Organizational Change and Inertia: A Comparative Case Study of Two Korean Telecommunication Companies

Namshin Cho,Seog K.Kwun 한국인사조직학회 1997 한국인사ㆍ조직학회 발표논문집 Vol.14 No.-

Characterization of Aldolase from Methanococcus jannaschii by Gas Chromatography

NamShin, Jeong-E.,Kim, Mi-Jung,Choi, Ji-Ah,Chun, Keun-Ho Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

The products of reactions catalyzed by Methanococcus. jannaschii (Mj) aldolase using various substrates were identified by gas chromatography (GC). Although Mj aldolase is considered a fuculose-1-phosphate aldolase based on homology searching after gene sequencing, it has not been proven to be a fuculose-1-phosphate aldolase based on its reaction products. Mj aldolase was found to catalyze reactions between glycoaldehyde or D, L-glyceraldehyde and DHAP (dihydroxyacetone phosphate). Before performing GC the ketoses produced were converted into peracetylated alditol derivatives by sequential reactions, i.e., dephosphorylation, $NaBH_4$ reduction, and acetylation. By comparing the GC data of final products with those of standard alditol samples, it was found that the enzymatic reactions with glycoaldehyde, D-glyceraldehyde, and D, L-glyceraldehyde produced D-ribulose-1-phosphate, D-psicose-1-phosphate, and a mixture of D-psicose and L-tagatose-1-phosphate, respectively. These results provide direct evidence that Mj aldolase is a fuculose-1-phosphate aldolase.

PC 기반의 Eureka-147 방식 실시간 DAB 수신기

조남신(Namshin Cho),조재희(Jaehee Cho),방극준(Keukjoon Bang),김광철(Kwangchul Kim),송환석(Hwanseok Song),박현철(Hyuncheol Park),홍대식(Daesik Hong) 한국통신학회 1999 한국통신학회 학술대회논문집 Vol.1999 No.11

ASePCR: alternative splicing electronic RT–PCR in multiple tissues and organs

Kim, Namshin,Lim, Dajeong,Lee, Sanghyuk,Kim, Heebal Oxford University Press 2005 Nucleic acids research Vol.33 No.2

<P>RT–PCR is one of the most powerful and direct methods to detect transcript variants due to alternative splicing (AS) that increase transcript diversity significantly in vertebrates. ASePCR is an efficient web-based application that emulates RT–PCR in various tissues. It estimates the amplicon size for a given primer pair based on the transcript models identified by the reverse e-PCR program of the NCBI. The tissue specificity of each PCR band is deduced from the tissue information of expressed sequence tag (EST) sequences compatible with each transcript structure. The output page shows PCR bands like a gel electrophoresis in various tissues. Each band in the output picture represents a putative isoform that could happen in a tissue-specific manner. It also shows the EST alignment and tissue information in the genome browser. Furthermore, the user can compare the AS patterns of orthologous genes in other species. The ASePCR, available at , supports the transcriptome models of the RefSeq, Ensembl, ECgene and AceView for human, mouse, rat and chicken genomes. It will be a valuable web resource to explore the transcriptome diversity associated with different tissues and organs in multiple species.</P>

ASmodeler: gene modeling of alternative splicing from genomic alignment of mRNA, EST and protein sequences

Kim, Namshin,Shin, Seokmin,Lee, Sanghyuk Oxford University Press 2004 Nucleic acids research Vol.32 No.suppl2

<P>Alternative splicing is in important mechanism of modulating gene function and expression which greatly expands transcriptome diversity. ASmodeler is a novel web-based utility that finds gene models including alternative splicing events from genomic alignment of mRNA, EST and protein sequences. User-supplied sequences are aligned against the genome map using the BLAT and SIM4 programs. Resulting exon connectivity is analyzed by applying graph-theoretic methods to build all possible gene models including splice variants. The algorithm essentially combines the genome-based sequence clustering and transcript assembly procedures in a coherent fashion. In addition to the user-supplied sequences, UniGene clusters and many well-known gene predictions such as Genscan, Ensembl and Acembly may be included in gene modeling. The current implementation supports human, mouse and rat genomes. ASmodeler is available at http://genome.ewha.ac.kr/ECgene/ASmodeler/.</P>

