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      • Effects of Different Electron Donors on Anode Bacterial Communities and Microbial Fuel Cells Performance

        Mohammod MAHASIN ALI,Sokhee JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

        Microbial fuel cells (MFCs) harness the electrochemical activity of certain microbes for the production of electricity from reduced compounds. Characterizations of MFC anode biofilms have collectively shown very diverse microbial communities, raising ecological questions about competition and community succession within these anode-reducing communities. Three sets of triplicate, two-chamber MFCs inoculated with anaerobic sludge and differing in energy sources (acetate, lactate, and glucose) were operated to explore these questions. Based on 16S rDNA-targeted denaturing gradient gel electrophoresis (DGGE), all anode communities contained sequences closely affiliated with Geobacter sulfurreducens (>99% similarity) and an uncultured bacterium clone in the Bacteroidetes class (99% similarity). Various other Geobacter-like sequences were also enriched in most of the anode biofilms. While the anode communities in replicate reactors for each substrate generally converged to a reproducible community, there were some variations in the relative distribution of these putative anode-reducing Geobacter-like strains. Firmicutes were found only in glucose-fed MFCs, presumably serving the roles of converting complex carbon into simple molecules and scavenging oxygen. The maximum current density in these systems was negatively correlated with internal resistance variations among replicate reactors and, likely, was only minimally affected by anode community differences in these two-chamber MFCs with high internal resistance.

      • KCI등재

        Development of a Plum(Japanese Apricot) Seed Crusher to Control Harmful Larvae(Eurytoma maslovskii) in Plum Orchard

        Mohammod Ali,Bok Seok Kim,Seong-Jin Park,김혁주 경상국립대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.2

        The Eurytoma maslovskii conduces the unexpected plum dropping. It stayed overwinter insidethe pits of the dropped plums and able to oviposit on plum surface when the diameter of theplums are over 16 mm prior to endocarp hardening. To control this harmful pests is too muchessential to plum orchard. Therefore, the plum seed crusher (PSC) was designed and developedaccording to the purpose of perishing the pests through destroying the dropped plums. The rocksand the weeds were removed from the plum orchard prior to the test. Manually operated plumseed crusher picked-up the dropped plums from the ground and crushed them. The performanceof PSC was evaluated by applying 2.5 and 3.0 km/h travelling speeds, and controlling the gaps;18, and 28 mm intervals between plum pick-up discs. The test result showed the plum pick-uprate was 98±2.0%, and 92±5.3% with the machine travelling speed of 3.0 km/h in laboratory. Further, it changed to 95±8.7%, and 93±5.8% when the travelling speed was used 2.5 km/h. Inboth cases of test plum pick-up discs were maintained 18 mm, and 28 mm gap intervals,respectively. Furthermore, in field conditions, pursuing to similar gap intervals, the plum pick-uprate was 94±4.0%, and 92±2.0% with the speed of 3.0 km/h albeit it showed 92±4.0%, and90±3.5% when the speed was decreased to 2.5 km/h. The maximum plum pick-up rate wasfound at 18 mm disc intervals with 3.0 km/h travelling speeds. The crushing teeth were selected8 mm in height by repeated test to destroy the plum seeds. The result showed 100% crushingefficiency was facilitated to entire tests at 280 RPM crushing speeds regardless of plum sizesand shapes. This machine is preferred for the farmers to control the damage of plums bypreventing the propagation of the identified pests.

      • Effects of Medium pH Changes on the Electrochemical Characteristics Behavior of a Microbial Fuel Cell

        Mohammod MAHASIN ALI,Sokhee JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

        pH oppositely influences anode and cathode performance in microbial fuel cells. The differential electrochemical effects at each electrode and the resultant full-cell performance were analyzed in medium pH from 6.0 to 8.0.Potentials changed -60 ㎷/pH for the anode and -68 mV/pH for the cathode, coincident with thermodynamic estimations. Open circuit voltage reached a maximum (741 ㎷) at pH 7, and maximum power density was highest(712 ㎷/m2) at pH 6.5 as the cathode performance improved at lower pH. Maximum current density increased and apparent half-saturation potential (EKA) decreased with increasing medium pH due to improved anode performance. An equivalent circuit model composed of two time constant processes accurately fit bioanode impedance data. One of these processes was consistently the rate-limiting step for acetate-oxidizing exoelectrogenesis, with its pH-varying charge transfer resistance R2 ranging from 2- to 321-fold higher than thepH-independent charge transfer resistance R1. The associated capacitance C2 was 2-3 orders of magnitude larger than C1. R2 was lowest near EKA and increased by several orders of magnitude at anode potentials above EKA,while R1 was nearly stable. However, fits deviated slightly at potentials above EKA due to emerging impedance possibly associated with diffusion and excessive potential.

      • Development of a Plum Seed Remover for Multipurpose Plum Flesh Processing

        ( Mohammod Ali ),( Seong-jin Park ),( Seong-yoon Cho ),( Jong-ho Lee ),( Tangina Akhter ),( Kyu-won Yang ),( Gwang-shim Kim ),( Hyuck-joo Kim ) 한국농업기계학회 2017 한국농업기계학회 학술발표논문집 Vol.22 No.2

        Plum (Prunus mume) has various medicinal and economical applications. Nowadays, the plums are very popular in South Korea as well as over the world, but extracting of plum seed remain a serious impediment in their processing. Therefore, we developed a plum seed remover (PSR) with capacity of 80 kg · h<sup>-1</sup> which can use various types of cutters according to the purpose of plum processing and evaluated its performances. At first, the PSR in which multipurpose cutters- zero, two, and four-blade cutters can be installed was designed and manufactured. For identifying appropriate parameters related to cutting pressure, plums were harvested from three regions with three harvesting periods and their geometrical and mechanical properties were measured. After the application of the parameters related to cutting pressure, the performance test was carried out to both fresh and frozen plums by identifying the ratios of flesh recovery, seed recovery, seed breakage, deseeding efficiency, and machine efficiency. Evaluation was done by conducting experiment set different angle of positions (0°, 15°, 45°, 90° and 180°). The testing results of the PSR showed that 100% deseeding efficiency was facilitated regardless of the types of cutters. However, in case of four-blade cutter, there are significant differences in flesh recovery ratio according to the plum setting angle. The developed PSR was recorded about 80% average flesh recovery for fresh plum and about 70% flesh recovery for frozen plum. As a result, between fresh and frozen plums, all cutters showed significantly better flesh recovery ratio for the case of fresh plums. As compared to traditional plum seed separator developed PSR reducing manual labor and saving operating time and increasing machine productivity. Finally, this machine will advance the plum processing technology, and eventually flourish the plum industry.

      • KCI등재후보

        Development of a sandy soil water content monitoring system for greenhouses using Internet of Things

        Mohammod Ali,Md Razob Ali,Md Ashrafuzzaman Gulandaz,Md Asrakul Haque,Md Sazzadul Kabir,Sun-Ok Chung 사단법인 한국정밀농업학회 2023 정밀농업과학기술지 Vol.5 No.3

        Precision water management is crucial for greenhouse agriculture to maximize crop yields in sandy soil. Due to the low water holding capacity, it is necessary to monitor the water movement in different depths of sandy soil to ensure effective irrigation. Therefore, this study aimed to develop a data acquisition (DAQ) system for sandy soil water content monitoring in an experimental soil bin inside a greenhouse, utilizing the capabilities of the Internet of Things (IoT). A drip irrigation system was implemented, arranged in four pipelines, spaced 60 cm apart, with drippers placed at 30 cm intervals along the pipeline. The overall system was installed in a sandy soil testing bin. A DAQ system was comprised of three basic units: sensor interfacing and circuit board, programming and sensor data acquisition, and data storage and monitoring. A microprocessor was used by interfacing a set of soil water content sensors, ambient temperature, and humidity sensors. The water content sensors were placed in the soil at different depths of 10, 20, 30, 40, and 50 cm, respectively. A microcontroller was used to collect and send the sensor data to monitor and store in memory. During the test, the maximum and minimum average of soil water content, ambient temperature, and humidity values were observed at 33.91±2.5 to 26.95±1.3%, 21.39±2.1 to 42.84±1.7°C, and 48.73±2.3 to 99.90±0.3%, respectively. The water content percentages were varied at different depths of sandy soil due to low water holding capacity. The developed automatic DAQ system would help with remote monitoring and control of greenhouse irrigation, considering the different crop characteristics and environmental conditions.

