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Regeneration of Plantlets from petiole callus of wild viola (Viola patrinii DC.)
Miyake,Hiroshi,Taniguchi,Takeshi,Sato,Tadahiko,Oh,Chang Kwon,Maeda,Eizo 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3
Plantlets were regenerated from 5-year subcultured compact callus derived from petiole tissues of wild viola (Viola patrinii DC.) but not from 5-year subcultured friable callus. Regeneration occurred most efficiently on medium that contained two-fold diluted basal salts of Murashige and Skoog's (MS) medium, 5×10-6M 1-naphthaleneacetic acid and ?? M kinetin. The effect of dilution of MS basal salts could also be achieved solely by two-fold dilution of the potassium dihydrogen phosphate in the mixture. The present study revealed that dilution of MS basal salts, in particular of potassium dihydrogen phosphate, was important for the regeneration of wild viola. Moreover, although the callus had been subcultured for 5 years, regeneration of plantlets from callus was still possible. In addition, scanning electron microscopy revealed that details of the process of plant regeneration from subcultured callus varied with the age and source of callus and differed from that reported in rice.
A SUB-SATURN MASS PLANET, MOA-2009-BLG-319Lb
Miyake, N.,Sumi, T.,Dong, Subo,Street, R.,Mancini, L.,Gould, A.,Bennett, D. P.,Tsapras, Y.,Yee, J. C.,Albrow, M. D.,Bond, I. A.,Fouqué,, P.,Browne, P.,Han, C.,Snodgrass, C.,Finet, F.,Furusawa, K IOP Publishing 2011 The Astrophysical journal Vol.728 No.2
<P>We report the gravitational microlensing discovery of a sub-Saturn mass planet, MOA-2009-BLG-319Lb, orbiting a K-or M-dwarf star in the inner Galactic disk or Galactic bulge. The high-cadence observations of the MOA-II survey discovered this microlensing event and enabled its identification as a high-magnification event approximately 24 hr prior to peak magnification. As a result, the planetary signal at the peak of this light curve was observed by 20 different telescopes, which is the largest number of telescopes to contribute to a planetary discovery to date. The microlensing model for this event indicates a planet-star mass ratio of q = (3.95 +/- 0.02) x 10(-4) and a separation of d = 0.97537 +/- 0.00007 in units of the Einstein radius. A Bayesian analysis based on the measured Einstein radius crossing time, t(E), and angular Einstein radius,theta(E), along with a standard Galactic model indicates a host star mass of M-L = 0.38(-0.18)(+0.34) M-circle dot and a planet mass of M-p = 50(-24)(+44)M(circle plus), which is half the mass of Saturn. This analysis also yields a planet-star three-dimensional separation of a = 2.4(-0.6)(+1.2) AU and a distance to the planetary system of D-L = 6.1(-1.2)(+1.1) kpc. This separation is similar to 2 times the distance of the snow line, a separation similar to most of the other planets discovered by microlensing.</P>
Miyake, N.,Tsukaguchi, H.,Koshimizu, E.,Shono, A.,Matsunaga, S.,Shiina, M.,Mimura, Y.,Imamura, S.,Hirose, T.,Okudela, K.,Nozu, K.,Akioka, Y.,Hattori, M.,Yoshikawa, N.,Kitamura, A.,Cheong, H.,Kagami, S University of Chicago Press [etc.] 2015 American journal of human genetics Vol.97 No.4
The nuclear pore complex (NPC) is a huge protein complex embedded in the nuclear envelope. It has central functions in nucleocytoplasmic transport, nuclear framework, and gene regulation. Nucleoporin 107 kDa (NUP107) is a component of the NPC central scaffold and is an essential protein in all eukaryotic cells. Here, we report on biallelic NUP107 mutations in nine affected individuals who are from five unrelated families and show early-onset steroid-resistant nephrotic syndrome (SRNS). These individuals have pathologically focal segmental glomerulosclerosis, a condition that leads to end-stage renal disease with high frequency. NUP107 is ubiquitously expressed, including in glomerular podocytes. Three of four NUP107 mutations detected in the affected individuals hamper NUP107 binding to NUP133 (nucleoporin 133 kDa) and NUP107 incorporation into NPCs in vitro. Zebrafish with nup107 knockdown generated by morpholino oligonucleotides displayed hypoplastic glomerulus structures and abnormal podocyte foot processes, thereby mimicking the pathological changes seen in the kidneys of the SRNS individuals with NUP107 mutations. Considering the unique properties of the podocyte (highly differentiated foot-process architecture and slit membrane and the inability to regenerate), we propose a ''podocyte-injury model'' as the pathomechanism for SRNS due to biallelic NUP107 mutations.
