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PIG3 Regulates p53 Stability by Suppressing Its MDM2-Mediated Ubiquitination
( Min Jin ),( Seon-joo Park ),( Seok Won Kim ),( Hye Rim Kim ),( Jin Won Hyun ),( Jung-hee Lee ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4
Under normal, non-stressed conditions, intracellular p53 is continually ubiquitinated by MDM2 and targeted for degradation. However, in response to severe genotoxic stress, p53 protein levels are markedly increased and apoptotic cell death is triggered. Inhibiting the ubiquitination of p53 under conditions where DNA damage has occurred is therefore crucial for preventing the development of cancer, because if cells with severely damaged genomes are not removed from the population, uncontrolled growth can result. However, questions remain about the cellular mechanisms underlying the regulation of p53 stability. In this study, we show that p53-inducible gene 3 (PIG3), which is a transcriptional target of p53, regulates p53 stability. Overexpression of PIG3 stabilized both endogenous and transfected wild-type p53, whereas a knockdown of PIG3 lead to a reduction in both endogenous and UV-induced p53 levels in p53-proficient human cancer cells. Using both in vivo and in vitro ubiquitination assays, we found that PIG3 suppressed both ubiquitination- and MDM2-dependent proteasomal degradation of p53. Notably, we demonstrate that PIG3 interacts directly with MDM2 and promoted MDM2 ubiquitination. Moreover, elimination of endogenous PIG3 in p53-proficient HCT116 cells decreased p53 phosphorylation in response to UV irradiation. These results suggest an important role for PIG3 in regulating intracellular p53 levels through the inhibition of p53 ubiquitination.
조민진 ( Jo¸ Min-jin ),김재엽 ( Kim¸ Jae-yeob ) 한국건축시공학회 2021 한국건축시공학회 학술발표대회 논문집 Vol.21 No.2
Based on changes in capacity demanded by society as a result of the 4th industrial revolution, as well as changes in the environment due to COVID19, it is necessary to adopt new educational methods in Korean universities. In response to such changes, there have been attempts to apply innovative teaching methods, such as flipped learning and blended learning. However, most studies are limited to either learning effects and/or satisfaction. Accordingly, this study seeks to contribute to the changing educational methods by developing online and offline blended building construction curriculum. The results of this study are as follows: combined education was made possible by developing a curriculum by dividing the online and offline environments. It is determined that the developed curriculum will be efficient and appropriate for the new environments.