Normal and Neoplastic Regeneration of the Human Uterine Cervix Epithelium: Ultrastructure of the Normal Cell Types

NAGL,Walter 대구대학교 특수교육재활과학연구소 1978 再活科學硏究 Vol.1 No.1

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Endometrial cancer risk and survival by tumor MMR status

Christina M. Nagle,Christina M. Nagle,Tracy A. O'Mara,Yen Tan,Daniel D. Buchanan,Andreas Obermair,Penny Blomfield,Michael A. Quinn,Penelope M. Webb,Amanda B. Spurdle,Australian Endometrial Cancer Stud 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.3

Objective: The risk of developing endometrial cancer (EC) and/or survival following a diagnosis of EC might differ by tumor DNA mismatch repair (MMR) status. We assessed the association between tumor MMR status (classified as MMR-proficient, somatic MMR-deficient, germline MMR-deficient) and the risk of developing EC and survival following a diagnosis of EC. Methods: We analyzed data from women who participated in the Australian National Endometrial Cancer Study (ANECS) conducted between 2005 and 2007. Risk analyses (698 cases/691 population controls) utilized sociodemographic and lifestyle information obtained from telephone interviews at recruitment. For survival analyses (728 cases), patients' clinical data was abstracted from medical records, and survival data were obtained via linkage with the Australian National Death Index. We used logistic regression analysis to evaluate the associations between tumor MMR status and EC risk, and proportional hazards models to perform survival analyses with adjustment of known prognostic factors. Results: Established risk factors for EC did not differ significantly by tumor MMR status. In analyses including all EC subtypes, overall and EC-specific survival did not differ by tumor MMR status. Among women with the most common endometrioid subtype, EC-specific survival was worse for women with somatic MMR-deficient EC compared to women with MMR-proficient EC (hazard ratio [HR]=2.18; 95% confidence interval [CI]=1.19–4.01). Conclusion: The risk of EC is not associated with MMR status. Accurate separation of germline from somatic causes of MMR deficiency suggests that patients with endometrioid subtype somatic MMR-deficient tumors have poorer EC-specific survival than those with MMR-proficient tumors, after accounting for other prognostic factors.

Normal and Neoplastic Regeneration of the Human Uterine Cervix Epithelium: Ultrastructure of the Normal Cell Types

NAGL,Walter 大邱大學校 再活科學硏究所 1978 再活科學 Vol.1 No.1

The ultrastructure of the cervical gland epithelium is investigated since some 20 years (Doughtery and Low, 1958; Hashimoto and Jorisato, 1959; Doughtery, 1961; Chapman et at., 1964; Stegner and Beltermann, 1969; Hiersche, 1970; Philipp, 1973). There is, however, sti]1 some controversy on which cell type of the cervix is responsable for the regeneration of the gland epithelium, when it is locally destroyed in the consequence of coitus(Singer, 1976), or lysed after complete secretion. While some authors are sure that the epithelium regenerates by mitotic division (Read and Copplesson, 1964; Stegner and Beltermann, 1969), other authors suggest that undifferentiated cells, the so-called basal or reserve cells are stem-cells for epithelium regeneration and differentiate into gland cells (Fettig and Oehlert, 1964; Rosenthal and Hellmann, 1952). As the normal capacity of cells to divide is of importance for the understanding of the origin of hypoplasias and the frequently occurring cervix carcinomas, 1 decided to study the organization, regeneration, and differentiation pathways of the human uterine cervix at the ultrastructural level. In this report the various cell types are analysed for their normal cytoplasmic and nuclearstructures. The differentiation of the squamous epithelium is not included in this study.

Simultaneous Detection of Tartrazine-Sunset Yellow in Food Samples Using Bioxide/Carbon Paste Microcomposite with Lanthanum and Titanium

Nagles, Edgar,Ceroni, Mario,Hurtado, John The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.4

This report describes the simultaneous detection of the two dyes most commonly used in food, tartrazine (TZ) and sunset yellow (SY), based on a microcomposite of carbon paste decorated with La₂O₃ and TiO₂. Anodic currents for SY-TZ were observed at 0.89-1.21 V by cyclic voltammetry (CV) separated with a ΔV of 0.32 V. The increased anodic peak currents compared to that of the unmodified carbon paste electrode were almost 50 and 41% for SY-TZ, respectively. The detection limits with the optimal amount of La₂O₃-TiO₂ were 0.02 and 0.03 µmol/L, respectively. The relative standard deviation (RSD) based on fifty measurements was less than 3%. The versatility and novelty of the sensor were tested with food samples containing these substances and other substances.