Miyake, N.,Udalski, A.,Sumi, T.,Bennett, D. P.,Dong, S.,Street, R. A.,Greenhill, J.,Bond, I. A.,Gould, A.,Kubiak, M.,Szymań,ski, M. K.,Pietrzyń,ski, G.,Soszyń,ski, I.,Ulaczyk, K.,Wyrzyk IOP Publishing 2012 The Astrophysical journal Vol.752 No.2
<P>We report the extremely high-magnification (A > 1000) binary microlensing event OGLE-2007-BLG-514. We obtained good coverage around the double peak structure in the light curve via follow-up observations from different observatories. The binary lens model that includes the effects of parallax (known orbital motion of the Earth) and orbital motion of the lens yields a binary lens mass ratio of q = 0.321 +/- 0.007 and a projected separation of s = 0.072 +/- 0.001 in units of the Einstein radius. The parallax parameters allow us to determine the lens distance D-L = 3.11 +/- 0.39 kpc and total mass M-L = 1.40 +/- 0.18 M-circle dot; this leads to the primary and secondary components having masses of M-1 = 1.06 +/- 0.13 M-circle dot and M-2 = 0.34 +/- 0.04 M-circle dot, respectively. The parallax model indicates that the binary lens system is likely constructed by the main-sequence stars. On the other hand, we used a Bayesian analysis to estimate probability distributions by the model that includes the effects of xallarap (possible orbital motion of the source around a companion) and parallax (q = 0.270 +/- 0.005, s = 0.083 +/- 0.001). The primary component of the binary lens is relatively massive, with M-1 = 0.9(-0.3)(+4.6) M-circle dot and it is at a distance of D-L = 2.6(-0.9)(+3.8) kpc. Given the secure mass ratio measurement, the companion mass is therefore M-2 = 0.2(-0.1)(+1.2) M-circle dot. The xallarap model implies that the primary lens is likely a stellar remnant, such as a white dwarf, a neutron star, or a black hole.</P>
Yoshitaka Miyake,Ryo Kohsaka 한국식품연구원 2020 Journal of Ethnic Foods Vol.7 No.-
While neoliberalism has promoted free trade, market rule, and productivist agriculture in the food production system, farmers and their unions in developed countries partially managed to shun the forces of neoliberalism. What are the underpinning strength and factors of such resilience? Discussions have concentrated at national policy and organizational level and tacit resistance at community and farm levels remain unexplored, inter alia from their historical embeddedness perspective. This study explores the way Japanese farmers frame their contemporary political situation with neoliberalism of the late 2000s with a grounded approach of face-to-face interview at a community level. The farmers resist it mobilizing Scott’s anthropological notion of “Weapons of the Weak” through story-telling instead of hoes in ancient time (Scott JC, Weapons of the weak: everyday forms of peasant resistance, 1985). In concrete, the farmers resisted a neoliberal policy of Hinmoku Ōdanteki Keiei Antei Taisaku, or the MultiProduct Management Stabilization Plan (MPP), in the early 2000s, which promoted larger scale farming to pursue the efficiency of scale merits. The policy was first introduced in 2005 as a concept and dominated the agricultural policy scenes from 2007 to 2009, and then its influence disappeared toward 2010. The rise and fall of the concept and policy provide us with rare opportunity to examine the historical embeddedness of the farmers and their resistance to such neoliberal globalization. We conducted interviews with the leaders of cooperative farms and stakeholders in Daisen City, Akita Prefecture, Japan; the study found that farmers’ framing of neoliberalism was ambivalent causing partial adoption and resistance. The leaders of the cooperative farms could form the cooperative farms but thought further development difficult because members intended to keep their farming independent. Close frame and discourse analysis revealed that farmers in Japan could express their frustration on neoliberal discourse and policy through multiple tactics of “Weapons of the Weak” by complaining to the leaders, miscalculating the figures, or claiming family-based food sovereignty that “we produce what goes into our family mouths.” Such tactics procrastinated the process and eventually stopped the policy. The incident demonstrates how farmers in the network can slow the progress of neoliberal discourse and policy implementation. From the analysis, the notion of “Weapons of the Weak” can be applied as a part of the combination with farmers’ historical embeddedness, symbolism of foods, framing, and electoral resistance.