Applying the Principle of Maximum Entropy in Bayesian Prior Distribution Assignment

Song, Mingshun,Fang, Xinghua,Wang, Wei The Korean Society for Quality Management 2009 The Asian Journal on Quality Vol.10 No.3

Under the prior information that upper and lower bounds of the random quantity are symmetric with respect to the best estimate, this paper analyses the Bayesian prior distribution assignment using the principle of maximum entropy. With the exact lower and upper bounds, it approves uniform for the probability density function of the quantity and it has a curvilinear trapezoidal form for the inexact lower and upper bounds.

FOA Based Diagnosis Model for Multivariate Production Process

Yang Mingshun,Kong Xiangjian,Gao Xinqin,Liuyong,Li Yan 보안공학연구지원센터(IJUNESST) 2015 International Journal of u- and e- Service, Scienc Vol.8 No.1

Fault diagnosis for quality control during the multivariate production process is widely used to detect abnormal fluctuations, find out failure reasons and take measures to maintain the stability of the production system accordingly. The neural network methodology has become a main method in the field of intelligent diagnosis recently. However, it has certain deficiencies such as longer training time, slower convergence rate and easier falling into a local optimal solution easily. As a result, the effect of fault diagnose is influenced. Thus, this article proposes the idea of using the Fruit Fly Optimization Algorithm in the multivariable process fault diagnosis model, at the same time, to analyze the out of control sample data in the automobile crankshaft production. Compared with the neural network model in dealing with the fault diagnosis in multivariate process, Fruit Fly Optimization Algorithm’s effectiveness s verified.

善治视域下中国去南民族工作模式分析

杨文顺(Yang Mingshun),胡翠月(Hu Cuiyue) 동북아시아문화학회 2011 동북아 문화연구 Vol.1 No.27

As a province with the most residential, particular, cross border and autonomous ethnic groups, the largest number of autonomous minority nationality areas, as well as the second largest ethnic group population in China, ethnic work in Yunnan has typicality and representativeness. Under guidance of ethnic theories and policies with Chinese characteristics, ethnic work in Yunnan has explored a success mode fitting its own national and regional practicality. In substance, ethnic work in Yunnan realizes “good governance” in autonomous minority nationality area. In current situation, to summary ethnic work mode in Yunnan Province, it is surely of positive meaning for effective governance in other autonomous minority nationality areas in China. Based on such awareness, from view of political governance theories, on basis of Marxism national theories and policies, taking ethnic work practicei n Yunnan Province as specific cases, this paper aims to a trial explanation for the "good" ethnic work mode in autonomous minority nationality areas.

Impact of Multiple Satellite Ocean Color Samplings in a Day on Assessing Phytoplankton Dynamics

ZhongPing Lee,Mingshun Jiang,Curtiss Davis,Nima Pahlevan,안유환,Ronghua Ma 한국해양과학기술원 2012 Ocean science journal Vol.47 No.3

Ocean-color imagers on conventional polar-orbiting satellites have a revisit time of ~2 days for most regions, which is further reduced if the area is frequently cloudy. The Geostationary Ocean Color Imager (GOCI), the first ocean-color imager on a geostationary satellite, provides measurements 8times a day, thus significantly improving the frequency of measurements for studies of ocean environments. Here, we use results derived from GOCI measurements over Taihu Lake to demonstrate that the extra sampling can be used to improve the accuracy of statistically averaged longer-term (daily) measurements. Additionally, using numerical simulations, we demonstrate that the coupling of diurnal variations of both biomass and photosynthetic available radiation can improve the accuracy of daily primary production estimates. These results echo that higher sampling frequency can improve our estimates of longer-term dynamics of biogeochemical processes and highlights the value of ocean color measurements from geostationary satellites.

