Aspergillus awamori의 효율적인 protoplast 생성을 위한 homogenization효과

최명락 麗水水産大學校 産業技術硏究所 1995 産業基術硏究所 論文集 Vol.4 No.-

The effect of homogenization for efficient protoplasts formation from Aspergillus awamori was studied. After homogenization for 5 min, a high yield of protoplasts was obtained. Prolonged treatment beyond 15 min decreased the yield of protoplasts. Protoplasts prepared from enzyme treatment after homogenization showed an increase in protoplast yields as compared with the protoplast yields prepared from the only lytic enzyme treatment.The present study shows that high yields of protoplasts can be prepared from Asp. awamori using homogenization before enzyme treatment.

BALLET BARRE 동작시 하지근전도 파형에 관한 연구

홍명엽,박태열 韓國舞踊敎育學會 2000 韓國舞踊敎育學會誌 Vol.11 No.2

In Thib study, studied on Barre exorcise, which was used for developing the primary active muscles. For this study, observed 6 muscles (Vastus Medialis. Gluteus Maximum, Rectus Femoris, Gastrocenemins, Vastus lateralis, Biceps Femoris) far doing the 4 basic motions. The results are as follow. 1.At the Battement - Tendu motion, Front, Side and Back muscled were rosed and the primary active muscle was Gastrocnemins. Ath the muscle, which used as an axle, it baa Rictus Femoris at the Front and Baek. and it wag Gastrocenemins at the Side. There were differences between the Front and Side muscles in using as primary active muscle. There were no differences between them using as an axle. 2.At the Battemenu - Tendu jet□(degage) motion, the primary active muscles were Gastrocnemins when used Fnnt, Side and Back muscled and it was Vastus Lateralis when used Rmnt muscle as an axle. There were differences at the used Front and Side and Were no differences at the Back. when that are used as primary active muscles. There were differences at the Back, which used as an axle, and there were no differences at the used Hront and Side. 3.At the Rond de Jembe a teiTe Motion, the primaiy active muscles were Gastrocnemins when they used Hmnt and Back muscles and it was Rectus Femoris when they used as an axle. There were no differences between them. 4.At the Frapp□s Motion, the primary active muscles were Gastrocnemeins when they used Hmnt, Side and Back, and it wart Rictus Femoris when they used as an axle. There were differences between the used Front and the axle's Back. But there were no differences at the used Side and Back and axle's Front and Side. Finally, Gastrocenemins, Re77us Femoris and Biceps Femoris are used frequently in Ballet. Therefore, these muscle's training will be to develop the lower limb's muscles and to prevent the bodily harm of the dancers.

둔부의 측면 유형과 후면 유형과의 대응 관계 : 만 18~24세 여자대학생을 중심으로 Females from 18 to 24 Years Old

문명욱 東義大學校 生活科學硏究所 2003 生活科學論集 Vol.7 No.-

This research was done to analyze correspondence these side view types with back view types. The paper will offer basic data for clothing design of lower body which is intended to apply appropriate shape and capacity to clothing. In the precedent study, 191 women were classified into three types of side view and four types of back view of lower body from waistline to gluteal furrow line. Correspondence side view types with back view types of lower body from waistline to gluteal furrow line Type 1 of side view(thick and droopy hip) is corresponded with Type 1(wide breadth and straight line) and Type 3(wide breadth and curve line) of back view. Type 2 of side view(slim and the flat hip) is corresponded with Type 2 of back view (narrow breadth and curve line). Type 3 of side view(normal curve) is corresponded with Type 4 of back view(trapezoid shape).

윈드서핑 레저스포츠의 만족도 분석

양명환 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.1

본 연구의 목적은 윈드서핑에 참여하는 동호인들을 대상으로 윈드서핑 참여수준, 성, 연령, 기량수준에 따라서 만족도 지각정도가 어떻게 다른지에 대한 기술적인 분석을 하고, 윈드서핑 활동 만족을 예측할 수 있는 요인들을 규명하고자 하였다. 본 연구의 대상자는 제주도윈드서핑연합회 소속이거나 개별적으로 윈드서핑활동에 참여하는 동호인, 2001년도 제주대학교 하계 해양스포츠체험교실, 제주관광대학의 윈드서핑 무료강습회, 2001년도 해양레저스포츠 지도자훈련과정(윈드서핑과정) 연수 참여자 및 학과목으로 윈드서핑을 이수하고 있는 학생들로 구성된 180명이었다. 본 연구에서 활용한 윈드서핑 만족도 조사지는 양명환(2000b)이 제작한 윈드서핑 참여만족 척도의 문항을 변형한 9문항의 간편형 척도를 사용하였다. 수집된 자료를 분석하여 얻은 주요 연구결과는 다음과 같다. 첫째, 활동만족, 생활만족, 시설만족, 대중화인지도, 정보활용 만족, 스포츠관광 기여도 변인에서 활동 참여수준에 따라서 유의한 차이를 보였다. 둘째, 성별에 따라서는 활동만족, 생활만족, 레저활용 만족변인에서 유의한 차이를 보였다. 구체적으로 활동만족의 경우는 남성이 여성보다 활동만족, 생활만족, 레저활용 만족변인에서 높게 나타났다. 셋째, 연령에 따라서는 신체적 건강만족과 정신건강 만족 변인 유의한 차이를 보였다. 넷째, 기량수준에 따라서는 활동만족, 생활만족, 정신건강 만족 변인들에서 유의한 차이를 보였다. 다섯째, 생활만족이 윈드서핑 활동만족을 가장 높게 예측할 수 있는 변인으로 나타났으며, 스포츠관광 기여도, 정보활용 만족도, 정신건강, 신체적 건강변인 순으로 활동만족을 유의하게 예측하였다.

