Purification and Characterization of α-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Hyoung Cchurl Bae,Jong Woo Choi,Myoung Soo Nam 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius CNU27 posessed a high level of α-galactosidase activity. Purified α-galactosi-dase was obtained after sonication of harvested cell pellet followed by DEAE-Sephadex A-50 and Mono Q anion exchangechromatography. The specific activity of the purified enzyme was 8,994 units/mg protein which is 17.09 times higher thanthat in crude extract. The native enzyme was a monomer with a molecular mass of 56,397.1 dalton. The optimum tempera-ture and pH for the enzyme were 40oC and 6.0, respectively. The enzyme was stable between 25 and 50oC. However, α-galactosidase activity was lost rapidly below pH 4.5 and above pH 8.5. The enzyme activity decreased to 6.73% and 4.30%of the original activity by addition of Cu2+ and Hg2+significantly. The enzyme liberated galactose from melibiose, raffinose, and stachyose. The rate of substrates hydrolysiswas measured by HPLC. Raffinose, stachyose and melibiose were completely decomposed after 24 hr at 40oC.

Properties of β-Galactosidase fromLactobacillus salivarius subsp. salivarius Nam27

Hyoung Churl Bae,Gereltuya Renchinkhand,Myoung Soo Nam 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius Nam27 with a high β-galactosidase activity was selected for enzymatic charac-The specific activity of the purified enzyme was 5,312 units/mg. The molecular weight of native monomeric β-galactosi-dase was estimated to be 30,000 dalton (monomer) by the SDS-PAGE. The optimum temperature and optimum pH were50°C and 5.0, respectively. This enzyme was stable between 35 and 55°C. β-Galactosidase activity was lost rapidly abovepH 7.0. But β-galactosidase was more stable at pH 4.0 (acidic conditions). And β-galactosidase activity was lost rapidlyabove 65°C after 10 min incubation. Ca2+ and Zn2+ metal ions enhanced βwhile Cu2+, Fe3+ and Mn2+ lowered β-galactosidase activity by 58.29%, 85.10% and 77.66%, respectively. Other metal ionsdidn’t affect β-galactosidase activity significantly.

Properties of β-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Bae, Hyoung-Churl,Renchinkhand, Gereltuya,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius Nam27 with a high ${\beta}$-galactosidase activity was selected for enzymatic characterization. For purification, cell pellet was disrupted by Bead Beater, by DEAE-Sepharose and Mono-Q chromatography. The specific activity of the purified enzyme was 5,312 units/mg. The molecular weight of native monomeric ${\beta}$-galactosidase was estimated to be 30,000 dalton (monomer) by the SDS-PAGE. The optimum temperature and optimum pH were $50^{\circ}C$ and 5.0, respectively. This enzyme was stable between 35 and $55^{\circ}C$. ${\beta}$-galactosidase activity was lost rapidly above pH 7.0. But ${\beta}$-galactosidase was more stable at pH 4.0 (acidic conditions). And ${\beta}$-galactosidase activity was lost rapidly above $65^{\circ}C$ after 10 min incubation. $Ca^{2+}$ and $Zn^{2+}$ metal ions enhanced ${\beta}$-galactosidase activity by 164.09% and 127.37% while $Cu^{2+}$, $Fe^{3+}$ and $Mn^{2+}$ lowered ${\beta}$-galactosidase activity by 58.29%,85.10% and 77.66% respectively. Other metal ions didn't affect ${\beta}$-galactosidase activity significantly.

