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Raman, Jegadeesh,Sivakumar, Archana,Lakshmanan, Hariprasath,Raaman, Nanjian,Shin, Hyun-Jae The Korean Society of Mushroom Science 2021 한국버섯학회지 Vol.19 No.3
버섯 다당류는 면역 조절 기능, 항암 및 항산화 활성을 비롯하여 항바이러스 활성과 방사선스트레스의 경감등 인체에 유익한 다양한 생리활성을 나타낸다. 본 연구에서는 분홍느타리버섯 (Pleurotus djamor var. roseus Corner)으로부터 뜨거운 물과 에탄올 침전을 이용하여 5.6%의 수율로 갈색을 띠는 경화된 다당류(CPs)를 순차적으로 추출하였다. 이렇게 추출된 CP는 Diethylaminoethyl cellulose(DEAE) 및 sepharose-6B 컬럼 분리를 통해 4개의 분획을 얻었고 각 분획의 총 글루칸 함량은 각각 76.85%, 2.95%, 75.08%, 1.46%로 밝혀졌다. 이중 가장 높은 수율의 분획(PP)으로부터 300 mg의 백색 분말이 얻어 졌으며, 박층 크로마토그래피(TLC)와 푸리에 변환 적외선 분광법(FTIR)의 결과로부터 자일로펜토스 유형의 화합물과 함께 다당류 부분의 존재를 확인하였다. PP의 항산화 활성은 1,1-diphenyl-2-picryl-hydrazyl(DPPH) 자유라디칼 소거 분석 및 슈퍼옥사이드 라디칼 소거 분석을 통하여 높은 활성을 나타냄을 확인하였다. PP분획에는 페놀, 단백질 및 단순 탄수화물이 없는 정제된 베타글루칸이 주 구성성분으로, 정제된 다당류가 천연 항산화제로 사용될 수 있음을 알 수 있었다. Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.
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Palaniappan Selvakumar,Seetharaman Rajasekar,Anam Giridhar Babu,Karupannan Periasamy,Nanjian Raaman,Modem Sudhakara Reddy 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5
Two hybrid strains were obtained through protoplasmic fusion between Pleurotus ostreatus var. florida and Pleurotus djamor var. roseus. The hybrid strains showed intermediate morphology (colony morphology, cultural characteristics, and fruiting body) to parents. The size and RFLP pattern of two recombinant hybrids were similar, and differed from the parental Pleurotus species. The internal transcribed spacer sequence data revealed that hybrids showed close clad with variety of Pleurotus spp. The hybrid strains showed maximum biological efficiency (138.97%) than the parental strains (110.48-119.19%). In addition, maximum proximate composition, energy values (268.28 kcal), vitamins (thiamine, riboflavin, pyridoxine, folic acid, and calcium pentothenate) and mineral contents (potassium, phosphorus, iron, calcium, and zinc) were recorded in hybrid strain. The hybrid Pleurotus sp. HS would be an important resource of food for mankind, utilize variety of agricultural waste, high amount of nutritional properties and essential food for human health.
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Synthesis, anti-microbial activity and molecular docking studies on triazolylcoumarin derivatives
SATHEESHKUMAR, CHINNADURAI,RAVIVARMA, MAHALINGAM,ARJUN, PANDIAN,SILAMBARASAN, VAITHIYANATHAN,RAAMAN, NANJIAN,VELMURUGAN, DEVADASAN,SONG, CHANGSIK,RAJAKUMAR, PERUMAL Springer-Verlag 2015 Journal of chemical sciences Vol.127 No.3
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