Efficient White-light Fluorescent/Phosphorescent Complex Electrolluminescent Devices

Nok Hyun Ju,Tae-Ho Kim,Hang Ken Lee,O Ok Park 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

Identification of a Domain in Yeast Chitin Synthase 3 Interacting with Chitin Synthase 4 by Two-Hybrid Analysis

( Hyun Sook Park ),( Shin Jung Choi ),( Nok Hyun Park ),( Chi Hwa Kim ),( Sung Uk Kim ),( Won Ja Choi ) 한국미생물생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.6

Comparative proteomic analysis of human follicular dermal papilla cells and fibroblasts

Chong Hyun Won,Chae Hwa Kim,Oh Sang Kwon,Yong Jung Kang,Jeon Hae Chan,Hyeon Gyeong Yoo,Jin Ho Chung,Kyu Han Kim,Hee Chul Eun,Kun Cho,Jong Soon Choi,Won Seok Park,Nok Hyun Park 대한피부과학회 2009 대한피부과학회 학술발표대회집 Vol.61 No.2

Ginsenoside composition of Panax ginseng flower extracts obtained using different high hydrostatic pressure extraction conditions

Kim, Hyun Soo,Kim, Gyu Ri,Kim, Donghyun,Zhang, Cheng-Yi,Lee, Eun-Soo,Park, Nok Hyun,Park, Junseong,Lee, Chang Seok,Shin, Moon Sam The Korean Society of Plant Biotechnology 2019 식물생명공학회지 Vol.46 No.1

Ginsenosides are active constituents of ginseng (Panax ginseng) that have possible anti-aging, physiological and pharmacological activities, such as anti-cancer and anti-inflammatory effects. Although the ginseng root is generally used more often than the aerial parts for medicinal purposes, the flowers also contain numerous ginsenosides, including Rb2, Rc, Rd, Re and Rg1. Therefore, an extract from the flowers of the P. ginseng could have the pharmacological efficacy of bioactive compounds including ginsenosides. The high hydrostatic pressure extraction (HHPE) is a method that is used for the efficient extraction of bioactive compounds from plant materials. In this study, we compared the yield of ginsenosides from ginseng flowers under different conditions of extraction pressure and time of HHPE. The results indicate that the total yield of the ginsenosides improved as the pressure increased from 0.1 to 80 MPa and treatment duration increased to 24 hours. In addition, the ginsenoside extracts from HHPE at 80 MPa, which possessed a higher total ginsenoside concentration, decreased the viability of the primary human epidermal keratinocytes (HEKs) significantly than the ginsenoside extracts from HHPE at 0.1 MPa. Collectively, we found that the method of HHPE that was performed for 24 hours at 80 MPa showed the highest yield of ginsenosides from the flowers of P. ginseng. In addition, our study provides a foundation for the efficient extraction of ginsenosides, which had a potent bioactivity, from flowers of P. ginseng through HHPE.

Comparative secretome analysis of human follicular dermal papilla cells and fibroblasts using shotgun proteomics

( Chong Hyun Won ),( Oh Sang Kwon ),( Yong Jung Kang ),( Hyeon Gyeong Yoo ),( Dong Hun Lee ),( Jin Ho Chung ),( Kyu Han Kim ),( Won Seok Park ),( Nok Hyun Park ),( Kun Cho ),( Sang Oh Kwon ),( Jong So 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.4

The dermal papilla cells (DPCs) of hair follicles are known to secrete paracrine factors for follicular cells. Shotgun proteomic analysis was performed to compare the expression profiles of the secretomes of human DPCs and dermal fibroblasts (DFs). In this study, the proteins secreted by DPCs and matched DFs were analyzed by 1DE/LTQ FTICR MS/MS, semi-quantitatively determined using emPAI mole percent values and then characterized using protein interaction network analysis. Among the 1,271 and 1,188 proteins identified in DFs and DPCs, respectively, 1,529 were further analyzed using the Ingenuity Pathway Analysis tool. We identified 28 DPC-specific extracellular matrix proteins including transporters (ECM1, A2M), enzymes (LOX, PON2), and peptidases (C3, C1R). The biochemically- validated DPC-specific proteins included thrombospondin 1 (THBS1), an insulin-like growth factor binding protein3 (IGFBP3), and, of particular interest, an integrin beta1 subunit (ITGB1) as a key network core protein. Using the shotgun proteomic technique and network analysis, we selected ITGB1, IGFBP3, and THBS1 as being possible hair-growth modulating protein biomarkers. [BMB reports 2012; 45(4): 253-258]