ChimerDB - Database of Chimeric Sequences in the GenBank

Kim, Namshin,Shin, Seokmin,Cho, Kwang-Hwi,Lee, Sanghyuk Korea Genome Organization 2004 Genomics & informatics Vol.2 No.2

Fusion proteins resulting from chimeric sequences are excellent targets for therapeutic drug development. We developed a database of chimeric sequences by examining the genomic alignment of mRNA and EST sequences in the GenBank. We identified 688 chimeric mRNA and 20,998 chimeric EST sequences. Including EST sequences greatly expands the scope of chimeric sequences even though it inevitably accompanies many artifacts. Chimeric sequences are clustered according to the ECgene ID so that the user can easily find chimeric sequences related to a specific gene. Alignments of chimeric sequences are displayed as custom tracks in the UCSC genome browser. ChimerDB, available at http://genome.ewha.ac.kr/ECgene/ChimerDB/, should be a valuable resource for finding drug targets to treat cancers.

왜 협상이 결렬되는가 : 행태적 의사결정관점 및 이해관계자 파워 관점에서 본 사례연구

조남신(Namshin Cho) 한국인사ㆍ조직학회 2007 인사조직연구 Vol.15 No.1

본 논문은 K대학교의 노사간 임금협상이 양측의 상당한 노력에도 불구하고 결렬되는 과정을 조명하였다. 이를 위해 행태적 의사결정관점과 이해관계자 파워관점을 채택하였다. 기존 협상연구의 주류를 이루는 행태적 의사결정관점을 통해서는 혼합형태의 동기, 자신의 대안에 대한 과신, 정보의 잘못된 해석, 협상이슈에의 감정적 몰입 등을 주요한 장애요인으로 설명하였다. 본 연구에서 새로이 제시한 이해관계자 파워관점에서는 협상참여자 및 이해관계자의 복잡성과 그 영향력, 협상참여자의 파워강화 의도, 파워강화를 위한 협상의제 제기, 협상대표에 대한 이해관계자들 간의 입장상충 등이 협상 결렬과 연관되어 있는 것으로 설명하였다. 이들 각각의 요인들에 대해 명제를 제시하고 시사점 및 추후 연구과제를 논의하였다. This study illuminates the process through which a series of negotiations resulted in failures, i.e., impasse with all the efforts of each party. Existing studies take either a structural approach based on rational perspective or a behavioral decision-making approach based on socialpsychological perspective. They do not fully explain the process through which negotiations result in impasse, particularly those in labor-management relations such as shown in this paper. This paper adopts Allison’s (1971) perspective to suggest an alternative view to explain impasse, a stakeholder power view. It takes a behavioral decision-making view and a stakeholder power view to explain a labor-management negotiation case of a university that resulted in an impasse. The author participated in and observed a series of labor-management negotiations as a member of the negotiation team on the management side at a university in Korea. This study develops a case based on the participative observation, documents, announcements, and conversations. The case is interpreted from the two perspectives and propositions are suggested respectively. From a behavioral decision-making perspective which assumes bounded rationality of negotiators as decision makers, the following propositions are proposed to illuminate the critical barriers to successful negotiation results. (1) A negotiation with mixed-motives is likely to result in an impasse as much as a distributive negotiation because negotiators often do not recognize the possibilities of integrative outcomes. (2) When negotiators have over-confidence in their own alternatives compared with the actual ones, a negotiation is likely to result in an impasse. (3) Negotiators who misinterpret the interests of the opponents with limited information and communication are less likely to reach an agreement. (4) Negotiators who focus, with affective involvement, on their positions rather than interests are less likely to reach an agreement. A stakeholder power perspective assumes that a number of stakeholders participate in negotiations with their own interests to increase personal and group power. The negotiation outcomes are resultants of ‘power games’ among the participants who often make non-rational decisions. The following propositions are suggested. (5) A negotiation that has a larger number of participants and stakeholders who exert stronger power is like to result in an impasse. (6) Negotiators who have stronger intentions to increase their own power within the subgroup they belong to are less likely to reach an agreement. (7) Negotiators who select issues with an intention to increase their own personal or subgroup power are less likely to reach an agreement. (8) A negotiation whose stakeholders have more conflict of interests is likely to result in an impasse. Implications to avoid or overcome barriers to agreement under bounded rationality are suggested as follows. First, negotiators need to intentionally promote communication between the parties regarding the procedures and issues of negotiation in order to explore the possibility of integrative outcomes. Second, negotiators need to develop open environment with the negotiation team so that they can freely evaluate and discuss about their BATNA. Third, negotiators need to build trust between the parties through informal as well as formal communications. Fourth, negotiators need to make efforts to make and keep rapport with the other party in order not to become affectively involved in their positions. From a stakeholder power view, the following implications are suggested even though they may be difficult to implement. Fifth, negotiators need to make efforts to manage stakeholders so that they can keep the balance of power among the stakeholders. Sixth, negotiators need to adopt rule oriented approach in order to complement power oriented approach. Seventh,

