HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

난소의 성숙 낭성 기형종에서 발생한 원발성 유암종 : 1예 보고 A case Report

조향정,민부기,문형배 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.1

저자들은 우측 난소의 성숙 낭성 기형종에서 발생한 원발성 유암종 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 본 예는 수술 후 상부 소화기 내시경검사, 대장 내시경 검사 및 복부와 골반에 대한 전산단층촬영을 시행하였으나 특이 소견은 없었고 현재 6개월정도 추적 관찰중이다. The most common malignant change in cystic teratoma is squamous cell carcinoma, followed by carcinoid tumor and adenocarcinoma. Ovarian carcinoid tumor can be seen in the ovary as a metastasis of a tumor located in the gastrointestinal tract or elsewhere, as a component of adult cystic teratoma, or as a primary pure neoplasm of the ovary. The prognosis in primary carcinoid tumors (whether pure or as a component of cystic teratoma) is very good, whereas metastatic carcinoids have a poor outcome. We report a case of a primary carcinoid tumor arising in a mature cystic teratoma of the ovary that developed in a 38-year-old woman with a brief review of literature.

Scrub Typhus during Pregnancy

정문현,강영숙,장호열,이향미 대한감염학회 1996 감염 Vol.28 No.5

임신 기간 중 발생한 감염증은 산모뿐만 아니라 태아에게도 영향을 미치며, 또한 치료약에 의한 영향도 고려해야 하기 때문에 치료약의 선택이 어려울 때가 있다. 쯔쯔가무시병의 임신에 대한 영향은 잘 알려져 있지 않으며, 임신 중 발생한 쯔쯔가무시병은 매우 드물며 국내에서는 아직 보고가 없다. 저자들은 임신 15주에 발열을 주소로 내원한 환자에서 혈청학적으로 증명된 쯔쯔가무시병을 erythromycin으로 치료하였고, 신생아를 1년 이상 경과 관찰하였으나 이상을 발견할 수 없었다. Certain infectious diseases acquired during pregnancy can cause adverse outcome to mother and fetus, and also raise the problem to choose appropriate antibiotics. Cases of scrub typhus contracted during pregnancy are rare and the risk of adverse effects to the fetus is unclear. We report a case of scrub typhus in a pregnant woman in the fifteenth week of gestation, who was a 27-year-old Korean woman presented with fever and an eschar. She was managed empirically with erythromycin, resulting in complete recovery, and scrub typhus was confirmed by seroconversion. The child have not shown any abnormalities on delivery and during the one-year follow-up.

Proteus 균종에서 플라스미드 pBluescript의 전기적 재결합에 관한연구

최향경,조지현,문영희 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

In order to genetic analysis of microorganisms, electroporation method was applied to induce electrotransformation of two strains of Proteus mirabilis (API code: 0536000 and 0737000). isolated from patients at Wonkwang University Hospital. In this study, we tried to evaluate the effect of parameters, ie amount of competent cell and plasmid DNA(pBluescript) on the survival of transformant and transformation efficiency and examine antibiotic susceptibility patterns and biochemical properties of tested strains before and after electroporation. The results were as follows: 1. The effect of amount of competent cells and plasmid DNA to transformation efficiency were different between strains. But the highest efficiency was noted at the amount of pBluescript 20ng. 2. In order to confirm the transformant, pBluescript before and after electroporation were isolated from colonies on the LB agar containing ampicillin and electrophoresed. Two plasmids showed same sized DNA. 3. Antibiotic susceptibility to ampicillin was changed from susceptible to resistant in both strains and to carbenicillin. changed from susceptible to intermediate.

