枸杞子의 原形質體分離와 培養에 關한 硏究

朱明七 동신대학교 환경연구소 2003 환경연구 Vol.8 No.1

枸杞子의 重要用途는 觀賞樹, 庭剛樹 等 造景用 素材外에 藥用植物로 使用된다. 구기자의 體細胞雜種 合成을 爲한 生物工學的 基礎資料를 確立하기 爲하여 原形質體分離 및 培養實驗을 實施하여 實生苗의 葉肉組織으로부터의 원형질체관출은 Macerozyme 3%와 Cellulase 1% 組合, 0.4M Mannitol, pH 6.2, 6時間의 處理에서 또한 材料의 葉齡이 낮을수록 原形質體 數量과 生存率이 높음이, MSP_(19)M 培地(NAA 2.0mg/ℓ+BAP 0.5mg/ℓ)에 原形質體 密度別로 培養한 結果 5x10⁴mℓ에서 18日째 budding이 일어났으며 26日後에 cell colony의 形成을 觀察할 수 있었다. This study was carried out in order to develop techniques for rapid propagation and afford the basic imformation for somatic hybrid of Lycium chinense, used landscape woody plant and chinese herbal medicines, through protoplast isolation and culture were examined. Results obtained were summarized as follows. 1. The highest protoplast yield and viability were in 3.0% macerozyme and 1% cellulase R-10, 0.4M mannitol, pH6.2 and incubation for 6hours.And the more effective at the more juvenile leaf tissue. 2. When protoplasts were cultured at 5x 10 /ml density in MSP19M medium, the first budding occured at 18 days after culture, 28 days later Cell colony was formed.

다중이용업소의 내장재 규정의 적용에 관한 연구

이주헌,윤명오,김운형 한국화재소방학회 2001 한국화재소방학회논문지 Vol.15 No.1

건물 내장재는 화재 발생 시 화염 확산 및 유동성 가스발생의 주원인으로 피난안전계획에 매우 중요하지만 다중이용업소의 인테리어 공사 시 화재성능에 대한 검증 없이 무분별하게 사용되고 있어 동일한 유형의 인명피해가 반복되고 있다. 따라서 본 연구에서는 국내 내장재 사용 실태조사를 통해 현행 관련규정상 문제점을 살펴보고, 이를 효과적으로 개선, 보완할 수 있는 방안을 제시하였다. 연구 결과, 구조체 밀착재를 제외한 내장재는 가칭 화재안전코드규정에서 사용구제하고, 소방 · 방화완비중명제도 시행 전 허가 · 신고된 다중이용업소는 건축법시행령에 소급하여 적용 할 수 있는 근거를 마련하며 지하 소규모 업소에 대한 소방방화시설 법제화, 내부 용도 변경 시 내장재사용 도면첨부 및 무단 변경 시 관련기관에 통보조치, 이동성가구에 대한 방염 처리 의무조항 신설, 화재확산 및 연기 유독성을 기준으로 하는 재료등급 평가 및 시험방법의 개선이 필요한 것으로 사료된다. A Interior material, a main cause of fire-growth and generating toxic gas it burns, should be dealt with great care in life safety design. Nonetheless, it has been used recklessly with undue attention to its contribution to fire in particular in entertainment occupancy and causes many victims in fire, Therefore, this study attempts to examine the current use of interior material in Korea and find out what to be improved and enhanced in terms of related regulations. Based on the comparison and analysis of the Korea regulation with those of advanced nation, suggestions are made for an effective and efficient improvement and complement to the current system. What can be suggested from this study are as follows. · The use of interior material should be controlled under the unified regulation of fire-safety codes. · Code should be set up so that the current construction enforcement should be applied in retroactive to those entertainment buildings that obtained a license prior to the implementation of the system certifying that the building is fire-resistant and fire-protection. ·The legislation should be made to control the fire-protection facilities of small-sized, underground entertainments. · It should be obliged to present the blueprint displaying the use of interior material at the time of changing occupancy. Or, it should be compelled to report changes that go way without permit to the administrative office. · A compulsory provision should be set up to have a fire-resistant performance to movable furniture. · The classification index designating the fire hazard of interior material by flame spread rate and smoke toxicity and its test method should be established.

