흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

이온화에너지 처리 아이스크림 및 우유의 단회투여 독성시험

이희준,최현종,한선경,구본철,신명수,함준상,조철훈,이완규 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

16S와 23S rRNA에 결합하는 Probe를 이용한 겨울철 소양호 세균 군집 구조의 분석

홍선희,변명섭,안태석 한국동물학회 1995 동물학회지 Vol.38 No.4

수소에 의한 황산구리의 환원속도

이명철,신창섭,이태희 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

Cupric sulfate powder of average diameter 2㎛ was reacted with hydrogen at the temperature range of 240-350℃ in a fixed bed reactor and concentration of effluent sulfur dioxide was measured. Experimental results showed that sulfur dioxide regeneration efficiency of 93.6% was maximum at 300℃. The reaction of cupric sulfate with hydrogen was able to be interpreted by the shrinking unreacted core model with chemical reaction as rate controlling step. The reaction was found to be first order for the hydrogen concentration and the reaction rate constant was k=0.0165 exp (-2047/RT).

Polystyrene 입자 수용액에서 프랙탈 차원에 관한 광산란 실험 연구

강명화,김두철,강창희,현동걸 제주대학교 1994 논문집 Vol.39 No.-

Using light scattering technique, we measured intensities of scattered light from aggregation of polystyrene particles of 114nm in a solution of NaCl in the concentration from 0.15 to 2mol/ℓIntensity data give direct information that aggregated cluster are built with fractal structure ; We find that the value for fractal dimension of aggregation in the fast regime 1.25≤c≤2mol/ℓis 1.55 ±0.05 that is nearly consistent with diffusion-limited-aggregation model, whereas in the slow regime (0.3≤c≤0.5mol/ℓ)the value of fractal dimension is 2.12±0.05 agreed with reaction-limited-aggregation model.

16S와 23S rRNA에 결합하는 probe를 이용한 겨울철 소양호 세균 군집 구조의 분석

홍선희,변명섭,안태석 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

겨울철 소양호에서 세균 군집 구조를 파악하고자 총세균수와 EUB338, ALF1b, BET4a, GAM42a와 CF probe등 fluorescent rRNA targeted oligonucleotide probe와 반응하는 세균 개체수를 수심별로 측정하였다. 총세균수는 0.7×10^(6)~1.1×10^(6)cell·㎖^(-1)이였으며, 5m와 10m 수층에서 높게 나타났다. 총세균수에 대한, Eubacteria의 비율은 34~90%이였으며, 5m와 10m에서 낮게 나타났다. Proteobacteria α-group은 Eubacteria의 10.8-28.7%, β-group은 4.5-53.5%, γ-group은 4.9-35.5%, 그리고 Cytophaga-Flavobacterium group은 6.1-21.1%이였다. 0.5m 수심에서는 β-group이 28.6-53.3%로 우점하고 있었으며, 10m에서는 γ-group이 35.5%로 우점하였다. 30, 50m 수심에서는 α-group과 Cytophaga-Flavobacterium group이 우점하였다. 세균 군집 구조로 보면 0-2m, 5-10m 그리고 30-50 m의 3개층은 각각 독특한 특징을 나타내었다. 이 방법으로 호수 생태계에 대한 새로운 정보를 얻을 수 있었다. To scrutinize the bacterial community composition of Lake Soyang in winter, bacterial numbers blonging to Eubacteria, Proteobacteria and Cytophaga-Flavobacterium group were estimated by using 16S and 23S rRNA targeted oligonucleotide probes. Total bacterial numbers ranged from 0.7Χ10^(6)~1.1Χ10^(6)cell ·㎖^(-1)and vertical profile of total bacteria showed a peak at 5m and 10m depths those were low exhibiting, 39 and 34%, respectively, The percentage of proteobacteria α-group ranged 10.8~28.7%, β-group 4.5~535%, Υ-group 4.9~35.5% and Cytophaga-Flavobacterum group 6.1~21.1%. The dominant groups were β-group at 0, 2 and 5m Υ-group at 10m, α-group at 30m and Cytophaga-Flavobacterium group at 50m depth. In winter season, Lake Soyang can be divided into into three layer, 0~2m, 5~20m and 30~50m, by bacteria community composition. By this method, new informations about aquatic ecostem were developed.