신생아 이송에서의 저체온증

김향숙,정윤석,조준필,박문성,배기수 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Back Ground : Hypothermia is a condition that can lead to serious complications and even to death in newborn. Although the temperature control is essential in neonatal care, it is often neglected during urgent transfer from local private hospitals. The purpose of this study is to evaluate the incidence and clinical outcomes of transfer induced neonatal hypothermia. Subjects and Method : In this retrospective study, subject is limited to transferred outborn babies with age less than 27 hours from June 1996 to May 1999. A total of 3,086 patients were admitted in NICU during the study period and inborn and outborn babies were 1,743(56%) and 1,343(44%) respectively. Among the 1,343 outborn babies, 212 babies were transferred from the private hospital within 24 hours of birth and were eligible for the study. Rectal temperature on arrival, transfer time, birth weight, gestational age, initial arterial gas study, and clinical outcome were compared. Statistical analysis has been done with chi-sqaure test and multiple logistic regression analysis. Results : There were 84 cases(39.6%) hypothermia(<36℃) among the 212 babies and the rate of hypothermia in transfered babies have not decreased over the study period. There were significantly more hypothermia in lower gestational age (less than 28 weeks) and lower birth weight (less than 2,000gm) caused hypothermia significantly more than normal gestational age or normal range of birth weight. And time interval (less than 12 hour) from birth to arrival at emergency department was also significant factor in hypothermia. The mortality rate was three fold higher in hypothermia than normothermic or hyperthermic babies. Conclusion: This study shows that hypothermia during neonate transportation is a major cause of neonatal mortality and morbidity in prehopital care. Body temperature control during transport of neonates under 24 hours of age should be emphasized for the better outcome of treatment.

Eethyl benezylidencyanoacetate 유도체에 대한 thiophenol 첨가 반응의 속도론적 연구

맹주양,黃善岩,金文執,崔元植,朴茂淳 順天鄕大學 1991 순천향대학논문집 Vol.14 No.1

자궁경부암종에서 신생혈관 형성과 증식세포 핵항원 및 Ki-67 표지지수의 예후적 의의

박찬필,박문향,이승연 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.1

상악골 침범 소견을 동반한 선천성 침윤 지방종

허경회,이향옥,이서영,최형길,하병각,문제운,이삼선 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.37 No.2

Infiltrating lipoma is a rare mesenchymal neoplasm that, in spite of benign nature, characteristically infiltrates adjacent tissues and tends to recur after surgery. It has a predilection for the extremities and the trunk and is extremely rare in the head and neck region. We present a case of congenital infiltrating lipoma of the face, describing the intrabony invasion and osseous dystrophy as well as the soft tissue changes seen on plain radiographs and magnetic resonance imaging.

Eethyl benezylidencyanoacetate 유도체에 대한 thiophenol 첨가 반응의 속도론적 연구

朴茂淳,金文執,崔元植,黃善岩,孟柱煬 순천향대학교 1991 논문집 Vol.14 No.1

Reaction of benzaldehyde derivatives with ethylacetate afforded ethylbenzylidenecyanoacetate. Especially, the crystal structure of ethyl p-methoxybenzylidenecyanoacetate were demonstrated by X-ray diffrection method. The crystal structure is only exist in E-form. The kinetic studies on the mechanism of nucleophilic addition of thiophenol to ethyl benzylidenecyanoacetate have been investigated by UV-spectrophotomery and the rate equation which can be applied over wide pH range was obtained. From the rate equation, effect of generalbase and substituent, the fellowing reaction proposed. Below pH 3.0, thiophenol added to the double bond, while above pH9.0 thiophenoxide ion added to the double bond. In the range of pH from 3.0 to 9.0, these reaction occurred competitively.

국내 Fabry disease 환자의 a-Galatosidase A 유전자 돌연변이 검색

박기범,최지혜,강윤성,김선미,정향민,문영준,이광호 中央大學校 基礎科學硏究所 2001 基礎科學硏究所 論文集 Vol.15 No.-

Fabry disease(FD) is an X-linked recessive lysosomal disorder caused by a deficiency of a-galactosidase A(a-Gal A), localized at Xq22. Besides onset of pain and paresthesias in the extremities, FD was diagnosed by absence of a-Gal A activity. In this experiment the a-Gal A activity of Korean FD patients was spectrometrically analysed using an artificial substate, 4-Mrthylumbellifery1-a-D-galactoside. As expected, no a-Gal A activity was detected in lymphocytes and lymphoblastoid cells from FD patients. To screen the mutation in their a-Gal A genes, we performed single strand conformation polymorphism(SSCP) and PCR-direct sequencing form seven a-Gal A exons. The nonsense mutation was identified both in classically affected hemyzygotes and a heterozygote. They showed the C to T transition at nucleotide number 11,002, resulting in a arginine-to-stop(R342X). This result will be applicable for pre- and neonatal detection of FD and to define the genotype/phenotype correlation.