IMAGE INTERACTIVITY TECHNOLOGY AND ENGAGEMENT ON ONLINE EXPERIENCE ON HOTEL WEBSITES: PERSONAL TRAITS’ MODERATING ROLE

Mirna M. Bassily 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Disposable Micropipette Tip for Purifying DNA Fragments

Mirna L. Sánchez,Claudia Gimenez,Leandro J. Martínez,M. Radrizzani,Mariano Grasselli 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2

A novel functional micropipette tip for DNA purification, called DNAtip, is described based on a novel coating process. The functional layer is prepared by an internal coating with silica particles supported into a polyvinyl alcohol (PVA) film. The coating layer is stabilized by simultaneous radiation-induced crosslinking to avoid its dissolution. Three types of silica particles of different diameters: micron, sub-micron, and nano sizes are studied. In addition, pH of the coating solution and concentration of the silica particles were optimized. Tips coated with fumed silica showed the highest absorption capacity of a plasmid DNA with an acceptable purity. Different linear dsDNA fragments were used for evaluating the absorption capacity and size discrimination of fragments. The recovery in the range of 700 ng and 200 ng per tip was measured for dsDNA fragments in the range of 400 bp to 2 kbp and 50 bp to 500 bp respectively. DNA PCR fragments contaminated with BSA were successfully purified with sufficient quality to be sequenced or enzymatically digested. This disposable tip device, useful for plasmids or DNA fragments purification, is a novel lab-on-a-tip device, which can effectively improve automate genetic engineering procedures

Wild Carrot Oil Extract is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells

Tawil, Mirna,Bekdash, Amira,Mroueh, Mohammad,Daher, Costantine F.,Abi-Habib, Ralph J. Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: In this study, we used Daucus carota oil extract (DCOE) to target acute myeloid leukemia (AML) cells. All the AML cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. Analysis of mechanism of cell death showed an increase in cells positive for annexinV and for active caspases, indicating that DCOE induces apoptotic cell death in AML. Inhibition of the MAPK pathway decreased sensitivity of AML cells to DCOE, indicating that cytotoxicity may be dependent on its activity. In conclusion, DCOE induces selective apoptosis in AML cells, possibly through a MAPK-dependent mechanism.

Analysis of Heterogeneity and Instability of Stable mAb-expressing CHO Cells

Zhimei Du,Mirna Mujacic,Kim Le,Guy Caspary,Heather Nunn,Carole Heath,Pranhitha Reddy 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

Screening and isolation of high expression mammalian cell lines for production of recombinant proteins for the clinic is a resource-intensive and time-consuming procedure due to the substantial variation in expression levels of recombinant protein expression amongst transfected cells. Several investigators have reported instability in expression titers early in cell line development and in cell banks. However, in most cases the exact molecular mechanisms of instability remain unknown. In this study we used a fluorescence-activated cell sorting (FACS) based mAb staining method to enable the detection and selective gating of cells with vastly different recombinant expression levels present in transfected pools. Expression diversity and changes within transfected populations were detected and isolated in real time during cell line development. Molecular genetic analysis on the isolated clones revealed an unsuspected rearrangement of the heavy chain in the non-expressing clones. Implications of the genetic rearrangements as well as the use of the FACS method as a tool to improve cell line development to detect expression heterogeneity in pools and to investigate root cause for the molecular genetics of expression instability will be discussed.

TRAIL and Bortezomib: Killing Cancer with Two Stones

Qureshi, Muhammad Zahid,Romero, Mirna Azalea,Attar, Rukset,Javed, Zeeshan,Farooqi, Ammad Ahmad Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Cancer genomics and proteomics have undergone considerable broadening in the past decades and increasingly it is being realized that solid/liquid phase microarrays and high-throughput resequencing have provided platforms to improve our existing knowledge of determinants of cancer development, progression and survival. Loss of apoptosis is a widely and deeply studied process and different approaches are being used to restore apoptosis in resistant cancer phenotype. Modulating the balance between pro-apoptotic and anti-apoptotic proteins is essential to induce apoptosis. It is becoming more understood that pharmacological inhibition of the proteasome might prove to be an effective option in improving TRAIL induced apoptosis in cancer cells. Keeping in view rapidly accumulating evidence of carcinogenesis, metastasis, resistance against wide ranging therapeutics and loss of apoptosis, better knowledge regarding tumor suppressors, oncogenes, pro-apoptotic and anti-apotptic proteins will be helpful in translating the findings from benchtop to bedside.

GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans

Nancy Dewi Yuliana,Mirna Zena Tuarita,Alfi Khatib,Farida Laila,Sukarno Sukarno 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.6

GC–MS metabolomics was used to discriminatethe phytochemicals profile of Indonesian white, red, andblack rice brans, and Japanese white rice brans. Thistechnique was used for the first time to identify compoundsin rice brans having cytotoxic activity against WiDr coloncancer cells. Orthogonal Projection to the Latent Structure(OPLS) analysis showed that protocatechuic acid (PA) wasa discriminating factor found in black rice brans whichstrongly correlated with its cytotoxicity (IC508.53 ± 0.26 lM). Real time-PCR data demonstrated thatPA cytotoxicity at different concentrations (1, 5, 10, 25 and50 lg/mL) was mediated through different pathways. Bcl-2 expression was downregulated at all tested concentrationsindicating apoptosis stimulation. At 1–10 ppm concentration,PA activated both intrinsic and extrinsicapoptosis pathways since the expression of p53, Bax,caspase-8, and caspase-9 were upregulated. At a higherdose (25 and 50 lg/mL), PA possibly involved in pyroptosis-mediated pro-inflammatory cell death by upregulatingthe expression of caspase-1 and caspase-7.

Evaluation of Management Performance for Heritage Buildings Case Study : Greco-Roman Museum – Alexandria, Egypt

Adel El-Menchawy,Wael Kamel,Amal Mamdouh,Mirna Eskander 대한건축학회 2023 Architectural research Vol.25 No.3

Building restoration is a complex process with a high level of uncertainty. Restoration professionals cansignificantly benefit from the well-established discipline of project management to achieve their targets; however,available evidence shows that the use of the project management body of knowledge in restoration projects is far fromthe desired level. Several historical organisations have since been established with the goal of preserving and governingcultural identity, and numerous studies have supported the need of preserving architectural heritage. Many owners,investors, academics, and developers believe that it would be considerably more expensive to renovate and restore anold building than to create a new one. Although the project management process is generally recognised, the conceptof project management for architectural heritage projects differs due to the uniqueness of each project. It differs frommany construction projects in terms of the need for research-based practices to define scope, planning, scheduling,supervision,decision-making,and also performance. The Greco-Roman Museum in Alexandria’s planning, design, andbuilding phases are being studied with the aim of identifying and analysing the variables that contribute to project delays. Three project management pillars were established as a result of gathering this data from the project’s stakeholders: thefirst pillar addresses time management for the existing phase and how it will be incorporated into the new extensionphase; the second pillar addresses performance in relation to project management issues in the delivery of the best qualityof a construction project; and the third pillar addresses the scope of the new extension because it will significantly impactthe other two pillars. This paper argues that a contemporary perspective which utilizes project management tools andtechniques can contribute to the conservation of architectural heritage in line with the conservation principles.

Hibiscus sabdariffa L.: phytoconstituents, nutritive, and pharmacological applications

Mohamed A. Salem,Ahmed Zayed,Magy E. Beshay,Mesih Mirna M. Abdel,Khayal Reem F. Ben,Fady A. George,Shahira M. Ezzat 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.3

Hibiscus sabdariffa L. (HS) calyx extracts have been well-documented for the treatment of hypertension, liver dysfunctions, and diabetes among others. An updated concise review of HS regarding phytoconstituents, and involved putative mechanisms of potential biological activities is presented. HS showed other food and other industrial applications, including the preparation of metallic nanoparticles. These activities were explained by the presence of a broad spectrum of valuable phytochemicals, including the red pigments anthocyanins, phenolic, and organic acids and polyphenolics (e.g., flavonoids and tannins). Most of the bioactivities were found to be associated with anthocyanins-rich extracts. Anthocyanins were found to have various mechanisms for the treatment of hypertension, including direct vasodilation and inhibition of angiotensinconverting enzyme (ACE). However, leaves and seeds showed also other potential applications in food industry, owing to the significant amounts of phenolic antioxidant compounds. Therefore, valorization and optimization of bioactive constituents’ extraction from plant biowastes should be considered for maximizing the benefits of HS extracts.

Recently Emerging Signaling Landscape of Ataxia-Telangiectasia Mutated (ATM) Kinase

Farooqi, Ammad Ahmad,Attar, Rukset,Arslan, Belkis Atasever,Romero, Mirna Azalea,ul Haq, Muhammad Fahim,Qadir, Muhammad Imran Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Research over the years has progressively and sequentially provided near complete resolution of regulators of the DNA repair pathways which are so important for cancer prevention. Ataxia-telangiectasia mutated kinase (ATM), a high-molecular-weight PI3K-family kinase has emerged as a master regulator of DNA damage signaling and extensive cross-talk between ATM and downstream proteins forms an interlaced signaling network. There is rapidly growing scientific evidence emphasizing newly emerging paradigms in ATM biology. In this review, we provide latest information regarding how oxidative stress induced activation of ATM can be utilized as a therapeutic target in different cancer cell lines and in xenografted mice. Moreover, crosstalk between autophagy and ATM is also discussed with focus on how autophagy inhibition induces apoptosis in cancer cells.

Screening and isolation of antibacterial proteinaceous compounds from flower tissues: Alternatives for treatment of healthcare-associated infections

Renato Goulart de Almeida,Simoni Campos Dias,Osmar Nascimento Silva,Elizabete de Souza Cândido,João Suender Moreira,Dianny Elizabeth Jimenez Jojoa,Diego Garcês Gomes,Mirna de Souza Freire,Pedro Henriq 셀메드 세포교정의약학회 2014 TANG Vol.4 No.1

Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.