지하공동구 화재 실험 및 시뮬레이션에 의한 화재 실험 예측 평가

윤명오,고재선,박형주,박성은 한국화재소방학회 2001 한국화재소방학회논문지 Vol.15 No.1

지하공동구는 최근 통신의 발달로 인해 국가 중추기능으로서의 주요 역할를 하는 시설로, 사고시 신속한 대처가 힘들고 케이블 연소시 발생하는 유독가스에 의한 검은 연기로 공동구 내에 진입하여 소화하기가 힘들다. 따라서 화재발생시 막대한 재산피해 및 통신의 두절 등 국가의 중추신경이 마비됨은 물론 시민 불편사항을 초래시켰다. 본 논문은 지금까지 발생되어온 공동구 화재를 바탕으로 실제 공동구 모형을 제작하여 화재를 재현함으로서, 과학적으로 화재의 성상을 분석하고 지하공동구 내에 소방설비를 갖춘 후에 일정온도에서 각각의 소방설비들이 제대로 작동하는지를 검증하는데 그 목적이 있다. 화재실험은 지하공동구 내에 정온식 감지선형 감지기, 방화문, 연결살수설비 및 환기설비를 설치하고 송 · 배전케이블은 일정구간 내화도료로 도장하며 난방관은 내화피복된 상태에서 실험하였다. 그 결과 최고 온도가 932℃로 측정되었고 일정온도에서 정온식 감지선형 감지기가 작동하여 화재위치를 정확히 수신반에 나타내었다. 그리고 송 · 배선케이블은 일정구간 내화도료로 도장한 것은 내화성능이 없는 것으로 나타났고, 내화피복된 난방관은 약 30분 정도의 내화 성능이 있는 것으로 나타났다. 컴퓨터 화재 시뮬레이션은 실제 화재 시험시의화재하중을 입력하여 실시한 결과 최고 온도가 943℃로 실제 화재시의 최고 온도인 932℃와 거의 일치하였다. 따라서 공동구 화재하중만으로 화재 시뮬레이션을 실시하여 화재의 성상에 대한 예측이 가능한 것으로 판단되며 시뮬레이션으로 얻는 열방출량, 연기층의 높이, 산소, 일산화탄소, 이산화탄소의 농도 등의 결과 값들을 실제 화재 실험시의 값으로 적용시킬 수 있는 것으로 판단된다. The utility tunnels are the important facility as a mainstay of country because of the latest communication developments. However, the utilities tunnel is difficult to deal with in case of a fire accident. When a cable burns, the black smoke containing poisonous gas will be produced. This black smoke goes into the tunnel, and makes it difficult to extinguish the fire, Therefore, when there was fire in the utility tunnel, the central nerves of the country had been paralyzed, such as property damage, communication interruption, in addition to inconvenience for people. This paper is based on the fire occurred in the utilities tunnel in the past, and reenacting the fire by making the real utilities tunnel model. The aim of this paper is the scientific analysis of the character image of the fire, and the verification of each fire protection system whether it works well after process of setting up a fire protection system in the utilities tunnel at a constant temperature. The fire experiment was equipped with the linear heat detector, the linear heat detector, the fire door, the connection water spray system and the ventilation system in the utilities tunnel. Fixed portion of an electric power supply cable was coated with a fire retardant coating, and a heating tube was covered with a fireproof. The result showed that the highest temperature was 932℃ and the liner heat detector was working at the constant temperature, and it pointed at the place of the fire on the receiving board, and Fixed portion of the electric power supply cable coated with the fire retardant coating did not work as the fireproof. The heating tube was covered with the fireproof had the function of the fireproof about 30 minutes.