여자 하반신의 체형 구성 인자에 관한 연구 : 만15~24세를 대상으로 from 15 to 24 years old women

문명옥 東義大學校 生活科學硏究所 1999 生活科學論集 Vol.3 No.-

This study was to analyze factors of the lower half of body form for cloth design which is intended to apply appropriate shape and capacity to cloth. 200 female subjects between 15 and 24 years old took part in this investigation. The result was as follows; 1. According to a comparison result of females' body shape between 15 and 17 years old with females between 18 and 24 years old, the value in length items get longer otherwise the value in circumferences of abdominal and ankle get slender. 2. There are three factors of body form selected by factor analysis. The 1st factor signifies the degree of fatness in the lower half of body. The 2nd factor signifies the height and length in the lower half of body. The 3rd factor signifies the height and length of the hip part.

한국 전통 이미지의 호텔용가운 디자인 개발에 관한 연구

문광희,문명옥 한국의류학회 2004 한국의류학회지 Vol.28 No.1

This study was conducted to redesign hotel robes based on Korean images. Hotel robes are furnished at many Korean hotels to increase the level of their gursts′ satisfaction toward their hotel services. The current hotel robe has several flaws in utility and design. Also, the robes register a low level on guests′ satisfaction while generating high cleaning cost. Consequently, improvements in the hotel robes′ material, motifs, style, and color were needed. The current one-piece, cotton towel weave robes used hotels are white and pale. Half of the hotel guests never wear the hotel robes during their stay, because they are unattractive and most Korean hotels provide a robe similar in design. Furthermore, these robes are undistinguishable from those found at other countries. Nevertheless, Korean hotel managers feel that the hotel robe is an important tool to express its hotel image and to increase guests′ satisfaction of their services. Most hotel managers and consumers agreed that it is necessary to design hotel robes based on Korean images. They believe that redesigning the hotel robes based on Korean images would induce hotel guests to wear them and at the same time feel part of the Korean traditional culture. The Korean traditional motifs of Taegug. Un (cloud), JuJag (phoenix), and Kumkwan (gold crown) were used in the new design of the hotel robes. The style of new hotel robes is the Korean traditional Pho of the Three Kingdoms. The colors of new hotel robes are white and pink. Forty-three examiners evaluated the newly designed robes for their aesthetic, symbolic and distinctional values. They concurred that the new robes were much better than the current ones.

발의 形態分析에 관한 연구(Ⅰ) : 발의 形 分類를 중심으로 on Classification of Foot Type

權瑛淑,文明玉 한국의류학회 1988 한국의류학회지 Vol.12 No.1

To classify the foot type, direct measurements, metatarso-phalanx angle and foot print angle of the right and left foot were measured and analyzed. The results are as follows. 1. The correlation coefficients between right and left foot were high degree, and except medial malleous height, the diffences between right and left foot are not significant. 2. The correlation coefficient among direct measurements of the foot are high degree, but the correlation coefficients between direct measurements and metatarso-phalanx angle and foot print angle are no or low degree. Therefore, to recognize the foot type, the direct measurements, metatarso-phalanx angle and foot print angle need to be measured independently. 3. According to foot width/foot length x100 which is the slender degree of foot, three groups are distinguished: one is slender type of which foot width/foot length x 100 is less than 40.14%, two is standard type of which foot width/foot length x 100 is 40.14%-44.30%, three is broad type of which foot width/foot length x 100 is more than 44.30%. 4. On the photographs metatarso-phalanx angle was measured, and two groups are distinguished: one is normal type of which the metatarso-phalanx angle is more than 160 and the other is the deformed type of which the metatarso-phalanx angle is less than 159° 5. By foot print angle 30° which need reformation of the foot, two groups are distinguished: one is normal foot print angle of which the angle is more than 30° and the other is flat foot print angle of which the angle is less than 30° 6. Classifications by foot width/foot length x100, metatarso-phalanx angle and foot print angle are put together, and then foot types are classified into 12 groups such as Table 11.

