1 had clinical implications, the device fish-mouthing with stent thrombosis resulting in a malignant middle cerebral artery territory infarction. The modified Rankin scale at 3 months was worse in 1 patient. Conclusion: DED is a newer generation flow diverter stent with a low periprocedural complication rate.