특수기능성섬유가 성장기 운동선수의 수면 중 성장호르몬 분비에 미치는 영향

이명수,이진희,고경찬,문성록 한국의류산업학회 2003 한국의류산업학회지 Vol.5 No.1

The purpose of this study was to investigate the effect of multi-functional fabric on EEG and growth hormone (GH) during sleep and quality of sleep with the 9 young female athletes. The subjects participated in separated experimental procedure; sleeping in multi-functional fabric wear (experimental group) and cotton wear (control group) for 450min. During the night (22:00-05:30), we recorded the changes of nocturnal polysomnographic sleep recording and GH were measured every 60min. The results show that there are significant differences in percentage of stage 1, 2 and slow wave sleep (SWS) between two groups(S1, p<.05;S2, SWS, p<.01). The SWS percentage of experimental group is 1.89 times higher than control group. The changes of GH secretion varied depending on two experimental procedures. The peak of GH secretion in experimental group is more than controls by 2.4time (p<.001). The quality of sleep in experimetal group is significantly higher than control (p<.01). These results suggest muti-functional fabric wear is effective in inducing the deep sleep and increasing GH and quality of sleep.

Tourism English Teaching Techniques Converged from Two Different Angles

성명희 범태평양응용언어학회 2001 범태평양응용언어학회지 Vol.5 No.1

This paper aims to provide some techniques converged from two different angles(learners and tourism English features)for effective tourism English teaching in junior college in Korea. For this, comprehensive methods were undertaken: a questionnaire for learners` needs analysis, an instrument designed by Xiao-hua and Naerssen (1987) for learners` strategies for oral communication, a small-scale classroom study for learners`preference of teaching techniques; interviews for the feature of tourism English teaching. It is suggested that the specific knowledge of the content and the basic expressions of the content be first identified, and then, interaction, an essential factor, be focised on provoking learners` stategies for communication, and be placed on a continuum from predictable interaction to language parctice, along with various groupings, supports of grammar knowledge when necessary.

산발성 장형 위선암 환자의 Microsatellite Instability와 병리학적 양상

조창희,홍유찬,안지현,최경현,이상호,신영명,윤기영,정민정,장희경 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Background : Through many researches, microsatellite is expected to be a good diagnositic and prognostic factor in colorectal cancer, endometrial cancer, gastric cancer, and the others. The prevalence of microsatellite instability (MSI) in gastric carcinoma has reported variously, 13~44%. Purpose : We aimed to determine the prevalence of MSI-high and the relationship between MSI and pathological characteristics of sporadic intestinal type adenocarcinoma of stomach. Material and Methods : We analyzed 106 sporadic intestinal type adenocarcinoma specimens excised from patients who were over thirty-five years old to determine the statue of microsatellite by DNA sequencing. The tissues were formalin-fixed and paraffin embedded. DNA were extracted and amplified by polymerase chain reaction (PCR). MSI was determined using five markers recommended by National Cancer Institute (NCI). Specimens were also studied with five patholical factors-differenciation of tumor cells, depth of invasion, lymph node metastasis, vessel invasion, and perineural invasion- to determine pathological state. Result : The microsatellite statue was determined as MSI-High in 5 cases (4.7%), no MSI-low, and MSS (microsatellite stable) in 101 cases (95.3%). Within the frequency, there was no large gap in the distinction of gender in MSI cases, but in MSS cases, there was three-times more cases in male. MSI cases had moderate-to-poor differenciation and trend to invade toward serosa. All MSI cases showed no perineural invasion. But we could not find any statistical significance between MSI and pathological characteristics of sporadic intestinal type adenocarcinoma. Conclusion : Results suggest that MSI can not make any certain pathological significance in sporadic intestinal type adenocarcinoma. Even though less than 5% of sporadic intestinal type adenocarcinoma patients showed MSI, it can be used as a influential prediagnostic factor of gastric cancer. Further study with large scale of cases will be followed to verify these results.

전자상거래를 통한 DVD 쇼핑몰

김명진,안경희 인제대학교기초과학연구소 2001 자연과학 Vol.5 No.-

인터넷을 이용한 전자상거래를 통한 DVD(Digital Video Disk)쇼핑몰로서, DVD는 전 세계적으로 계속적인 성장을 보일 것으로 예상되며, DVD의 지속적인 개발에 따른 서비스는 전자상거래를 통한 전문 DVD쇼핑몰 증가와 고객들의 소비가 폭팔적으로 증가할 것이다. 전문적인DVD쇼핑몰은 인터넷을 통하여 가정이나 사무실에서 편안하게 영화에 관한 정보 검색 및 관람과 DVD판매, 대여 등을 고객들에게 서비스를 제공함과 동시에, 보다나은 고화질, 고음질의 영상매체를 전달 및 실시간으로 서비스를 제공하여 자료를 전송한다. 본 논문에서는 인터넷을 통한 전자상거래 연구와 이러한 서비스를 바탕으로 제공하는 쇼핑몰 시스템을 설계하여 실제적으로 사용할 수 있게 구현하였다. Electronic commerce is possibly the most promising information technology application that enterprises have seen in recent uears. Internet technology offers five to ten times improvement in reaching new customers, increasing the speed at which business is conducted. The DVD shopping malls are needed for our daily life. This paper is meant to show the design and implementation of a DVD shopping mall for internet users. The first part of our paper is that introduces the design of the mall and its databases, and then processes information between web and internet data.