Purification and Characterization of α-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Bae, Hyoung-Cchurl,Choi, Jong-Woo,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius CNU27 possessed a high level of ${\alpha}$-galactosidase activity. Purified ${\alpha}$-galactosidase was obtained after sonication of harvested cell pellet followed by DEAE-Sephadex A-50 and Mono Q anion exchange chromatography. The specific activity of the purified enzyme was 8,994 units/mg protein which is 17.09 times higher than that in crude extract. The native enzyme was a monomer with a molecular mass of 56,397.1 dalton. The optimum temperature and pH for the enzyme were $40^{\circ}C$ and 6.0, respectively. The enzyme was stable between 25 and $50^{\circ}C$. However, ${\alpha}$-galactosidase activity was lost rapidly below pH 4.5 and above pH 8.5. The enzyme activity decreased to 6.73% and 4.30% of the original activity by addition of $Cu^{2+}$ and $Hg^{2+}$, respectively. Other metal compounds did not affect the enzyme activity significantly. The enzyme liberated galactose from melibiose, raffinose, and stachyose. The rate of substrates hydrolysis was measured by HPLC. Raffinose, stachyose and melibiose were completely decomposed after 24 hr at $40^{\circ}C$.

五令散의 作用機轉에 대한 考察

南炯臣,趙忠植,金哲中 대전대학교 한의학연구소 2001 한의학연구소 논문집 Vol.10 No.1

Oryoungsan which first recorded in Sanghanron, the clinical medical book consists of treating acute febrile disease according to its change, is one of the frequently used oriental medicines. these days, it has been prescribed in symptoms accompanied by edema mostly. therefore it is easy to consider it as a type of diuretics. In Sanghanron it was originally used in the symptoms of perspiration, decreased urine volume, thirsty, flatulence, these symptoms indicate loss of body fluid and the prescription which orders "taking warm water sufficiently" supports this. On this background, it is supposed that Oryoungsan treats dehydration after providing water and electrolytes. To consider that herbal medicines consisted of Oryoungsan make electrolytes go out of the body, The healing mechanism of dehydration doesn't meet this. Because Oryoungsan was used in condition of fever or in similar condition, it is more resonable to understand that restoration of increasing blood flow to the subcutaneous venous plexus regulating body temperature in febrile condition into body circulation, resulting into maintaining main blood volume and into treating decreased urine volume and thirsty is Oryoungsan's function in the dehydration or febrile condition. That is, symptoms are decreased or disappeared through restoring unbalance of internal body fluid. The other target is pain controls, especially chronic headache, facial pain and trigeminal neuralgia, it is suggested that the funciton of pain control of Oryoungsan is related to 5-HT(5-hydroxytrypamine), nerve transmitter in the endogenous analgesic system. Moreover it is also suggested that Oryoungsan is relate to 5-HT, considering the fact that gastroparesis, a symptom of cyclic vomiting syndrome treated with 5-HTID receptor agonist is similar to the 'bi(??)', symptoms appeared in the Oryoungsan-related disease.

어패럴 CAD System의 활용화 방안 연구(Ⅰ) : Hip Block 의 개발

남윤자,이형숙,조영아 한국의류학회 1993 한국의류학회지 Vol.17 No.4

The Purpose of this study was to develop pattern drafting methods of the hip block and sleeve for female college students of 18 to 26 year-old. The study procedures and results were as follows 1. 313 female college students were measured, 17items were analyzed statisitcally. 2. New hip block and sleeve sloper were developed based on the result of the data analysis and fitting tests. 3. The sensory test was applied to evaluate the new hip block and sleeve sloper for female college student by comparing it with Japanese Bunka method sloper. According to statistical analysis of the result of 40items on the questionnaire, all the items showed significant difference(a∠=0.01) between the two, with the new pattern having higher scores. The new drafting method proved to be better fitted at all 40items, and to be comfortable.

차량 시뮬레이터를 이용한 적응순항제어시스템의 성능 평가

남형권,이운성 국민대학교 생산기술연구소 2003 공학기술논문집 Vol.26 No.-

Adaptive Cruise Control (ACC) system can automatically adjust the headway-time and driver vehicle's speed between a driver vehicle and a preceding vehicle. Driving simulator a is virtual reality tool that allows Driver-In-the-Loop Simulation in a laboratory. This paper addresses driving behavior with Adaptive Cruise Control (ACC) system using driving simulator. Participants with different driving styles have driven the simulator with and without ACC through a highway route. Participants are divided by Driving Style Questionnaire (DSQ)-Focus, Speed and Total DSQ scores. Driving behavior in terms of average headway-time, lateral position of a car, and head and gaze movement has been investigated.