장양(藏釀) 기술이 사용된 전통 고숙성 된장에서 유래한 o-Dihydroxyisoflavone 유도체의 생리활성

김동현 ( Dong Hyun Kim ),박준성 ( Jun Seong Park ),박녹현 ( Nok Hyun Park ),문은정 ( Eun Jeong Moon ),유선혜 ( Sun Hye Yu ),김덕희 ( Duck Hee Kim ),김한곤 ( Han Kon Kim ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.3

된장은 한국의 독특한 발효 식품이며 장양이라 불리는 전통적인 제조 방법에 의해 제조된다. 이에 본 연구에서는 고숙성 된장에서 새롭게 생성된 성분과 그것의 생리활성을 측정하였다. 5년 숙성된 된장으로부터 새로운 o-dihydroxyisoflavone인 7,3',4'-trihydroxyisoflavone과 기존에 알려진 두 가지의 o-dihydroxyisoflavone을 각각 분리하여 다른 isoflavone과의 항산화 효과 및 미백효과를 비교 측정하였다. Diphenyl-1-picrylhydrazyl (DPPH) 활성 저해 효과는 7,8,4'-trihydroxyisoflavone (compound 1), 7,3',4'-trihydroxyisoflavone (compound 2) 그리고 6,7,4'-trihydroxyisoflavone (compopund 3)은 각각 21.5 ± 0.2, 28.7 ± 0.4 그리고 32.6 ± 0.6 (IC₅₀)을 나타낸 반면 daidzein은 이들에 비해 약한 DPPH 활성 저해 효과를 나타냈다. Superoxide 소거 효과는 L-ascorbic acid에 비해 compound 1 (IC₅₀ = 18.10 ± 0.2 μM)과 2 (IC₅₀ = 10.54 ± 0.4 μM)가 보다 효과적인 반면 compound 3과 daidzein은 이들에 비해 낮은 활성을 나타냈다. 또한 melan-a cells에서 o-dihydroxyisoflavone 유도체들의 티로시나제 활성과 멜라닌 생성을 비교 측정하였다. 특히 Compound 1 (IC₅₀ = 11.21 ± 0.2 μM), 2 (IC₅₀ = 5.23 ± 0.6 μM)의 경우 compound 3과 daidzein에 비해 티로시나제 활성을 억제하는데 효과가 있었으며 또한 멜라닌 생성을 50 % 억제할 때의 결과에서 이들 화합물은 각각 12.23 ± 0.7 μM (1), 7.83 ± 0.7 μM (2)과 같이 멜라닌 생성 억제 효능을 나타내었다. 따라서 고숙성 된장에서 유래한 7,3',4'-trihydroxyisoflavone은 항산화 및 미백효과를 가지는 화장품 소재로써 사용 가능하리라 판단된다. Doenjang (Korean fermented soybean paste) is a unique fermented food in Korea. It has been traditionally manufactured from soybeans, by Jang Yang process. We focused on the newly formed compound in highly aged Doenjang and its biological activity. One new o-dihydroxyisoflavone, 7,3',4'-trihydroxyisoflavone and two known o-dihydroxyisoflavone derivatives were isolated from 5-year-old Doenjang and evaluated as potent antioxidant and whitening effect by comparing with other known isoflavone. 7,8,4'-Trihydroxyisoflavone (compound 1), 7,3',4'-trihydroxyisoflavone (compound 2) and 6,7,4'-trihydroxyisoflavone (compound 3) inhibited DPPH (diphenyl- 1-picrylhydrazyl) formation by 50 % at a concentration of 21.5 ± 0.2, 28.7 ± 0.4 and 32.6 ± 0.6 (IC₅₀) respectively, whereas daidzein showed weak DPPH radical scavenging activity. In superoxide scavenging effect were measured in one assay. Compound 1 (IC₅₀ = 18.10 ± 0.2 μM) and 2 (IC50 = 10.54 ± 0.4 μM) show significant inhibitory activity and greater effect than L-ascorbic acid. But compound 3 and daidzein showed lower inhibition activity. Also, o-dihydroxyisoflavone derivatives evaluated as potent inhibitors on tyrosinase activity and melanin formation in melan-a cells. Compound 1 (IC₅₀ = 11.21 ± 0.2 μM), compound 2 (IC₅₀ = 5.23 ± 0.6 μM) exhibited significant inhibitory effect on tyrosinase activity. Furthermore, those compounds are significantly suppressed the cellular melanin formation by 50 % at a concentration of 12.23 ± 0.7 μM (1) and 7.83 ± 0.7 μM (2). This result suggests that 7,3',4'- trihydroxyisoflavone from highly aged Doenjang could be used as an active ingredient for cosmetics.