      • Bacterial Communities of Microbial Fuel Cells in Responses to the External Resistance

        Mohammod MAHASIN ALI,Sokhee JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

        The external resistance (R ext) of microbial fuel cells (MFCs) regulates both the anode availability as an electron acceptor and the electron flux through the circuit. We evaluated the effects of R ext on MFCs using acetate or glucose. The average current densities (I) ranged from 40.5 ㎃/m2 (9,800 Ω) to 284.5 ㎃/m2 (150 Ω) for acetatefed MFCs (acetate-fed reactors [ARs]), with a corresponding anode potential (E an) range of -188 to -4 ㎷ (versus a standard hydrogen electrode [SHE]). For glucose-fed MFCs (glucose-fed reactors [GRs]), I ranged from 40.0 ㎃/m2 (9,800 Ω) to 273.0 ㎃/㎡ (150 Ω), with a corresponding E an range of -189 to -7 ㎷. ARs produced higher Coulombic efficiencies and energy efficiencies than GRs over all tested R ext levels because of electron and potential losses from glucose fermentation. Biogas production accounted for 14 to 18% of electron flux in GRs but only 0 to 6% of that in ARs. GRs produced similar levels of methane, regardless of the R ext. However, total methane production in ARs increased as R ext increased, suggesting that E an might influence the competition for substrates between exoelectrogens and methanogens in ARs. An increase of R ext to 9,800 Ω significantly changed the anode bacterial communities for both ARs and GRs, while operating at 970 Ω and 150 Ω had little effect. Deltaproteobacteria and Bacteroidetes were the major groups found in anode communities in ARs and GRs. Betaproteobacteria and Gammaproteobacteria were found only in ARs. Bacilli were abundant only in GRs. The anode-methanogenic communities were dominated by Methanosaetaceae, with significantly lower numbers of Methanomicrobiales. These results show that R ext affects not only the E an and current generation but also the anode biofilm community and methanogenesis.

      • Design, Development and Evaluation of Automatic Plum Seed Remover

        ( Mohammod Ali ),( Seong-jin Park ),( Tangina Akhter ),( Gwang-shim Kim ),( Kyu Won Yang ),( Hoon Seonwoo ),( Hyuck-joo Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

        Plum (Japanese Apricot) is ordinarily familiar with as medicinal and economical fruits. Further processing of plum fleshes amplifies its extra charges. Serious obstacles of processing is the removing of it seeds. It is presently being deseeded in the absence of suitable mechanical devices in the Rep. of Korea. An identified and developed automatic plum seed remover could reduce the flesh loss apparently, and increase its importance. Therefore, an Automatic Plum Seed Remover (APSR), was designed, manufactured and its performance was tested. The introduced APSR, which comprised of an automatic plum supplying units, plum positioning units such design is characterized by a rotating taper-shape rollers that set the plums into desired angle of positions, main punching and cutting blades, commonly used various type of cutters, namely, zero, two and four blade cutters, those are boosted for multipurpose plum flesh processing. In addition, it was used under cutter blades in the purpose of reducing pressure, increasing outputs, and getting quality fleshes. The performance parameters of the APSR are assessed including the ratios of the flesh recovery, seed recovery, seed breakage, deseeding efficiency, and machine efficiency. The evaluations were done by conducting experiments at different conditions, based on positioning competency on both fresh and frozen plums. It exhibited the better outcomes comparing with other ones. The test results of the developed machine showed, 100% deseeding efficiency, which was facilitated for both ripe and hard plums with properly calculated pressure. The developed APSR efficiency was 80 kg/h, no seed breakage occurred while using its mechanism, in case of four-blade cutter, there were significant differences in flesh recovery ratios according to the plum setting angle nearly, 0°, 15° and 180°. The developed automatic plum seed remover was recorded about 87.90 %, average flesh recovery for ripe plums and about 83.02% flesh recovery for hard plums. As a result, between both of hard and ripe plums, cutters showed significantly better flesh recovery ratios for the cause of the ripe plums. These set of conditions are preferred, because of its minimal loss, rapid operation, increased productivity, correct plum positioning and effective under cutter options as well as low breakage factor, and its automatic systems also reduce the manual labor expenditure. Hence, this APSR will take in advance plum processing technology and help to flourish the plum industry.

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