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A New Formulated Stable Papin-pectin Aerosol Spray for Skin Wound Healing
Karla Monzerratt Gregorio Jáuregui,Juan Carlos Cano Cabrera,Elda Patricia Segura Ceniceros,Jose Luis Martínez Hernández,Anna Ilyina 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4
A new spray-on topical wound debrider composition consisting of 0.1% w/v papain immobilized in 6% w/v pectin gel was formulated for skin wound healing. The stability of the enzyme activity of this new formulated spray was compared with the 0.1% w/v papain in water solution at the refrigerated temperature of 4 and 75°C. Two aerosol propellants, air and nitrogen, were evaluated for their efficacy. The new formulated spray was then evaluated for its thermal stability under air and N2 at 75°C. All enzyme activity of papain was measured with a spectrophotometer using casein as a substrate at 37°C and in 0.1 M phosphate buffer at pH 6, as well as trichloral acetic acid to separate the product of casein hydrolysis. All the evaluations showed the new aerosol spray formulation to be far better than the liquid formulation in storage stability and thermal stability at 75°C under atmospheric or higher pressures. The new formulation was tested in a rabbit experiment to evaluate the effectiveness of wound healing. As compared to untreated wound, the formulated spray clearly was superior, yielding a 20% more healing progress in the first 4 days alone.
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