어머니의 양육태도가 유아의 문제행동에 미치는 영향

김녹현(Kim, Nok-Hyun),정영주(Jeong, Yeong-Ju),차선희(Cha, Sun-Hee) 한국복지행정학회 2014 복지행정논총 Vol.24 No.2

본 연구는 어머니의 양육태도가 유아의 문제행동에 미치는 영향을 고찰하기 위하여 경상북도 상주시에 소재한 국·공립 유치원 및 사립유치원의 유아 어머니 211명을 대상으로 분석하였다. 어머니의 최종학력과 아버지의 최종학력, 가족의 한 달 수입의 하위영역에서는 유의미한 차이를 나타났고, 어머니의 양육태도와 유아의 문제행동의 관계에서 어머니의 양육태도 중 애정적 태도가 상관관계가 높은 것으로 나타났으며, 어머니의 양육태도에 따른 유아의 문제행동에 대한 영향은 어머니의 거부적 태도가 높을수록 유아의 문제행동은 증가한다고 할 수 있다. 또한 어머니의 양육태도는 유아의 문제행동과 관계가 있으며, 지대한 영향을 미치는 것을 알 수 있다. 유아의 모든 행동은 그가 어떻게 키워졌느냐 하는 부모의 양육태도의 결과라고 볼 수 있을 만큼 부모의 양육태도가 인격형성에 지대한 영향을 미친다는 것은 아무리 강조해도 지나치지 않는다. 따라서 이러한 요인을 분석하여 유아의 환경과 특성을 이해하고 문제행동에 따른 예방, 개입 등의 방법들에 대한 논의를 하고자 한다. The aim of this treatise is to study an effect of the mother’s nurture attitude on the children problematic behavior. Research target area is Kyungbuk, Sangju city, target people are 211 the children mothers who have the enrolled infants in the national, public and private kindergarten in that area, and results of study are as followings. At first, the results of the study are as followings; mother’s education, father’s education, and the family’s monthly income are statistically significant, The correlation of the mother’s affective nurture attitude and infant’s problematic behavior is very high. The higher the mother’s rejective attitude, the more the infant’s problematic behavior in the effect of the mother’s nurture attitude on the infant’s problematic behavior. Also, we can find that the mother’s nurture attitude is related to and has a serious effect on the infants problematic behavior. Because all infants behaviors are the results of the parent’s nurture attitudes that how they were brought up, we cannot emphasize the serious effect of the parents’ nurture attitudes on infants’ personality formation. Therefore, we can conclude as followings. we should understand infant environment and characteristics by analyzing the above-mentioned factors and should discuss the method to prevent and intervene in the problematic behavior.