ECgene: genome-based EST clustering and gene modeling for alternative splicing.

Kim, Namshin,Shin, Seokmin,Lee, Sanghyuk Cold Spring Harbor Laboratory Press 2005 Genome Research Vol.15 No.4

<P>With the availability of the human genome map and fast algorithms for sequence alignment, genome-based EST clustering became a viable method for gene modeling. We developed a novel gene-modeling method, ECgene (Gene modeling by EST Clustering), which combines genome-based EST clustering and the transcript assembly procedure in a coherent and consistent fashion. Specifically, ECgene takes alternative splicing events into consideration. The position of splice sites (i.e., exon-intron boundaries) in the genome map is utilized as the critical information in the whole procedure. Sequences that share any splice sites are grouped together to define an EST cluster in a manner similar to that of the genome-based version of the UniGene algorithm. Transcript assembly is achieved using graph theory that represents the exon connectivity in each cluster as a directed acyclic graph (DAG). Distinct paths along exons correspond to possible gene models encompassing all alternative splicing events. EST sequences in each cluster are subclustered further according to the compatibility with gene structure of each splice variant, and they can be regarded as clone evidence for the corresponding isoform. The reliability of each isoform is assessed from the nature of cluster members and from the minimum number of clones required to reconstruct all exons in the transcript.</P>

문자와 영의 일치 모색에서 하나님의 이름 관련 의제들에 대한 논의: 루돌프 보렌(Rudolf Bohren)의 설교학의 관점에서

조남신(NamShin Cho) 한신대학교 한신신학연구소 2020 신학연구 Vol.57 No.2

A strategy for analysis of soybean genome resequencing data

Soon-Chun Jeong,Namshin Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Soybean is one of the most important crop plants used for seed protein and oil content that has undergone substantial phenotypic and physiological changes during domestication. Thanks to the advent of the next-generation sequencing platforms, genome sequences of many major crop plants including soybean and maize have been unraveled. We have resequenced the genomes of 10 cultivated soybean and 6 accessions of their wild progenitors (Glycine soja) selected from the Korean soybean germplasm to >15 × raw data coverage. We have investigated genome-wide variation patterns in soybean and obtained millions of high-quality single nucleotide polymorphisms (SNPs). Further analyses of the extracted SNPs including population structure analysis, introgressions, linkage disequilibrium, and reduction of diversity are ongoing in order to provide an unprecedented opportunity to finely resolve the domestication history of cultivated soybean. At the same time, we have conducted a comparison study between the Williams 82 soybean reference genome sequence and a genetic map. Here, I will present our current analysis status of the soybean genome resequencing data. Then, I present our recent progresses in the understanding of dynamic genetic features of soybean chromosome revealed by comparison of genetic and sequence-based physical maps in which we have used a portion of our resequencing data to substantiate putative introgression region detected during the construction of a genomewide soybean genetic map.