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J.A. Morán Meza,J. Polesel-Maris,C. Lubin,F. Thoyer,A. Makky,A. Ouerghi,J. Cousty 한국물리학회 2015 Current Applied Physics Vol.15 No.9
Sharp Pt/Ir tips have been reproducibly etched by an electrochemical process using an inverse geometry of an electrochemical cell and a dedicated electronic device which allows us to control the applied voltages waveform and the intensity of the etching current. Conductive tips with a radius smaller than 10 nm were routinely produced as shown by field emission measurements through Fowler-Nordheim plots. These etched tips were then fixed on a quartz tuning fork force sensor working in a qPlus configuration to check their performances for both scanning tunneling microscopy (STM) and atomic force microscopy (AFM) imaging. Their sharpness and conductivity are evidenced by the resolution achieved in STM and AFM images obtained of epitaxial graphene on 6H-SiC(0001) surface. The structure of an epitaxial graphene layer thermally grown on the 6H-SiC(0001) (6 √3 × 6 √3 ) R30˚ reconstructed surface, was successfully imaged at room temperature with STM, dynamic STM and by frequency modulated AFM.
J. P. Morán-Lázaro,F. López-Urías,E. Muñoz-Sandoval,M. Courel-Piedrahita,A. Carreon-Alvarez,V. M. Rodríguez-Betancourtt,I. Zamudio-Torres,E. S. Guillén-López,A. Palafox-Corona 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.1
The acetone contained in human breath is of great interest for the health sector as it is a marker that allows to diagnoseand control diabetes in a non-invasive way. However, its concentration is extremely low. Therefore, high-performanceacetone sensors are still a challenge. With this in mind, MgCo 2 O 4 nanoparticles were synthesized using a microwaveassistedcolloidal route with subsequent calcination. Structural and morphological characterizations were done through varioustechniques. The MgCo 2 O 4 sensor was fabricated with the sample calcined at 500 °C. The sensing results showed that thesensor could detect acetone vapors ranging from 0.5 to 50 ppm at an optimum operating temperature of 250 °C with a highresponse, repeatability, stability, and selectivity. These sensing characteristics revealed that MgCo 2 O 4 could be used as a newsensor material to detect acetone in exhaled human breath.
Acute and a 28-repeated dose toxicity study of commercial oleoresin from Copaifera sp. in rodents
Marina Alves Coelho Silva,Dorcas Fernandes dos Anjos Melo,Sayonara Ay Moré de Oliveira,Alessandro de Carvalho Cruz,Edemilson Cardoso da Conceição,José Realino de Paula,Ruy de Souza Lino Junior,Luiz Ca 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.4
Copaifera spp. (copaiba) oleoresin is traditionally used as a medicinal compound since the 16th century, which is primarily indicated for its anti-inflammatory properties being widely sold in free fairs and medicinal herbal houses in Brazil. However, copaiba oleoresin toxicity information is still limited. In this study, we aimed to investigate the acute (in mice and rats) and 28 day—repeated doses (in rats) oral toxicity of commercial copaiba oleoresin. In the acute toxicity test, copaiba oleoresin oral administration at 2000 mg/kg dose did not induce mice or rat lethality. In the subacute toxicity tests, we administered copaiba oleoresin at daily doses of 25, 50, or 100 mg/kg for 28 days. We observed no toxicological effects in body weight gain, feed and water intake, gross necropsy, relative organ weight, histopathology, hematology, or biochemistry parameters. At the three copaiba oleoresin tested doses, we observed an alkaline phosphatase reduction, which might indicate hepatic protection. In conclusion, the commercial copaiba oleoresin has a low risk of toxicity and did not induce treatment-related adverse effects after short-term daily exposures to a dose two thousand times higher than folk use, showing safety doses for further pharmacological investigations.
Anaerobic Co-digestion of Swine Manure with Energy Crop Residues
María José Cuetos,Camino Fernández,Xiomar Gómez,Antonio Morán 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5
Anaerobic co-digestion involves the treatment of different substrates with the aim of improving the production of biogas and the stability of the process. In this research, co-digestion of swine manure (SM) and energy crop residues (ECRs) was studied. The mixtures evaluated contained SM combined with maize (Mz), rapeseed (Rs) or sunflower (Sf) residues. Batch and semi-continuous experiments were performed to determine methane (CH4) yields and the behavior of reactors while co-digesting agricultural wastes. Three different proportions of ECRs were tested in batch experiments for co-digestion with SM: 25, 50, and 75% volatile solids (VS). On the basis of the results obtained from batch tests, a mixture with a 50% ECR content was selected for the second stage of the study. Mesophilic reactors with a 3 L working volume were used for semicontinuous experiments. The hydraulic retention time (HRT)was set at 30 days and the reactors were kept under these operational conditions over four HRTs. The addition of ECR to the co-digestion system resulted in a major increase in the amount of biogas produced daily. The highest biogas yield was obtained when co-digesting Rs (3.5 L/day),although no improvement was observed in specific gas production from the addition of the co-substrate.
Characterization of CBD-CdSe1-ySy deposited at low-temperature for photovoltaic applications
J.I. Contreras-Rascón,J. Díaz-Reyes,J.E. Flores-Mena,M. Galvan-Arellano,L.A. Juárez-Morán,R.S. Castillo-Ojeda 한국물리학회 2015 Current Applied Physics Vol.15 No.12
We present the structural and optical characterization of cadmium selenide sulphur (CdSe1-ySy) deposited by chemical bath deposition (CBD) technique at low-temperature (20 ± 2 ℃). The sulphur molar fraction is varied from 0 to 42.13%. The chemical stoichiometry is estimated by energy-dispersive X-ray spectroscopy (EDS). The CdSe1-ySy shows hexagonal wurtzite crystalline phase, which was found by X-ray diffraction (XRD) analysis and it was confirmed by Raman spectroscopy. The average grain size of the CdSe1-ySy films was ranged from 1.20 to 1.68 nm that was determined by Debye-Scherrer equation from W(002) direction and it was confirmed by high resolution transmission electron microscopy (HRTEM). This average grain size indicates a high quantum confinement because of it is smaller than the Bohr radii of CdS (2.8 nm) and CdSe (4.9 nm). Raman spectra show two dominant vibrational bands about 208 and 415 cm-1 associated at CdSe-1LO-like and CdSe-2LO-like. By transmittance measurements at room temperature are found that the optical band gap energies vary from 1.86 to 2.16 eV in the range of investigated sulphur molar fraction. Room temperature photoluminescence shows radiative bands in the visible range and a dominant band in the UV range, approximately 3.0 eV, which can be associated with a radiative transition, bound exciton to donor impurity.
Structural and optical characterization of wurtzite type ZnS
J. Díaz-Reyes,R.S. Castillo-Ojeda,R. Sánchez-Espíndola,M. Galván-Arellano,O. Zaca-Morán 한국물리학회 2015 Current Applied Physics Vol.15 No.2
ZnS films were grown on (001) GaAs substrates at different temperatures by RF magnetron sputtering. The ZnS chemical stoichiometry was determined by Energy-dispersive X-ray spectroscopy (EDS), besides it allowed to find the residual impurities, mainly oxygen. The X-ray diffraction (XRD) analysis and Raman scattering reveal that ZnS deposited thin films showed hexagonal wurtzite crystalline phase. The films average crystallite size range was from 8.15 to 31.95 nm, which was determined using the Debye eScherrer equation for the peak W(101). Besides an experimental study of first- and second-order Raman scattering of ZnS films is made. An energy level diagram involving oxygen traps and interstitial sulphur ions is used to explain the origin of the radiative transitions observed in the room temperature photoluminescence (PL) spectra.
Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
Kotasová Hana,Capandová Michaela,Pelková Vendula,Dumková Jana,Koledová Zuzana,Remšík Ján,Souček Karel,Garlíková Zuzana,Sedláková Veronika,Rabata Anas,Vaňhara Petr,Moráň Lukáš,Pečinka Lukáš,Porokh Volo 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5
BACKGROUND The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. METHODS By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. RESULTS These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. CONCLUSION Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
Somoza Maria Luisa,Prieto-Moreno Pfeifer Ana,Martín-Pedraza Laura,Victorio Puche Laura,Esteban Rodríguez Angel,Blanca-López Natalia,Eva Fernández González Abel,Fernández-Caldas Enrique,Morán Morales M 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.6
The most important peach fruit allergen is Pru p 3, followed by Pru p 1, Pru p 4, and Pru p 7. We aimed to assess their role in subjects with peach fruit-induced allergy (anaphylaxis and OAS) and compare skin prick tests (SPT) vs. specific immunoglobulin E (sIgE) for predicting anaphylaxis. We also selected a control group. SPT included prevalent inhalant and plant food allergens plus peach peel extract. The sIgE to Pru p 1, Pru p 3, Pru p 4, and Pru p 7 were quantified. Compared with controls (n = 42), cases (n = 41) were younger (P = 0.003), more frequently female (P < 0.05) and had higher SPT positivity to peach peel (44% vs. 2.4%, P < 0.0001). There were significant differences in sensitization to several pollens: Olea europaea, Artemisia vulgaris, Prunus persica, Platanus acerifolia (all P < 0.001); and fruits: apple (P < 0.04), peanut (P < 0.002), tomato (P < 0.005), and melon (P < 0.05). Pru p 3 sIgE was detected in 61% of all cases (85% anaphylaxis and 38% OAS; P < 0.01 each) and 5% of controls (P < 0.001). Pru p 4 sIgE was present in 19% of cases and 7% of controls. The sIgE to Pru p 1 and Pru p 7 were not found. The odds ratio to predict anaphylaxis for peach peel SPT was 113 (confidence interval [CI], 20–613; P < 0.0001); for sIgE to Pru p 3, 22 (CI, 5.3–93; P < 0.0001); and for SPT positivity to selected plant food allergens, 5 (CI, 1–19; P < 0.05). In our study group, SPT with peel peach extract was a better predictor of anaphylaxis than Pru p 3 sIgE or other variables considered. The role of sIgE to Pru p 1, Pru p 4, and Pru p 7 seemed negligible.