Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

H₂ pressure swing adsorption for high pressure syngas from an integrated gasification combined cycle with a carbon capture process

Moon, D.K.,Lee, D.G.,Lee, C.H. Applied Science Publishers 2016 APPLIED ENERGY Vol.183 No.-

<P>The integrated gasification combined cycle (IGCC) process, possessing high efficiency and environmental advantages, produces H-2-rich syngas at high pressures (30-35 bar) after capturing CO2. Since the syngas pressure is very high for conventional PSA processes, development of an efficient PSA process at the pressure conditions is required for H-2 production. In this study, the H-2 PSA process for IGCC syngas was developed experimentally and theoretically. Breakthrough and PSA experiments using activated carbon or activated carbon/zeolite LiX were performed at 25-35 bar by using a five-component hydrogen mixture (H-2:CO:N-2:CO2:Ar = 88:3:6:2:1 mol%) as a simulated syngas. The overall PSA performance was evaluated in terms of the purity, recovery and productivity of H-2 product. According to the results from using single or layered beds, the two-bed PSA process produced 99.77-99.95% H-2 with 73.30-77.64% recovery experimentally. A four-layered bed PSA at 35 bar was able to produce 99.97%-purity H-2 with 79% recovery, and it contained Ar and N-2 impurities. The quality of tail gas from the PSA process could be used for the gas turbine without losing H-2 and CO. A rigorous mathematical model that included mass, energy, and momentum balances was employed to elucidate the dynamic behaviors and separation performance of the adsorption bed and PSA process. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Effect of fermentation conditions on biohydrogen production from lipid-rich food material

Kim, H.,Moon, S.,Abug, A.,Choi, S.C.,Zhang, R.,Oh, Y.S. Pergamon Press ; Elsevier Science Ltd 2012 International journal of hydrogen energy Vol.37 No.20

Among the basic components of organic materials, such as carbohydrate, protein, and lipid, the hydrogen yield of carbohydrate fermentation has been reported to be significantly higher than that of lipid. This study used lard as a model organic matter for lipid and investigated its H<SUB>2</SUB> production potential in batch anaerobic fermentation experiments under various combinations of stirring and CO<SUB>2</SUB>-scavenging conditions. A significant increase in the hydrogen yield was observed in both CO<SUB>2</SUB>-scavenging and stirring conditions; the CO<SUB>2</SUB>-scavenging condition yield was 2.9 times higher than the stirring condition (116.7 and 40.3 mL H<SUB>2</SUB>/g volatile solid [VS], respectively), which was much greater than reported previously. A maximal hydrogen yield of 185.8 mL H<SUB>2</SUB>/g VS was obtained in the presence of both CO<SUB>2</SUB>-scavenging and stirring, and the H<SUB>2</SUB> content of the total biogas was as high as 99% (v/v). In addition, there was less H<SUB>2</SUB> and more CH<SUB>4</SUB> production in the absence of CO<SUB>2</SUB>-scavenging and/or stirring, which suggests that the consumption of H<SUB>2</SUB> and CO<SUB>2</SUB> for methanogenesis was the major mechanism of the poor hydrogen yield from lipid. The volatile fatty acids in all the tests consisted primarily of valeric (47.2-54.9%) and propionic acids (26.6-30.3%), and higher concentrations of these acids remained in the fermentation liquid without CO<SUB>2</SUB> removal. These results suggest that lipid-rich food waste is a potential source for H<SUB>2</SUB> production if the fermentation process is optimized to minimize the partial pressure of CO<SUB>2</SUB> and H<SUB>2</SUB> and restrain the activities of H<SUB>2</SUB>-consuming bacteria.

볏짚의 사료가치 증진을 위한 적정 알카린 H2O2 처리 수준에 관한 연구

문양수(Y . S . Moon),하종규(J . K . Ha),고종열(J . Y . Ko),최연호(Y . H . Choy),조경훈(G . H . Cho),최윤재(Y . J . Choi),한인규(I . K . Han) 한국축산학회 1990 한국축산학회지 Vol.32 No.10