핵전이에 의한 Aspergillus oryzae var. oryzae와 Aspergillus nidulans 514의 잡종으로 부터 생산된 Xylanase의 특성

양영기,문명님,박형남,임채영 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

Aspergillus oryzae var oryzae 와 Aspergillus nidulans 514간의 xylanase 우수 생성 종간 형질전환체를 획득하고, 유전분석과 정제를 통하여 다음과 같은 결과를 얻었다. 1. 핵전이를 위한 원형질체 형성 및 재생 조건에서는 Novozym 234의 농도 1%, 삼투안정제는 0.6M (NH_4)_2 SO_4, 효소의 처리 시간은 90분 그리고 최적 pH는 5.8로 나타났다. 2. 핵전이에 의한 형질전환의 빈도는 3×10 exp (-5)~1×10 exp (-5)으로 비교적 낮은 편이었다. 3. 유전적 안정성, conidia의 크기, DNA 함량의 측정 그리고 핵염색의 결과 형질전환체의 핵형은 aneuploid로 추정되었다. 4. 형질전환체의 xylanase 활성은 모균주와 비교하여 1.1~1.4배 증가하였다. 5. 형질전환체중 xylanase 활성이 가장 높은 TAVD514-3을 정제하고 몇가지 특성을 조사하였는데 전체적인 회수율이 17%, 분자량은 32,000 그리고 효소 활성에 미치는 기질의 영향을 올 조사하였다. 6. 정제된 효소의 최적 pH와 온도는 각각 pH 9.0과 80℃로 나타났으며, 효소활성은 Mg^2+ 와 Mn^2+의 존재시에 증가하는 경향을 나타내었다. Inerspecific hybrids between Aspergillus oryzae var oryzae and A. nidulans 514 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated from conidiospores of the two strains were mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3×10 exp (-5)~1×10 exp (-5). From observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrids showed 1.1~1.4 fold higher xylanase activities than parental strains did. The xylanase of Aspergiilus sp. TAVD514-3 was purified and some of it's enzymatc characteristics were investigated. The enzyme was purified about 85 fold with an overall yield of 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and CM-sephadex A-50 ion exchange chromatography. The purified enzyme functions optimally at pH 9.0 and 80℃. The enzymatic activity was increased by the presence of Mg^2+ and Mn^2 ions.

大氣擴散模型의 最大着地點에서의 敏感度 分析에 관한 硏究 : RAM, ISCST 模型을 事例로

鄭長杓,朴明浩 慶星大學校 環境問題硏究所 1991 環境硏報 Vol.1 No.1

Air quality dispersion models(AQM) are widely used to control of air pollutants and to predict the distridutions of air pollutants But AQM have many problems and limitations, therefore it is necessary to validate models before use. So in this study, to improve the model accuracy, the sensitivity analysis of two short-term models(RAM,ISCST) is perforned. The conclusions obtained from this study were as follows. 1) RAM model shows generally lower concentration than ISCST models, and the concentration values of RAM and ISCST models are almost same over the maximum concentration of downwind distance. 2) It appears that the sensitivities of gas exit velocity, and wind speed, are significant. The sensitivities on the change of mixing And their Sensitivity coefficient of maximum concentration is considerably high. 3) The gas exit velocity, gas temperature which affect the plume rise, have some effect on the concentration values of each model within the downwind distance where final plume rise is determined. 4) The sensitivities on the change of mixing height is insignificant in each model in case of mixing height, is close to effective height of stack. 5) The sensitivities on the change of ambient air temperature stack exit velocity are lowest. And the sensitivity coefficient of two models with downwind distance show larger value in the near distance from source. 6) The sensitivities on the change of emission rate are not appear and that concentrations are computated relatively. 7) It appear that the maximum concentration of downwind distance on differential stack height are influenced than the other input parameter.

정신병원 간호사의 공정성 지각과 조직몰입이 이직의도에 미치는 영향

정수미,민순,강명근,류소연,박종,김기순 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objectives: The purpose of this study was to identify the influence of equality perception and organizational commitment on turnover intention of jobs among psychiatric hospital nurses. Method: Data were collected by questionnaire survey among 158 nurses working in 10 psychiatric hospitals from August 1st to September 30th. 2004. Results: The degree of turnover intention of jobs was significantly different by marital status, religion, qualification of psychiatric specialized nursing, monthly income and the location of hospitals. The correlation analysis showed that the turnover intention of jobs decreased as the equality perception and the organizational commitment increased. The hierachical multiple regression analysis showed that significantly related variables with turnover intention of jobs were religion, monthly income, equality perception and organizational commitment. The model explanation power was 53.1%. Conclusion: Because the equality perception explained 53.1% of variance of turnover intention among psychiatric nurses and organizational commitment was related with the turnover intention of nurses at psychiatric hospitals, activation strategy of organization must include the promotion strategy of equality perception and organizational commitment to decrease turnover intention of jobs among nurses.