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Lainez, Marielena Moncada,Hsia, Liang Chou Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.10
The objective of the paper was to study the drinking and other related behavior of dairy cows (Bos taurus). There were 142 Holstein dairy cows observed and compared in this study. The experiment was designed on the basis of two different housing systems (wet pad with forced ventilation cooling house and open house); two different seasons (winter and summer); four different stages (high milk yielding cows, low milk yielding cows, dry cows, and heifers); and grouping (home and visitor animals). All cows had free access to water. Dairy cows spent 13.8 min/day drinking in wet-pad house and 11.7 min/day in open house. owever, there was no significant difference in the duration of water drinking between these two housing systems (p>0.05). The water consumption was significantly higher in wet-pad housed animals (68 L/day) than open-housed animals (31.5 L/day) (p<0.05). A significant interaction between housing and grouping (p<0.05) was found. Home and visitor animals spent more time drinking in open house, wet-pad house, respectively. A highly significant interaction was found between housing and drinking time during the day (p<0.001). Animals in open house drank more during the morning (6:00 to 10:00 h), whereas wet-pad housed animals drank in the afternoon (14:00 to 15:00 h) and evening (18:00 to 20:00 h). The average time a cow spent in drinking in summer was not ignificantly different from that of drinking in winter. However, the water intake was significantly higher in summer (61.9 L/day) than in winter (38.6 L/day) (p<0.05). Drinking activity showed a highly significant interaction between season and physiological stage (p<0.01). High milk yield cows spent more time drinking in summer than in winter, whereas cows in all other stages followed the opposite drinking pattern. Grouping exchange did not influence the drinking behavior of dairy cows in either season (p>0.05); both home and visitor animals spent almost the same time in drinking water. A strong significant interaction between season and time during the day was found(p<0.01), suggesting that animal's high drinking frequency occurred during the daytime for both seasons, with a peak midday in winter and two peaks at 10:00 h in the morning and 19:00 h in summer. Thus, drinking behavior was associated with the cooler time of day in summer and with the warmer hours of day in winter. High and low milk yielding cows and heifers spent 15.3 min/day, 14.3 min/day, and 12.8 min/day, respectively, in water drinking activity, but there was no significant difference among them (p>0.05). There was, however, a significant difference in water drinking activity found in dry cows, which spent less time in drinking at 8.2 min/day (p<0.05).
Consumption of an "Energy Drink" does not Improve Aerobic Performance in Male Athletes
( Monica Umana Alvarado ),( Jose Moncada Jimenez ) 한국스포츠정책과학원(구 한국스포츠개발원) 2005 International Journal of Applied Sports Sciences Vol.17 No.2
Energy drinks differ from sport drinks in their composition and purpose; sports drinks are consumed primarily to replenish carbohydrate and electrolytes loss during exercise, while energy drinks are used to stimulate the central nervous system in activities different than sports. The main purpose of the study was to determine if the intake of a commercially available energy drink enhanced motor performance in male athletes. A double-blind, randomized-crossover study was carried on 11 athletes who completed two 10 km cross-country races. Subjects drank 6 ml·kg-1 body mass of an energetic drink or a placebo beverage 30 min before the race. Statistical analysis included analysis of variance, repeated measures t-student, and McNemar χ2 tests on the dependent variables, urine specific gravity, ratings of perceived exertion, heart rate, gastrointestinal symptoms, and performance time. The results indicated that no significant differences were found between mean racing times; however, ratings of perceived exertion were significantly lower when participants ingested the energy drink (energy drink= 7.02 ± 1.21 vs. placebo = 8.01 ± 0.75) (p < .05). No significant associations were found between the energy drink or the placebo and gastrointestinal symptoms. Finally, no specific personality type was related to the placebo effect. In conclusion, even though athletes did not improve their racing times when consuming the energy drink, their perceived exertion was lower during the running trials.
The Third Version of the Copenhagen Psychosocial Questionnaire
Burr, Hermann,Berthelsen, Hanne,Moncada, Salvador,Nubling, Matthias,Dupret, Emilie,Demiral, Yucel,Oudyk, John,Kristensen, Tage S.,Llorens, Clara,Navarro, Albert,Lincke, Hans-Joachim,Bocerean, Christin Occupational Safety and Health Research Institute 2019 Safety and health at work Vol.10 No.4
Introduction: A new third version of the Copenhagen Psychosocial Questionnaire (COPSOQ III) has been developed in response to trends in working life, theoretical concepts, and international experience. A key component of the COPSOQ III is a defined set of mandatory core items to be included in national short, middle, and long versions of the questionnaire. The aim of the present article is to present and test the reliability of the new international middle version of the COPSOQ III. Methods: The questionnaire was tested among 23,361 employees during 2016-2017 in Canada, Spain, France, Germany, Sweden, and Turkey. A total of 26 dimensions (measured through scales or single items) of the middle version and two from the long version were tested. Psychometric properties of the dimensions were assessed regarding reliability (Cronbach α), ceiling and floor effects (fractions with extreme answers), and distinctiveness (correlations with other dimensions). Results: Most international middle dimensions had satisfactory reliability in most countries, though some ceiling and floor effects were present. Dimensions with missing values were rare. Most dimensions had low to medium intercorrelations. Conclusions: The COPSOQ III offers reliable and distinct measures of a wide range of psychosocial dimensions of modern working life in different countries; although a few measures could be improved. Future testing should focus on validation of the COPSOQ items and dimensions using both qualitative and quantitative approaches. Such investigations would enhance the basis for recommendations using the COPSOQ III.