Incremental forming of hydraulic supports: a numerical and experimental study of thickness distribution

Miao Shang,Yan Li,Kuangyu Chen,Mingshun Yang,Xingbai Zhao,Kaixin Nie 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12

The incremental forming technology has enormous potential in manufacturing sheet parts. However, non-uniform thickness distribution is a common technical existing issue in forming thin-walled parts using this technology. The use of hydraulic supports has been a realistic, promising solution to this issue. Based on hydraulically-supported incremental forming technology, a variety of strategies were proposed in this article for hydrostatic and variable pressure supports respectively. For each supporting strategy, the thickness distribution and materials flow laws in various forming regions were explored. Experimental and simulation results indicate that when the hydraulic pressure is less than 1.8 bar, the thickness distribution of the hydrostatic-supports is significantly better than that of conventional incremental forming, and the thickness distribution of the variable-pressure supports may be better than that of the hydrostatic-support. The hydraulically-supported incremental forming technology has an potential of enhancing enhance the thickness distribution performance and forming performance of thin-walled parts.

Identification of Quantitative Trait Loci (QTLs) for Flowering Time Using SSR Marker in Maize Under Water Stress

Yannong Xiao,Xinhai Li,Shihuang Wang,Xiangdong Wang,Mingshun Li,Yonglian Zheng 한국유전학회 2004 Genes & Genomics Vol.26 No.4

The selection of reduction of anthesis-silking interval (ASI) in maize breeding is an efficient way to develop maize varieties more tolerant to dry growing conditions. Characterization of the quantitative trait loci (QTL) that controlled the flowering time will be helpful for selection in maize breeding. In this study, flowering time of individuals in a 234 F2:3 family, derived from the cross between inbred lines X178 and B73, was evaluated under well-watered and water-stressed conditions at the same location. SSR (microsatellite) was used to identify flowering time QTL. The results showed that the broad-sense heritability for male flowering time (MFT), female flowering time (FFT) and ASI were 0.72, 0.72 - 0.74 and 0.40 - 0.42, respectively, and ASI was significantly correlated to FFT. Under water-stressed condition, 9, 6 and 6 QTLs were identified for MFT, FFT and ASI, respectively, and individual QTL accounted for approximately 2.88% - 31.65% of the phenotypic variation. Some QTLs for MFT were mapped overlapping with those for FFT and ASI. One QTL on chromosome 9 (near nc134) had the strongest effect on MFT, FTT and ASI. It was suggested that the epistasis contributed to the phenotypic variation of flowering time.

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation

Zhengxia Wang,Ningfei Ji,Zhongqi Chen,Zhixiao Sun,Chaojie Wu,Wenqing Yu,Fan Hu,Mao Huang,Mingshun Zhang 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.5

Purpose: CD4+T cells are essential in the pathogenesis of allergic asthma. We have previously demonstrated that microRNA-1165-3p (miR-1165-3p) was significantly reduced in T-helper type (Th) 2 cells and that miR-1165-3p was a surrogate marker for atopic asthma. Little is known about the mechanisms of miR-1165-3p in the regulation of Th2-dominated allergic inflammation. We aimed to investigate the associations between Th2 differentiation and miR-1165b-3p in asthma as well as the possible mechanisms. Methods: CD4+ naïve T cells were differentiated into Th1 or Th2 cells in vitro. MiR-1165-3p was up-regulated or down-regulated using lentiviral systems during Th1/Th2 differentiation. In vivo, the lentiviral particles with the miR-1165-3p enhancer were administered by tail vein injection on the first day of a house dust mite -induced allergic airway inflammation model. Allergic inflammation and Th1/Th2 differentiation were routinely monitored. To investigate the potential targets of miR-1165-3p, biotin-microRNA pull-down products were sequenced, and the candidates were further verified with a dual-luciferase reporter assay. The roles of a target protein phosphatase, Mg2+/Mn2+-dependent 1A (PPM1A), in Th2 cell differentiation and allergic asthma were further explored. Plasma PPM1A was determined by ELISA in 18 subjects with asthma and 20 controls. Results: The lentivirus encoding miR-1165-3p suppressed Th2-cell differentiation in vitro. In contrast, miR-1165-3p silencing promoted Th2-cell development. In the HDM-induced model of allergic airway inflammation, miR-1165-3p up-regulation was accompanied by reduced airway hyper-responsiveness, serum immunoglobulin E, airway inflammation and Th2-cell polarization. IL-13 and PPM1A were the direct targets of miR-1165-3p. The expression of IL-13 or PPM1A was inversely correlated with that of miR-1165-3p. PPM1A regulated the signal transducer and activator of transcription and AKT signaling pathways during Th2 differentiation. Moreover, plasma PPM1A was significantly increased in asthmatic patients. Conclusions: MiR-1165-3p negatively may regulate Th2-cell differentiation by targeting IL-13 and PPM1A in allergic airway inflammation.

