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FIBRIN GLUE가 이식 피부의 접착 및 생착에 미치는 효과
백무현,홍종현,박언섭,민대홍,김승홍,김미경 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.2
The ability of a skin graft to adhere initially to a wound a bed is dependent on the formation of a stable fibrin bond the elastin in the graft and the elastin in the bed. the Factors preventiong such adhesion most commonly include bleeding, infection, mechanical disturbances of the adhesive mechanism, i.e., motion. This study was conducted to evaluate the adhesive effect of fibrin glue in the full-thickness skin grafts at 3,5,30,120minute and 8 hours after gluing in mice and the effect of fibrin glue on the skin graft take at the 3,7,15post operative days in rabbits. The result were as follows; (1) Mechanical testing demostrated that the shear adhesive strength of non-fibrin gluing control group was found to be 2.84m²(SD=2.68) after 3minutes of fixation At, 5minutes, the adhesive strength was 9.78gm/㎠(SD=2.68) after 3minutes of fixation. At 5minutes, the adhesive strength was 9.78gm/㎠(SD=5.61);at 30minutes, 30.78gm/㎠(SD=5.42); at 2hours, 53.14gm/㎠(SD=11.50); and at 8 hours, 108.08gm/㎠(SD=15.78) and that the shear adhesive strength of fibrin glue applied group was found to be 17.50gm/㎠(SD=5.79) after 3minutes of of fixation(after gluing). AT 5minutes, the adhesive strength was 24.30gm/㎠(SD=3.57); at 30minutes, 105.48gm/㎠(SD=15.27); at 2hours, 167.54gm/㎠(SD=58.12); and at 8hours, 286.22gm/㎠(SD=28.27). These results in shear adhesive strength were a significantly different between the experimental and the control groups at early skin grafts adherence. (2) The histologic examination did not demonstrate significant difference in healing process when the fibrin glue applied group were compared with the control group. With the above results, fibrin glue improved in early graft adherence without interference of the take of graft.
배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성
홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.
홍성렬,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2
JunB cDNA was inserted into a beta-actin promoter expression vector naming LK4JunB. The LK4JunB was introduced into F9 cells by electroporation. Some 5,000 clones resistant to G418 were identified. Three of 12 G418 resistant clones expressed elevated levels of JunB RNA. The two highest expressing isolates were subcloned. All of eight subclones of the first isolate were found to express elevated levels of JunB RNA ensuring the homogeniety in expression of the JunB RNA among subclones. The overexpression of JunB on the retinoic acid induced differentiation of F9 cells was assessed by RNA analysis of cells treated with the inducer for various lengths of time. Elevated JunB expression was evident in both experimental clones regardless of the time of treatment with retinoic acid. In the parental F9 cells, elevated JunB RNA was detected after three days of treatment but not before.
네오마이신 인산기전위효소 유전자의 F-9 세포 內 형질전환
홍성렬,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2
쥐 배종양세포, F-9, 22에 aminoglycoside 계통의 항생제 G-418 저항성을 갖도록 형질전환 시키었다. 즉, aminoglyucoside phosphotransferase 유전자를 갖고 있는 포유동물세포 발현 백터 pHβ APr-1-neo를 F-9, 22 세포에 electrophoration 법을 이용하여 transfection시키었다. 이 형질전환된 세포를 여러차례 subcultrue한 다음 neo 유전자 저항 능력이 소멸되지 않고 아주 안정하게 유지됨을 보았다. neo유전자로 형질전환된 클론은 RNAse protection assay로 관찰하였으며, 이를 위하여 BSpMC1rp를 합성하였다. A Plasmid PH βAPr-1-neo containing phosphotransferase gene (neo) was introduced into murine embryonal carcinoma (EC) cells (F-9, 22) by electroporation (250 volts/cm, 10 millisecond).. Colonies resistant to the drug G-418 were selected as plausible clones transfected by the neo. Elevated levels of the neo in the putative clones were confirmed by RNAse protection experiments, one of which named "F-9/neo". A 241 bp fragment of neo cDNA (EcoRl-Narl segment of the plasmid pMClneoA) was ligated to generate BSpMC1rp for the neo riboprobe synthesis. Transcription of EcoRl cut BSpMC1rp in the presence of 32pGTP yielded a 384 by probe, of which 284 by were protected by endogenous neo mRNA in the F-9 /neo clone.
洪茂昌,申玟圭,禹太律 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1
We induced brain ischemia using middle cerebral artery occlusion(MCAo) and studied the results of walking initiation and circling test, posture reflex test, water bath swimming test to characterize the motor dysfunction and establish the model of stroke by MCAo for oriental medicine. Analysis of total rotation angle during 1 min. swimming showed significant motor dysfunction in occlusion group(3hrs, 6hrs, 9hrs, 24hrs after MCAo). Analysis of rectal rotation number during 1 min. swimming showed significant difference between control group and occlusion group(3hrs, 6hrs, 9hrs, 24hrs after MCAo). Analysis of posture reflex test showed significant difference between control group and occlusion group(3hrs, 6hrs, 9hrs, 24hrs, after MCAo). Significant motor dysfunctions are shown at start time in 24hrs, at inner circle time and outer circle time in 9hrs, 24hrs, at contalaterl or ipsilateral circling number or 180°turn in 3hrs, 6hrs, 9hrs, 24hrs during walking initiation and circling test. Results revealed that brain ischemia by intraluminal method was good at stroke model for oriental medicine which chiefly reveals motor dysfunction by paralysis of one side and behavior index suggested in this article could be used as a useful index for assessment of motor dysfunction.
洪仁杓,金武剛 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1
In order to observe histologically the injurious effects of mice exposed to sulfur dioxide, mice were exposed to 25 and 50 ppm, of sulfur dioxide for 10 days respectively. The results obtained are as follows : 1. In the clinical signs, the exposed mice become restless and severely dyspnic, rubbing their noses and eyes immediately after the biginning of exposure to sulfur dioxide. 2. Microscopical changes in the upper--and lower-respiratory tract were observed focal loss of cilia and goblet cell hyperplasia after 2 days of exposure to 25 and 50 ppm. , more extensive loss of cilia, disappearance of goblet cells, necrosis and extrusion of cells, and irregular rows of nuclei of basal, supporting, and olfactory cells after 4 and 6 days of exposure, and basal cell hyperplasia and the development of squamous epithelium after 8 and 10 days.