http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Rapid preparation and characterization of chitosan nanoparticles for oligonucleotide
Mu-hua Cheng,Yao-xiong Huang,Han-jian Zhou,Zhi Liu,Jian-fang Li 한국물리학회 2010 Current Applied Physics Vol.10 No.3
Chitosan is regarded as one of the potential candidates as a gene carrier. However, the poor solubility of chitosan is the major limiting factor in its utilization as a gene carrier. The purpose of this study was to simplify the method of preparing the nanoparticles of chitosan linked with antisense oligonucleotide (asON). The main step was preparing the derivatives of chitosan phosphate (CSP) in order to easily dissolve in aqueous solution. The nanoparticles were formed using a simple mixed method for CSP and asON, and the nanoparticle’s forming condition was optimized so that the nanoparticle’s characterization could be examined. Results showed that it was simple to make the nanoparticles under the optimal condition of 2:1 M proportion of CSP and asON. The size of the nanoparticles was 102.6 ± 12.0 nm, its zeta potential was 1.45 ± 1.75, and the encapsulated ratio of the chitosan crosslinked the asON was 87.6 ± 3.5%. The infrared spectra and electron microscope displayed that chitosan may combine with the asON to form equirotal nanoparticles. In conclusion, it was simple and feasible to form chitosan nanoparticles for asON using the CSP, and the CSP can efficiently encapsulate asON.
Mu, Xiao-Yan,Dong, Xue-Li,Sun, Jie,Ni, Yu-Hua,Dong, Zhang,Li, Xi-Li,Sun, Er-Lian,Yi, Zhou,Li, Gao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
The effects of erlotinib combined with celecoxib in a lung cancer xenograft model were here explored with a focus on possible mechanisms. A xenotransplanted lung cancer model was established in nude mice using the human lung cancer cell A549 cell line and animals demonstrating tumour growth were randomly divided into four groups: control, erlotinib, celecoxib and combined (erotinib and celecoxib). The tumor major axis and short diameter were measured twice a week and after 40 days tissues were collected for immunohistochemical analyses of Bcl-2 and Bax positive cells and Western-blotting analyses for the epidermal growth factor recepto (EGFR), P-EGFR, and cyclooxygenase-2 (COX-2). Tumor size in the combined group was smaller than in the others (p<0.01) and the percentage of Bcl-2 positive cells was fewer in most cases (p<0.01), while that of Bax positive cells was greater than in the erlotinib and celecoxib groups (P>0.05). Western blotting showed decreased expression of P-EGFR and COX-2 with both erlotinib and celecoxib treatments, but most pronouncedly in the combined group (P<0.05). Simultaneous blockage of the EGFR and COX-2 signal pathways exerted stronger growth effects in our human xenotransplanted lung cancer model than inhibition of either pathway alone. The anti-tumor effects were accompanied by synergetic inhibition of tumor cell apoptosis, activation of p-EGFR and expression of COX-2.
A Self-Authentication and Deniable Efficient Group Key Agreement Protocol for VANET
( Mu Han ),( Lei Hua ),( Shidian Ma ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.7
With the rapid development of vehicular ad hoc Network (VANET), it has gained significant popularity and received increasing attentions from both academics and industry communities in aspects of security and efficiency. To address the security and efficiency issues, a self-authentication and deniable efficient group key agreement protocol is proposed in this paper. The scheme establishes a group between road side units (RSUs) and vehicles by using self-authentication without certification authority, and improves certification efficiency by using group key (GK) transmission method. At the same time, to avoid the attacker attacking the legal vehicle by RSUs, we adopt deniable group key agreement method to negotiation session key (sk) and use it to transmit GK between RSUs. In addition, vehicles not only broadcast messages to other vehicles, but also communicate with other members in the same group. Therefore, group communication is necessary in VANET. Finally, the performance analysis shows superiority of our scheme in security problems, meanwhile the verification delay, transmission overheard and message delay get significant improvement than other related schemes.
Isovitexin, a Potential Candidate Inhibitor of Sortase A of Staphylococcus aureus USA300
( Dan Mu ),( Hua Xiang ),( Haisi Dong ),( Dacheng Wang ),( Tiedong Wang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.9
Staphylococcus aureus causes a broad variety of diseases. The spread of multidrug-resistant S. aureus highlights the need to develop new ways to combat S. aureus infections. Sortase A (SrtA) can anchor proteins containing LPXTG binding motifs to the bacteria surface and plays a key role in S. aureus infections, making it a promising antivirulence target. In the present study, we used a SrtA activity inhibition assay to discover that isovitexin, a Chinese herbal product, can inhibit SrtA activity with an IC<sub>50</sub> of 28.98 μg/ml. Using a fibrinogen-binding assay and a biofilm formation assay, we indirectly proved the SrtA inhibitory activity of isovitexin. Additionally, isovitexin treatment decreased the amount of staphylococcal protein A (SpA) on the surface of the cells. These data suggest that isovitexin has the potential to be an anti-infective drug against S. aureus via the inhibition of sortase activity.
Error-Correcting Codes Scheme for Remote Control System
Wang, Mu kun,Yin, Xiao Hua,Zhao, Xian Ming,Li, Zheng Lian 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
How to rise the reliability of the data transmission is one of the main problem faced by modern digital communication designer. This paper studies the error-correcting codes being suitable for the channel existing both the random error and the burst error. A new error control scheme is given. The scheme is a concatenated coding system using an interleaved Reed-Solomon code with symbols over GF (2⁴) as the outer code and a Vitcrbi-decoded convolutional code as the inner code. As a result of the computer simulation, it is proved that the concatenated coding system has a output at a very low bit error rate (BER) and can correct a lot of compound error pattern. It is suitable for the serious disturb channel existing both the random error and the burst error. This scheme will be adopted for a remote control system
Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.
Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma
Cui, Mei-Hua,Hou, Xiao-Lin,Lei, Xiao-Yan,Mu, Fang-Hong,Yang, Gui-Bin,Yue, Lin,Fu, Yi,Yi, Guo-Xing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.
A Boundary Protection for Power Distribution Line Based on Equivalent Boundary Effect
Zhang Xin,Mu Long-hua 대한전기학회 2013 Journal of Electrical Engineering & Technology Vol.8 No.2
A boundary protection method for power distribution line based on equivalent boundary effect is presented in this paper. In the proposed scheme, the equivalent resonance component with a certain central frequency is sleeve-mounted at the beginning of protected zone. The ‘Line Boundary’ is built by using boundary effect, which is created by introducing impedance in the primary-side of line. The ‘Line Boundary’ is significantly different from line wave impedance. Therefore, the boundary protection principle can be applied to power distribution line without line traps. To analyze the frequency characteristic corresponding to traveling-waves of introducing impedance in the primaryside of line, distributed parameters model of equivalent resonance component is established. The results of PSCAD/EMTDC simulation prove the obvious difference of voltage high frequency component between internal faults and external faults due to equivalent resonance component, and validate the scheme.