Free Range Hens Use the Range More When the Outdoor Environment Is Enriched

Nagle, T.A.D.,Glatz, P.C. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.4

To evaluate the role of using forage, shade and shelterbelts in attracting birds into the range, three trials were undertaken with free range layers both on a research facility and on commercial farms. Each of the trials on the free range research facility in South Australia used a total of 120 laying hens (Hyline Brown). Birds were housed in an eco-shelter which had 6 internal pens of equal size with a free range area adjoining the shelter. The on-farm trials were undertaken on commercial free range layer farms in the Darling Downs in Southeast Queensland with bird numbers on farms ranging from 2,000-6,800 hens. The first research trial examined the role of shaded areas in the range; the second trial examined the role of forage and the third trial examined the influence of shelterbelts in the range. These treatments were compared to a free range area with no enrichment. Aggressive feather pecking was only observed on a few occasions in all of the trials due to the low bird numbers housed. Enriching the free range environment attracted more birds into the range. Shaded areas were used by 18% of the hens with a tendency (p = 0.07) for more hens to be in the paddock. When forage was provided in paddocks more control birds (55%) were observed in the range in morning than in the afternoon (30%) while for the forage treatments 45% of the birds were in the range both during the morning and afternoon. When shelterbelts were provided there was a significantly (p<0.05) higher % of birds in the range (43% vs. 24%) and greater numbers of birds were observed in areas further away from the poultry house. The results from the on-farm trials mirrored the research trials. Overall 3 times more hens used the shaded areas than the non shaded areas, with slightly more using the shade in the morning than in the afternoon. As the environmental temperature increased the number of birds using the outdoor shade also increased. Overall 17 times more hens used the shelterbelt areas than the control areas, with slightly more using the shelterbelts in the afternoon than in the morning. Approximately 17 times more birds used the forage areas compared to the control area in the corresponding range. There were 8 times more birds using a hay bale enriched area compared to the area with no hay bales. The use of forage sources (including hay bales) were the most successful method on-farm to attract birds into the range followed by shelterbelts and artificial shade. Free range egg farmers are encouraged to provide pasture, shaded areas and shelterbelts to attract birds into the free range.

Ultrastructure of the Polytene Nuclei in the Embryo-Suspensor of the Bean, Phaseolus coccineus : Toothing of Cytoplasm and Nucleoplasm

NAGL, WALTER 韓國自然史博物學會 1976 生物과 自然 Vol.6 No.-

The polytene nuclei of the embryo-suspensor in Phaseolus coccineus show numerous deep cytoplasmic invaginations, which highly increase the nucleo-cytoplasmic interface. The telomeric regions of the polytene chromosomes are dispersed into small chromatin patches, some of which are attached to the nuclear envelope. Ribonucleoprotein granules are frequently found between these chromatin patches. The nuclear pore frequency is uncommonly high (about 50 pores/μm^2). These findings indicate high nucleo-cytoplasmic exchange activity in the suspensor cells, and may be an explanation for the impossibility to obtain a purified nuclear fraction from the suspensor by centrifugation.

Preparation of silicon nanoball encapsulated with graphene shell by CVD and electroless plating process

김희진,황태연,강근원,Jeanine Pichler-Nagl,소대섭,박성진,허훈 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.50 No.-

Carbon materials such as graphite have been widely used as the anode materials of secondary batteries. Inspite of good stability, they are limited by low capacity. Silicon has a 4 times higher capacity than anyother carbon material. But it cannot be used, because it has a low stability caused by the volume expansion. In this study, we fabricated a silicon nanoball encapsulated with graphene (Si-GB) to overcome thementioned problems. In order to form a core/shell structured graphene encapsulated silicon nanoball,nickel was coated on the surface of a silicon nanoball by electroless plating method. Then, a graphene layerwas synthesized on the surface of the nickel shell by a CVD process. We were able to make a Si-GB byetching the nickel layer. The Si-GBs were formed with a spherical void between the silicon particle and thegraphene layer, by which it has a better safety against to volumetric change of anode during lithiation/delitiation of repeated charging–discharging in secondary batteries. Morphology of the grapheneencapsulated silicon nanoball was observed by thefield emission scanning electron microscope (FESEM)and thefield emission transmission electron microscope (FETEM) tofind core–shell structured nanoball. Spherical structure of graphene encapsulated silicon nanoball was investigated by the Raman, the X-rayPhotoelectron Spectroscopy to identify graphene layer on the surface of the inner silicon core. The charge–discharge performance of the Li-ion battery using Si-GB was investigated by the battery cycler system.

Preparation of silicon nanoball encapsulated with graphene shell by CVD and electroless plating process

Kim, H.,Hwang, T.,Kang, K.,Pichler-Nagl, J.,So, D.S.,Park, S.,Huh, H. THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2017 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.50 No.-

<P>Carbon materials such as graphite have been widely used as the anode materials of secondary batteries. Inspite of good stability, they are limited by low capacity. Silicon has a 4 times higher capacity than any other carbon material. But it cannot be used, because it has a low stability caused by the volume expansion. In this study, we fabricated a silicon nanoball encapsulated with graphene (Si-GB) to overcome the mentioned problems. In order to form a core/shell structured graphene encapsulated silicon nanoball, nickel was coated on the surface of a silicon nanoball by electroless plating method. Then, a graphene layer was synthesized on the surface of the nickel shell by a CVD process. We were able to make a Si-GB by etching the nickel layer. The Si-GBs were formed with a spherical void between the silicon particle and the graphene layer, by which it has a better safety against to volumetric change of anode during lithiation/delitiation of repeated charging discharging in secondary batteries. Morphology of the graphene encapsulated silicon nanoball was observed by the field emission scanning electron microscope (FESEM) and the field emission transmission electron microscope (FETEM) to find core shell structured nanoball. Spherical structure of graphene encapsulated silicon nanoball was investigated by the Raman, the X-ray Photoelectron Spectroscopy to identify graphene layer on the surface of the inner silicon core. The charge discharge performance of the Li-ion battery using Si-GB was investigated by the battery cycler system. (C) 2017 Published by Elsevier B.V. on behalf of The Korean Society of Industrial and Engineering Chemistry.</P>