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Transduction of the Wild-type polA Gene of Escherichia coli K-12 in a ColE1-Derived Mini-Mu Plasmid
Parduez, Nagy-Gyorgy,Choi, Yong-Keel,Chung, Young-Sup The Microbiological Society of Korea 1992 미생물학회지 Vol.30 No.2
Teh $polA^{+}$ gene can be transducted in a multicopy mini-Mu plasmid, but not cloned because the product of this gene is lethal when overproduced. Although, we obtained one surviving cell, in which the ColEl-derived mini-Mu plasmid suffered a spontaneous deletion exactly at the region where the $polA^{+}$ gene was cloned. The $PolA^{+}$ unstream flanking sequence containing the promoter and pribnow-box was delected in vivo ; consequently this gene is not able to be expressed.