모션 정보를 이용한 딥러닝 기반 맞춤형 재활운동

최대현(Dae-hyun Choi),김석현(Seok-Hyun Kim),정녹규(Nok-Gyu Jeong),송지우(Ji-Woo Song),김민규(Min-Gyu Kim),곽근창 대한전기학회 2022 대한전기학회 워크샵 Vol.2022 No.7

경상북도 장애인거주시설 생활재활교사의 직무요인이 소진에 미치는 영향

김녹현(Kim, Nok-Hyun),김은숙(Kwon, Oh-Sook) 한국복지행정학회 2013 복지행정논총 Vol.23 No.2

본 연구는 장애인 거주시설 종사자 중 생활재활교사의 소진에 미치는 영향을 고찰하기 위하여 경북의 재활교사 254명을 대상으로 조사 분석한 결과를 중심으로 요약한 결과는 다음과 같다. 첫째, 직무환경개선을 위해 근무교대 방식을 근로조건에 부합되게 탄력적으로 조정하며 신체적, 정신적 소진이 일어나지 않도록 휴식과 휴가를 적절히 지원할 수 있도록 조직차원에서 조정과 배려가 필요하다. 둘째, 직무에 대한 과중한 분담을 줄이기 위한 업무의 전산화와 다양한 업무의 매뉴얼화로 명료화하고 직원들의 개개인의 능력과 전문적인 기술을 발휘할 수 있도록 업무의 자율성 보장과 적절한 인센티브와 함께 각종 업무의 공정성을 기하는 등 인간적인 관리경영으로 이직의도를 줄이고 소진을 경감시켜야 할 것이다. 셋째, 사회적 인지도를 높이고 생활재활교사 스스로가 직업에 대한 자긍심향상을 위해 사회적 관심촉구와 함께 직원후생복지와 사회복지종사자의 처우개선과 인식개선을 위한 사업들을 기관과 협회차원에서 조직적으로 꾸준히 노력하고 시행해야 할 것이다. 넷째, 상사와의 관계개선을 위해 기관장은 변혁적 리더십을 가지고 직원들의 사기진작과 상호 신뢰와 우호적이고 협력적인 분위기를 조성하여야 하며 직장공동체 의식을 심어주어 클라이언트와도 좋은 관계를 유지할 수 있도록 적극적인 자세를 취해야 할 것이다. 다섯째, 직원후생복지차원에서 직원들에게 적절한 포상휴가제도 및 자기개발을 위한 워크숍이나, 정기적인 교육훈련과 재교육에 대한 적절한 지원으로 직원들이 자기발전과 함께 긍지와 보람을 가지고 직무에 종사할 수 있는 환경을 조성해야 할 것이다. The aim of this treatise is to study the factors affecting the burn-out of living rehabilitation teachers who live in the disable living facilities. Research target area is Kyungbuk area, target people are 254 rehabilitation teachers who live in the disable living facilities in that area, and results of study are as followings. First, the results of the study are as followings; sex, age, marriage, education, religion, occupation period, and occupation area are statistically significant, but major, occupation motive, facility type, occupation type, and yearly income didn t show statistically significant relations with burn-out. Second, job environment, staff fringe benefit, social cognition, job equitability, and intention of job change in job environment showed significantly meaningful correlations with emotional burn out, individual achievement deficiency, client s impersonalization in burn-out subcategory. And social cognition showed higher correlation with individual achievement in comparison with other factors, and job environment factors showed the correlation with client s impersonalization and emotional burnout. Third, wage, relation with direct superior, job itself, relation with clients in job satisfaction category showed significantly meaningful correlations with emotional burn out. But low satisfaction in job itself showed higher correlations with emotional burn-out, individual achievement deficiency by comparison with other factors, relation with direct superior showed higher relation with client s impersonalization.

Interacting Domain Between Yeast Chitin Synthase 3 and Chitin Synthase 4 is Involved in Biogenesis of Chitin Ring, but not for Cell Wall Chitin

( Shin Jung Choi ),( Nok Hyun Park ),( Hyun Sook Park ),( Mee Hyun Park ),( Jee Eun Woo ),( Won Ja Choi ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.2