어패럴 CAD System의 활용화 방안 연구(Ⅱ) : 테일러드 쟈켓 설계 과정을 중심으로

남윤자,이형숙,조영아 한국의류학회 1994 한국의류학회지 Vol.18 No.1

The Purpose of this study was to utilize of CAD System in pattern making process for women's apparel. The automation by the use of CAD System provides the higher accuracy and efficiency in pattern making process. AccuMark 300 System was used, for this study. The results from this study were as follows 1. New size spec chart and grading pitch chart were developed based on the data analysis and fitting tests for female college students. 2. New jacket block was developed based on the torso length sloper. 3. Automatic grading of jacket block have been developed by creation and modification of grading rules of block pattern. 4. Pattern Design Systyem(P/D/S) were enabled to be constructed directly form a block pattern by modifications to existing styled pattern. 5. Original master pattern was generated by P/D/S menu option. 6. Production pattern added seam allowance, notchs was generated by P/D/S menu option. 7. Interative maker making process have enabled to save a wide range of time and space. 8. Measurement of garment by P/D/S measuring tools is to utilize in garment costing, quality control.

[논문]실험시설 기초의 진동사용성 분석

최형석,전법규,김남식,정진환 釜山大學校生産技術硏究所 2007 生産技術硏究所論文集 Vol.66 No.-

건물에 작용하는 진동하중은 구조물 자체의 성능에 영향을 미칠 뿐만 아니라 거주하는 사람이나 작업기계 동에 나쁜 영향을 가져올 수 있다. 본 연구에서는 이러한 진동에 대한 영향을 평가하는 국내외의 기준에 대하여 고찰하고 다양한 하중과 주파수 대역에서 실험이 가능한 대형지진모사 실험센터에 대한 진동영향 평가를 수행하여 사용성 저하 여부를 확인하였다.

석·골재의 분류 및 패턴 분류

남부희,박민현,김형배 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

우리는 DWT를 적용하요 석ㆍ골재의 패턴 분류와 인식을 위한 테이타베이스를 구축하였다. 우선, 우리는 이미지의 각 row 벡터를 DWT를 수행하여 각 row 벡터마다 특징값을 얻는다. 그리고나서 같은 방법으로 row 벡터의 특징값을 이미지전체의 특징 값을 얻을 때가지 DWT를 반복해서 수행한다. 그런 다음 우리는 이미지의 히스트그램을 얻는다. 특징 값과 히스토그램은 이미지의 특징을 나타낸다. CCD 카메라로 새로운 이미지를 얻었을 때 우리는 먼저 특징 값과 히스트그램을 얻는 과정을 수행한다. 그 특징 값을 가지고 패턴 원형을 찾기 위해 저장된 특징 값과 비교하여 비슷한 값들을 찾아낸다. 그리고 입력 데이터의 히스트그램을 가지고 이전 과정에서 찾아낸 이미지들의 히스트그램과 비교하여 가장 유사한 패턴 원형을 찾아낸다. 이 방법은 석ㆍ골재의 자동분류를 할 수 있도록 하여 생상선 향상과 비용절감을 가능하게 한다. We built some database of stone and aggregate resources for classification and pattern recognition using DWT(discrete wavelet transform). First, we perform one dimensional DWT for each row of the image and obtain one value for each row of image. Then we perform again the same DWT for them to get a feature value. Next, we evaluate the histogram of input image data. The feature value and histogram represent the feature of image data. when an image data achieved by CCD camera, we perform same process again to get a feature value and a histogram. the feature value is compared with stored feature values to choose candidates. And then the histogram of input data is compared with some histogram value of candidates to find target image. this method can be adapted to automation of manufacturing of stone and aggregate resources for high quality low cost products.