A Soviet Journey: A Critical Annotated Edition by Alex La Guma (review)

Myra Ann Houser 한국외국어대학교(글로벌캠퍼스) 러시아연구소 2019 REGION Vol.8 No.1

Transference rind Counter-Transference as Manifested in Graphic Productions Re-Visited

Myra F,Levick 한국미술치료학회 2000 한국미술치료학회 학술발표대회 Vol.7 No.-

기조학술발표 1

호주의 휴가제도

Myra Hamilton 한국노동연구원 2017 국제노동브리프 Vol.15 No.7

Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows

Hosmillo, Myra,Jeong, Young-Ju,Kim, Hyun-Jeong,Park, Jun-Gyu,Nayak, Mukti Kant,Alfajaro, Mia Madel,Collantes, Therese Marie,Park, Su-Jin,Ikuta, Kazuyoshi,Yunoki, Mikihiro,Kang, Mun-Il,Park, Sang-Ik,Ch Springer-Verlag 2010 Archives of virology Vol.155 No.7

Sapovirus Translation Requires an Interaction between VPg and the Cap Binding Protein eIF4E

Hosmillo, Myra,Chaudhry, Yasmin,Kim, Deok-Song,Goodfellow, Ian,Cho, Kyoung-Oh American Society for Microbiology 2014 Journal of virology Vol.88 No.21

<P>Sapoviruses of the <I>Caliciviridae</I> family of small RNA viruses are emerging pathogens that cause gastroenteritis in humans and animals. Molecular studies on human sapovirus have been hampered due to the lack of a cell culture system. In contrast, porcine sapovirus (PSaV) can be grown in cell culture, making it a suitable model for understanding the infectious cycle of sapoviruses and the related enteric caliciviruses. Caliciviruses are known to use a novel mechanism of protein synthesis that relies on the interaction of cellular translation initiation factors with the virus genome-encoded viral protein genome (VPg) protein, which is covalently linked to the 5′ end of the viral genome. Using PSaV as a representative member of the <I>Sapovirus</I> genus, we characterized the role of the viral VPg protein in sapovirus translation. As observed for other caliciviruses, the PSaV genome was found to be covalently linked to VPg, and this linkage was required for the translation and the infectivity of viral RNA. The PSaV VPg protein was associated with the 4F subunit of the eukaryotic translation initiation factor (eIF4F) complex in infected cells and bound directly to the eIF4E protein. As has been previously demonstrated for feline calicivirus, a member of the <I>Vesivirus</I> genus, PSaV translation required eIF4E and the interaction between eIF4E and eIF4G. Overall, our study provides new insights into the novel mechanism of sapovirus translation, suggesting that sapovirus VPg can hijack the cellular translation initiation mechanism by recruiting the eIF4F complex through a direct eIF4E interaction.</P><P><B>IMPORTANCE</B> Sapoviruses, which are members of the <I>Caliciviridae</I> family, are one of the causative agents of viral gastroenteritis in humans. However, human sapovirus remains noncultivable in cell culture, hampering the ability to characterize the virus infectious cycle. Here, we show that the VPg protein from porcine sapovirus, the only cultivatable sapovirus, is essential for viral translation and functions via a direct interaction with the cellular translation initiation factor eIF4E. This work provides new insights into the novel protein-primed mechanism of calicivirus VPg-dependent translation initiation.</P>

Giftedness from Socio-economically Disadvantaged Families : Alternative Pathways to Talent Development

Rhoda Myra Garces-Bacsal 순천향대학교 특수아동교육연구소 2016 특수·영재교육저널 Vol.3 No.2

“It takes a village to raise a child” is a trite adage that resonates a certain truth particularly to musical talents coming from a developing country. This presentation is an attempt to document an alternative pathway to talent development in music that highlights cultural influences and the power played by stark social realities in lieu of material and intellectual resources required for one to advance in the domain. In Bloom’s (1985) stages of talent development, there is an implicit assumption that the young artist would have the resources for skilled mentors and access to much-needed resources in their pathways to expertise. Through multiple in-depth interviews conducted with two award-winning, highly-respected artist-collaborators from the Philippines, it was evident that there exists an alternative pathway towards eminence that involves exposure to music in the context of daily living and the active role played by the entire community in furthering musical talent. Moreover, one is able to discern how meaning making is achieved through art – with music used as a form of social commentary. Implications of these findings for educators and practitioners are likewise explored.

Porcine sapovirus replication is restricted by the type I interferon response in cell culture

Hosmillo, Myra,Sorgeloos, Fré,dé,ric,Hiraide, Rintaro,Lu, Jia,Goodfellow, Ian,Cho, Kyoung-Oh Society for General Microbiology 2015 The Journal of general virology Vol.96 No.1

<P><I>Porcine sapovirus</I> (PSaV) of the family <I>Caliciviridae</I>, is the only member of the genus <I>Sapovirus</I> with cell culture and reverse genetics systems. When combined with the piglet model, these approaches provide a system to understand the molecular basis of sapovirus pathogenesis. The replication of PSaV in cell culture is, however, restricted, displaying an absolute requirement for bile acids and producing lower levels of infectious virus than other caliciviruses. The effect of bile acids has previously been linked to a reduction in the signal transducer and activator of transcription (STAT1)-mediated signalling pathway. In the current study, we observed that even in the presence of bile acids, PSaV replication in cell culture was restricted by soluble factors produced from infected cells. This effect was at least partially due to secreted IFN because treatment of cells with recombinant porcine IFN-β resulted in significantly reduced viral replication. Moreover, IFN-mediated signalling pathways (IFN, STAT1 and the 2′,5′-oligoadenylate synthetase) were activated during PSaV infection. Characterization of PSaV growth in cell lines deficient in their ability to induce or respond to IFN showed a 100–150-fold increase in infectious virus production, indicating that the primary role of bile acids was not the inactivation of the innate immune response. Furthermore, the use of IFN-deficient cell lines enabled more efficient recovery of PSaV from cDNA constructs. Overall, the highly efficient cell culture and reverse genetics system established here for PSaV highlighted the key role of the innate immune response in the restriction of PSaV infection and should greatly facilitate further molecular studies on sapovirus host–cell interactions.</P>