This study was carried out to determine the adequate levels of alkaline hydrogen peroxide treatment for the improvement of nutritive value of rice straw. In vitro digestibility and chemical analysis after several treatments were measured. Treatment variables were soaking time(12, 24, 48 and 96 hrs), temperature(5, 25, 50 and 75℃) of alkaline H₂0₂ solution, H₂0₂ concentration (0.5, 1, 2, 3 and 4%) and substrate /solution ratio (1, 2, 3, 4 and 5g/ml). The results obtained were summarized as follows. 1. The DM digestibilities or rice straw treated with H₂O₂ for 24 and 48 hours were higher than those of the others(p$lt;0.05). There was no differ ences when the rice straw was treated for 12, 72 or 96 hours. Cell wall contents were not affected by the time of treatment. 2. The DM digestibilities were higher at pH 11.5 or above(p$lt;0.05) and was decreased as pH declined. When rice straw was treated with H₂O₂ at pH 11.5 or higher, NDF, ADF and cellulose contents were increased. However, lignin content and DM recovery percentages were decreased. 3. The DM digestibilities were not influenced by the ratio of straw versus liquid. The contents of NDF and cellulose were not affected up to the ratio of 4g rice straw/50m1, but were decreased at 5g rice straw/50m1. Lignin content and DM recovery percentages, however, were increased at 5g rice straw /50m1. 4. The DM digestibilities were not different among treatments at 5, 25 and 50℃. However, the rice straw treated at 75℃ showed lowest digestibility of all treatment (p$lt;0.05). The content of NDF, ADF and cellulose was increased by increasing temperature. However, lignin content and DM recovery percentages were decreased by increasing temperature. 5. The AHP treated rice straw had higher content of ADF and cellulose, and lower content of hemicellulose and lignin as the concentration of H₂O₂, increased. But hemicellulose, lignin and DM recovery percentages were decreased. The NDF content was not different among five different concentrations of H₂0₂ solution. In conculsion, based upon the results of present experiments the most desirable method is to soak rice straw in 1% alkaline H₂0₂ solution at pH 11.5, at room temperatrve (25℃), for 24∼48 hours and at the ratio of 4g rice straw /50m1 solmtion.

A new class of COX-2 inhibitor, rutaecarpine from Evodia rutaecarpa

Moon, T. C.,Murakami, M.,Kudo, I.,Son, K. H.,Kim, H. P.,Kang, S. S.,Chang, H. W. 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

Objective and Design: We investigated the effect of a new class of COX-2 inhibitor, rutaecarpine, on the production of PGD, in bone marrow derived mast cells(BMMC) and PGE_(2) in bone marrow derived mast cells (BMMC) and PGE_(2) in COX-2 transfected H다293 cells. Inflammation was induced by λ-carrageenan in male Splague-Dawley(SD) rats. Material: Rutaecarpine(8, 13-Dihydroindolo[2', 3':3,4]pyridol[2,1-b]quinazolin-5(7H)-one) was isolated from the fruits of Evodia rutaecarpa. BMMC were cultured with WEHI-3 conditioned medium. c-Kit ligand and IL-10 were obtained by their expression in baculovirus. Methods: The generation of PGD_(2) and PGE_(2) were determined by their assay kit. COX-1 and COX-2 protein and mRNA expression was determined by BMMC in the presence of KL, LPS and IL-10. Treatment: Rutaecarpine and indomethacin dissolved in 0.1% carboxymethyl cellulose was administered intraperitoneally and, 1h later, λ-carrageenan solution was in-jucted to right hind paw of rats. Paw volumes were measured using plethysmometer 5h after λ-carrageenan injection. Results: Rutaecarpine inhibited COX-2 and COX-1 dependent phases of PGD_(2) generation in BMMC in a concentration-dependent manner with an IC_(50) of 0.28 μM and 8.7μM, respectively. It inhibited COX-2-dependent conversion of exogenous arachidonic acid to PGE_(2) in a dose-dependent manner by the COX-2-transfected HEK293 cells. However, rutaecarpine inhibited neither PLA_(2) and COX-1 activity nor COX_(2) protein and the mRNA expression up to the concentration of 30μM in BMMC, indicating that retaecarpine directly inhibited COX-2 activity. Furthermore, rutaecarpine showed in vivo anti-inflammatory activity on rat λ-carrageen-an induced paw edema by intraperitoneal administration. Conclusion: Anti-inflammatory activity of Evodia rutaecarpa could be attributed at least in part by inhibition of COS-2.

Mucosal immunization with recombinant influenza hemagglutinin protein and poly gamma-glutamate/chitosan nanoparticles induces protection against highly pathogenic influenza A virus

Moon, H.J.,Lee, J.S.,Talactac, M.R.,Chowdhury, M.Y.E.,Kim, J.H.,Park, M.E.,Choi, Y.K.,Sung, M.H.,Kim, C.J. Elsevier Scientific Pub. Co 2012 Veterinary microbiology Vol.160 No.3

Intranasal administration of recombinant influenza hemagglutinin (rHA) antigen or inactivated virus with nanoparticles (NPs) composed of poly-γ-glutamic acid (γ-PGA) and chitosan which are safe, natural materials, and able to target the mucosal membrane as a mucosal adjuvant, could induce a high degree of protective mucosal immunity in the respiratory tract. Intranasal immunization with mixture of rHA antigen or inactivated virus and γ-PGA/chitosan nanoparticles (PC NPs) induced not only a high anti-HA immunoglobulin A (IgA) response in lung and IgG response in serum, including anti-HA neutralizing antibodies, but also an influenza virus-specific cell-mediated immune response. Also, PC NPs could function as a potential mucosal adjuvant when it was compared with the well-known mucosal adjuvant, cholera toxin (CT). Intranasal administration of rHA antigen or inactivated virus with PC NPs protected mice against challenge with a lethal dose of the highly pathogenic influenza A H5N1 virus. These results suggested that application of PC NPs with a subunit antigen of influenza produced by prokaryotic expression system provides several solutions to the current problems of the influenza vaccines using inactivated influenza virus. Moreover, our finding about a sufficient function of PC NPs to elevate vaccine efficacy led us to consider that it can be useful in clinical applications as a potent mucosal adjuvant with safety.

H대학교 아음속 풍동 개념설계

장조원,전창수,김문상,이열,문희장,송병흠,김학봉,Chang, J.W.,Jeon, C.S.,Kim, M.S.,Lee, Y.,Moon, H.J.,Song, B.H.,Kim, H.B. 한국항공운항학회 2005 한국항공운항학회지 Vol.13 No.4

A closed-circuit type wind tunnel is designed, which has a test section with the dimensions $1.2(W){\times}1.2(H){\times}3.4(L)$. A subsonic wind tunnel is designed to improves educational circumstances and promote ground tests. It is constituted of an exchangeable test section, first and second diffusers, a fan, a settling chamber, a contraction, and 4 corners. The maximum velocity in the test section is 70m/s and the contraction ratio is 6.25:1. Input power in the wind tunnel is about 96.1 kw (128.8 hp) and its energy ratio is 3.89. It has the dimension of about $7.4(W){\times}3.6(H){\times}21.7m(L)$. The wind tunnel designed in this investigation will be an effective educational and investigational equipment.

H형강 롤포스 예측 모델 개발

정현석(H. S. Jung),위창현(C. H. Wee),문홍길(H. K. Moon),서경호(K. H. Seo),김태형(T. H. Kim),배병철(B. C. Bae),이영석(Y. S. Lee) 한국소성·가공학회 2023 한국소성가공학회 학술대회 논문집 Vol.2023 No.11

난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

Anti-inflammatory effects of extract from Haliotis discus hannai fermented with Cordyceps militaris mycelia in RAW264.7 macrophages through TRIF-dependent signaling pathway

Joung, H.J.,Kim, Y.S.,Hwang, J.W.,Han, Y.K.,Jeong, J.H.,Lee, J.S.,Moon, S.H.,Jeon, B.T.,Park, P.J. Academic Press 2014 FISH AND SHELLFISH IMMUNOLOGY Vol.38 No.1

In this study, Haliotis discus hannai (H. discus hannai) fermentation was attempted with Cordyceps militaris (C. militaris) mycelia using a solid culture. We tried to ferment H. discus hannai to determine the optimal conditions fermentation with regards to its anti-inflammatory effects. The extracts of H. discus hannai fermented with C. militaris mycelia (HFCM-5) showed higher nitric oxide inhibitory effects than H. discus hannai and C. militaris alone. HFCM-5 also decreased pro-inflammatory cytokines, TNF-α and IL-6 in a dose-dependent manner. HFCM-5 did not affect the MyD88-dependent pathway, but decreased phosphorylation of IRF3 and STAT1 which are involved in TRIF-dependent pathway. Taken together, our results suggest that HFCM-5 exerts its anti-inflammatory effects via TRIF signaling pathway and could potentially be used as a functional food in the regulation of inflammation.