竊盜小女의 MMPI에 關한 硏究

盧明來,朱根 순천향대학교 1997 Journal of Soonchunhyang Medical Science Vol.3 No.3

기계연마에 기인한 Pd(100)의 표면 지형에 대한 AFM 분석

박현주,정경한,추명석,진원배 동아대학교 기초과학연구소 2001 基礎科學硏究論文集 Vol.18 No.1

기계 연마에 기인하는 Pd(100) 표면의 지형 및 연마과정 중에 행해지는 표면 세척방법에 따라 표면 거칠기에 미치는 영향을 AFM으로 조사하였다. 연마 입자의 크기별로 순차적으로 3, 1, 0.1, 0.04 ㎛의 입자로 연마를 수행하였다. 연마의 매 단계마다 시료 표면에 남아있는 연마제를 제거하기 위하여 시료 표면을 단순히 흐르는 물로 세척하는 경우와, 흐르는 물에서 솜으로 세척하는 경우의 표면 거칠기를 비교한 결과, 제곱평균 제곱근에 의한 표면 거칠기는 5×5 ㎛2의 주사영역에서 평균적으로 각각 1.61, 1.08, 0.82 nm 정도의 차이로, 후자의 경우가 훨씬 효과적임을 보여주었다. 이러한 사실로부터 단순히 흐르는 물에 의한 세척으로는 연마의 전 단계에서 사용된 연마 입자들이 표면상에서 완전히 제거되지 않고 다음 단계의 연마 결과에 영향을 미친다는 것을 알 수 있었다. 또한 연마과정 중 bulk로부터 빠져 나온 것으로 보이는 알갱이들이 연마입자에 의해서 발생하는 스크래치의 가장자리를 따라 흡착되어 있는 것이 관측되었다. The effect of surface cleaning performed at every step of mechanical polishing process on surface roughness was investigated with AFM surface topography. Diamond suspensions with grain sizes of 3, 1, 0.1 ㎛ and colloidal silica of 0.04 ㎛ for final polishing were used as abrasive in turn. When the specimen was cleaned with only flowing water at each polishing step, some part of particles remained on the specimen after cleaning. These remaining particles gave rise to relatively pronounced scratches on the surface during the subsequent polishing step, In the other hand, in the case cleaned with a soft wool in flowing water, it was showed that the remaining particles were removed almost all. The lower degree of rms-surface roughness in the latter case compared to that of the former case was obtained, as the differences of rms-surface roughness between both cases were on an average 1.6 nm,1.1 nm, and 0.8nm, respectively, for an area of 5×5 ㎛2 after polishing with 1, 0.1, and 0.04㎛ grain size. In addition, it was found that particles are deposited at the edges of the scratches, which are probably Pd particles removed by the mechanical polishing.

로마 가족 내에서의 부모-자녀 관계 : 공화정 말, 제정 초를 中心으로 From the Late Republic to the Early Empire

申明珠 한국서양고대역사문화학회 ( 구 한국서양고대사학회 ) 1999 서양고대사연구 Vol.7 No.-

The purpose of this study is to examine the features of the Roman family, especially parents-children relationships in Roman society from the second century BC to the second century AD. It has been thought that the Roman family was the extended family consist of several conjugal families and all members in this extended family were unconditionally subject to the authority of the oldest male of the family. This image was due to two facts. It was one thing that the words familia and domus which meant "family" in Roman society imply the extended family. It was the other that the authority of man who is sui iuris (patria potestas) affected all his descendants including children, grandchildren and so on during his life time. However, recent studies in the Roman sepulchral inscriptions and the ruins of Roman houses revealed that many of Roman families were nuclear families consist of one couple and their children. Romans thought the family as not only the reproduction unit but also the bond of affection and "haven in a heartless word" High infant mortality and frequent child exposure were the realities of Roman society, but it does not mean that Roman parents were not attached to their children. Roman fathers were thought as arbitrary and despotic rulers of their children because of the character of patria potestas. Patria potestas meant the full control of his descendants including the power of life and death(vitae necisque potestas) and the monopoly of property. This legal authoritarianism was the reality of the Roman family. However, it does not seem that patria potestas exercised in full extent like the power of life and death and there existed only authority and obedience between Roman fathers and sons. Romans knew that the basics of the family are not only authority but also affection and devotion. We can find the familial affection and devotion in the notion of pietas. Fathers-sons relationships in Roman society implied reciprocal responsibility, affection and discord as in another society. Roman fathers took the direct role in the education of their sons or had a strong sense of responsibility about it. They chose the golden mean between severity and indulgence to direct their sons. Romans favored sons to succeed the family's name, but Roman daughters had equal rights of their fathers' property with their brothers in the law. We can find many examples of the fathers' care and affection for their daughter in Roman materials. In the eye of the law, Roman mothers had not the same authority as that of Roman fathers. However, we can't judge mothers-children relations only by the law. It was socially granted that mothers were respected by their children and took part in the upbringing of them. The inheritance between mothers and children had appeared in earlier times, but had legal force in the second century AD. Nurses appeared frequently in the Roman high class, but it was the ideal for Romans that the mother took care of her children with her own hands. The image of the widowed mother who was responsible for the education of her sons was the ideal in Roman society. It was concerned with the fact that Roman mothers could live longer with their children than Roman fathers owing to the difference of the age of marriage. Roman mothers often exercised strong influence on adolescent sons. The bonds between mothers and daughters might have been strong, though they didn't reveal clearly because all materials were written by males. Parents-children relationships in Roman society were not unilateral but reciprocal. The strong authority of fathers was the reality in the Roman family, but we can see the affectionate bonds between Roman parents and children. It reveals the fact that the family in past times was understood with complexity, not simplicity.

폴리머 콘크리트 휨부재의 휨 및 전단거동 특성

연규석,김남길,주명기 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

본 연구에서는 압축강도 1,400 kgf/㎠를 갖는 불포화 폴리에스터 폴리머 콘크리트를 이용하여 제작한 C형 시험체에 대한 편심압축 시험을 통하여 폴리머 콘크리트 휨부재의 응력-변형률 관계를 구명하고 등가직사각형 응력블럭의 파라미터 α,β_1, ν를 산출하였다. 아울러, 인장철근비를 0.038, 0.076 및 0.094로 하고 전단스팬비(a/d)를 1.5, 2.5 및 3.5로하는 9종의 철근 보강 폴리머 콘크리트 보를 제작하고 이에 대한 전단거동을 고찰하였다. 실험결과 등가직사각형 응력블럭의 파라미터 α,β_1, ν는 각각 0.61, 0.73, 0.845로 나타났으며, 사인장강도 및 극한전단강도의 예측식인 ACI 규준식 및 Zsutty 제안식을 수정하여 고강도 폴리머 콘크리트의 전단강도예측식을 제안할 수 있었다. 그러나, 보다 정확한 전단설계를 위해서는 스트럿-타이 모델이나 트러스 모델을 적용한 이론 및 실험적인 연구가 더 이루어져야 할 것이다. In this study, C-shaped specimens have been made with the unsaturated polyester resin of which the compressive strengths is 1,400 kgf/㎠. The stress-strain relations of polymer concrete beam member was examined by using the eccentric compressive test and the output stress block parameters α, β_1, γ were obtained by using the equivalent rectangular stress block. Three different tensile reinforced ratios, 0.038, 0.076, 0.094, and shear span ratios (a/d) 1.5, 2.5, 3.5 were used to make nine kinds of reinforced polymer concretes and the shear behaviors were investigated. The experimental results showed that the parameters, α, β_1, γ, of equivalent rectangular stress block were obtained as 0.61, 0.73, 0.84, respectively. The prediction formula of diagonal strength or the limit shear strength shown in the ACI standard and the Zsutty proposal was modified and applied for the proposal shear strength prediction formula of high-reinforced polymer concrete. In order to do the precise shear design, however, theoretical and experimental researches are thought to be continued by applying the strut-tie model or the truss model.

소나무와 리기다소나무의 수간류 및 수관통과우의 화학적 성질

이상덕,이명종,주영특 강원대학교 삼림과학연구소 2001 Journal of Forest Science Vol.17 No.-

수간류, 수관통과우의 화학적 성질변화에 미치는 수종간 차이의 영향을 비교하기 위하여 강원대학교 구내의 소나무림과 리기다소나무림을 대상으로 하여 조사, 분석하였다. 임의우의 평균 pH는 6.2였으며, 소나무의 수관통과우>소나무의 수간류>리기다소나무의 수관통과우>리기다소나무의 수간류 순의 평균 pH값을 보였다. 또한 6월을 제외한 대부분의 조사기간 동안 임의우의 pH가 수간류와 수관통과우의 pH보다 높게 나타났다. 다른 원소에 비하여 양이온은 Ca2+과 K+이, 음이온은 NO3-과 SO42-이 타원소에 비하여 높게 나타났으며, 특히 11월에 K+, Mg2+, Na+이 증가하였다. 한편, 소나무와 리기다소나무의 수간류와 수관통과우에 있어서의 용존원소 농도는 임외우 보다 높게 나타났다. This research was carried out to investigate the effects of the different tree species (Pinus densiflora and Pinus rigida) about the chemical properties of stemflow and throughfall at Chuncheon city in Kangwon province. The water quality of the rainfall, stemflow and throughfall in each forest stands were analyzed chemically. The pH average of precipitation was pH 6.2 and pH averages were throughfall of P. densiflora> throughfall of P. rigida> stemflow of P. rigida during the study period. During the all period, the pH of precipitation was higher than the pH of stemflow and throughfall except on June. The concentration of cation(Ca2+ and K+) and anion(NO3- and SO42-) were higher than others. Especially on November, the concentration of K+, Mg2+, Na+ were increased. The concentration of dissolved elements of stemflow and throughfall (Pinus densiflora and Pinus rigida) were higher than precipitation. Key words : precipitation, stemflow, throughful, Pinus densiflora, Pinus rigida,

