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Naru Kim,Huisong Lee,Seog Ki Min,Hyeon Kook Lee 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.94 No.5
Purpose: To compare survival outcomes between bile duct segmental resection (BDR) and pancreatoduodenectomy (PD) for the treatment of middle and distal bile duct cancer. Methods: From 1997 to 2013, a total of 96 patients who underwent curative intent surgery for middle and distal bile duct cancer were identified. The patients were divided into 2 groups based on the type of operation; 20 patients were included in the BDR group and 76 patients were in the PD group. We retrospectively reviewed the clinical outcomes. Results: The number of lymph nodes (LNs) was significantly greater in patients within the PD group compared to the BDR group. The total number of LNs was 6.5 ± 8.2 vs. 11.2 ± 8.2 (P = 0.017) and the number of metastatic LNs was 0.4 ± 0.9 vs. 1.0 ± 1.5 (P = 0.021), respectively. After a median follow-up period of 24 months (range, 4–169 months), the recurrence-free survival of the PD group was superior to that of the BDR group (P = 0.035). In the patients with LN metastases, the patients undergoing PD had significantly better survival than the BDR group (P < 0.001). Conclusion: Surgeons should be cautious in deciding to perform BDR for middle and distal common bile duct cancer. PD is recommended if LN metastases are suspected.
Development of High Cordycepin-Producing Cordyceps militaris Strains
( Naru Kang ),( Hyun-hee Lee ),( Inmyoung Park ),( Young-su Seo ) 한국균학회 2017 Mycobiology Vol.45 No.1
Cordyceps militaris, known as Dong-Chong-Xia-Cao, produces the most cordycepin among Cordyceps species and can be cultured artificially. For these reasons, C. militaris is widely used as herb or functional food in the East Asia. In this study, we developed a new strain of C. militaris that produces higher cordycepin content than parent strains through mating-based sexual reproduction. Twenty parent strains were collected and identified as C. militaris based on internal trasncrived spacer and rDNA sequences. Seven single spores of MAT 1-1 idiomorph and five single spores of MAT 1-2 idiomorph were isolated from 12 parent strains. When 35 combinations were mated on the brown rice medium with the isolated single spores, eight combinations formed a stroma with a normal perithecia and confirmed mated strains. High pressure liquid chromatography analysis showed that mated strain KSP8 produced the most cordycepin in all the media among all the tested strains. This result showed due to genetic recombination occurring during the sexual reproduction of C. militaris. The development of C. militaris strain with increased cordycepin content by this approach can help not only to generate new C. militaris strains, but also to contribute to the health food or medicine industry.
Clinical Significance of Intraoperative Bile Culture in Surgery Including Bile Duct Resection
( Youngju Ryu ),( Naru Kim ),( Yung Hun You ),( In Woong Han ),( Jin Seok Heo ),( Dong Wook Choi ),( Sang Hyun Shin ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: It is widely accepted that intraoperative bacterial infection may potentially result in a worse postoperative outcomes. The purpose of this study is to analyze the microbiology of intraoperative bile smear culture test and the correlation between the results of culture and postoperative outcomes in bile duct resection operation. Methods: The data was prospectively collected from 235 patients who underwent bile duct resection at Samsung Medical Center for one year from October 2018 to September 2019. The diseases included in the data are periampullary cancer, gallbladder cancer, hilar cholangiocarcionoma, and intrahepatic cholangiocarcinoma. Intraoperative bile smear test was performed in operation, and the included operation was pancreaticoduodenectomy and liver resection surgery with bile duct resection. Specimens were obtained from culture swab of bile drained during bile duct resection. Results: Of the 235 patients, microorganism was isolated in 141 patients (60%). The predominant microorganisms grown from the intraoperative bile cultures were Enterococcus faecalis (38 cultures, 27.0%), Enterococcus faecium (32 cultures, 22.7%), Klebsiella pneumoniae and Enterobacter cloacae (28 cultures, 19.9%). In postoperative complication, the positive results of intraoperative bile cultures was related with Clavien-Dindo Classification≥Ⅲ (OR3.117, 95%CI:1.498-6.485, P=0.002). Also, it was a risk factors for occurrence of surgical site infection (OR3.266, 95%CI:1.237-8.621, P=0.013) and intra-abdominal abscess (OR1.145, 95%CI:1.057-1.240, P=0.003). In addition, the incidence of postoperative pancreatic fistula was increased in patients with microorganisms grown in bile (OR1.974, 95%CI:1.098-3.549, P=0.022). Conclusions: Smear positivity of intraoperative bile fluid is associated with occurrence of major complication. It was risk factor for surgical site infection and intra-abdominal abscess.
( Hyun-hee Lee ),( Naru Kang ),( Inmyoung Park ),( Jungwook Park ),( Inyoung Kim ),( Jieun Kim ),( Namgyu Kim ),( Jae-yun Lee ),( Young-su Seo ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7
Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dongchung- ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3`- deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating-type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of C. militaris were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of C. militaris. After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with MAT1-1 and the others with MAT1-2. Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4- bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new C. militaris strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing C. militaris strains.
H.N. Yoon,Joonho Seo,Naru Kim,H.M. Son,H.K. Lee Techno-Press 2023 Advances in concrete construction Vol.15 No.1
Autogenous healing of concrete can be helpful in structural maintenance by healing cracks using a healing material created by the precipitation of calcite and by the hydration of unhydrated binder around the cracks. Against this backdrop, this study investigated the physicochemical properties and autogenous healing performance of ternary blended binder composed of ordinary Portland cement (OPC), blast furnace slag (BFS), and calcium sulfoaluminate (CSA) clinker. Ternary blended binders with various contents of OPC-BFS-CSA clinker were prepared, and their physicochemical properties and autogenous healing performances were examined using various analytical techniques and visually observed using a microscope. The obtained results indicated that increase in the BFS content accompanied the increased the amount of unreacted BFS even after 28 days of curing and had a positive effect on the autogenous healing performance due to its latent hydration. However, replacing the CSA clinker did not increase the autogenous healing performance owing to an insufficient sulfate source for the formation of ettringite. The main precipitates around the cracks were calcite, C-S-H. Other hydration products such as portlandite, monosulfate, and ettringite, which were not found in the Raman and scanning electron microscope analyses.