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순무(Brassica campestris ssp. rapa) 뿌리로부터 지질화합물의 분리 및 동정
방면호,오영준,유종수,한민우,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H_(2)O분획으로 나누었으며, EtOAc분획에 대해 silica gel column chromatography를 실시하여 3종의 지질화합물을 분리 정제하였다. ^(1)H-NMR, ^(13)C-NMR, DEPT spectrum 및 Mass spectrum등을 통하여 palmitic acid methyl ester(compound 1), linolenic acid methyl ester(compound 2), linoleic acid methyl ester(compound 3)으로 구조를 결정하였다. In order to isolate and identify the active compound from the Brassica campestris ssp rapa, the roots and the root skin were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel column chromatographies. From the result of spectral data including NMR and MS, the chemical structures of the compound were determined as palmitic acid methyl ester (compound 1), linolenic acid methyl ester (compound 2), linoleic acid methyl ester (compound 3).
유종수,방면호,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
강화 특화작목의 건강기능성 식품 제품화의 가능성을 타진하고 재배작물의 표준화 연구를 위해 사자발쑥, 싸주아리쑥 그리고 순무에 대해 총 폴리페놀과 총 플라보노이드 함량을 측정하였다. 총 폴리페놀 함량은 사자발쑥이 9.5mg/g, 싸주아리쑥이 7.4mg/g, 순무가 1.8mg/g으로 총 플라보노이드 함량은 사자발쑥이 4.3mg/g, 싸주아리쑥이 3.6mg/g, 순무가 0.4mg/g으로 확인 되었다. 또한 강화 약쑥의 유효지표성분인 eupatilin과 jaceosidin이 사자발쑥에는 각각 208mg/100g, 118mg/100g, 싸주아리쑥에는 199mg/100g, 111mg/100g 함유된 것을 HPLC를 통해 확인하였다. This study was conducted to investigate the contents of total polyphenol and total flavonoid of three Ganghwa indigenous crops, two varieties of Artemisia princeps Pampanini (Sajabalssuk, Sajuarissuk) and Brassica campestris ssp rapa. Total polyphenol and total flavonoid contents of Sajabalssuk were 9.5 and 4.3 mg/g, those of Sajuarissuk were 7.4 and 3.6 mg/g, those of B. campestris ssp rapa were 1.8 and 0.4 mg/g, respectively. The contents of eupatilin and jaceosidin in A. princeps Pampanini which were calculated by HPLC, were determined 208 mg/100g, 118 mg/100g in Sajabalssuk and 199 mg/100g, 111 mg/100g in Sajuarissuk, respectively.
싸주아리쑥(Artemisia herba)으로부터 phytol의 분리
유종수,방면호,정해곤,정태숙,최명숙,이경태,백남인 경희대학교식량자원개발연구소 2006 硏究論文集 Vol.25 No.-
싸주아리쑥 지상부의 80% MeOH 추출물로부터 얻어진 EtOAc 분획물에 대하여 silica gel 및 ODS column chromatography를 반복하여 diterpene을 분리하였다. 화합물의 화학구조는, NMR, MS 및 IR 등의 스펙트럼을 해석하여 phytol로 동정하였다. The aerial part of Artemisia herba was extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, a terpenoid was isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structure of the compound was determined as phytol.
식용식물자원으로부터 활성물질의 탐색 XXII. 순무(Brassica campestris ssp rapa) 뿌리로부터 인돌 화합물의 분리 및 hACAT 저해 활성
방면호 ( Myun Ho Bang ),이대영 ( Dae Young Lee ),오영준 ( Young Jun Oh ),한민우 ( Min Woo Han ),양혜정 ( Hye Joung Yang ),정해곤 ( Hae Gon Chung ),정태숙 ( Yae Sook Jeong ),이경태 ( Kyung Tae Lee ),최명숙 ( Myung Sook Choi ),백남 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.1
식용식물자원으로부터 활성물질의 탐색-XXII. 사자발쑥 (Artemisia princeps PAMPANINI)의 지상부로부터 Triterpenoid의 분리
방면호 ( Myun Ho Bang ),조진경 ( Jin Gyeong Cho ),송명종 ( Myoung Chong Song ),이대영 ( Dae Young Lee ),한민우 ( Min Woo Han ),정해곤 ( Hae Gon Chung ),정태숙 ( Tae Sook Jeong ),이경태 ( Kyung Tae Lee ),최명숙 ( Myung Sook Choi 한국응용생명화학회(구 한국농화학회) 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.3
식용식물자원으로부터 활성물질의 탐색 16. 사자발쑥(Artemisia herba)의 전초로부터 sterol 화합물의 분리
방면호 ( Myun Ho Bang ),정해곤 ( Hae Gon Chung ),송명종 ( Myoung Chong Song ),유종수 ( Jong Su Yoo ),정선아 ( Sun A Chung ),이대영 ( Dae Young Lee ),김세영 ( Se Young Kim ),정태숙 ( Tae Sook Jeong ),이경태 ( Kyung Tae Lee ),최명숙 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.2
차상면,최희윤,엄기일,김잉곤,류재만 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4
Various surgical method have been used in an attempt to achieve a normal - looking growth of hair in scalp alopecia. A variety of flaps have been applied both for primary closure and for secondary repair, but flaps very seldom result in the correct direction of hair growth. In case of more extensive scalp alopecia, the shifting of flaps would only mean to move one denuded area to another, owing to inelasticity of the scalp tissue. Split-skin grafts still have to be used in such cases, which will remain hairless. Nowadays, tissue expansion became the one of the most popular technique in the field of plastic surgery since it k was first introduced by Dr. Neumann, who attempted an ear reconstruction using a latex ballon with a valve and a connector tube lying outside the skin in 1957 and Radovan, who introduced more safer and controllable soft tissue expansion. In this paper, 15 cases of scalp alopecia were operated by using tissue expansion from September, 1988 to September, 1990. All cases were successfully reconstructed with 4 major complication. This complications were hematoma, infection, wound separation, tissue necrosis. Here, presentative case reports are presented and the management and prevention of complications are discussed.