라텍스 개질 콘크리트의 강도발현 및 내구특성

윤경구,홍창우,이주형,이종명 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

본 연구는 라텍스 혼입률이 5%, 10%, 15%, 20%로 변화할 때 라텍스 개질 콘크리트의 내구 특성을 평가하는데 있다. 라텍스를 콘크리트에 혼입한 라텍스 개질 콘크리트는 보통 콘크리트의 색상과 성형성, 작업성를 갖으며, 약 20%~35%의 단위수향이 감소된다. 그리고 양생후, 콘크리트는 시멘트수화물과 라텍스 입자의 필름막에 의해 연결된 골재로 구성된다. 일반적으로 라텍스 양이 증가하면 콘크리트의 휨강도는 증가하는데, 이것은 라텍스가 시멘트 수화물과 골재사이의 점착력을 증가시키기 때문으로 판단된다. 라텍스 개질 콘크리트와 보통콘크리트와의 상대투수성을 측정하기 위하여 염소이온투과시험법을 사용하였다. 그 결과 라텍스 개질 콘크리트의 투수성은 보통콘크리트의 투수성보다 매우 낮음을 알 수 있었다. 이것은 라텍스가 미세공극을 충진하고 라텍스 입자의 필름막에 의해 골재와 시멘트수화물이 연결 때문이다. 또한 라텍스가 공극주위로 필름막을 형성하기 때문에 공기량이 투수성에 영향을 미치지 못한다는 것을 알 수 있었다. 보통콘크리트에서는 동결융해저항성을 증가시키기 위해 공기연행제를 필요로 하지만, 라텍스 개질 콘크리트에서는 적절한 양생이 이루어지면 추가적인 공기연행제는 요구되지 않는다. 또한 라텍스 혼입률이 증가함에 따라 추가적인 동결융해저항성의 증가는 나타나지 않았다. This study focused on the investigation of strength development and durability of latex modified concrete(LMC) as the latex content variated such as 5%, 10%, 15%, and 20%. When latex was added to concrete, the workability of LMC was quietly increased, compared to that of conventional concrete. This resulted in the decrease of the required water as 20% to 35% in order to obtain a same slump. In general, increasing the amount of latex decreased compressive strength a little but increased flexural strength quite a lot. This might be due to interconnection by a film of latex particles between hydrated cement and aggregates. This, also, might attribute to bonding between the hydrated cement and aggregates. The rapid chloride permeability test was used to evaluate the relative permeability of latex-modified concretes and conventional concretes. The results showed that the permeability of latex-modified concretes was considerably lower than that of conventional concretes tested, which might be due to the latex filled in voids and interconnections of hydrated cement and aggregates by a film of latex particles. Because of latex formed film surrounding the air void, air contents and voids does not effect to the permeability of latex modified concrete.

석탄폐석을 이용한 폴리머 콘크리트의 물리 역학적 특성

연규석,최동순,장태연,주명기 강원대학교 석재복합신소재제품연구센터 석재연 논문집 Vol.1 No.-

최근의 급속한 경제성장과 건설물량의 증가로 인한 수요가 급격히 증대되고 있으나, 양질의 콘크리트용 골재 부족현상을 초래하고 있어 골재의 양적인 문제와 함께 질적인 문제의 해결이 건설재료 분야의 중요한 당면과제로 제기되고 있다. 따라서 본 연구에서는 강원도 탄광지역에 다량으로 산재되어 있는 석탄폐석을 콘크리트용 골재로서 활용하여 고강도이며 내구성이 우수한 폴리머 콘크리트를 제조하고 이에 대한 물리·역학적 특성을 실험적으로 구명하여 공장제품 재료로서의 이용을 위한 기초자료로서 제공코저 한다. Lately, rapid expansion of construction industry and following increment of demand for concrete in the construction created shortage of aggregates in the nation. Supplement of god quality aggregate is an immediate issue for the construction industry to solve. Therefore, this study evaluated a possibility of using coal mine waste collected from Kwangwon-do region as a source of aggregate in manufacturing polymer concretes which have high strength and high durability. First, aggregates were obtained by crushing coal mine waste and polymer concrete was manufactured using these aggregate. Mechanical property test results for the polymer concrete showed that the coal mine waste aggregates were acceptable to use as a replacement of the aggregate in polymer concrete manufacture.