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High-yield biosynthesis of luteolin glucosides by amylosucrase from Deinococcus geothermalis
Se-Won Jang,Dong-HoSeo,Byung-HooLee,Nam-InBaek,Cheon-SeokPark 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Luteolin(3’,4’,5,7-hydroxy flavone) is a flavone aglycone known to have anti-inflammatory, antioxidant and anti-cancer effects, but it is limited in pharmaceutical material due to low water-solubility. Therefore, the aim of study is to synthesize luteolin glycoside (LG) for increasing water-solubility. The LG was synthesized by the transglycosylation reaction of amylosucrase of Deinococcus geothermalis (DGAS) using luteolin and sucrose as an acceptor and a donor, respectively. The maximum yield of the LG product was established to be 89.3 % with a 1:7.16 molar ratio of donor and acceptor molecules, in 50 mM Tris-HCl buffer (pH 7) at 37°C for 24 hr and the results of HPLC showed that only one transglycosylation product was detected. The structural analyses using 13C and 1HNMR proved that the transglycosylated product was luteolin-4'-O-glucoside.