( Oscar Gutierrez Sancho ),( Jose Moncada Jimenez ),( Walter Salazar Rojas ),( Ellyn Robinson ) 한국스포츠정책과학원(구 한국스포츠개발원) 2006 International Journal of Applied Sports Sciences Vol.18 No.2
Creatine supplementation has been widely used for athletes and in some studies it has been proved effective; however, there remain some moderator variables that still require meta-analysis. Therefore, the purpose of this study was to conduct such an analysis on the effects of creatine supplementation on biochemical, body composition, and physical performance variables. From a total of 120 experimental studies found in databases, only 55 studies (46 %) were considered appropriate and/or contained the necessary information to obtain effect sizes. Creatine supplementation produced biochemical changes, including increases in excretion parameters. Anthropometric variables, body fat percentage, and lean mass were favorably changed by creatine supplementation. Improved peak power, total work, force power, and a maximal repetition (1RM) were also found following creatine supplementation. There was an evident placebo effect in the variables meta-analyzed; however, there was a clear increase in total work beyond the placebo effect. In summary, creatine supplementation consistently showed positive biochemical, body composition, and power changes in humans.
The Third Version of the Copenhagen Psychosocial Questionnaire
Hermann Burr,Hanne Berthelsen,Salvador Moncada,Matthias Nübling,Emilie Dupret,Yucel Demiral,John Oudyk,Tage S. Kristensen,Clara Llorens,Albert Navarro,Hans-Joachim Lincke,Christine Bocéréan,Ceyda Saha 한국산업안전보건공단 산업안전보건연구원 2019 Safety and health at work Vol.10 No.4
Introduction: A new third version of the Copenhagen Psychosocial Questionnaire (COPSOQ III) has been developed in response to trends in working life, theoretical concepts, and international experience. A key component of the COPSOQ III is a defined set of mandatory core items to be included in national short, middle, and long versions of the questionnaire. The aim of the present article is to present and test the reliability of the new international middle version of the COPSOQ III. Methods: The questionnaire was tested among 23,361 employees during 2016e2017 in Canada, Spain, France, Germany, Sweden, and Turkey. A total of 26 dimensions (measured through scales or single items) of the middle version and two from the long version were tested. Psychometric properties of the dimensions were assessed regarding reliability (Cronbach a), ceiling and floor effects (fractions with extreme answers), and distinctiveness (correlations with other dimensions). Results: Most international middle dimensions had satisfactory reliability in most countries, though some ceiling and floor effects were present. Dimensions with missing values were rare. Most dimensions had low to medium intercorrelations. Conclusions: The COPSOQ III offers reliable and distinct measures of a wide range of psychosocial dimensions of modern working life in different countries; although a few measures could be improved. Future testing should focus on validation of the COPSOQ items and dimensions using both qualitative and quantitative approaches. Such investigations would enhance the basis for recommendations using the COPSOQ III.
( Gerardo A. Araya Vargas ),( Sherry Gapper Morrow ),( Jose Moncada Jimenez ),( Janet Buckworth ) 한국스포츠정책과학원(구 한국스포츠개발원) 2009 International Journal of Applied Sports Sciences Vol.21 No.1
The purposes of the study were to (1) translate the Mindful Awareness Attention Scale (MAAS) (Brown & Ryan, 2003) into Spanish and (2) make adjustments for its application to research in exercise psychology, sport and health. For the translation and transcultural validation, the Spanish version (T4) of the MAAS scale was reviewed by 100 advanced students and professors of Physical Education of the Universidad de Costa Rica. Next, T4 and the original English MAAS were completed by 58 students of the Master`s program in Translation of the Universidad Nacional (Costa Rica). The scales were randomly applied at the beginning and at the end of a three-hour class. Internal consistency measured by Cronbach`s alpha coefficient was adequate (a=0.88 T4 and α=0.84 original). Scores from the two versions were significantly correlated (r=0.94; p<0.01) and were not different (t=0.73; p>0.05). Thus it was found that the Spanish version of the MAAS is sufficiently valid and reliable to be implemented in research in exercise psychology, sports and health. Further studies must test construct validity of the Spanish version of the MAAS.
Edwin David Morales-Álvarez,Claudia Marcela Rivera-Hoyos,Angélica María Baena-Moncada,Patricia Landázuri,Raúl A. Poutou-Piñales,Homero Sáenz-Suárez,Luis A. Barrera,Olga Y. Echeverri-Peña 한국미생물학회 2013 The journal of microbiology Vol.51 No.2
The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78.13 to 94.35 ng/ml and the specific activity varied from 34.20 to 25.97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold,compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like)emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization,future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli.