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소모성 질환에 이환된 이유자돈에 대해 Porcine cytomegalovirus DNA Polymerase 유전자 검출과 분석
김지언,김지영,楚佳奇,채미경,장경수,김명철,박창식,전무형 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-
Porcine cytomegalovirus (PCMV) causes piglet deaths, runting, inclusion body rhinitis, respiratory and reproductive disorders in swine herd, resulting in the significant economic losses in the swine industry. Recently, in the field of pig-to-human xenotransplantation, PCMV has been indicated as a potential xenogeneic pathogen with the risk for infection transmitted from the xenograft donor to the recipient. In this study, PCMV DNA polymerase gene was detected in the sera and nasal swabs from 30 heads of the weaned piglets affected with various wasting diseases and 10 heads of normal healthy piglets using polymerase chain reaction (PCR), and the diagnostic efficacy of PCR was evaluated. The prevalence of the PCR-positive by specimens from the diseased piglets were 83.3% in sera and 33.3% in nasal swabs. In the normal healthy group, the PCR-positive rates of sera and nasal swabs were 40% and 30%, respectively. Ten piglets (25.0%) out of 40 showed the simultaneously positive results in both of sera and nasal swabs. According to this result, it was found that the serum specimen was the most appropriate sample for detection of PCMV by PCR. The nucleotide sequence homology of DNA polymerase gene of PCMV KS1 as compared with the reference strain of PCMV (GenBank accesson no.AF027217) was 99.0%.
FIBRIN GLUE가 이식 피부의 접착 및 생착에 미치는 효과
백무현,홍종현,박언섭,민대홍,김승홍,김미경 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.2
The ability of a skin graft to adhere initially to a wound a bed is dependent on the formation of a stable fibrin bond the elastin in the graft and the elastin in the bed. the Factors preventiong such adhesion most commonly include bleeding, infection, mechanical disturbances of the adhesive mechanism, i.e., motion. This study was conducted to evaluate the adhesive effect of fibrin glue in the full-thickness skin grafts at 3,5,30,120minute and 8 hours after gluing in mice and the effect of fibrin glue on the skin graft take at the 3,7,15post operative days in rabbits. The result were as follows; (1) Mechanical testing demostrated that the shear adhesive strength of non-fibrin gluing control group was found to be 2.84m²(SD=2.68) after 3minutes of fixation At, 5minutes, the adhesive strength was 9.78gm/㎠(SD=2.68) after 3minutes of fixation. At 5minutes, the adhesive strength was 9.78gm/㎠(SD=5.61);at 30minutes, 30.78gm/㎠(SD=5.42); at 2hours, 53.14gm/㎠(SD=11.50); and at 8 hours, 108.08gm/㎠(SD=15.78) and that the shear adhesive strength of fibrin glue applied group was found to be 17.50gm/㎠(SD=5.79) after 3minutes of of fixation(after gluing). AT 5minutes, the adhesive strength was 24.30gm/㎠(SD=3.57); at 30minutes, 105.48gm/㎠(SD=15.27); at 2hours, 167.54gm/㎠(SD=58.12); and at 8hours, 286.22gm/㎠(SD=28.27). These results in shear adhesive strength were a significantly different between the experimental and the control groups at early skin grafts adherence. (2) The histologic examination did not demonstrate significant difference in healing process when the fibrin glue applied group were compared with the control group. With the above results, fibrin glue improved in early graft adherence without interference of the take of graft.
MgCl₂溶液內에서 STS 304 HP Stainless Steel 熔接部의 破壞擧動에 對한 硏究
金永奭,丁太權,朴昌彦,洪錫柱,梁仁榮,金基玉,朴煥奎,李茂錫,曺圭宰,鄭在康 조선대학교 생산기술연구소 1983 生産技術硏究 Vol.1983 No.-
This paper is based on an experiment analysing the stress corrosion cracking behaviour of welding specimens compared to that of non-welding specimens of STS 304 HP stainless steel in corrosion solution. As a corrosion condition, stainless steel has been known to be the most fragile in a solution of 42% MgCl₂heated to the boiling point, therefore the experiment was carried out in this condition. The experiment was carried out with argon gas TIG welding specimens and non-welding ones made of stainless steel plates, thickness 2mm, by tensile force. At this time, the tensile force was taken to be the value of the yield strength of the tensile test specimens divided by the safety factor of 3.0 to 3.5, 4.0, 4.5, and 5.0. In this paper, the relations between Time of Initial Crack, Time of Failure, Time of Final Fracture and Crack Propagation Length were investigated and then plotted. Furthermore, the relations between Stress Intensity Factor K proposed by Irwin and the Time of Initial Crack, the Time of Failure, the Time of Final Fracture were calculated and plotted. At the last, all the relations plotted were expressed to normal equations by computer, and the equations were used to analyse the data obtained in the experiment. The conclusions obtained in this study are as follows: 1. Welding materials, compared with non-welding ones, as the Time of Initial Crack, the Time of Failure, and the Time of Final Fracture were generally very fast, appeared to be a phenomenon of unstable brittleness fracture. 2. Non-welding materials, compared with welding ones, as the safety factor was larger or the working stress was smaller, appeared to be a phenomenon that the longer had had greater durability. Therefor, welding materials must be considered enough to be used in heate affected and stress corrosion atmosphere. 3. The normal equations of the Time of Initial Crack and the variation rate to the same Stress Intensity Factor K are as follows: (A) Non-welding T =259702.4-5451.2 K+28.386 K² T = -5451.2+28.386 K (B) Welding T= -332.693+28.043 K-0.211 K² T' =28.043-0.211 K here, T; Time of Initial Crack, T'; Variation Rate of Time of Initial Crack to Stress Intensity Factor K, K ; Stress Intensity Factor.
농업투자용 시범농장 조성 후보지 선정을 위한 라오스 우돔싸이주(州)의 토양조사 및 토지특성 평가
박무언(Moo-Eon Park),박기욱(Ki-Wook Park),조일환(Il-Hwan Cho) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.6
라오스 우돔싸이주에 대한 농업투자를 촉진하기 위하여 농어촌공사의 지원을 받아 성덕농산(합)이 실시한 농업투자환경조사의 일환으로 시범농장 후보지 14개소에 대한 토양의 형태적 특성 조사와 급수평가법에 의한 적합정도 평가 및 시범농장 예정지에 대한 토양이화학성을 분석하였다. 라오스 우돔싸이주는 FAO 토양분류체계로 Leptosols, Fluvisols, Arenosols, Regosols, Alisols,Acrisols, Luvisols, Lixisols, Cambisols 등 9개 토양이 분포하고 그 중 알직층 (argic horizon)을 가진 Alisols,Acrisols, Luvisols, Lixisols 등 4개 토양이 우돔싸이주 총면적 1,537천 ha의 77%를 차지하고 있다. 우돔싸이주에서 14개 지점의 토양에 대한 형태적 특성을 조사하고 급수평가법으로 시범농장 적합성을 평가한 결과 6개 지점의 토양이 농장개설에 가장 무난한 것으로 평가되었다. 이들 6개 지점중 우돔싸이주 정부에서 가장 강력하게 추천하고 있는 1개소에 대하여 논과 밭에서 표토 (0~15 cm)와 심토 (20~40 cm)로 구분?채취하여 이화학성을 분석하였다. 시범농장 예정지의 토양은 점토함량이 44 %이상인 식질 토양으로 점착성과 가소성이 매우 높았고, 수리전도도는 논토양이 평균 26.27 cm day<SUP>-1</SUP>이고 밭토양이 40.64 cm day<SUP>-1</SUP>였다. 시범농장 예정지의 논토양은 약산성인데 반하여 밭토양은 강산성을 나타냈고, 토양유기물함량은 논토양이나 밭토양 공히 14 g kg<SUP>-1</SUP>이하, 유효인산 함량은 3 mg kg<SUP>-1</SUP>이하로 매우 낮은 수준이었지만 교환성 양이온은 각각 K 0.38, Ca 11, Mg 3.1 cmolc kg<SUP>-1</SUP>이하로 분석되어 비교적 적은 편은 아니었다. 따라서 라오스 우돔싸이주에서 농장조성시 봉착될 가장 중요한 장애요소는 점토함량이 많은 중점질 토양, 높은 토양침식성, 매우 낮은 유기물과 인산함량, 관개용수의 부족, 가파른 경사 등으로 요약된다. In order to select the demonstration farm site for agricultural investment by Korean fund, 14 sites were investigated by soil morphological characteristics and were evaluated by rating method in the Oudomxai province of Laos. Land evaluation was carried out by using eight factors, such as site accessibility, soil erosion susceptibility, easiness of farm mechanization, irrigation water obtainability, suitability of soil physical and chemical properties for crop growth, cost for establishment of farm foundation and land obtainability. In addition, one site to have been highly ranked was soil physico-chemically studied for farm planning. The site of heavy clayey soil has hydraulic conductivity of 26.27~40.64 cm day-1, organic content of lower than 14 g kg-1, available phosphate content of lower than 3 mg kg-1, exchangeable cations of lower than 0.38, 11 and 3.1 cmolc kg-1 in K, Ca and Mg, respectively. Major important limitations for establishment of demonstration farm were concluded as heavy soil-texture, high soil erodibility, low organic matter and phosphate contents, and insufficient irrigation water in the Oudomxai province of Laos.
Polyamine Group Assembled Silica Coated Ferrite Nanoparticle for Lambda DNA Detection
Park Moo-Eon,Chang Jeong-Ho 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1
The magnetic ferrite nanoparticles were synthesized and coated by silica precursor in controlling the coating thicknesses and sizeses. The surface modification was performed with amino-functionalized organic silanes on silica coated magnetic nanoparticles. The use of functionalized self-assembled magnetic ferrite nanoparticles for nucleic acid separation process give a lot of advantages rather than the conventional silica based process.