쑥갓(Chrysanthemum coronarium L.)으로부터 배당체의 분리

송명종,김세영,송효남,백남인 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-

쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. n-BuOH분획으로부터 silica gel 및 ODS column chromatography를 반복하여 배당체를 분리하였다. 화합물의 화학구조는, MNR 및 MS 등의 스펙트럼을 해석하여 1-o-β-D-glucopyranosyl-3-hexenol으로 동정하였다. The aerial parts of Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. n-BuOH fraction gave a glycoside compound through application of silica gel column chromatographies including ODS. The chemical structure of the glycoside was determined by the interpretation of several spectral data, including NMR and MS as O-β-D-glucopyranosyl-3-hexenol (1).

HID 램프 수명 연장을 위한 적응형 점화기 개발

박종연,조계현,송명석 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

The metal halide lamps are now widely used in the application and commercial lighting due to their attracting properties such as good color rendering and high efficiency. But, they have the serious problem of acoustic resonance for high frequency operation and they need the high voltage to ignite. So, they have not been applied to indoors. Over the past few years, a considerable number of studies have been conducted on the electronic ballast together with hot restarting and resonance phenomenon. But, very few attempts have been made with the adaptive ignition method according to the lamp state. In this paper, electronic ballast is proposed for metal halide lamps with an igniter for adaptive ignition. The proposed electronic ballast can generate different ignition voltages according to the arc tube state.

중합효소연쇄 반응법에 의한 인형거대세포바이러스(human cytomegalovirus)의 신속한 검출

김의종,오명돈,박기호,신형식,이환종,김남중,최강원 대한감염학회 1996 감염 Vol.28 No.4

목 적 : 인형거대세포바이러스(HCMV)는 면역 저하 환자에서 폐렴, 망막염, 간염 등 치명적인 감염증의 원인이다. 전통적인 세포 배양법으로 HCMV를 분리하기까지는 1주에서 4주가 걸리므로 이 바이러스에 의한 질환이 의심되는 경우 신속한 진단법을 이용한 진단이 바람직하다. 저자들은 한국에서 분리되는 야생주 HCMV를 검출하는데 중합효소 연쇄 반응법을 이용할 경우의 진단적 유용성을 알기 위하여, 전통적인 세포배양법으로 HCMV가 분리된 검체를 이용하여 중합효소 연쇄 반응법의 특이도와 민감도를 평가하였다. 방 법 : 국내 야생주 HCMV 15주, 그리고 전통적인 바이러스 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 중합효소 연쇄 반응을 실시하였다. 중합효소 연쇄 반응은 Towne주의 immediate early antigen의 유전자에서 유리된 primer MIE와 AD169주의 immediate early antigen의 유전자에서 유리된 primer IE를 이용하였다. 증폭 산물은 겔 전기영동 후 EtBR으로 염색하여 자외선 하에서 관찰하였다. Primer IE에 의한 증폭산물은 또한, DNA blot hybridization 방법으로 확인하였다. 결 과 : 1) 국내 HCMV 야생주 15주를 대상으로 중합효소 연쇄 반응을 한 결과 primer IE를 이용한 경우에는 100%(15/15)에서 177bp의 증폭 산물이 관찰되었고, 이들 증폭 산물은 모두 probe IE와 보합 결합되었다. primer MIE를 이용한 경우에는 93%(14/15)에서 435bp의 증폭 산물이 관찰되었다. 2) 세포 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 primer IE를 이용하여 중합효소 연쇄 반응을 한 결과 direct gel analysis 법으로는 73%(11/15)에서, 보합 결합법으로는 87%(13/15)에서 관찰되었다. Primer MIE를 이용한 경우에는 direct gel analysis법으로 87%(13/15)에서 435bp의 증폭 산물이 검출되었다. 결 론 : Primer IE와 MIE를 이용한 중합효소 연쇄 반응법은 국내 야생주 HCMV를 신속히 검출하는데 유용한 검사법이다. Background : Human cytomegalovirus(HCMV) can cause pneumonitis, hepatitis, retinitis and other serious diseases in the immunocompromised patients. It takes 1 to 4 weeks to diagnose HCMV infection by conventional virus culture. Therefore, when HCMV diseases are suspected, a rapid diagnostic method such as polymerase chain reaction(PCR), antigen assay or shell vial culture is desirable. We evaluate the sensitivity and specificity of a PCR for the rapid detection of HCMV wild strains in Korea. Methods : We used 2 sets of primers ; primer IE and primer MIE derived from the sequence for immediate early gene of AD169 strain and Towne strain, respectively. Fifteen clinical isolates of HCMV, suspended in MRC-5 cells, were amplified by PCR. Fifteen urine specimens which were positive for HCMV by conventional virus culture were also amplified. Amplification products were analyzed by polyacrylamide gel electrophoresis. The products from PCR with primer IE were also identified by DNA blot hybridization. Results : PCR using primer IE gave the PCR products in all of the 15 HCMV wild strains. All of these were hybridized with probe IE. When primer MIE were used, 93%(14/15) of the wild strains showed amplified bands by direct gel analysis. When the urine specimens were amplified by PCR with primer IE, amplified bands were seen in 73%(11/15) by direct gel analysis ; 87%(13/15) by hybridization method. When primer MIE were used, 87%(13/15) of the urine specimens showed the PCR products by direct gel analysis. Conclusion : Polymerase chain reaction with primer IE and MIE may be a specific and sensitive diagnostic method for rapid detection of HCMV wild strains in Korea.