Giftedness from Socio-economically Disadvantaged Families: Alternative Pathways to Talent Development

Rhoda Myra Garces-Bacsal 순천향대학교 특수아동교육연구소 2016 특수·영재교육저널 Vol.3 No.2

“It takes a village to raise a child” is a trite adage that resonates a certain truth particularly to musical talents coming from a developing country. This presentation is an attempt to document an alternative pathway to talent development in music that highlights cultural influences and the power played by stark social realities in lieu of material and intellectual resources required for one to advance in the domain. In Bloom’s (1985) stages of talent development, there is an implicit assumption that the young artist would have the resources for skilled mentors and access to much-needed resources in their pathways to expertise. Through multiple in-depth interviews conducted with two award-winning, highly-respected artist-collaborators from the Philippines, it was evident that there exists an alternative pathway towards eminence that involves exposure to music in the context of daily living and the active role played by the entire community in furthering musical talent. Moreover, one is able to discern how meaning making is achieved through art - with music used as a form of social commentary. Implications of these findings for educators and practitioners are likewise explored.

It's not only to say to patriarchy

Diarsi, Myra Ewha Womans University Press 1996 Asian Journal of Women's Studies(AJWS) Vol.2 No.-

초록없음

Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus

Kim, Deok-Song,Hosmillo, Myra,Alfajaro, Mia Madel,Kim, Ji-Yun,Park, Jun-Gyu,Son, Kyu-Yeol,Ryu, Eun-Hye,Sorgeloos, Frederic,Kwon, Hyung-Jun,Park, Su-Jin,Lee, Woo Song,Cho, Duck,Kwon, Joseph,Choi, Jong- Public Library of Science 2014 PLoS pathogens Vol.10 No.6

<▼1><P>Sapovirus, a member of the <I>Caliciviridae</I> family, is an important cause of acute gastroenteritis in humans and pigs. Currently, the porcine sapovirus (PSaV) Cowden strain remains the only cultivable member of the <I>Sapovirus</I> genus. While some caliciviruses are known to utilize carbohydrate receptors for entry and infection, a functional receptor for sapovirus is unknown. To characterize the functional receptor of the Cowden strain of PSaV, we undertook a comprehensive series of protein-ligand biochemical assays in mock and PSaV-infected cell culture and/or piglet intestinal tissue sections. PSaV revealed neither hemagglutination activity with red blood cells from any species nor binding activity to synthetic histo-blood group antigens, indicating that PSaV does not use histo-blood group antigens as receptors. Attachment and infection of PSaV were markedly blocked by sialic acid and <I>Vibrio cholerae</I> neuraminidase (NA), suggesting a role for α2,3-linked, α2,6-linked or α2,8-linked sialic acid in virus attachment. However, viral attachment and infection were only partially inhibited by treatment of cells with sialidase S (SS) or <I>Maackia amurensis</I> lectin (MAL), both specific for α2,3-linked sialic acid, or <I>Sambucus nigra</I> lectin (SNL), specific for α2,6-linked sialic acid. These results indicated that PSaV recognizes both α2,3- and α2,6-linked sialic acids for viral attachment and infection. Treatment of cells with proteases or with benzyl 4-O-β-D-galactopyranosyl-β-D-glucopyranoside (benzylGalNAc), which inhibits <I>O</I>-linked glycosylation, also reduced virus binding and infection, whereas inhibition of glycolipd synthesis or <I>N</I>-linked glycosylation had no such effect on virus binding or infection. These data suggest PSaV binds to cellular receptors that consist of α2,3- and α2,6-linked sialic acids on glycoproteins attached via <I>O</I>-linked glycosylation.</P></▼1><▼2><P><B>Author Summary</B></P><P>Although enteropathogenic sapoviruses and noroviruses are leading causes of acute gastroenteritis in both humans and animals, the study of viral pathogenesis and immunity of these ubiquitous pathogens has been hampered due to the lack of a fully permissive cell culture system. Porcine sapovirus Cowden strain provides a suitable system that can be used to identify the molecular mechanisms of viral pathogenesis. Previous studies have shown that carbohydrates and glycolipids play important roles in the attachment of members of the <I>Caliciviridae</I>; histo-blood group antigens (HBGAs) are used by <I>Norovirus</I> genogroups I to IV, as well as members of the <I>Lagovirus</I>, and <I>Recovirus</I> genera, whereas terminal sialic acid is recognized as a receptor for feline calicivirus and murine norovirus. To date, however, the role of carbohydrates in the life cycle of sapoviruses has remained largely unknown. We found that porcine sapovirus binds to susceptible host cells through both α2,3- and α2,6-linked terminal sialic acids which are attached to <I>O</I>-linked glycoproteins. These efforts, findings and insights will significantly contribute to a better understanding of the sapovirus life cycle.</P></▼2>