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Neupane, Ganesh Prasad,Kim, Dong-Min,Kim, Sung Hun,Lee, Bok Kwon American Society for Microbiology 2010 Antimicrobial Agents and Chemotherapy Vol.54 No.9
<B>ABSTRACT</B><P>Paratyphoid fever is considered an emerging systemic intracellular infection caused by <I>Salmonella enterica</I> serotypes Paratyphi A, B, and C. We performed <I>in vitro</I> time-kill studies on three clinical isolates of nalidixic acid-resistant <I>Salmonella</I> serotype Paratyphi (NARSP) with different concentrations of ciprofloxacin and cefotaxime to identify combinations of antibiotics with synergistic activity against paratyphoid fever. Furthermore, we identify the frequency of mutations to ciprofloxacin, cefotaxime, and rifampin resistance and also sequenced the <I>gyrA</I>, <I>gyrB</I>, <I>parC</I>, and <I>parE</I> genes to identify the cause of resistance in NARSP. When the activity of ciprofloxacin at 0.75× MIC (0.012 to 0.38 μg/ml) with cefotaxime at the MIC (0.125 to 0.25 μg/ml) against all three NARSP isolates was investigated, synergy was observed at 24 h, and the bacterial counts were reduced by >3 log10 CFU/ml. This synergy was elongated for up to 72 h in two out of three isolates. When ciprofloxacin at 0.75× MIC (0.012 to 0.38 μg/ml) was combined with cefotaxime at 2× MIC (0.25 to 0.50 μg/ml), synergy was prolonged for up to 72 h in all three isolates. Both <I>Salmonella</I> serotype Paratyphi A isolates carried single mutations in codon 83 of the <I>gyrA</I> gene and codon 84 of the <I>par</I>C gene that were responsible for their reduced susceptibility to ciprofloxacin, while no mutations were found in the <I>gyrB</I> or <I>parE</I> gene. The ciprofloxacin-plus-cefotaxime regimen was very effective in reducing the bacterial counts at 24 h for all three isolates, and this combination therapy may be helpful in reducing the chance of the emergence of fluoroquinolone-resistant mutants in patients with severe paratyphoid fever.</P>
Neupane, Guru P.,Tran, Minh Dao,Kim, Hyun,Kim, Jeongyong Hindawi Limited 2017 Journal of nanomaterials Vol.2017 No.-
<P>Monolayer MoS2 (1L-MoS2) is an ideal platform to examine and manipulate two dimensionally confined exciton complexes, which provides a large variety of modulating the optical and electrical properties of 1L-MoS2. Extensive studies of external doping and hybridization exhibit the possibilities of engineering the optical and electrical performance of 1L-MoS2. However, biomodifications of 1L-MoS2 and the characterization and applications of such hybrid structures are rarely reported. In this paper, we present a bio-MoS2 hybrid structure fabricated by laterally stretching strands of DNAs on CVD-grown 1L-MoS2. We observed a strong modification of photoluminescence and Raman spectra with reduced PL intensity and red-shift of PL peak and Raman peaks, which were attributed to electron doping by the DNAs and the presence of tensile strain in 1L-MoS2. Moreover, we observed a significant enhancement of electric mobility in the DNA/1L-MoS2 hybrid compared to that in the pristine 1L-MoS2, which may have been caused by the induced strain in 1L-MoS2.</P>
Enhanced neuroinflammatory responses after systemic LPS injection in IL-32β transgenic mice
Neupane, Sabita,Srivastav, Sunil,Bhurtel, Sunil,Katila, Nikita,Shadfar, Sina,Park, Pil-Hoon,Hong, Jin Tae,Choi, Dong-Young Elsevier 2018 Journal of chemical neuroanatomy Vol.94 No.-
<P><B>Abstract</B></P> <P>IL-32 is a proinflammatory cytokine, and involved in various diseases including infection, inflammation, and cancer. However, effects of IL-32 on neuroinflammation remain obscure. Herein, we examined the effects of IL-32β on systemic LPS-induced neuroinflammation using IL-32β transgenic (Tg) mice. IL-32β wild type (WT) and Tg mice received LPS injection (5 mg/kg, i.p.), and then neuroinflammatory responses were evaluated. Systemic LPS caused remarkable gliosis in the brain at 12 h regardless of genotypes. The gliosis in WT mice was sustained by 24 h, whereas it became more severe in Tg mice by 24 h. Proinflammatory cytokines and proteins were increased at 12 h both in WT and Tg brains. The elevated levels of TNFα and VCAM-1were not altered over time, while levels of IL-6, IL-1β and iNOS were dropped in WT mice. In contrast, elevated levels IL-6, IL-1β, iNOS and VCAM-1 were sustained, and level of TNFα was augmented in Tg brains by 24 h. Interestingly, level of IL-10 mRNA in Tg mice was remarkably higher than in WT mice at 0 h, which was decreased at 12 h and maintained by 24 h. In WT brain, mRNA level of IL-10 was raised at 12 h after LPS injection, and further increased at 24 h. Activation of NF-κB signaling pathway was detected in glia cells after LPS injection which was exaggerated at 24 h in Tg mice in comparison to WT mice. These results indicate that IL-32β enhances neuroinflammatory responses caused by systemic LPS, and this might be attributable to prolonged activation of NF-κB signaling pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of IL-32β on neuroinflammation were evaluated. </LI> <LI> Enhanced neuroinflammation was observed in IL-32β transgenic mice. </LI> <LI> Prolonged activation of NF-κB might be related to extended neuroinflammation. </LI> </UL> </P>
Neupane,Deepak,Park,Jae-Woo 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-
A novel group of surfactants, called gemini surfactants, are being suggested for decontamination of water, soils and sediments polluted with nonionic organic compounds(NOCs). A combination of anionic gemini surfactant and positively-charged aluminum oxide particle are studied for their effectiveness in removing NOCs from aqueous phase. Experimental comparisons are made with conventioanl surfactant-treated and dianionic surfactant-treated aluminum oxide particles. Results indicate that gemini surfactant-treated aluminum oxide particles act as a more efficient sorptive phase for NOCs, which suggests the use of gemini surfactants rather than conventioanl surfactants for practical application of adsorbed surfactant phase to decontaminate NOC-polluted environment.
Neupane, Lok Nath,Han, Song Yee,Lee, Keun-Hyeung The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.44
<P>An amphiphilic dipeptide (<B>1</B>) bearing pyrene and phenylboronic acid was demonstrated as a unique example of a ratiometric sensing system for sugars by reversibly converting the peptide aggregates into the monomer form of the complex with sugars in aqueous solutions.</P> <P>Graphic Abstract</P><P>An amphiphilic dipeptide (<B>1</B>) bearing pyrene and phenylboronic acid was demonstrated as a unique example of a ratiometric sensing system for sugars by reversibly converting the peptide aggregates into the monomer form of the complex with sugars in aqueous solutions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc01439a'> </P>