혈액에서 분리된 세균의 항생제감수성 : Vitek System을 이용한 최소억제농도측정 Minimal Inhibitory Concentration Determined by Vitek System

김의종,김선주,신보문,박명희,조한익,김상인 대한화학요법학회 1988 대한화학요법학회지 Vol.6 No.1

Minimal inhibitory concentrations of total 148 strains isolated from blood culture at Seoul National University Hospital were determined by Vitek System. 90% MIC of gram-negative bacteria against amikacin was 2-16㎍/㎖, and those against beta-lactams was > 16㎍/㎖ in general, Except Salmonella spp,. 90% MIC against chloramphenicol and tetracycline was >8㎍/㎖. Range of 90% MIC against vancomycin was ≤.5-4㎍/㎖ in case of garm-positive bacteria, except Enterococcus spp. which showed 90% MIC as >16㎍/㎖. Against ampicillin, 90% MIC of Staphylococcus spp. was >16㎍/㎖, but those of Streptococcus spp. was ≤.25-2㎍/㎖. Streptococcus viridans group showed 90%MIC against penicillin as ≤.03㎍/㎖. Susceptibility of gram-negative bacteria was generally 98% against amikacin, and those of gram-positive bacteria was 80% against vancomycin. 34% of Staphylococcus spp. was resistant of oxacillin. These results may be useful for the primary selection and the dosage determination of antibiotics in the treatment of patients with bacteremia.

中學校 科學科의 實驗實習 敎材의 開1發硏究Ⅰ

郭宗欽,朴炳彬,李元求,宋寅命,崔斗文 공주대학교 사범대학 과학교육연구소 1981 과학교육연구 Vol.13 No.1

The present condition of science education has been investigated b means of questionaires, interviews and referring to literature, and the points at issue gave been extracted on the basis of them, and in consideration of the problems various materials for experiment and practice have been developed to be used for the effective inquiry learning. The present condition and problems of science education in junior high schools are as follows; 1) Science teacher's backgrounds for their teaching profession and majors are good, but they are overburdened and the classes are overcrowded. 2) The textbooks now in use contain too much contents, the levels are high, and the books lack in appropriateness. 3) Especially the contents Presented for experimental activities require too many tasks to be digested in a given time. 4) Experimental facilities or instruments are unsatisfatory in their quantity or quality, and instruments or teaching materials that can be used effectively are far from being sufficient. Futhermore, some experimental instruments are too crude to work successfully. 5) Students' elementary knowledge is not sufficient, and the degrees of their interest or understanding are low. 6) With the teaching methods still conservative and the teachers unable to give satisfactory inquiry leaning. the students are disencouraged. 7) Support of experimental facilities, encouragement of teachers, appropriate school inspection, and administrative or financial aids are desired. In this study, materials for every branch of science have been developed to be available for the effective performance of experiments and practices through inquiry. The materials developed in this study are as follows. 1. The following materials are presented in physics: the relations between the heat quantity of water and the temperature change, and between the heat quantity of the electric heat line and the electric current, for group experiments; the observation of Brown motion, Law of Archimedes, altimeters, comparing the heat capacity of metals, the transmission of radiation, the frictional electricity, the interaction of electric current with magnetic needle, and Fleming's right-hand rule, as the reference materials of demonstration; a program learning materials for diffusion of light with experimental kit utilized in learning the properties of light; a program learning material on the diffraction of light with an experimental kit utilized in learning the diffraction and interference of light. 2. In chenistry, the experimental materials for electrolysis are presented as the teaching-learning material of group experiment. Besides, the errors in the experimental materials and inappropriate expressions in the textbooks now in use are corrected and completed: 3. In biology, problems have been extracted by means of questionaires about the general learning contents and the analisis of Japanese textbooks, and the materials for experiment have been developed. The observation of somatic cell division, that of mold, and that of prothallium are presented as the materials for group experiment. Inquiry learning based on experiment and practice should be settled on the scenes of learning, and the suggestions are made to the effect as follows. 1) The quantity of learning should be reduced, and the levels lowered in the new textbooks. 2) The development and distribution of individual learning materials for the master learning are desirable. 3) The items for group experiment should be reduced to the minimum, and the thorough inquiry learning should be pursued, and the greater part should be replaced by the demonstrative experiments or audio-visual materials. 4) The experimental instruments should be manufactured and distributed on the national scale to ensure their solidity and accuracy. 5) The atmosphere in which inquiry learning is performed by the teacher's creative efforts is greatly needed, To support this conditioning, exhibitions of scientific works should be held continually, science museums operated, the research centers of science education cooperated, new materials for practice developed and studied, and the corporations for research of science education fostered. 6) The measures to lighten the teachers' burden and to encourage them should be contrived.

