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      • KCI등재

        Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function

        Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

        Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

      • KCI등재

        Development of label-free electrochemical lactose biosensor based on graphene/poly(1,5-diaminonaphthalene) film

        Binh Hai Nguyen,Binh Thanh Nguyen,Hanh Van Vu,Chuc Van Nguyen,Dzung Tuan Nguyen,Loc Thai Nguyen,Thu Thi Vu,Lam Dai Tran 한국물리학회 2016 Current Applied Physics Vol.16 No.2

        In this work, a lactose biosensor was developed by co-immobilizing b-galactosidase (b-Gal) and glucose oxidase (GOx) on microelectrodes pre-modified with Pt/graphene/P(1,5-DAN) for estimation of lactose in dairy products to prevent lactose intolerance. The Pt microelectrode was modified with graphene and 1,5-polydiaminonaphthalene film. Graphene was synthesized by chemical vapor deposition on copper tape and manually transferred to the electrode surface. Polymeric P(1,5-DAN) was grafted on top of the graphene film by electropolymerization. Modified surface of the electrode was characterized by Raman spectra analysis, FE-SEM, AFM and cyclic voltammetry. The results indicated that deposition graphene film on electrode surface induced considerable enhancement in current signal, over 20 times as high as the uncoated electrode surface. The developed sensor was successfully used to determine lactose in model samples with sensitivity, correlation coefficient (R2) and limit of detection (LOD) estimated to be 1.33 mA/(mgml1), 0.995 and 1.3 mg/ml, respectively. The combined graphene and conductive P(1,5-DAN) could serve as a novel sensing platform on electrochemical sensors with superior sensitivity.

      • KCI등재

        Two New C-Glucosyl Benzoic Acids and Flavonoids from Mallotus nanus and Their Antioxidant Activity

        Phan Van Kiem,Nguyen Thi Mai,Chau Van Minh,Nguyen Huu Khoi,Nguyen Hai Dang,Nguyen Phuong Thao,Nguyen Xuan Cuong,Nguyen Hoai Nam,Nguyen Xuan Nhiem,Yvan Vander Heyden,Joëlle Quetin-Leclercq,김교남,장해동,김영호 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

        Two new 2-C-β-D-glucopyranosyl benzoic acid derivatives named mallonanosides A (1) and B (2) were isolated from the methanolic extract of the leaves of Mallotus nanus along with five known flavonoids, kaempferin (3), juglanin (4), quercitrin (5), myricitrin (6), and rhoifolin (7). Their structures were established on the basis of spectral and chemical evidence. Their antioxidant activities were shown to depend on the number of hydroxyl groups, and the location and species of sugar moiety.

      • KCI등재

        Applications of Magnetite Nanoparticles for Water Treatment and for DNA and Cell Separation

        Nguyen Hoang Hai,Nguyen Chau,Nguyen Hoang Luong,Nguyen Thi Van Anh,Phan Tuan Nghia 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3

        Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope. Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope.

      • A Novel Robotic Platform for Aerial Manipulation Using Quadrotors as Rotating Thrust Generators

        Nguyen, Hai-Nguyen,Park, Sangyul,Park, Junyoung,Lee, Dongjun IEEE 2018 IEEE transactions on robotics Vol.34 No.2

        <P>We propose a novel robotic platform for aerial operation and manipulation, a spherically connected multiquadrotor (SmQ) platform, which consists of a rigid frame and multiple quadrotors that are connected to the frame via passive spherical joints and act as distributed rotating thrust generators to collectively propel the frame by adjusting their attitude and thrust force. Depending on the number of quadrotors and their configuration, this SmQ platform can fully (or partially) overcome the issues of underactuation of the standard multirotor drones for aerial operation/manipulation (e.g., body-tilting with sideway gust/force, dynamic interaction hard to attain, complicated arm–drone integration, etc.). We present the dynamics modeling of this SmQ platform system and establish the condition for its full actuation in SE(3). We also show how to address limited range of spherical joints and rotor saturations as a constrained optimization problem by noticing the similarity with the multifingered grasping problem under the friction-cone constraint. We then design and analyze feedback control laws for the S3Q and S2Q systems as a combination of high-level Lyapunov control design and low-level constrained optimization and show that the (fully actuated) S3Q system can assume any trajectory in SE(3), whereas the S2Q system in <TEX>$\Re^3\, \times$</TEX> S<TEX>$^2$</TEX> with its unactuated dynamics is still internally stable. Experiments are also performed to show the efficacy of the theory.</P>

      • KCI등재후보

        Improving Utilization of GPS Data for Urban Traffic Applications

        Nguyen Duc Hai,Nguyen Tan Phuc,Doan Khue,Ta Ho Thai Hai,Pham Tran Vu,Huynh Nam,Le Thanh Van 한국인터넷방송통신학회 2015 International Journal of Internet, Broadcasting an Vol.7 No.1

        The use of Intelligent Transportation System (ITS) is promising to bring better solutions for managing and handling the city traffic. This system combines many fields in advanced technology such as Global Positioning System (GPS), Geographic Information System (GIS) and so on. The basement of applications in ITS is the effective collections and data integration tools. The purpose of our research is to propose solutions which involve the use of GPS time series data collected from GPS devices in order to improve the quality of output traffic data. In this study, GPS data is collected from devices attached to vehicles travelling on routes in Ho Chi Minh City (HCMC). Then, GPS data is stored in database system to serve in many transportation applications. The proposed method combines the data usage level and data coverage to improve the quality of traffic data.

      • KCI등재

        Large Magnetocaloric Effect above 300 K and Magnetoresistance in (La0.5Pr0.5)1-xPbxMnO3 Perovskites

        Nguyen Chau,Duong Thi Hanh,Bui Cong Tinh,Nguyen Hoang Luong,Nguyen Duc Tho,Nguyen Hoang Hai 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

        Perovskite compounds (La0.5Pr0.5)₁- xPbxMnO₃ (x = 0.1 - 0.5) with orthorhombic structure were prepared by using a solid-state reaction technique. In the studied samples, the spin-glass-like state occurs at low temperatures and the Curie temperature, Tc, increases from 170 K (x = 0.1) to 336 K (x = 0.5) because of a strong double exchange. The magnetic entropy change, │△Sm│, reached its largest value of 2.06 J/kg·K at △H = 13.5 kOe for the sample with x = 0.4 (Tc = 323 K). Due to the large │△Sm│ and high Tc, these materials are suggested for use as active magnetic refrigerants for magnetic refrigeration technology at temperatures above room temperature. While the conductivity of the samples with x = 0.1 and 0.2 exhibits only a semiconducting behavior over the whole measured temperature range, there is insulator-metallic phase transition on the R(T) curves of the samples with x = 0.3 - 0.5. The magnetoresistance of the samples was measured and large values for MR were found. Perovskite compounds (La0.5Pr0.5)₁- xPbxMnO₃ (x = 0.1 - 0.5) with orthorhombic structure were prepared by using a solid-state reaction technique. In the studied samples, the spin-glass-like state occurs at low temperatures and the Curie temperature, Tc, increases from 170 K (x = 0.1) to 336 K (x = 0.5) because of a strong double exchange. The magnetic entropy change, │△Sm│, reached its largest value of 2.06 J/kg·K at △H = 13.5 kOe for the sample with x = 0.4 (Tc = 323 K). Due to the large │△Sm│ and high Tc, these materials are suggested for use as active magnetic refrigerants for magnetic refrigeration technology at temperatures above room temperature. While the conductivity of the samples with x = 0.1 and 0.2 exhibits only a semiconducting behavior over the whole measured temperature range, there is insulator-metallic phase transition on the R(T) curves of the samples with x = 0.3 - 0.5. The magnetoresistance of the samples was measured and large values for MR were found.

      • Knowledge and Attitude Towards Tobacco Smoking among 13-15 Year-Old School Children in Viet Nam - Findings from GYTS 2014

        Nguyen, Thanh Huong,Nguyen, Trung Kien,Kim, Bao Giang,Hoang, Van Minh,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

        Studies have shown that smoking is a learnt behavior, often initiated during adolescence. This paper aims to describe tobacco-related knowledge, attitude and associations among school adolescents aged 13-15 with exposure to anti-smoking information. Using data from the Global Youth Tobacco Survey (GYTS) in Viet Nam, 2014, knowledge was measured through 4 questions about tobacco use, and attitude was assessed through 3 questions on personal, social and environmental aspects. Students giving most anti-tobacco responses to all questions were considered as having correct knowledge or appropriate attitude or both. Access to anti-smoking information was determined by exposure to any media messages on tobacco control during the past 30 days and teaching in school about the danger of tobacco use during the past 12 months. A substantial percentage of students thought that being near others who smoke might be harmful to them and smoking is harmful to health (89.4% and 89.6% respectively). However, only 46.4% reported that it is definitely difficult to quit smoking and 66.9% thought that smoking for only 1 or 2 years, once stopped, is harmful to health. Slightly more than half of the respondents reported appropriate attitude that young smokers have fewer friends than others and smoking makes them less attractive and less comfortable at social events. Noticing anti-smoking messages in the media together with having lessons in school about the dangers of tobacco substantially increased the likelihood of having correct knowledge, appropriate attitude and both. Despite relatively high awareness about smoking harms, effective educational communication is still highly needed to improve the level of comprehensive knowledge and an appropriate attitude regarding tobacco use.

      • SCOPUS

        Factors Affecting Online Reservation Decisions through Hotel Websites: An Empirical Study from Can Tho City, Vietnam

        Hai Quynh Tram NGUYEN,Yen Nhi LE,Ly Giau LAM,Thi Yen Nhi LE,Trieu Di NGUYEN,Thi Kim Yen PHAM,Trong Luan NGUYEN 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.5

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Many consumers are opting for online booking over traditional booking systems. Customers can actively seek out information about hotels and lodging services, as well as book rooms, at any time and from any location. Customers also feel more supported when they interact with virtual assistants or professionals. Recognizing this issue, several hotels have focused on improving their websites by incorporating aspects that encourage customers to book directly through the hotel’s website. The study’s goal is to discover what factors impact people’s decisions to book a hotel stay through the hotel’s website. Therefore, hotel managers and owners can make decisions to improve the hotel website to attract residents to Can Tho City. The factors are website quality, affective commitment, social presence, and e-trust that affect customers’ decision to book through the hotel website. The study uses quantitative methods to collect data from 180 residents living in Can Tho. Through data analysis on SPSS and Amos software, the research results show that three factors considered, namely website quality, affective commitment, and social presence, positively influence customers’ booking decisions. This finding also suggests that e-trust is less critical to residents in Can Tho City, different from what the study had predicted.

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