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Mittag, Maria,Lee, Dong-Hee,Hastings, J. Woodland 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-
In the marine dinoflagellate Gonyaulax polyedra there are two proteins, luciferase and luciferin binding protein (LBP), that correlate unambiguously with its bioluminescence. Its circadian rhythm of luminescence involves the synthesis and destruction of these two proteins; synthesis occurs as a several hours' long pulse each cycle, but the cellular amounts and in vitro activities of the mRNAs for these two proteins remain constant, indicating that the proteins are translationally controlled. We undertook the cloning and sequencing of both genes in order to search for control sequences important in temporal regulation. Both genes lack introns, as well as the common polyadenylation signal. The LBP gene also does not contain a TATA box in its promoter region. the copy number of LBP is very high (1000 copies per cell); some of these copies seem to be slightly different, as indicated by the existence of a variant LBP cDNA. to understand the mechanism we wought proteins binding to the 5' and 3' untranslated regions(UTRs) of LBP mRNA. None have been found with the 5'UTR, but one protein binding specifically to the 3'UTR could be detected. Current data indicate that this protein may be a dimer, acting as a repressor of translation during the day phase.
MITTAG, MARIA,LEE, DONG-HEE,HASTINGS, WOODLAND 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-
The circadian-expressed luciferin-binding protein from the dinoflagellate Gonyaulax polyedra is regulated at the translational level. We detected a protein, apparently a dimer, that binds specifically to the 3' untranslated region of its mRNA. Its binding site was localized within a 22-nt region in the 3' untranslated region containing seven UG repeats. The binding activity of this protein cycles on a daily bsis, decreasing at the beginning of the night when synthesis of luciferinbinding protein starts and increasing at the end of the night when synthesis of luciferin-binding protein stops. This suggests that it functions as a clock-controlled repressor, preventing the translation of lbp mRNA during the day.
Blended Learning in Practice: An Overview on Recent Developments
Hans-Joachim Mittag 한국방송통신대학교 미래원격교육연구원 2016 평생학습사회 Vol.12 No.2
The digitalization of education is a challenge for institutions involved in teaching and professional training. This paper summarizes recent developments and trends in this field. It deals with the ongoing evolvement of massive online courses (MOOCs) and the employment of mobile devices for learning and teaching purposes (m-learning). Furthermore, it describes well-established and new components of blended learning models applied in education and further education.
Lee, Dong-Hee,Mittag, Maria,Sczekan, Steve,Morse, David,Hastings, J.Woodland 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-
The circadian expressed luciferin-binding protein(LBP)gen from the marine bioluminescent alga Gonyaulax polyedra represents the first dinoflagellate gene that has been cloned and sequenced at both cDNA and genomic levels. Starting with a fragment from the 3'-end of the LBP eDNA that was found by immunoscreening of a cDNA library, genomic clones were obtained by the inverse polymerase chain reaction technique. Full-length cDNA clones were selected by sereening a cDNA library by plaque hybridizations and by polymerase chain reaction amplifications. The LBP sequence has a 2004-nucleotide open reading frame coding for a protein of 668 amino acids(~75kDa). The reading frame and identity of the clone were confirmed by the sequence of an octapeptide obtained from a purified fragment of CNBr-treated LBP. A variant LBP cDNA was found to differ in sequency by ~11% at the DNA level. The untranslated regions of the mRNA are 111 nucleotides (5'-untranslated region) and 158 nucleotides (3'-untranslated region) long, respectively. The LBP gene contains no introns and exhibits certain features not typical for a eukaryotic gene. Its promoter does not include the typical TATA box within ~50 nucleotides upstream of the transcription start site, and the usual poly (A+) signal (AAUAAA) is not present on the end of the LBP mRNA. The copy number of the gene is very high (~1000 copies/cell). However, the universal genetic code and coserved positions relevant for the translational apparatus are maintained.