피칸(Carya illinoensis C. KocH)으로부터 지질대사물질의 분리 및 동정

송명종,한민우,양혜정,이대영,노영덕,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

피칸(Carya illinoensis C. Koch) 열매를 80% MeOH로 추출하고, 추출물을 극성에 따라 n-hexane, CHCl_(3), EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc분획으로부터 silica gel column chromatography를 반복하여 4종의 지질화합물을 분리하였다. 분리된 화합물 화학구조는, NMR 및 MS 등의 스펙트럼을 해석하여 oleic acid methyl ester, linoleic acid methyl ester, β-sitosterol, daucosterol(3-O-β-D-glucopyaranosyl β-sitosterol)으로 동정하였다. Carya illinoensis C. Koch was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, CHCl_(3), EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as oleic aicd methyl ester(1), linoleic aicd methyl ester(2), β-sitosterol(3), and daucosterol(4). They were the first to be isolated from C. illinoensis.

가정과 교육내용에 대한 한국과 일본 여 중고생의 학습관심도에 관한 연구 : 의생활내용 중 의복구성분야를 중심으로 Focused on the Clothes Construction and Making of the Clothing and Textiles Unit

강명희,정영숙 한국 가정과 교육 학회 1990 한국가정과교육학회지 Vol.2 No.-

The purpose of this study is to investigate the degree of learning interest in the curriculum of home economics for the middle and high school girls in korea and Japan, and to obtain the basic guidance for the improvement of the effect of home economics education. In this study, korea and Japanese textbooks were compared and the questionaires were administered to 290 middle school girls and 270 high school girls in Chong-Ju, korea, and 261 middle school girls and 248 high school girls in Tokyo. Japan. The obtained data were analyzed by percentile and x?-test. In comparison of the degree of interest in hand sewing and machine sewing, korean middle school girls showed higher interest than the high school girls, on the other hand, in Japan, the high school girls were more interested. In the unit of making a simple clothes, the middle school girls of both countries were more interested than the high school girls, and the degree of interest of Japanese girls was higher than that of korean girls.

Skyradiometer를 이용한 공주지역의 에어러솔의 광학적 특성

곽종흠,서명석,이태희,곽서연 公州大學校 基礎科學硏究所 2004 自然科學硏究 Vol.11 No.-

본 연구에서는 공주대학교 자연대 옥상에 설치된 분광복사측정기의 일종인 Skyradiometer로 측정된 직달 및 산란 일사량을 이용하여 간접적으로 공주지역의 에어러솔의 광학적 특성을 분석하였다. 분석기간은 2004년 1월부터 11월까지이며 에어러솔의 광학적 특성의 분석에 활용된 알고리즘은 Nakajima et al.(1996)이 개발한 Skyrad.pack.3이다. 주요 분석요소는 에어러솔의 광학두께(AOT), 단일 산란비율(SSA), 옹그스토롬 지수(α) 및 에어러솔의 크기별 분포이며 분석결과의 질을 높이기 위하여 초기 관측 자료의 품질검사를 수행하였다. 공주지역의 11개월 평균 AOT, α 및 SSA는 각가 0.6, 1.1, 그리고 0.9이며 계절 및 기상현상에 따라 큰 시간변동을 보이고 있다. 크기별 입자분포에서는 미세입자와 조대입자에서 최대치가 나타나는 바이 모달 형태를 보이고 있으며 황사(Asian dust) 기간인 봄철에는 조대입자의 총 부피가 크게 증가하였다. 이러한 현상은 봄철동안에 AOT와 SSA가 각각 크게 증가하고 감소하는 점에서도 확인되고 있다. 또한 공주지역의 에어러솔은 풍향에 큰 영향을 받지 않는 것으로 나타나고 있다. We investigate the optical properties of aerosols over Gongju by indirect method using the ground measurement, Skyradiometer. The analysis period is from January to November, 2004. Skyrad.pack.3 is used to estimate the optical properties, such as the aerosol optical thickness(AOT), single scattering albedo(SSA), Angstrom exponent(α) and size distribution, of aerosols from the ground measured radiance data. And quality control process is applied to the raw data to improve the quality of analysis results. The 11-month average of AOT, α and SSA are 0.6, 1.1, and 0.9 respectively. The averaged volume spectra of aerosols show a bi-modal distribution, first peak at fine mode and second peak at coarse mode. Asian dust events clearly increase the AOT and coarse particle, however it decrease the SSA. The effect of wind direction on the optical properties of aerosols over Gonju is not manifest.