IL-33 Promotes ST2-Dependent Fibroblast Maturation via P38 and TGF-β in a Mouse Model of Epidural Fibrosis

Wang Haoran,Wu Tao,Hua Feng,Sun Jinpeng,Bai Yunfeng,Wang Weishun,Liu Jun,Zhang Mingshun 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3

BACKGROUND: Recent evidence suggests that IL-33, a novel member of the IL-1b family, is involved in organ fibrosis. However, the roles of IL-33 and its receptor ST2 in epidural fibrosis post spine operation remain elusive. METHODS: A mouse model of epidural fibrosis was established after laminectomy. IL-33 in the wound tissues post laminectomy was measured with Western blotting, ELISA and immunoflurosence imaging. The fibroblast cell line NIH- 3T3 and primary fibroblasts were treated with IL-33 and the mechanisms of maturation of fibroblasts into myofibroblasts were analyzed. To explore roles of IL-33 and its receptor ST2 in vivo, IL-33 knockout (KO) and ST2 KO mice were employed to construct the model of laminectomy. The epidural fibrosis was evaluated using H&E and Masson staining, western-blotting, ELISA and immunohistochemistry. RESULTS: As demonstrated in western blotting and ELISA, IL-33 was increased in epidural wound tissues post laminectomy. The immunoflurosence imaging revealed that endothelial cells (CD31?) and fibroblasts (a-SAM?) were major producers of IL-33 in the epidural wound tissues. In vitro, IL-33 promoted fibroblast maturation, which was blocked by ST2 neutralization antibody, suggesting that IL-33-promoted-fibroblasts maturation was ST2 dependent. Further, IL-33/ ST2 activated MAPK p38 and TGF-b pathways. Either p38 inhibitor or TGF-b inhibitor decreased fibronectin and a-SAM production from IL-33-treated fibroblasts, suggesting that p38 and TGF-b were involved with IL-33/ST2 signal pathways in the fibroblasts maturation. In vivo, IL-33 KO or ST2 KO decreased fibronectin, a-SMA and collagen deposition in the wound tissues of mice that underwent spine surgery. In addition, TGF-b1 was decreased in IL-33 KO or ST2 KO epidural wound tissues. CONCLUSION: In summary, IL-33/ST2 promoted fibroblast differentiation into myofibroblasts via MAPK p38 and TGFb in a mouse model of epidural fibrosis after laminectomy.

Molecular characterization of a Niemann-Pick disease type C2 protein from the honeybee Apis cerana

Lee, Kwang Sik,Park, Hee Geun,Yijie, Deng,Kim, Bo Yeon,Kyung, Seung Su,Choi, Yong Soo,Yoon, Hyung Joo,Li, Mingshun,Jin, Byung Rae 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3

Drosophila Niemann-Pick disease type C2 (NPC2) proteins play roles in sterol homeostasis, steroid biosynthesis, and innate immune signaling pathways. In this study, a bee (Apis cerana) NPC2a protein (AcNPC2a) that might function in innate immune reactions was identified. AcNPC2a consisted of 148 amino acids, which included six conserved cysteine residues. Recombinant AcNPC2a protein (expressed in baculovirus-infected insect cells) bound directly to live Escherichia coli, Bacillus thuringiensis, and Beauveria bassiana; however, AcNPC2a did not show antimicrobial activity against these microorganisms. Nevertheless, the expression of AcNPC2a was significantly induced in the fat body of A. cerana worker bees after injection with E. coli, B. thuringiensis, or B. bassiana. Our data suggest a role for AcNPC2a in innate immunity that is induced in response to microbial challenge and binds directly to the cell walls of bacteria and fungi. These findings provide insight into the role of AcNPC2a during the innate immune response following bacterial and fungal infection.

LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.