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Mingtao Wu,Bing Guo,Qingliang Zhao,Jun Zhang,Xiaoyan Fang,Ping He 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.3
Accompanying with the extensive applications of micro-structured surfaces on hard and brittle material in MEMS and NEMS sensors, optical elements, electronic devices and medical products, efficiently fabricating of these surface has gradually become the focus of manufacturing community. Basing on precision grinding with conditioned and laser micro-structured coarse-grained diamond grinding wheel, a novel high efficiency technique for micro-structured surfaces on hard and brittle material, such as silicon carbide, was developed in this paper. Firstly, the maximum undeformed chip thickness for conditioned coarse-grained wheel and the ductile grinding of silicon carbide was theoretically and experimentally studied. Silicon carbide surface formed mainly in ductile regime was successfully achieved. And then, the strategy for micro-structuring the conditioned wheel with designed micro-structure geometry, sharp edge and small inclination angle side-wall was investigated. Finally, the linear and square micro-structured surfaces with high form accuracy and ultra-precision surface roughness were successfully and efficiently fabricated on silicon carbide by the technique developed in this paper.
Mingtao Zhu,Jun Yu,Min Zhao,Meijun Wang,Guoshun Yang 한국유전학회 2020 Genes & Genomics Vol.42 No.6
Background Grape is an economically valuable fruit around the world. However, some cultivars are prone to fruit cracking during ripening, leading to severe losses. Objective We aimed to find important metabolisms related to fruit cracking during ripening process. Methods RNA-Sequence and analysis was applied to the pericarp of cracking-susceptible ‘Xiang Fei’ at 1 (W1), 2 (W2) and 3 weeks (W3) after veraison on Illumina HiSeq xten; Results Compared with W1, the berry cracking rate increased significantly in W2 and W3. Through transcriptomic analysis, a total of 22,609 genes were expressed in the grape pericarp, among which 805 and 2758 genes were significantly differentially regulated in W1-vs.-W2 and W1-vs.-W3 comparison, respectively. Besides, 304 and 354 genes were up- and down-regulated in both comparisons. The significantly enriched GO terms of both W1–W2 and W1–W3 are related to cell wall and wax biosynthesis. And lipid metabolism, which are involved in the top 20 enriched KEGG pathways of both comparisons, was related to wax biosynthesis. Further, GO enrichment analysis of differentially expressed genes (DEGs) with same regulatory changes also indicated that the continuously up-regulated DEGs are significantly enriched in cell wall component biosynthesis and hydrolase. Conclusion These findings suggested that genes related to cell wall metabolism and cuticle biosynthesis may play important roles in regulating grape berry cracking. Our results provide a reference for further studies on the molecular mechanism underlying fruit cracking.
Chen Shunan,Li Mingtao,Liu Ji,Feng Ying,Yao Jun,Shi Lei,Chen Xiaoming 한국곤충학회 2021 Entomological Research Vol.51 No.10
Colors and chemicals are the most important cues for butterflies to localize mating partners and food. In this study, we used artificial flowers with different colors and plastic butterfly wing mimics to separate the impact of colors from other cues on foraging and courtship of the butterfly Papilio maackii. We found that males relied more on visual cues during foraging and courtship, but females responded more to olfactory signals. Males visited artificial flowers more frequently than females. However, when honey was applied on artificial flowers, the frequency of female’s flower visits increased and was much higher than that of male’s, suggesting that females may have stronger olfactory than visual senses. During courtship, females were passive, whereas males were very active in chasing females and driving away other males. Similar to courtship among butterflies in a natural population, males showed recognition ability to butterfly wing mimics, indicating that males could accurately identify mates using colors only without any chemical cues. We also analyzed volatiles and identified five types of male-specific chemicals while no female-specific chemicals were identified, suggesting that females might have used these male-specific volatiles to recognize and accepted males during courtship. Pollination of the plant Primula sieboldii mainly depends on P. maackii, and the plant has flowers with purple and mauve colors. Coincidently, P. maackii showed color preference to blue and purple, suggesting that the butterfly and plants might have coevolved during the long course of interactions.
Yanfeng Wang,Xudong Guo,Zhigang Wang,Zhixin Guo,Mingtao Liu,Keyu Zhao,Xue Feng,Xu Zheng,Xiaojing Wang,Huifang Hao 한국통합생물학회 2017 Animal cells and systems Vol.21 No.5
PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.