가족성 팔목터널 증후군 1가족례

이명종,김광국,이상암,마효일,윤준오 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.1

This is a report of familial carpal tunnel syndrome(CTS) occuring in four members of two generations of a Korean family. One of those with CTS had surgical release and its pathologic finding was nonspecific fibrotic change. The age of those affected by CTS ranged form 35 to 76 years of age. Family pedigree suggests an autosomal dominant gene. This is belived to be the first case report of familial carpal tunnel syndrome in Korea.

지역사회 획득 폐렴의 원인으로서 Chlamydia pneumoniae의 중요성에 관한 연구

김의종,김성민,오명돈,김선주,최강원 대한감염학회 1995 감염 Vol.27 No.1

연구 배경 : Chlamydia pneumoniae(TWAR)는 급성 호흡기 감염증을 일으키는 병원체로, 혈청역학적 연구 결과에 의하면 세계적으로 성인의 20-50%가 C. pneumoniae 대한 IgG 항체를 가지고 있다. 우리나라의 경우, 건강한 성인의 약 50%가 항체 양성임을 고려하면 C. pneumoniae는 우리나라에서도 호흡기 감염증의 흔한 원인중 하나일 것으로 생각된다. 대상 및 방법 : 건강인 108명과 1993년 2월부터 5월 말까지 신병 훈련소에 입대한 군인 중 폐렴으로 인해 후송병원에 입원한 환자를 대상으로 하였다. C. pneumoniae에 대한 IgG 및 IgM 항체는 microimmunofluorescent 법을 이용하여 측정하였다. 항체검사에 사용한 elementary body 항원은 Washington research Foundation으로부터 분양을 받았다. 현증 감염은 항체 역가가 4배 이상 상승하거나, IgG항체 역가 ≥512, 또는 IgM항체 역가>16으로 정의하였다. 성적 : 건강인 108명 중 IgM 항체 양성자는 한 명도 없었으나, IgG >16인 사람은 67명 (62%) 이었다. 폐렴에 걸린 신병 79명의 증상은 기침, 객담이 100%, 발열 87%,수포음 78%, 흉부 X-선상 침윤이 87%였고, 증상 발현 후 입원까지의 기간은 1주일이내가 27%, 8-13일 27%, 14-20일 27%, 21일 이상 20% 였다. 이들 폐렴 환자들 중 C. pneumoniae에 대한 lgG 항체 역가가 4배 이상 상승한 환자는 11명, lgG 항체 역가 ≥512인 환자가 4명 (3명은 항체 역가가 4배 이상 상승)으로, 전체 대상환자 중 15%(12/79)가 급성 간염증의 소견을 보였다. 결론 : C. pneumoniae 감염은 비교적 흔히 발생하며, 지역 사회 획득 폐렴의 중요한 원인 중 하나로 생각된다. Background : Chlamydia pneumoniae strain TWAR cause acute infections of the respiratory tract. Previous study showed that prevalence of TWAR antibody in adults from different areas of the world ranged from 25% to 50%. In Korea, positive rate of poly- IgG antibodies against TWAR was reported to be about 50% among healthy adults. These suggest that infections caused by TWAR may be quite prevalent in Korea. We tried to determine whether TWAR is an important pathogen of community-acquired pneumonia. Methods : We studied 108 healthy persons and 79 military recruits with pncumonia who were evacuated from the training camp to the military hospital from February to April 1993. IgG and IgM antibodies to TWAR were measured using the microimmunofluorescence method. Elementary body antigen from TWAR was provided by the Washington Research Foundation. Serologic evidence of current infection was defined as a four-fold rise in antibody titer. In addition, a titer of 16 or greater in the IgM fraction and of 512 or greater in the IgG fraction were considered presumptive evidence of current or recent infection. Results : Of the 108 healthy subjects, none had IgM TWAR antibody, while 67 (62%) had IgG antibody at a titer of 16 or greater. Of the 79 military recruits with pneumonia, cough and sputum developed in 100%, fever in 87%, and crackles in 78% Chest X-ray infiltration was noticed in 87%. Duration of symptoms before admission was less than 7days in 27%, 8-13 days in 27%, 14-20 days in 27%, 21 days or longer in 20%. Of the military recruits with pneumonia, 15% (12/79) showed evidence of acute TWAR infection: 11 showed a four-fold antibody titer rise, and 1 had IgG antibody titer greater than 512. Conclusion : These results suggest that TWAR infections are quite prevalent and TWAR may be one of the important pathogens of community-acquired pneumonia in Korea.